Abstract：
:The tick Rhipicephalus microplus is an ectoparasite harmful to livestock, a vector of disease agents that affects meat and milk production. However, resistance to acaricides reflects the need for alternative tick control methods, among which vaccines have gained increasing relevance. In this scenario, monoclonal antibodies can be used to identify and characterize antigens that can be used as vaccine immunogens. Capillary tube artificial feeding of partially engorged R. microplus females with monoclonal antibodies against proteins from the gut of tick were used to test the effects of immunoglobulins in the physiology of the parasite. The results of artificial feeding showed that female ticks over 25mg and under 60 mg in weight performed better in the artificial feeding process, with a 94-168% weight increase after 24h of feeding. Results showed that artificial feeding of ticks proved to be a viable technique to study the effects of antibodies or drugs in the physiology of the parasite. One monoclonal antibody (BrBm2) induced decreased oviposition. Moreover, the antigen recognized by BrBm2 was identified as a 27-kDa protein and immunolabeled on digestive vesicles membranes of digestive cells of partially and fully engorged females.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Gonsioroski AV,Bezerra IA,Utiumi KU,Driemeier D,Farias SE,da Silva Vaz I Jr,Masuda Adoi
10.1016/j.exppara.2012.02.006subject
Has Abstractpub_date
2012-04-01 00:00:00pages
359-63issue
4eissn
0014-4894issn
1090-2449pii
S0014-4894(12)00044-6journal_volume
130pub_type
杂志文章abstract：:Acquisition of iron from lactoferrin and transferrin by a parasitic protozoon Tritrichomonas foetus has been studied in vitro. Specific, time-dependent, and saturable binding of iodinated ligands to the outer membrane of T. foetus at 4 degrees C was demonstrated for 125I-labeled lactoferrin only. About 1.7 x 10(5) bin...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1996.0068
更新日期：1996-07-01 00:00:00
abstract：:Trichinella spiralis causes a significantly higher parasite burden in rat muscle than Trichinella nativa. To assess whether the difference in infectivity is due to the early intestinal response, we analyzed gene expression changes in the rat jejunum during Trichinella infection with a whole-genome microarray. The rats...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2013.07.024
更新日期：2013-10-01 00:00:00
abstract：:Mitochondria from a rodent malarial parasite (Plasmodium berghei) were successfully purified by differential centrifugation and 22% Percoll density gradient separation. The purified mitochondria from the erythrocytic stages of the parasite had a density of 1.05 and were found to be heterogeneous by transmission electr...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1993.1070
更新日期：1993-09-01 00:00:00
abstract：:The differential effect of fox and pig bile and its corresponding low molecular weight fraction (LMW) was investigated on the in vitro invasion of MDCK-AA7 epithelial cell monolayers by Trichinella spiralis muscle larvae. Seven invasion experiments were performed and a total of 274 cell monolayers were examined. Fox a...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2010.05.013
更新日期：2010-12-01 00:00:00
abstract：:Several species of the genus Leishmania are causative agents of cutaneous leishmaniasis in Pakistan. This study aimed to determine phylogenetic placement of Leishmania species causing cutaneous leishmaniasis in Khyber Pakhtunkhwa province, Pakistan (34 Leishmania tropica, 3 Leishmania infantum), in-relation to species...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2016.05.006
更新日期：2016-08-01 00:00:00
abstract：:The role of oxidative stress in the pathogenicity of acanthamoebiasis is an important aspect of the intricate and complex host-parasite relationship. The aim of this experimental study was to determine oxidative stress through the assessment of lipid peroxidation product (LPO) levels and antioxidant defense mechanism ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2018.09.002
更新日期：2018-10-01 00:00:00
abstract：:The release of beta-glucuronidase and lysozyme from human polymorphonuclear leukocytes (PMN) engaged in phagocytosis and lysis of Trypanosoma cruzi epimastigotes was studied in the presence or absence of chagasic serum. Lysosomal enzyme release was enhanced when parasites were sensitized with serum from a chronic Chag...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(83)90069-3
更新日期：1983-10-01 00:00:00
abstract：:Trypanosoma cruzi arginine kinase is a key enzyme in cell energy management and is also involved in pH and nutritional stress response mechanisms. T. cruzi epimastigotes treated with hydrogen peroxide presented a time-dependent increase in arginine kinase expression, up to 10-fold, when compared with untreated parasit...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2006.04.004
更新日期：2006-12-01 00:00:00
abstract：:Acanthamoeba are free-living amoebae found in most environments that can cause brain and corneal infections. To infect humans, these pathogens must interact with host cells and the extracellular matrix (ECM). In order to define the mode by which amoebae recognize ECM components and process this recognition, we analyze...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2009.08.004
更新日期：2010-09-01 00:00:00
abstract：:Twenty-one Trypanosoma cruzi stocks isolated from Triatoma infestans and humans of the Chilean Andean highlands were studied at the genotypic level by schizodeme and molecular karyotype analyses, which allowed a clear distinction of the parasites from those hosts. A phenotypical characterization was performed by prote...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1995.1100
更新日期：1995-08-01 00:00:00
abstract：:Parasites of wildlife inhabiting urbanised and peri-urban environments are of interest regarding wildlife population health, and also veterinary public health in the case of parasites that can also infect humans and domestic animals. This study aimed to: identify, and estimate the prevalence of, species of Eimeria par...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2016.09.012
更新日期：2016-11-01 00:00:00
abstract：:Since we had previously demonstrated the protective role played by Toxoplasma excreted-secreted antigens, the aim of the present work was to produce monoclonal antibodies directed against these antigens in order to determine if their localization in the parasite is compatible with a mechanism of excretion or secretion...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(90)90014-4
更新日期：1990-07-01 00:00:00
abstract：:Fasciola hepatica is a digenean trematode which infects a wide variety of domestic animals and also humans. Previous studies have demonstrated that four monoclonal antibodies (Mabs) against the total extract of F. hepatica redia (named as 1E4, 6G11, 4E5 and 4G11) also recognized the excretion - secretion antigens (ES ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2016.06.010
更新日期：2016-09-01 00:00:00
abstract：:Certain model inhibitors exerted selective action against the catalytic activity of nuclear DNA topoisomerase II (TOPII) of Leishmania panamensis promastigotes. The second-generation fluoroquinolones enoxacin and ciprofloxacin exhibited extraordinarily high anti-parasite selectivity displaying 582- and 40-fold greater...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2007.02.018
更新日期：2007-08-01 00:00:00
abstract：:Snails from a B. glabrata stock, selected for nonsusceptibility to the NMRI strain of S. mansoni, exhibited unusual schistosome infections, with multiple sporocysts prominent in the head-foot (foot-sporocysts). When F1 progeny from numerous crosses between well-defined susceptible (S) and resistant (R) parental snails...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1993.1092
更新日期：1993-11-01 00:00:00
abstract：:Trypanosomatid protozoa lack consensus promoters for RNA polymerase (RNAP) II. However, the artificial insertion of the T7 promoter (P(T7)) and the tetracycline repressor into Trypanosoma brucei cell lines expressing T7RNAP allows P(T7)-driven gene expression to be tetracycline-inducible. These cell lines provide a mo...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2007.01.001
更新日期：2007-07-01 00:00:00
abstract：:The experiments described here report on the correlation between the ex vivo diffusion of different benzimidazole (BZD) anthelmintics into the cestode parasite Moniezia benedeni, and their octanol-water partition coefficients (P.C.). The characterisation of the drug diffusion process into target parasites is relevant ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/s0014-4894(03)00060-2
更新日期：2003-01-01 00:00:00
abstract：:The effect of infection by Angiostrongylus cantonensis on the activity of the enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and the concentration of total proteins, uric acid and urea in the hemolymph of Biomphalaria glabrata were investigated. The snails were dissected after 1, 2 and 3 w...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2012.03.003
更新日期：2012-06-01 00:00:00
abstract：:The prevalence of Cryptosporidium and microsporidia in feral horses, which have minimal contact with livestock and humans, is not currently known. We report the findings of a study on Cryptosporidium and microsporidia in 34 Mustangs and 50 Chincoteague ponies in the USA. Fecal samples were screened for presence of Cry...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2015.12.004
更新日期：2016-03-01 00:00:00
abstract：:Chagas' disease is caused by unicellular parasite Trypanosoma cruzi (T. cruzi). It is endemic throughout Latin America, but nowadays has become a global challenge due to tourism and migration. Non-treated infection may result in health-threatening complications and lead to death. Current medications for this infection...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2016.12.013
更新日期：2017-01-01 00:00:00
abstract：:Protein tyrosine phosphatase of regenerating liver (PRL) is a group of phosphatases that has not been broadly studied in protozoan parasites. In humans, PRLs are involved in metastatic cancer, the promotion of cell migration and invasion. PTPs have been increasingly recognized as important effectors of host-pathogen i...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2015.09.014
更新日期：2015-12-01 00:00:00
abstract：:A monoclonal antibody was generated against the large subunit of Schistosoma japonicum calpain to study the localization and possible function of the molecule in vivo. Mice were immunized with recombinant S. japonicum calpain and polyclonal antisera and a monoclonal antibody specific to schistosome calpain was obtaine...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2004.11.001
更新日期：2005-01-01 00:00:00
abstract：:Exponential weight gain equations and growth rates were determined during larval development of Brachymeria lasus reared aseptically on chemically defined media with varying amino acid and glucose levels. The rate of weight gain increased from 0 to 2% dietary glucose and mean growth rate increased from 160 to 260 micr...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(83)90028-0
更新日期：1983-06-01 00:00:00
abstract：:Leishmania is an obligate intracellular protozoan parasite that infects cells of the reticulo-endothelial system. Host defences against Leishmania include fever and oxidant production, and the parasite has developed a number of defence mechanisms to neutralize the host response. The Leishmania donovani A2 family of pr...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2012.06.001
更新日期：2012-10-01 00:00:00
abstract：:Giardia duodenalis is one of the most important intestinal parasites globally, especially in children, and in Cuba is the leading cause of chronic paediatric diarrhoea in this population. G. duodenalis is composed of eight genetic groups (or assemblages), two of which (A and B) are apparently zoonotic, occurring in bo...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2019.107814
更新日期：2020-02-01 00:00:00
abstract：:A genetically homogeneous population of Plasmodium falciparum prepared by a single erythrocyte micromanipulation technique was used to produce lines of P. falciparum resistant to mefloquine hydrochloride in vitro. Parasites were maintained in a culture medium containing gradually increased concentrations of mefloquine...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(88)90082-3
更新日期：1988-12-01 00:00:00
abstract：:There is evidence which suggests that malaria sporozoites recognize mosquito salivary glands by specific receptor-ligand interactions. We are interested in identifying the putative salivary gland receptor(s) for sporozoite invasion. We used an in vivo bioassay for sporozoite invasion of salivary glands. In this assay,...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1995.1124
更新日期：1995-11-01 00:00:00
abstract：:Optimization of desferrioxamine B (DFO) delivery for the treatment of malaria was studied in Plasmodium vinckei infected mice. DFO was administered by three different treatment regimens: (1) multiple subcutaneous injections of free DFO, (2) intraperitoneal infusion of free DFO, or (3) multiple subcutaneous injections ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1998.4282
更新日期：1998-07-01 00:00:00
abstract：:The present study was to determine the efficacies of anti-parasitic activities of synthesized silver nanoparticles (Ag NPs) using stem aqueous extract of Cissus quadrangularis against the adult of hematophagous fly, Hippobosca maculata (Diptera: Hippoboscidae), and the larvae of cattle tick, Rhipicephalus (Boophilus) ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2012.06.009
更新日期：2012-10-01 00:00:00
abstract：:Chemotherapy of food-borne trematodes relies on two drugs, praziquantel and tricabendazole, and there is growing interest in finding alternative therapies. Plant oil extracts have long been used in traditional Chinese medicine as sources of bioactive compounds with antiparasitic activity. Species of the genus Echinost...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2017.01.003
更新日期：2017-03-01 00:00:00
