Abstract：
:Like most intracellular pathogens, the apicomplexan parasites Besnoitia besnoiti, Toxoplasma gondii and Neospora caninum scavenge metabolites from their host cells. Recruitment of the Golgi complex to the vicinity of the parasitophorous vacuole (PV) is likely to aid in this process. In this work, we comparatively assessed B. besnoiti, T. gondii and N. caninum infected human retinal pigmented epithelial (hTERT-RPE-1) cells at 24 h post-infection and used antibodies to confirm Golgi ribbon compaction in B. besnoiti, and Golgi ribbon dispersion in T. gondii, while no alteration in Golgi morphology was seen in N. caninum infected cells. In either case, the Golgi stacks of infected cells contained both cis- (GM130) and trans- (TGN46) Golgi proteins. The localization of Rab9A, an important regulator of endosomal trafficking, was also studied. GFP-tagged Rab9A was recruited to the vicinity of the PV of all three parasites. Toxoplasma-infected cells exhibited increased expression of Rab9A in comparison to non-infected cells. However, Rab9A expression levels remained unaltered upon infection with N. caninum and B. besnoiti tachyzoites. In contrast to Rab9A, a GFP-tagged dominant negative mutant form of Rab9A (Rab9A DN), was not recruited to the PV, and the expression of Rab9A DN did not affect host cell invasion nor replication by all three parasites. Thus, B. besnoiti, T. gondii and N. caninum show similarities but also differences in how they affect constituents of the endosomal/secretory pathways.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Cardoso R,Wang J,Müller J,Rupp S,Leitão A,Hemphill Adoi
10.1016/j.exppara.2018.02.008subject
Has Abstractpub_date
2018-04-01 00:00:00pages
75-85eissn
0014-4894issn
1090-2449pii
S0014-4894(17)30469-1journal_volume
187pub_type
杂志文章abstract：:Matrix metalloproteinases (MMPs) constitute a large family of Zn(2+) and Ca(2+) dependent endopeptidases implicated in tissue remodeling and chronic inflammation. MMPs also play key roles in the activation of growth factors, chemokines and cytokines produced by many cell types, including lymphocytes, granulocytes, and...
journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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doi：10.1016/j.exppara.2018.03.002
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2009.05.011
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journal_title：Experimental parasitology
pub_type： 杂志文章
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journal_title：Experimental parasitology
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abstract：:S(2) complex has been reported to have a direct antileishmanial effect. The possibility that the direct antileishmanial effect may be due to inhibition of key enzymes involved in glucose metabolism and/ or enzymes associated with virulence was investigated. Cell pellets were prepared from cultures of both axenic amast...
journal_title：Experimental parasitology
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doi：10.1016/j.exppara.2005.09.006
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journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：2019-09-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1997.4159
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2004.03.014
更新日期：2004-05-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2010.08.021
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abstract：:The existence of cholinergic receptors in the immune system cells is well documented. This study aimed to evaluate the acetylcholinesterase activity (AChE) in lymphocytes from rats infected with Trypanosoma evansi in acute and chronic phase disease. Twenty animals were infected with 10(6) trypomastigotes forms each an...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2010.10.017
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journal_title：Experimental parasitology
pub_type： 杂志文章
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
pub_type： 杂志文章,评审
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
pub_type： 杂志文章
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journal_title：Experimental parasitology
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abstract：:Chagas disease is a tropical and systemic disease caused by the parasite Trypanosoma cruzi. This parasite has been divided into six Discrete Typing Units (DTU's) due to its high genetic diversity. T. cruzi I (TcI) is the most prevalent DTU in Colombia and recently associated to cardiomyopathies. The aim of this study ...
journal_title：Experimental parasitology
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doi：10.1016/j.exppara.2012.09.017
更新日期：2012-12-01 00:00:00
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/s0014-4894(02)00135-2
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abstract：:It is well known that Anopheles dirus is naturally refractory to rodent malaria parasite, Plasmodium yoelii, but the mechanism is still largely unknown. Here, we found that some P. yoelii taken into An. dirus could develop into oocysts, but oocysts were partially melanized at 7 days and completely melanized at 15 days...
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abstract：:Murine infection with Taenia crassiceps cysticerci is used as an experimental model for human and animal cysticercosis. In this infection parasites can be found associated with an inflammatory infiltrate enriched with macrophages. Experimental evidence exists supporting a role for either NO-producing classically activ...
journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：2013-11-01 00:00:00
