Selective killing of Leishmania amastigotes expressing a thymidine kinase suicide gene.

abstract：:Chagas disease is characterized by cardiac lesions and a high level of PGE2. Our objective was to investigate the role of PGE2 in cardiac lesions. BALB/c mice were infected with Trypanosoma cruzi (1x10(3) trypomastigote forms) and were treated daily with PBS, meloxicam (0.5 mg/kg) or etoricoxib (0.6 mg/kg). The animal...

journal_title：Experimental parasitology

authors： Abdalla GK,Faria GE,Silva KT,Castro EC,Reis MA,Michelin MA

更新日期：2008-04-01 00:00:00

abstract：:Extramedullar proliferation of eosinophil granulocytes can be induced in mice chronically infected with Schistosoma mansoni, by intraperitoneal implants of glass coverslips. Immature eosinophils are located in discrete foci on glass implants; they are not correlated with the eosinophil population of the peritoneal cav...

journal_title：Experimental parasitology

authors： Borojevic R,Cury El-Cheikh MC,Nicola MH

更新日期：1986-12-01 00:00:00

abstract：:Excystation of Giardia lamblia entails differentiation of dormant cysts into parasitic trophozoites. Despite its importance for infection, this transformation is not understood at the cellular or molecular levels. In these studies, we report that excystation entails detection of environmental stimuli across the tough ...

journal_title：Experimental parasitology

authors： Hetsko ML,McCaffery JM,Svärd SG,Meng TC,Que X,Gillin FD

更新日期：1998-03-01 00:00:00

abstract：:There is evidence which suggests that malaria sporozoites recognize mosquito salivary glands by specific receptor-ligand interactions. We are interested in identifying the putative salivary gland receptor(s) for sporozoite invasion. We used an in vivo bioassay for sporozoite invasion of salivary glands. In this assay,...

journal_title：Experimental parasitology

authors： Barreau C,Touray M,Pimenta PF,Miller LH,Vernick KD

更新日期：1995-11-01 00:00:00

abstract：:The aim of this study was to develop a simple and reliable method to determine the viability of Giardia intestinalis after incubation with an anti-giardial agent by using a colorimetric method. Factors that may affect the optical density value were systematically evaluated. The most suitable conditions were obtained w...

journal_title：Experimental parasitology

authors： Hounkong K,Sawangjaroen N,Phongpaichit S

更新日期：2011-02-01 00:00:00

abstract：:One- and two-dimensional gel electrophoresis of the solubilized mitochondrial proteins of bloodstream and procyclic trypomastigote Trypanosoma brucei rhodesiense and radiolabeling of proteins in the presence of cycloheximide were used to identify proteins synthesized in the trypanosome mitochondrion. The proteins whic...

journal_title：Experimental parasitology

authors： Le Febvre RB,Hill GC

更新日期：1986-08-01 00:00:00

abstract：:Giardia lamblia undergo surface antigenic variation in vitro and in vivo. The presence of variant trophozoites can be detected in clones after exposure to cytotoxic monoclonal antibodies. Surviving Giardia (progeny) no longer possess the initial major surface antigen which is replaced by new antigens. Exposure of a cl...

journal_title：Experimental parasitology

authors： Nash TE

更新日期：1989-02-01 00:00:00

abstract：:Tc-85, an 85-kDa surface glycoprotein specific for the trypomastigote stage of Trypanosoma cruzi, has been implicated in the invasion of host cells by the parasite. Radioactive palmitic acid was incorporated into Tc-85 immunoprecipitated from the culture medium with the H1A10 monoclonal antibody, suggesting that shedd...

journal_title：Experimental parasitology

authors： Abuin G,Couto AS,de Lederkremer RM,Casal OL,Galli C,Colli W,Alves MJ

更新日期：1996-04-01 00:00:00

abstract：:We show that a purified preparation of the prominent tartrate-resistant acid phosphatase (E.C.3.1.3.2), isolated from the external surface of the intracellular parasite Leishmania donovani (promastigote form), inhibits toxic oxidative metabolite production of neutrophils. Preincubation of a neutrophil suspension (2.5 ...

journal_title：Experimental parasitology

authors： Remaley AT,Glew RH,Kuhns DB,Basford RE,Waggoner AS,Ernst LA,Pope M

更新日期：1985-12-01 00:00:00

abstract：PURPOSE:Praziquantel (PZQ) is the conventional antibilharzial agent. Nevertheless, no antibilharzial prophylactic agents or 100% curable therapy approved and no reported data about use of human CD34+ Umbilical Cord Blood Stem Cells (CD34+UCBSCs) or Wharton Jelly Mesenchymal Stem Cells (WJMSCs) in prevention and/or comp...

journal_title：Experimental parasitology

authors： Magdy M,Fahmy A,Zaki NI,Mohamed AK

更新日期：2020-10-01 00:00:00

abstract：:The reactivity to overlapping decapeptides based on sequences of human band 3 protein was determined for 24 rabbit serum samples. A high percentage of the sera recognized sequences of amino acid 650-670, as well as several other sequences representing putative exofacial regions of the band 3 protein. When sera were us...

journal_title：Experimental parasitology

authors： Crandall I,Guthrie N,Sherman IW

更新日期：1996-01-01 00:00:00

abstract：:The compound 2-hydroxy-3-(1'-propen-3-phenyl)-1,4-naphthoquinone (PHNQ6) was evaluated for activity against Toxoplasma gondii, alone or combined with sulfadiazine. Treatment with PHNQ6 combined with sulfadiazine protected at least 70 and 90% of mice infected with RH and EGS strains, respectively. Mice were treated wit...

journal_title：Experimental parasitology

authors： Ferreira RA,Oliveira AB,Ribeiro MF,Tafuri WL,Vitor RW

更新日期：2006-06-01 00:00:00

abstract：:Human cysticercosis caused by Taenia crassiceps is unusual; however, it is an useful experimental model for cysticercosis studies. Benzimidazole derivatives are important antihelminthic drugs widely used against helminths. A novel compound 6-chloro-5-(1-naphthyloxy) -2-(trifluoromethyl)-1H-benzimidazole (RCB20) is a b...

journal_title：Experimental parasitology

authors： Fraga CM,da Costa TL,de Castro AM,Reynoso-Ducoing O,Ambrosio J,Hernández-Campos A,Castillo R,Vinaud MC

更新日期：2017-01-01 00:00:00

abstract：:To understand the genetic basis of virulence, gene expression profiles of a temperature-sensitive clone (NCts-8, relatively avirulent) and its wild type (NC-1) of Neospora caninum were characterized and compared using a high-density microarray with approximately 63,000 distinct oligonucleotides. This microarray consis...

journal_title：Experimental parasitology

authors： Li RW,Tuo W

更新日期：2011-12-01 00:00:00

abstract：:Toxoplasma gondii is an opportunistic zoonotic protozoan that exceeds neurological and congenital impact sequence to reactivating latent toxoplasmosis especially under immunosuppression. Sex-associated hormones influence the severity of Toxoplasma infection. Thus, our study aimed to compare toxoplasmosis associated mo...

journal_title：Experimental parasitology

authors： Hegazy MM,Elmehankar MS,Azab MS,El-Tantawy NL,Abdel-Aziz A

更新日期：2019-07-01 00:00:00

abstract：:Studies have shown that symptomatic infection by Giardia lamblia causes acute or chronic diarrhea, dehydration, abdominal pain and malabsorption, leading to undernutrition and weight loss. The aim of the present study was to evaluate the effects of giardiasis and its combination with a low-protein diet on the intestin...

journal_title：Experimental parasitology

authors： Gomes MA,de Oliveira DR,de Freitas SE,de Pinho Viana M,Borges EL

更新日期：2012-08-01 00:00:00

abstract：:Axenic culture of Leishmania is generally performed in rich, serum-supplemented media which sustain robust growth over multiple passages. The use of such undefined media, however, obscures proteomic analyses and confounds the study of metabolism. We have established a simple, defined culture medium that supports the s...

journal_title：Experimental parasitology

authors： Nayak A,Akpunarlieva S,Barrett M,Burchmore R

更新日期：2018-02-01 00:00:00

abstract：:A thrombin (E.C. 3.4.21.5) inhibitor, savignin, was isolated from the salivary glands of Ornithodoros savignyi by a combination of size exclusion, anion-exchange, and reversed-phase chromatography. The inhibitor has a molecular mass of 12,430.4 Da as determined by electrospray mass spectrometry. The behavior of savign...

journal_title：Experimental parasitology

authors： Nienaber J,Gaspar AR,Neitz AW

更新日期：1999-10-01 00:00:00

abstract：:In this report, an immunodominant antigen called BgIRA from Babesia gibsoni is identified and described. A highly repetitive antigen was screened from a cDNA library. The genomic BgIRA gene exists as single cope gene and contains 10 introns. BgIRA plays a dominant role in the immune response in dogs infected with B. g...

journal_title：Experimental parasitology

authors： Jia H,Terkawi MA,Aboge GO,Goo YK,Zhou J,Lee EG,Nishikawa Y,Igarashi I,Fujisaki K,Xuan X

更新日期：2008-01-01 00:00:00

abstract：:The oxidative metabolism of peritoneal macrophages (PM) and neutrophils from nifedipine (calcium channel blocker)-treated, Plasmodium berghei (NK 65)-infected and normal infected Swiss Albino mice was studied. A significant fall in oxidative metabolism as evidenced by decreased chemiluminescence (CL) response (P less ...

journal_title：Experimental parasitology

authors： Kalra A,Dubey ML,Ganguly NK,Mohan K,Mahajan RC

更新日期：1992-02-01 00:00:00

abstract：:We performed a calibration of flotation in tube, McMaster and FLOTAC to determine the optimal flotation solution (FS) and the influence of faecal preservation for the diagnosis of Ancylostoma caninum in dogs, and compared the accuracy of the three copromicroscopic techniques. Among nine different FS, sodium chloride a...

journal_title：Experimental parasitology

authors： Cringoli G,Rinaldi L,Maurelli MP,Morgoglione ME,Musella V,Utzinger J

更新日期：2011-05-01 00:00:00

abstract：:Recent epidemiological evidences revealed the higher prevalence of 'O' blood group in the residents of malaria-endemic areas. Also some data indicated preference of mosquitoes to 'O' group. The aim of this study was to determine ABO group ratio in the residents as well as ABO group preference of Anopheles in two malar...

journal_title：Experimental parasitology

authors： Anjomruz M,Oshaghi MA,Sedaghat MM,Pourfatollah AA,Raeisi A,Vatandoost H,Mohtarami F,Yeryan M,Bakhshi H,Nikpoor F

更新日期：2014-01-01 00:00:00

abstract：:Okuda, K., Esteva, M., Segura, E. L., and Bijovsky, A. T. 1999. The cytostome of Trypanosoma cruzi epimastigotes is associated with the flagellar complex. Experimental Parasitology 92, 223-231. Proliferative forms of Trypanosoma cruzi, amastigotes and epimastigotes, have a cytostome, a specialized structure formed by ...

journal_title：Experimental parasitology

authors： Okuda K,Esteva M,Segura EL,Bijovsy AT

更新日期：1999-08-01 00:00:00

abstract：:Rabbit coccidiosis caused by members of the genus Eimeria can cause enormous economic impact worldwide, but the genetics, epidemiology and biology of these parasites remain poorly understood. In the present study, we sequenced and annotated the complete mitochondrial (mt) genomes of five Eimeria species that commonly ...

journal_title：Experimental parasitology

authors： Liu GH,Tian SQ,Cui P,Fang SF,Wang CR,Zhu XQ

更新日期：2015-12-01 00:00:00

abstract：:Propylene glycol used as an ophthalmic demulcent in certain contact-lens care systems has been included recently among factors responsible for increasing Acanthamoeba keratitis. In this study, we provide evidence that propylene glycol as well as examined contact-lens solutions containing it induce rapid differentiatio...

journal_title：Experimental parasitology

authors： Kliescikova J,Kulda J,Nohynkova E

更新日期：2011-01-01 00:00:00

abstract：:Insects manifest effective immune responses that include both cellular and humoral components. Morphological and quantitative aspects of cellular and humoral cooperation during nodule formation in Chrysomya megacephala hemolymph against Saccharomyces cerevisae yeast cells were demonstrated for the first time. The anal...

journal_title：Experimental parasitology

authors： Faraldo AC,Gregório EA,Lello E

更新日期：2008-03-01 00:00:00

abstract：:We describe the characterization of Tc38, a Trypanosoma cruzi gene coding for a 337-amino-acid protein with a predicted molecular mass of 38 kDa. Tc38 presents similarities to the plant storage vacuolar protein gamma-3-hordein involved in the transport and targeting of prolamins to the vacuole of developing barley end...

journal_title：Experimental parasitology

authors： Robello C,Dallagiovanna B,Castanys S,Gamarro F,Ehrlich R

更新日期：2000-02-01 00:00:00

abstract：:Three studies were conducted to examine the function of ecdysteroids in the development of parasitic nematodes. Ecdysone and 20-hydroxyecdysone were extracted, separated chromatographically, and measured in the reproductive tracts of adult female Ascaris suum. Perienteric fluid and the body wall did not contain measur...

journal_title：Experimental parasitology

authors： Fleming MW

更新日期：1985-10-01 00:00:00

abstract：:Polymerase chain reaction amplification of several polymorphic genes has been used to study the population biology of Plasmodium falciparum. S antigen is particularly suitable for such studies, but difficulties in the amplification of this gene have precluded its use. Here we describe a simple method for the amplifica...

journal_title：Experimental parasitology

authors： Kyes S,Craig AG,Marsh K,Newbold CI

更新日期：1993-12-01 00:00:00

abstract：:The experiments described here report on the correlation between the ex vivo diffusion of different benzimidazole (BZD) anthelmintics into the cestode parasite Moniezia benedeni, and their octanol-water partition coefficients (P.C.). The characterisation of the drug diffusion process into target parasites is relevant ...

journal_title：Experimental parasitology

authors： Mottier ML,Alvarez LI,Pis MA,Lanusse CE

更新日期：2003-01-01 00:00:00