Abstract：
:Isocitrate lyase (EC 4.1.3.1) from mixed larval populations of Caenorhabditis elegans was stabilized in crude extracts by centrifugation over a 0.2-0.6 M sucrose gradient for 2.5 hr in a vertical rotor (VTi 50) at 210,000g. The peak fractions from this sucrose gradient showed a half-life of 33 hr at 30 C and 225 hr at 4 C in contrast to 2.5 and 52 hr, respectively, for the crude extract. A purification scheme involving (NH4)2SO4 precipitation and chromatography on Sepharose 6B and diethylaminoethyl-cellulose yielded isocitrate lyase that gave one band after electrophoresis in a sodium dodecyl sulfate-gel polymerized from 12% acrylamide. The purified enzyme with a molecular weight of 250,000 and subunit molecular weight of 61,600, had a specific activity of 2 mumoles glyoxylate formed min-1 mg protein-1, and was obtained in a 4% yield. Isocitrate lyase from C. elegans lost 80-85% of its activity in the precipitation by 33-55% (NH4)2SO4, but this step appeared to be necessary for purification to homogeneity. The use of fast protein liquid chromatography appeared to be promising in that it provided an enzyme preparation that was about 50% pure with a specific activity as high as 3 mumoles glyoxylate formed min-1 mg protein-1. Poly(A+)RNA was isolated from C. elegans and translated in wheat germ cell-free system. Analysis on a 10% sodium dodecyl sulfate-polyacrylamide gel showed varied translation products including one or more 60,000-Da polypeptides.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Jameel S,McFadden BAdoi
10.1016/0014-4894(85)90089-xsubject
Has Abstractpub_date
1985-06-01 00:00:00pages
337-46issue
3eissn
0014-4894issn
1090-2449journal_volume
59pub_type
杂志文章abstract：:The expression and activity of topoisomerase I (PfTopoI) has been examined during the intraerythrocytic stages of the Plasmodium falciparum life cycle. The promoter is inactive during the early ring stage and becomes active only during the later trophozoite and schizont stages. The PfTOP1 transcript starts to accumula...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1998.4362
更新日期：1999-02-01 00:00:00
abstract：:Two species of macaques, including two Macaca fascicularis from the Philippines, two M. fascicularis from Mauritius, and one Macaca mulatta, were experimentally infected with blood stages of Plasmodium coatneyi and followed during their clinical, parasitological, biological, and immunological evolution. Plasma cytokin...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1999.4342
更新日期：1999-01-01 00:00:00
abstract：:Early diagnosis and determining the infective stage are critical for effective therapy of toxoplasmosis. Owing to the progress in biotechnology, commonly used native, non-standardized diagnostic antigens should be replaced by genetically engineered antigens. The recombinant proteins are also promising components of su...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2015.01.006
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2007.01.019
更新日期：2007-08-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2013.03.025
更新日期：2013-07-01 00:00:00
abstract：:Baccharis dracunculifolia DC (common name "alecrim-do-campo" in Brazil) is a plant with widespread distribution in South America that is the botanical origin of green propolis. The aim of this study was to evaluate the chemical composition and acaricidal activity of the essential oil of B. dracunculifolia and its cons...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2014.10.011
更新日期：2015-01-01 00:00:00
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journal_title：Experimental parasitology
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doi：10.1016/j.exppara.2013.01.021
更新日期：2013-05-01 00:00:00
abstract：:Acanthamoeba is a free-living amoeba genus that causes several diseases namely, amoebic keratitis which is a painful sight threatening eyes disease. Its treatment is difficult and the exploration for new drugs is very important. The main objective of the present study was to evaluate the chemical composition of the Es...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2017.10.011
更新日期：2017-12-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1996.0119
更新日期：1996-12-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1993.1070
更新日期：1993-09-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1995.1057
更新日期：1995-05-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1995.1023
更新日期：1995-03-01 00:00:00
abstract：:Eosinophilia in blood and tissues has been strongly associated with helminth infections for over a century. In vivo depletion of IL-5, a cytokine crucially involved in eosinophilopoiesis with an antibody or through genetic manipulation, reproducibly abrogates helminth-induced eosinophilia, but renders mice permissive ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2003.09.001
更新日期：2003-10-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1998.4260
更新日期：1998-05-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2012.03.013
更新日期：2012-06-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2019.01.010
更新日期：2019-03-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2016.07.004
更新日期：2016-10-01 00:00:00
abstract：:Toxocara canis is a dog helminth which causes visceral larva migrans (VLM) when infecting humans as a larva. The infection is demonstrated by detecting IgG antibodies against excretory-secretory larval antigens (ESLA) in serum by ELISA. The production of ESLA involves the collection of adult worms from dog puppy stool...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2008.03.006
更新日期：2008-07-01 00:00:00
abstract：:High-throughput drug screening methods against the intracellular stage of Leishmania have been facilitated by the development of in vitro models of infection. The use of cell lines rather than primary cells facilitates these methods. Peripheral blood mononuclear cell (PBMC) derived macrophages and THP-1 cells were inf...
journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：2010-12-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2012.09.017
更新日期：2012-12-01 00:00:00
abstract：:Lipophosphoglycan (LPG) is structurally characterized by a series of phosphoglycan repeat units. Cellular LPG, isolated from promastigotes, has a very similar structure to culture supernatant LPG, but differs in the average number of phosphorylated oligosaccharide repeat units and in glycan composition. Comparison of ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2009.09.017
更新日期：2010-02-01 00:00:00
abstract：:The biochemical and morphological events which accompany lysis of Trypanosoma brucei by normal human serum have been described. The prelytic events include loss of infectivity and rapid cation shifts across the cell membrane. This is followed by cell swelling, fraying of the surface coat of the cell, loss of intracell...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(84)90023-7
更新日期：1984-08-01 00:00:00
abstract：:Molecular characterizations of Cryptosporidium spp. in ruminants reared under traditional animal management systems are scarce and studies conducted thus far have revealed largely an absence of the pathogenic and zoonotic species Cryptosporidium parvum in pre-weaned animals. In this study, we examined Cryptosporidium ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2016.06.014
更新日期：2016-11-01 00:00:00
abstract：:Nitric oxide (NO) has diverse biological functions. Numerous studies have documented NO's biosynthetic pathway in a wide variety of organisms. Little is known, however, about NO production in intraerythrocytic Plasmodium falciparum. Using diaminorhodamine-4-methyl acetoxymethylester (DAR-4M AM), a fluorescent indicato...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2008.04.014
更新日期：2008-09-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2014.04.003
更新日期：2014-07-01 00:00:00
abstract：:Protein tyrosine phosphorylation (PY) is a well-known signalling mechanism which is also involved in host-parasite interactions. Despite its transcendence, PY has been poorly studied in parasitic helminths. The aim of this study is to examine the effect of bile salts on the PY pattern in parasitic trematodes. Two dist...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2004.01.006
更新日期：2004-01-01 00:00:00
abstract：:Considering that the treatment for toxoplasmosis is based on drugs that show limited efficacy due to their substantial side effects, the purpose of the present study was to evaluate the effects of Artemisia annua on in vitro and in vivo Toxoplasma gondii infection. A. annua infusion was prepared from dried herb and te...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2009.04.010
更新日期：2009-07-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(92)90151-y
更新日期：1992-05-01 00:00:00
abstract：:This report describes a molecular method for determining in a first step the generic composition of a nematode community and in a second step, the resistance of each species to benzimidazole (BZ). We first established a polymerase chain reaction (PCR) linked to a restriction fragment length polymorphism strategy using...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.2000.4542
更新日期：2000-08-01 00:00:00
abstract：:To analyze whether nitric oxide may be involved in the pathogenesis of the mouse cerebral malaria (CM), nitrate and nitrite were first measured in urines of Plasmodium species infected mice. The CM-susceptible CBA/J mice were infected with either Plasmodium berghei or Plasmodium chabaudi, and the CM-resistant BALB/c m...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1993.1066
更新日期：1993-08-01 00:00:00
