Abstract:
:Purine uptake has been studied in many protozoan parasites in the last few years, and several of the purine transporters have been cloned. In contrast, very little is known about the salvage of preformed pyrimidines by protozoa, and no pyrimidine transporters have been cloned, yet chemotherapy based on pyrimidine nucleobases and nucleosides has been as effective as purine antimetabolites in the treatment of infectious and neoplastic disease. Here, we surveyed the presence of pyrimidine transporters in Trypanosoma brucei brucei. We could not detect any mediated uptake of thymine, thymidine or cytidine, but identified a very high-affinity transporter for cytosine, designated C1, with a K(m) value of 0.048+/-0.009 microM. We also confirmed the presence of the previously reported U1 uracil transporter and found it capable of mediating uridine uptake as well, with a K(m) of 33+/-5 microM. A higher-affinity U2 uridine transporter (K(m)=4.1+/-2.1 microM) was also identified, but efficiency of the C1 and U2-mediated transport was low. Pyrimidine antimetabolites were tested as potential trypanocidal agents and only 5-fluorouracil was found to be effective. This drug was efficiently taken up by bloodstream forms of T. b. brucei.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Gudin S,Quashie NB,Candlish D,Al-Salabi MI,Jarvis SM,Ranford-Cartwright LC,de Koning HPdoi
10.1016/j.exppara.2006.02.018subject
Has Abstractpub_date
2006-10-01 00:00:00pages
118-25issue
2eissn
0014-4894issn
1090-2449pii
S0014-4894(06)00064-6journal_volume
114pub_type
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
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abstract::Okuda, K., Esteva, M., Segura, E. L., and Bijovsky, A. T. 1999. The cytostome of Trypanosoma cruzi epimastigotes is associated with the flagellar complex. Experimental Parasitology 92, 223-231. Proliferative forms of Trypanosoma cruzi, amastigotes and epimastigotes, have a cytostome, a specialized structure formed by ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1999.4419
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journal_title:Experimental parasitology
pub_type: 杂志文章
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doi:10.1016/j.exppara.2012.02.006
更新日期:2012-04-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2006.04.010
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
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更新日期:2015-12-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:1987-02-01 00:00:00
abstract:AIM:The present study investigated the processes of macrogametogenesis and oocyst formation of Eimeria tenella (Xiamen strain), including the formation of wall-forming body1 (WFB1) and wall-forming body 2 (WFB2), the club-shape body and the origin of the residual body during the transformation from a macrogamete to an ...
journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:2006-07-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:2016-09-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2007.07.013
更新日期:2008-02-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2010.08.021
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2012.01.005
更新日期:2012-04-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2004.11.001
更新日期:2005-01-01 00:00:00
abstract::Serine proteases have been identified as important molecules that are involved in many parasitic infections, and these molecules have also been suggested to play important roles in Trichinella spiralis infections. In the present study, the antigenic serine protease gene Ts-ADSp-7, which was screened from a cDNA librar...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2018.09.009
更新日期:2018-12-01 00:00:00
abstract::Protein tyrosine phosphorylation (PY) is a well-known signalling mechanism which is also involved in host-parasite interactions. Despite its transcendence, PY has been poorly studied in parasitic helminths. The aim of this study is to examine the effect of bile salts on the PY pattern in parasitic trematodes. Two dist...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2004.01.006
更新日期:2004-01-01 00:00:00