解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Citrus fruit has a unique structure with soft leathery peel and pulp containing vascular bundles and several segments with many juice sacs. The function and morphology of each fruit tissue are different. Therefore, analysis at the organ-wide or mixed-tissue level inevitably obscures many tissue-specific phenomena. Hig...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13549
更新日期:2021-01-20 00:00:00
abstract::Interspecific genomic variation can provide a genetic basis for local adaptation and domestication. A series of studies have presented its role of interspecific haplotypes and introgressions in adaptive traits, but few studies have addressed their role in improving agronomic character. Two allotetraploid Gossypium spe...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13547
更新日期:2021-01-14 00:00:00
abstract::Low grain moisture at harvest is crucial for safe production, transport, and storage, but the genetic architecture of this trait in maize (Zea mays) remains elusive. Here, we measured the dynamic changes in grain moisture content in an association mapping panel of 513 diverse maize inbred lines at five successive stag...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13541
更新日期:2021-01-01 00:00:00
abstract::Increased use of nitrogen fertilizers has deleterious impact on the environment. Increase in yield potential at low nitrogen supply is regarded as a cereal breeding goal for future agricultural sustainability. Although natural variations of nitrogen transporters have been investigated, key genes associated with assimi...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13450
更新日期:2021-01-01 00:00:00
abstract::Salt stress has detrimental effects on crop growth and yield, and the area of salt-affected land is increasing. Soybean is a major source of vegetable protein, oil and feed, but considered as a salt-sensitive crop. Cultivated soybean (Glycine max) is domesticated from wild soybean (G. soja) but lost considerable amoun...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13536
更新日期:2020-12-25 00:00:00
abstract::Glyphosate is a popular, systemic, broad-spectrum herbicide used in modern agriculture. Being a structural analog of phosphoenolpyruvate (PEP), it inhibits 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) which is responsible for the biosynthesis of aromatic amino acids and various aromatic secondary metabolites. T...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13428
更新日期:2020-12-01 00:00:00
abstract::The Chinese jujube (Ziziphus jujuba Mill.), a member of the Rhamnaceae family, is an important perennial fruit tree crop of substantial economic, ecological and nutritional value, and is also used as a traditional herbal medicine. Here, we report the resequencing of 493 jujube accessions, including 202 wild and 291 cu...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13480
更新日期:2020-09-18 00:00:00
abstract::In the last 20 years, stem rust caused by the fungus Puccinia graminis f. sp. tritici (Pgt), has re-emerged as a major threat to wheat and barley production in Africa and Europe. In contrast to wheat with 60 designated stem rust (Sr) resistance genes, barley's genetic variation for stem rust resistance is very narrow ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13460
更新日期:2020-08-03 00:00:00
abstract::Soybean cyst nematode (SCN, Heterodera glycines) is the most devastating pest affecting soybean production worldwide. SCN resistance requires both the GmSHMT08 and the GmSNAP18 in 'Peking'-type resistance. Here, we describe the molecular interaction between GmSHMT08 and GmSNAP18, which is potentiated by a pathogenesis...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13343
更新日期:2020-08-01 00:00:00
abstract::The plant immune system involves detection of pathogens via both cell-surface and intracellular receptors. Both receptor classes can induce transcriptional reprogramming that elevates disease resistance. To assess differential gene expression during plant immunity, we developed and deployed quantitative sequence captu...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13327
更新日期:2020-07-01 00:00:00
abstract::The plant pathogen, Pseudomonas syringae (Ps), together with related Ps species, infects and attacks a wide range of agronomically important crops, including tomato, kiwifruit, pepper, olive and soybean, causing economic losses. Currently, chemicals and introduced resistance genes are used to protect plants against th...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13294
更新日期:2020-05-01 00:00:00
abstract::Anthocyanins have crucial biological functions and affect quality of horticultural produce. Anthocyanins accumulate in ripe peach fruit; differential accumulation is observed in deep coloured cultivar 'Hujingmilu' and lightly pigmented cultivar 'Yulu'. The difference was not fully explained by accumulation of total fl...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13291
更新日期:2020-05-01 00:00:00
abstract::Methods based on single nucleotide polymorphism (SNP), copy number variation (CNV) and presence/absence variation (PAV) discovery provide a valuable resource to study gene structure and evolution. However, as a result of these structural variations, a single reference genome is unable to cover the entire gene content ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13262
更新日期:2020-04-01 00:00:00
abstract::The subspecies fastigiata of cultivated groundnut lost fresh seed dormancy (FSD) during domestication and human-made selection. Groundnut varieties lacking FSD experience precocious seed germination during harvest imposing severe losses. Development of easy-to-use genetic markers enables early-generation selection in ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13266
更新日期:2020-04-01 00:00:00
abstract::For future food security, it is important that wheat, one of the most widely consumed crops in the world, can survive the threat of abiotic and biotic stresses. New genetic variation is currently being introduced into wheat through introgressions from its wild relatives. For trait discovery, it is necessary that each ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13241
更新日期:2020-03-01 00:00:00
abstract::China is the origin and evolutionary centre of Oriental pears. Pyrus betuleafolia is a wild species native to China and distributed in the northern region, and it is widely used as rootstock. Here, we report the de novo assembly of the genome of P. betuleafolia-Shanxi Duli using an integrated strategy that combines Pa...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13226
更新日期:2020-02-01 00:00:00
abstract::The rice root system is important for growth. The crosstalk between auxin and cytokinin mediates root initiation and elongation. However, it remains unclear how the transcriptional network upstream of the auxin and cytokinin signalling pathways determines root development. Here, we observed that the knockdown of OsNAC...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13209
更新日期:2020-02-01 00:00:00
abstract::Grain size and plant architecture are critical factors determining crop productivity. Here, we performed gene editing of the MIR396 gene family in rice and found that MIR396e and MIR396f are two important regulators of grain size and plant architecture. mir396ef mutations can increase grain yield by increasing grain s...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13214
更新日期:2020-02-01 00:00:00
abstract::Tomato (Solanum lycopersicum) fruit ripening is regulated co-operatively by the action of ethylene and a hierarchy of transcription factors, including RIPENING INHIBITOR (RIN) and NON-RIPENING (NOR). Mutations in these two genes have been adopted commercially to delay ripening, and accompanying textural deterioration,...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13176
更新日期:2020-01-01 00:00:00
abstract::Clustered regularly interspaced short palindromic repeat (CRISPR) and Cas9-associated protein systems provide a powerful genetic manipulation tool that can drive plant research forward. Nuclease-dead Cas9 (dCas9) is an enzymatically inactive mutant of Cas9 in which its endonuclease activity is non-functional. The appl...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/pbi.13232
更新日期:2020-01-01 00:00:00
abstract::Bacterial wilt, caused by Ralstonia solanacearum, is a devastating disease affecting over 350 plant species. A few peanut cultivars were found to possess stable and durable bacterial wilt resistance (BWR). Genomics-assisted breeding can accelerate the process of developing resistant cultivars by using diagnostic marke...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13153
更新日期:2019-12-01 00:00:00
abstract::Recently, CRISPR-Cas12a (Cpf1) from Prevotella and Francisella was engineered to modify plant genomes. In this report, we employed CRISPR-LbCas12a (LbCpf1), which is derived from Lachnospiraceae bacterium ND2006, to edit a citrus genome for the first time. First, LbCas12a was used to modify the CsPDS gene successfully...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13109
更新日期:2019-10-01 00:00:00
abstract::The development and adoption of hybrid seed technology have led to dramatic increases in agricultural productivity. However, it has been a challenge to develop a commercially viable platform for the production of hybrid wheat (Triticum aestivum) seed due to wheat's strong inbreeding habit. Recently, a novel platform f...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13106
更新日期:2019-10-01 00:00:00
abstract::Porcine circovirus type 2 (PCV-2) is the main causative agent associated with a group of diseases collectively known as porcine circovirus-associated disease (PCAD). There is a significant economic strain on the global swine industry due to PCAD and the production of commercial PCV-2 vaccines is expensive. Plant expre...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13097
更新日期:2019-09-01 00:00:00
abstract::Soybean cyst nematode (SCN) is the most devastating plant-parasitic nematode. Most commercial soybean varieties with SCN resistance are derived from PI88788. Resistance derived from PI88788 is breaking down due to narrow genetic background and SCN population shift. PI88788 requires mainly the rhg1-b locus, while 'Peki...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13086
更新日期:2019-08-01 00:00:00
abstract::Glycolate is produced in autotrophic cells under high temperatures and Ci -limitation via oxygenation of ribulose-1,5-bisphosphate. In unicellular algae, glycolate is lost via excretion or metabolized via the C2 cycle by consuming reductants, ATP and CO2 emission (photorespiration). Therefore, photorespiration is an i...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13078
更新日期:2019-08-01 00:00:00
abstract::Co-expression of protease inhibitors like the tomato cystatin SlCYS8 is useful to increase recombinant protein production in plants, but key proteases involved in protein proteolysis are still unknown. Here, we performed activity-based protein profiling to identify proteases that are inhibited by SlCYS8 in agroinfiltr...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13092
更新日期:2019-08-01 00:00:00
abstract::Dengue fever is a mosquito (Aedes aegypti) -transmitted viral disease that is endemic in more than 125 countries around the world. There are four serotypes of the dengue virus (DENV 1-4) and a safe and effective dengue vaccine must provide protection against all four serotypes. To date, the first vaccine, Dengvaxia (C...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13065
更新日期:2019-07-01 00:00:00
abstract::Considered responsible for one million deaths in Ireland and widespread famine in the European continent during the 1840s, late blight, caused by Phytophthora infestans, remains the most devastating disease of potato (Solanum tuberosum L.) with about 15%-30% annual yield loss in sub-Saharan Africa, affecting mainly sm...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13042
更新日期:2019-06-01 00:00:00
abstract::Steviol glycosides (SGs) are extracted from Stevia leaves for use as a natural sweetener. Among SGs, stevioside is most abundant in leaf extracts followed by rebaudioside A (Reb A). However, Reb A is of particular interest because of its sweeter and more pleasant taste compared to stevioside. Therefore, the developmen...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13035
更新日期:2019-06-01 00:00:00
abstract::Triterpenes are thirty-carbon compounds derived from the universal five-carbon prenyl precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Normally, triterpenes are synthesized via the mevalonate (MVA) pathway operating in the cytoplasm of eukaryotes where DMAPP is condensed with two IPPs to...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12983
更新日期:2019-02-01 00:00:00
abstract::Sugarcane (Saccharum spp.) is a highly energy-efficient crop primarily for sugar and bio-ethanol production. Sugarcane genetics and cultivar improvement have been extremely challenging largely due to its complex genomes with high polyploidy levels. In this study, we deeply sequenced the coding regions of 307 sugarcane...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12992
更新日期:2019-02-01 00:00:00
abstract::Following the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing (dRenSeq) enables the high-confidence i...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12997
更新日期:2019-02-01 00:00:00
abstract::Plants offer fast, flexible and easily scalable alternative platforms for the production of pharmaceutical proteins, but differences between plant and mammalian N-linked glycans, including the presence of β-1,2-xylose and core α-1,3-fucose residues in plants, can affect the activity, potency and immunogenicity of plan...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12981
更新日期:2019-02-01 00:00:00
abstract::Whole-genome sequencing-based bulked segregant analysis (BSA) for mapping quantitative trait loci (QTL) provides an efficient alternative approach to conventional QTL analysis as it significantly reduces the scale and cost of analysis with comparable power to QTL detection using full mapping population. We tested the ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12964
更新日期:2019-01-01 00:00:00
abstract::Receptor-like kinases (RLKs) are important components of plant innate immunity. Although recent studies have revealed that the RLK suppressor of BIR1-1 (SOBIR1) can interact with multiple receptor-like proteins and is required for resistance against fungal pathogens, how the signal is transduced and triggers immune re...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12954
更新日期:2019-01-01 00:00:00
abstract::The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12952
更新日期:2019-01-01 00:00:00
abstract::Arsenic (As) is a poisonous element that causes severe skin lesions and cancer in humans. Rice (Oryza sativa L.) is a major dietary source of As in humans who consume this cereal as a staple food. We hypothesized that increasing As vacuolar sequestration would inhibit its translocation into the grain and reduce the am...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12905
更新日期:2018-10-01 00:00:00
abstract::N-glycosylation is critical for recombinant glycoprotein production as it influences the heterogeneity of products and affects their biological function. In most eukaryotes, the oligosaccharyltransferase is the central-protein complex facilitating the N-glycosylation of proteins in the lumen of the endoplasmic reticul...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12906
更新日期:2018-10-01 00:00:00
abstract::Genetically engineered (GE) rice lines expressing Lepidoptera-active insecticidal cry genes from the bacterium Bacillus thuringiensis (Bt) have been developed in China. Field surveys indicated that Bt rice harbours fewer rice planthoppers than non-Bt rice although planthoppers are not sensitive to the produced Bt Cry ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12911
更新日期:2018-10-01 00:00:00