Abstract:
:Two recombinant barley cystatins, HvCPI5 and HvCPI6, have been tested in vitro against promastigotes and intracellular amastigotes of Leishmania infantum in the J774 monocytic cell line. Toxicity of cystatins for J774 cells was also determined. In addition, a comparison between direct counts of intracellular amastigotes and quantitation of burden by Q-PCR was carried out. Low concentrations (2 microM) from both cystatins were unable to inhibit promastigote replication. HvCPI5 was toxic for mammalian cells; 0.1 microM reduced by more than 50% the cell viability. On the contrary, HvCPI6 did not exhibit any toxicity for J774 cells up to 6 microM and inhibited the intracellular amastigote multiplication. Dose-response analysis showed that 4.8 microM HvCPI6 reduced by >90% the intracellular parasite load and had an approximate IC(50) value of 1.5 microM. Comparable results were obtained by direct counting of intracellular amastigotes and Q-PCR. Results point towards the direct inhibition of amastigote multiplication by HvCPI6 and the interest of this recombinant cystatin in the chemotherapy of leishmaniasis.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Ordóñez-Gutiérrez L,Martínez M,Rubio-Somoza I,Díaz I,Mendez S,Alunda JMdoi
10.1016/j.exppara.2009.08.015subject
Has Abstractpub_date
2009-12-01 00:00:00pages
341-6issue
4eissn
0014-4894issn
1090-2449pii
S0014-4894(09)00252-5journal_volume
123pub_type
杂志文章abstract::Aspartic proteases are important virulence factors for pathogens and are recognized as attractive drug targets. Seven aspartic proteases (ASPs) have been identified in Toxoplasma gondii genome. Bioinformatics and phylogenetic analyses regroup them into five monophyletic groups. Among them, TgASP1, a coccidian specific...
journal_title:Experimental parasitology
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2008.04.013
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
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doi:10.1016/j.exppara.2009.01.001
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2008.04.014
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journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
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abstract::Benznidazole is the first-line drug used in treating Chagas disease, which is caused by the parasite Trypanosoma cruzi (T. cruzi). However, benznidazole has limited efficacy and several adverse reactions. Pentamidine is an antiprotozoal drug used in the treatment of leishmaniasis and African trypanosomiasis. In T. cru...
journal_title:Experimental parasitology
pub_type: 杂志文章
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