Abstract：
:The risk of human hookworm infections from land application of wastewater matrices could be high in regions with high hookworm prevalence. A rapid, sensitive and specific hookworm detection method from wastewater matrices is required in order to assess human health risks. Currently available methods used to identify hookworm ova to the species level are time consuming and lack accuracy. In this study, a real-time PCR method was developed for the rapid, sensitive and specific detection of canine hookworm (Ancylostoma caninum) ova from wastewater matrices. A. caninum was chosen because of its morphological similarity to the human hookworm (Ancylostoma duodenale and Necator americanus). The newly developed PCR method has high detection sensitivity with the ability to detect less than one A. caninum ova from 1 L of secondary treated wastewater at the mean threshold cycle (CT) values ranging from 30.1 to 34.3. The method is also able to detect four A. caninum ova from 1 L of raw wastewater and from ∼4 g of treated sludge with mean CT values ranging from 35.6 to 39.8 and 39.8 to 39.9, respectively. The better detection sensitivity obtained for secondary treated wastewater compared to raw wastewater and sludge samples could be attributed to sample turbidity. The proposed method appears to be rapid, sensitive and specific compared to traditional methods and has potential to aid in the public health risk assessment associated with land application of wastewater matrices. Furthermore, the method can be adapted to detect other helminth ova of interest from wastewater matrices.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Gyawali P,Sidhu JP,Ahmed W,Jagals P,Toze Sdoi
10.1016/j.exppara.2015.08.009subject
Has Abstractpub_date
2015-12-01 00:00:00pages
5-12eissn
0014-4894issn
1090-2449pii
S0014-4894(15)30026-6journal_volume
159pub_type
杂志文章abstract：:Toxoplasma gondii is a protozoan parasite of great importance in human and veterinary health. The frontline treatment of antifolates suffers a variety of drawbacks, including toxicity and allergic reactions, underscoring the need to identify novel drug targets for new therapeutics to be developed. We previously showed...
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abstract：:Interleukin-13 (IL-13) is a powerful anti-inflammatory cytokine that was previously shown to be a susceptibility factor for Leishmania major (L. major) infection. In this study we report a different role for IL-13 in rats infected with L. major; rIL-13 stimulates expression of pro-inflammatory cytokines and IL-12 whic...
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journal_title：Experimental parasitology
pub_type： 杂志文章,评审
doi：10.1016/0014-4894(89)90104-5
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abstract：:The rate of phosphorylation of 2-deoxyglucose (2DG) was determined by sequential pulsing of schistosomes (Schistosoma mansoni, S. japonicum, and S. haematobium) with 3H- and 14C-labeled 2-deoxy-D-glucose. Subsequent column chromatographic separation of the neutral [3H]2DG and [14C]2DG from the 3H- and 14C-labeled 2-de...
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(92)90048-f
更新日期：1992-03-01 00:00:00
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.2001.4651
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/s0014-4894(85)80026-6
更新日期：1985-08-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(90)90109-p
更新日期：1990-04-01 00:00:00
abstract：:Giardia lamblia is the most commonly detected parasite in the intestinal tract of humans and other mammals causing giardiasis. Giardia presents several cytoskeletal structures with microtubules as major components such as the ventral adhesive disk, eight flagella axonemes, the median body and funis. Many drugs have al...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2005.01.007
更新日期：2005-05-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：2015-02-01 00:00:00
abstract：:Rhipicephalus microplus, the cattle tick, is a major cause of economic losses in bovine production. Due to the widespread acaricidal resistance to commercially available products, as well as their toxicity and environmental impact, alternative control methods are required. Nanoformulations produced from plant extracts...
journal_title：Experimental parasitology
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doi：10.1016/j.exppara.2020.107919
更新日期：2020-08-01 00:00:00
abstract：:The diagnostic potential of recombinant E/S antigens of the lymphatic filaria Brugia malayi was investigated by Western blot. A cDNA expression library was constructed using B. malayi male adult worm mRNA, and E/S recombinants were identified with a rabbit antiserum raised against E/S products collected in vitro from ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1994.1110
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(91)90066-6
更新日期：1991-11-01 00:00:00
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journal_title：Experimental parasitology
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doi：10.1016/j.exppara.2006.02.018
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journal_title：Experimental parasitology
pub_type： 杂志文章
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journal_title：Experimental parasitology
pub_type： 杂志文章
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2009.05.011
更新日期：2009-09-01 00:00:00
abstract：:In the intestinal tract of fifth instars of the hematophagous reduviid bugs Rhodnius prolixus and Triatoma infestans blood ingestion induced an initial decrease of the concentration of the respective symbiotic bacteria Rhodococcus rhodnii and Nocardia sp. and then within 10 days a 15- or 18-fold increase of the total ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.2001.4653
更新日期：2002-01-01 00:00:00
abstract：:In order to investigate the factors determining the expulsion of intestinal trematodes, we have analyzed the in vivo cytokine responses at several levels and the local responses against Echinostoma caproni (Trematoda) in two host species displaying different compatibility with the parasite. The response of the high co...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2010.09.004
更新日期：2011-02-01 00:00:00
abstract：:An increased level of plasma corticosterone is one manifestation of severe environmental or physiologic stress. The stress response mediated by the hypothalamic-pituitary-adrenal axis is already known to suppress immunoglobulin production and to impair immune function, but there are few studies relating stress and pla...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2007.05.008
更新日期：2007-12-01 00:00:00
abstract：:Leishmania donovani, causative organism for visceral leishmaniasis, is responsible for considerable mortality and morbidity worldwide. Generation of drug-resistant variants continue to challenge the chemotherapy, the mainstay to fight the disease. The aim of current study was proteomic profiling of wild type (Ld-Wt) a...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2009.08.003
更新日期：2009-12-01 00:00:00
abstract：:Immunity to T. cruzi is complex, involving among other components, antibody production, CD4+ helper cells, CD8+ T cells as both regulators and effectors of immunity, and possibly, double-negative T cells. In addition, several of these components have been implicated in pathogenesis in the chronic infection. Although t...
journal_title：Experimental parasitology
pub_type： 杂志文章,评审
doi：10.1016/0014-4894(91)90013-m
更新日期：1991-07-01 00:00:00
abstract：:Giardia lamblia is a protozoan pathogen with distinct cytoskeletal structures, including median bodies and eight flagella. In this study, we examined components comprising G. lamblia flagella. Crude flagellar extracts were prepared from G. lamblia trophozoites, and analyzed by two-dimensional (2-D) gel electrophoresis...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2013.07.010
更新日期：2013-10-01 00:00:00
