Abstract：
:Homogenates of Giardia lamblia trophozoites exhibited the following hydrolase activities: acid phosphatase (EC 3.1.3.2), proteinase (EC 3.1.4) with urea-denatured hemoglobin and N-benzoyl-DL-arginine-2-naphthylamide as substrates, deoxyribonuclease (EC 3.1.4.5), and ribonuclease (EC 2.7.7.16). beta-N-Acetylglucosaminidase (EC 3.2.1.30), beta-galactosidase (EC 3.2.1.23), beta-glucuronidase (EC 3.2.1.31), alpha-D-glucosidase (EC 3.2.1.20), beta-D-glucosidase (EC 3.2.1.21), and beta-D-xylosidase (EC 3.2.1.37) activities were below the level of detection. Differential and isopycnic centrifugation of homogenates demonstrated that giardial hydrolases were localized in a single-particle population sedimenting at 7200g for 30 min. The particles had a buoyant density in sucrose of 1.15 and exhibited latency. Latency was completely destroyed by Triton X-100 or 15 cycles of freezing and thawing. After centrifugation of Triton- or freeze-thaw-treated particle fractions, the hydrolase activities, though no longer latent, were still sedimentable suggesting tight binding to the organelle membrane. Latency was destroyed simultaneously for all hydrolases, in direct proportion to the amount of Triton added to a particle preparation or to the number of times a particle preparation was subjected to freezing and thawing. These results support the suggestion that the hydrolases of G. lamblia trophozoites are localized in a single-particle population of lysosome-like organelles.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Lindmark DGdoi
10.1016/0014-4894(88)90116-6subject
Has Abstractpub_date
1988-02-01 00:00:00pages
141-7issue
1eissn
0014-4894issn
1090-2449pii
0014-4894(88)90116-6journal_volume
65pub_type
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1995.1023
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pub_type： 杂志文章
doi：10.1016/0014-4894(86)90189-x
更新日期：1986-06-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
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journal_title：Experimental parasitology
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doi：10.1006/expr.1994.1028
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1993.1070
更新日期：1993-09-01 00:00:00
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更新日期：2001-12-01 00:00:00
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
pub_type： 杂志文章
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journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：2013-07-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(84)90055-9
更新日期：1984-02-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
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