Abstract:
:Culture forms of Leishmania (Leishmania) amazonensis (IFLA/BR/67/PH8) produce an extracellular enzyme that hydrolyzes sucrose molecules into their component monosaccharides. This is important because phlebotomine sand flies, the invertebrate hosts of Leishmania, ingest plant sap or aphid and coccid honeydew rich in sucrose between blood meals and Leishmania promastigotes cannot uptake sucrose. The sucrase was purified and characterized; its molecular weight, estimated by gel filtration chromatography and SDS-PAGE electrophoresis, was about 73 kDa. K(m) and V(max) measured with sucrose as substrate were respectively 4.4 mM and 6.9 mumole glucose.min-1 (mg sucrase)-1, with maximum pH activity at pH 5.5. A series of natural and p-nitrophenyl-derived substrates were assayed, characterizing the enzyme as a highly specific beta-D-fructofuranoside fructohydrolase. When 11 species of Leishmania and 7 genera of trypanosomatids were screened, only the species of the genus Trypanosoma did not produce an enzyme with saccharolytic activity. These data are in agreement with the fact that the latter vectors do not acquire sucrose or raffinose in their meals. Searching for glycolytic enzymes other than sucrase, we found an N-acetyl-beta-D-galactosaminolytic activity. This N-acetyl-galactosaminidase, here described for the first time, might have a role in peritrophic membrane disruption. The importance of sucrase and N-acetyl-beta-D-galactosaminidase in the Leishmania life cycle is discussed.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Gontijo NF,Melo MN,Riani EB,Almeida-Silva S,Mares-Guia MLdoi
10.1006/expr.1996.0055subject
Has Abstractpub_date
1996-06-01 00:00:00pages
117-24issue
1eissn
0014-4894issn
1090-2449pii
S0014-4894(96)90055-7journal_volume
83pub_type
杂志文章abstract::A systematic study of the distribution of intracellular parasites in the organs and tissues of mice acutely infected (15 days) with the CL strain of Trypanosoma cruzi was performed. Almost all tissues and organs were parasitized with different intensities, including several epithelial cell types. In addition to striat...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1996.0086
更新日期:1996-10-01 00:00:00
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abstract::To better control gastrointestinal nematode infections in humans and animals, it is important to understand the strategies used by these parasites to modulate the host immune system. In this regard, molecules released by parasites have been attributed crucially important roles in host-parasite negotiations. We charact...
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pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:2000-06-01 00:00:00
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更新日期:1988-02-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:1993-12-01 00:00:00
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更新日期:2015-03-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:1992-05-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:1995-02-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:2009-02-01 00:00:00
abstract::Giardia lamblia consist of heterogeneous isolates that can be divided into at least three groups. Differential screening of a cDNA library with isolate-specific antisera identified a gene which is expressed and found only in Group 3 isolates. This gene, GLORF-C4, is 597 bp in length and predicts a deduced protein of 1...
journal_title:Experimental parasitology
pub_type: 杂志文章
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abstract::As an approach to better understand the molecular mechanisms underlying the differential cellular response to larval Schistosoma mansoni observed in Biomphalaria glabrata, we have analyzed hemocyte membrane-associated polypeptides from inbred susceptible and resistant strains of B. glabrata. A combination of technique...
journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:1994-08-01 00:00:00