Abstract：
:The release of beta-glucuronidase and lysozyme from human polymorphonuclear leukocytes (PMN) engaged in phagocytosis and lysis of Trypanosoma cruzi epimastigotes was studied in the presence or absence of chagasic serum. Lysosomal enzyme release was enhanced when parasites were sensitized with serum from a chronic Chagas' patient, increased up to 3 hr of incubation at 28 C, and depended on the PMN:parasite ratio. The release of lysosomal enzymes was determined by the presence of 2 mM cyanide, 2 microM azide, 3 mM amobarbital, and 1 mM phenylbutazone. These drugs inhibited the killing of sensitized T. cruzi by interfering with the oxidative microbicidal mechanisms of PMN without affecting the uptake of the parasites. Lysosomal enzyme release occurred in the presence of cyanide and azide, indicating that in these cases the enzymatic release was unrelated to the killing of the parasites. Amobarbital and phenylbutazone, which stabilize PMN membranes, inhibited the release of beta-glucuronidase and lysozyme by PMN. The addition of 10 micrograms/ml of cytochalasin B inhibited the phagocytosis and killing of sensitized T. cruzi by PMN but increased the enzymatic release by effector cells. Since cytochalasin B did not affect the close contact between PMN and parasites, it appears that the enzymes released to the extracellular milieu were not toxic to noningested parasites. Furthermore, the lysosomal enzymes did not lyse bystander unsensitized parasites. Therefore, the release of lysosomal enzymes during the interaction of T. cruzi epimastigotes and PMN seems to be related to the triggering event of the phagocytic process and does not bear a cause-effect relationship with parasite death.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
de Titto EH,Cardoni RLdoi
10.1016/0014-4894(83)90069-3subject
Has Abstractpub_date
1983-10-01 00:00:00pages
247-54issue
2eissn
0014-4894issn
1090-2449pii
0014-4894(83)90069-3journal_volume
56pub_type
杂志文章abstract：:Toxoplasma gondii is responsible for fetopathy in farm animals and humans and severe disease in immunocompromised individuals (i.e. AIDS patients). Effective vaccines, inducing protective and long-lasting immunity to this global parasite, are still desired. In the work, we evaluated the immunogenic and immunoprotectiv...
journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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doi：10.1006/expr.1994.1053
更新日期：1994-08-01 00:00:00
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更新日期：2018-04-01 00:00:00
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journal_title：Experimental parasitology
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更新日期：1989-11-01 00:00:00
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journal_title：Experimental parasitology
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更新日期：1989-10-01 00:00:00
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pub_type： 杂志文章
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更新日期：2020-08-01 00:00:00
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journal_title：Experimental parasitology
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更新日期：1997-03-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：1984-06-01 00:00:00
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更新日期：2013-05-01 00:00:00
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更新日期：2009-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1006/expr.1993.1064
更新日期：1993-08-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2009.11.019
更新日期：2010-09-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1996.0068
更新日期：1996-07-01 00:00:00
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pub_type： 杂志文章
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更新日期：2014-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0014-4894(88)90012-4
更新日期：1988-10-01 00:00:00
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pub_type： 杂志文章
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更新日期：1986-08-01 00:00:00
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更新日期：2016-11-01 00:00:00
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更新日期：1983-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：2007-09-01 00:00:00
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pub_type： 杂志文章
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journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：1992-08-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：2013-01-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：2013-06-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：2008-07-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：2013-05-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：2019-02-01 00:00:00
abstract：:The effects of azadirachtin and ecdysone on the Trypanosoma cruzi population in the Rhodnius prolixus gut were investigated. T. cruzi were rarely found in the gut compartments of azadirachtin-treated larvae. High parasite numbers were observed in the stomach of the control and ecdysone groups until 10 days after treat...
journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：2012-07-01 00:00:00
abstract：:Indirect immunocytochemistry, in conjunction with confocal scanning laser microscopy and electron-microscopic immunogold labeling, has been used to localize neuropeptide and 5-hydroxytryptamine (5-HT) immunoreactivities (IRs) in the plerocercoid (scolex and surrounding blastocyst) of the trypanorhynch tapeworm, Grillo...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1994.1103
更新日期：1994-11-01 00:00:00
