当前位置： SCI文献检索 > EXPERIMENTAL PARASITOLOGY期刊下所有文献 > Acanthamoeba culbertsoni isolated from a clinical case with intraocular dissemination: Structure and in vitro analysis of the interaction with hamster cornea and MDCK epithelial cell monolayers.

Acanthamoeba culbertsoni isolated from a clinical case with intraocular dissemination: Structure and in vitro analysis of the interaction with hamster cornea and MDCK epithelial cell monolayers.

Abstract：

:Acanthamoeba culbertsoni trophozoites, previously isolated from a human keratitis case with severe intraocular damage, were maintained in axenic culture. Co-incubation of amoebae with MDCK cell monolayers demonstrated an apparent preference of the amoebae to introduce themselves between the cells. The trophozoites appeared to cross the cell monolayer through the tight junctions, which resulted in decreased trans-epithelial resistance (TER) measurements. Unexpectedly, after co-incubation of amoebae with hamster corneas, we observed that the trophozoites were able to cross the different cell layers and reach the corneal stroma after only 12 h of interaction, in contrast to other Acanthamoeba species. These observations suggest that this A. culbertsoni isolate is particularly pathogenic. Further research with diverse methodologies needs to be performed to explain the unique behavior of this Acanthamoeba strain.

journal_name

Exp Parasitol

journal_title

Experimental parasitology

authors

González-Robles A,Omaña-Molina M,Salazar-Villatoro L,Flores-Maldonado C,Lorenzo-Morales J,Reyes-Batlle M,Arnalich-Montiel F,Martínez-Palomo A

doi

10.1016/j.exppara.2017.09.018

subject

Has Abstract

pub_date

2017-12-01 00:00:00

pages

245-253

eissn

0014-4894

issn

1090-2449

pii

S0014-4894(17)30416-2

journal_volume

183

pub_type

杂志文章

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