Antibody response in dogs experimentally infected with Leishmania infantum: infection course antigen markers.


:Five-month-old beagle dogs were experimentally infected with amastigotes of Leishmania infantum and kept for 14 months. Infection course was monitored by clinical examination, serum protein variations, and levels of specific antibodies against Leishmania estimated by indirect immunofluorescence test and Western blotting (WB). Infected animals developed notable changes in serum protein levels reaching maximum protein concentrations 2-3 months postinfection (p.i.) related to the gamma-globulin fraction. Specific antibody titers were in good agreement with the serum protein rise, reaching immunofluorescence values of over 1:800 3 months p.i. Serial Western blotting analysis with L. infantum promastigotes protein showed a strong response against immunodominant antigens of 50-57, 42, and 29 kDa during most of the studied period with immunofluorescence titers of over 1:100 and in addition the response was remarkably homogeneous among the infected dogs. Immunoreactivity patterns displayed time-related variations; the response against 29 and 50-57 kDa was seen very early, followed by the reaction around 42, 76, and 86 kDa. In addition the recognition of peptides around 34-35.4 and 26 kDa was restricted to the acute phase of the experimental infection. Preliminary results obtained in naturally infected dogs seem to support the predictive value of the WB.


Exp Parasitol


Carrera L,Fermín ML,Tesouro M,García P,Rollán E,González JL,Méndez S,Cuquerella M,Alunda JM




Has Abstract


1996-03-01 00:00:00














  • Comparative glucose utilization rates in separated and mated schistosomes.

    abstract::The rate of phosphorylation of 2-deoxyglucose (2DG) was determined by sequential pulsing of schistosomes (Schistosoma mansoni, S. japonicum, and S. haematobium) with 3H- and 14C-labeled 2-deoxy-D-glucose. Subsequent column chromatographic separation of the neutral [3H]2DG and [14C]2DG from the 3H- and 14C-labeled 2-de...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Cornford EM,Fitzpatrick AM

    更新日期:1987-12-01 00:00:00

  • Identification and characterization of a Giardia lamblia group-specific gene.

    abstract::Giardia lamblia consist of heterogeneous isolates that can be divided into at least three groups. Differential screening of a cDNA library with isolate-specific antisera identified a gene which is expressed and found only in Group 3 isolates. This gene, GLORF-C4, is 597 bp in length and predicts a deduced protein of 1...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Nash TE,Mowatt MR

    更新日期:1992-12-01 00:00:00

  • Ornithodorus tartakovskyi: quantitation and ultrastructure of cutaneous basophil responses in the guinea pig.

    abstract::Cutaneous lesions elicited in guinea pigs by primary and secondary feeding populations of the argasid tick, Ornithodorus tartakovskyi, were analyzed by light and electron microscopy. Small clusters of basophils appeared at primary bite sites within 24 hr of tick attachment, and by 72 hr constituted approximately 11% o...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: McLaren DJ,Worms MJ,Brown SJ,Askenase PW

    更新日期:1983-10-01 00:00:00

  • Anti-Giardia activity of Syzygium aromaticum essential oil and eugenol: effects on growth, viability, adherence and ultrastructure.

    abstract::The present work evaluates the anti-Giardia activity of Syzygium aromaticum and its major compound eugenol. The effects were evaluated on parasite growth, adherence, viability and ultrastructure. S. aromaticum essential oil (IC(50)=134 μg/ml) and eugenol (IC(50)=101 μg/ml) inhibited the growth of G. lamblia. The essen...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Machado M,Dinis AM,Salgueiro L,Custódio JB,Cavaleiro C,Sousa MC

    更新日期:2011-04-01 00:00:00

  • ABO blood groups of residents and the ABO host choice of malaria vectors in southern Iran.

    abstract::Recent epidemiological evidences revealed the higher prevalence of 'O' blood group in the residents of malaria-endemic areas. Also some data indicated preference of mosquitoes to 'O' group. The aim of this study was to determine ABO group ratio in the residents as well as ABO group preference of Anopheles in two malar...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Anjomruz M,Oshaghi MA,Sedaghat MM,Pourfatollah AA,Raeisi A,Vatandoost H,Mohtarami F,Yeryan M,Bakhshi H,Nikpoor F

    更新日期:2014-01-01 00:00:00

  • Philasterides dicentrarchi, a histophagous ciliate causing scuticociliatosis in olive flounder, Philasterides dicentrarchi--histopathology investigations.

    abstract::Philasterides dicentrarchi is a histophagous scuticociliate infecting Korean olive flounder farms in Jeju Island, South Korea, where it causes significant economic losses. However, the route of entry of these parasites in olive flounder is currently unknown. In the present study, we attempted experimental infection wi...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Harikrishnan R,Jin CN,Kim JS,Balasundaram C,Heo MS

    更新日期:2012-03-01 00:00:00

  • Development of a molecular xenomonitoring protocol to assess filariasis transmission.

    abstract::According to the World Health Organization, lymphatic filariasis (LF), a mosquito-borne neglected tropical disease (NTD), should be eliminated as a public health concern by the end of 2020. To this end, the goals of the Global Programme to Eliminate Lymphatic Filariasis (GPELF) include interrupting transmission throug...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Albuquerque AL,Araújo TA,Melo DCTV,Paiva MHS,Melo FL,Oliveira CMF,Ayres CFJ

    更新日期:2020-08-01 00:00:00

  • Schistosoma mansoni: possible involvement of protein kinase C in linoleic acid-induced proteolytic enzyme release from cercariae.

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    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Matsumura K,Mitsui Y,Sato K,Sakamoto M,Aoki Y

    更新日期:1991-04-01 00:00:00

  • Low levels of mammalian TGF-beta1 are protective against malaria parasite infection, a paradox clarified in the mosquito host.

    abstract::Nitric oxide (NO), derived from catalysis of inducible NO synthase (iNOS), limits malaria parasite growth in mammals. Transforming growth factor (TGF)-beta1 suppresses iNOS in cells in vitro as well as in vivo in mice, but paradoxically severe malaria in humans is associated with low levels of TGF-beta1. We hypothesiz...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Luckhart S,Lieber MJ,Singh N,Zamora R,Vodovotz Y

    更新日期:2008-02-01 00:00:00

  • Characterization and cellular distribution of heat-shock proteins HSP70 and HSP60 in Trypanosoma cruzi.

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    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Giambiagi-deMarval M,Souto-Padrón T,Rondinelli E

    更新日期:1996-08-01 00:00:00

  • Hymenolepis diminuta: analysis of the expression of Toll-like receptor genes and protein (TLR3 and TLR9) in the small and large intestines of rats.

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    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Kosik-Bogacka DI,Wojtkowiak-Giera A,Kolasa A,Baranowska-Bosiacka I,Lanocha N,Wandurska-Nowak E,Izabela G,Salamatin R,Jagodzinski PP

    更新日期:2014-10-01 00:00:00

  • Host species diversity and post-blood feeding carbohydrate availability enhance survival of females and fecundity in Aedes albopictus (Diptera: Culicidae).

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    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Xue RD,Ali A,Barnard DR

    更新日期:2008-06-01 00:00:00

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    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Parvathi A,Sanath Kumar H,Kenchanna Prakasha B,Lu J,Xu X,Hu W,Feng Z,Karunasagar I,Karunasagar I

    更新日期:2007-03-01 00:00:00

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    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Dodd NS,Lord JS,Jehle R,Parker S,Parker F,Brooks DR,Hide G

    更新日期:2014-04-01 00:00:00

  • Nematospiroides dubius: genetic control of immunity to infections of mice.

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    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Enriquez FJ,Zidian JL,Cypess RH

    更新日期:1988-10-01 00:00:00

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    pub_type: 杂志文章


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    更新日期:2011-09-01 00:00:00

  • Thermal properties of red blood cells infected by malaria parasites.

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    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Forte T,Roca L

    更新日期:1989-10-01 00:00:00

  • Phylogenetic position of Leishmania isolates from Khyber Pakhtunkhwa province of Pakistan.

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    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Khan NH,Messenger LA,Wahid S,Sutherland CJ

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  • Trypanosoma cruzi: effects of azadirachtin and ecdysone on the dynamic development in Rhodnius prolixus larvae.

    abstract::The effects of azadirachtin and ecdysone on the Trypanosoma cruzi population in the Rhodnius prolixus gut were investigated. T. cruzi were rarely found in the gut compartments of azadirachtin-treated larvae. High parasite numbers were observed in the stomach of the control and ecdysone groups until 10 days after treat...

    journal_title:Experimental parasitology

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    authors: Cortez MR,Provençano A,Silva CE,Mello CB,Zimmermann LT,Schaub GA,Garcia ES,Azambuja P,Gonzalez MS

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  • Experimental onchocerciasis in chimpanzees. Antibody response and antigen recognition after primary infection with Onchocerca volvulus.

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    journal_title:Experimental parasitology

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    authors: Soboslay PT,Weiss N,Dreweck CM,Taylor HR,Brotman B,Schulz-Key H,Greene BM

    更新日期:1992-06-01 00:00:00

  • Characterization of glycosomal RING finger proteins of trypanosomatids.

    abstract::The glycosomes of trypanosomatids are essential organelles that are evolutionarily related to peroxisomes of other eukaryotes. The peroxisomal RING proteins-PEX2, PEX10 and PEX12-comprise a network of integral membrane proteins that function in the matrix protein import cycle. Here, we describe PEX10 and PEX12 in Tryp...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Saveria T,Kessler P,Jensen BC,Parsons M

    更新日期:2007-05-01 00:00:00

  • Anti-Trypanosoma activity of bioactive metabolites from Photorhabdus luminescens and Xenorhabdus nematophila.

    abstract::Only two drugs are currently available for the treatment of Chagas disease and their effectiveness are unsatisfactory. Photorhabdus luminescens and Xenorhabdus nematophila, two enteric bacteria highly pathogenic to a broad range of insects, have been studied as potential source for bioactive metabolites against protoz...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Antonello AM,Sartori T,Silva MB,Prophiro JS,Pinge-Filho P,Heermann R,da Silva OS,Romão PRT

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  • Leishmania sp: comparative study with Toxoplasma gondii and Trypanosoma cruzi in their ability to initialize IL-12 and IFN-gamma synthesis.

    abstract::We compared in vitro and in vivo induction of IL-12 (p40) and IFN-gamma by mouse cells stimulated with Toxoplasma gondii, Trypanosoma cruzi, and different species of Leishmania. Spleen cells cultured in vitro with T. cruzi or T. gondii, but not with Leishmania, produced IL-12 (p40) and IFN-gamma. Accordingly, IL-12 (p...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Oliveira MA,Santiago HC,Lisboa CR,Ceravollo IP,Trinchieri G,Gazzinelli RT,Vieira LQ

    更新日期:2000-06-01 00:00:00

  • Schistosoma mansoni: influence of infection on mouse behavior.

    abstract::Schistosoma mansoni infection in humans and animals induces abnormal neurobehavioral responses following granuloma formation. In mice, granulomas in the liver are observed 8 weeks after infection, while after 15-20 weeks, the presence of eggs and granulomas in the brain has been reported. In this study, outbred CD-1 f...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Fiore M,Moroni R,Alleva E,Aloe L

    更新日期:1996-06-01 00:00:00

  • Non-classic characteristics define prominent DNase activities from the intestine and other tissues of Haemonchus contortus.

    abstract::The anthelmintic fenbendazole (FBZ) induces nuclear DNA fragmentation (DF) in intestinal cells of Haemonchus contortus. The DNA fragments had 3'-OH, which suggests involvement of a neutral DNase. To identify candidate DNase(s) involved, DNase activity in H. contortus intestine and other worm fractions was characterize...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Kwak D,Jasmer DP

    更新日期:2003-07-01 00:00:00

  • Antileishmanial activity and mechanism of action from a purified fraction of Zingiber officinalis Roscoe against Leishmania amazonensis.

    abstract::In recent years, considerable attention has been given to identify new antileishmanial products derived from medicinal plants, although, to date, no new effective compound has been recently applied to treat leishmaniasis. In the present study, the antileishmanial activity of a water extract from Zingiber officinalis R...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Duarte MC,Tavares GS,Valadares DG,Lage DP,Ribeiro TG,Lage LM,Rodrigues MR,Faraco AA,Soto M,da Silva ES,Chávez Fumagalli MA,Tavares CA,Leite JP,Oliveira JS,Castilho RO,Coelho EA

    更新日期:2016-07-01 00:00:00

  • Entamoeba histolytica: cytopathogenicity and lectin activity of avirulent mutants.

    abstract::Three clones of Entamoeba histolytica (L-6, C93, C919) were isolated by mutagenesis with ethyl methanesulfonate from the axenic strain HM1:IMSS and were studied for adherence, cytolytic, and soluble galactose inhibitable lectin activity. Avirulent clones adhered to and killed fewer Chinese hamster ovary cells than HM1...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Orozco E,Rodriquez MA,Murphy CF,Salata RA,Petri WA,Smith RD,Ravdin JI

    更新日期:1987-04-01 00:00:00

  • Schistosoma mansoni: long-term maintenance of clones by microsurgical transplantation of sporocysts.

    abstract::Schistosoma mansoni sporocysts originally derived from monomiracidially infected Biomphalaria glabrata snails were serially transplanted into the cephalopedal sinus of anesthetized snails by the microsurgical implantation of fragments of parasitized hepatopancreas and ovotestis. Three to six passages each of five male...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Cohen LM,Eveland LK

    更新日期:1984-02-01 00:00:00

  • Haemonchus contortus: in vitro and in vivo anthelmintic activity of aqueous and hydro-alcoholic extracts of Hedera helix.

    abstract::In vitro anthelmintic activity of crude extracts of the ripe fruits of Hedera helix was investigated on eggs and adult nematode parasites Haemonchus contortus. Aqueous extract of H. helix was also evaluated for in vivo anthelmintic activity at dose of 1.13 and 2.25 g/kg in sheep artificially infected with H. contortus...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Eguale T,Tilahun G,Debella A,Feleke A,Makonnen E

    更新日期:2007-08-01 00:00:00

  • Toxoplasma gondii: a simple high-throughput assay for drug screening in vitro.

    abstract::Toxoplasma gondii is the etiologic agent of toxoplasmosis. Although the combination of sulfadiazine and pyrimethamine is used as therapy for this disease, these drugs can have serious side effects and its use is limited in pregnancy. Therefore there is a need for new anti-T. gondii drugs in the clinic. Some systems fo...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Jin C,Kaewintajuk K,Jiang J,Jeong W,Kamata M,Kim HS,Wataya Y,Park H

    更新日期:2009-02-01 00:00:00