An oligosaccharyltransferase from Leishmania major increases the N-glycan occupancy on recombinant glycoproteins produced in Nicotiana benthamiana.

abstract：:An important goal in biotechnological research is to improve the yield of crop plants. Here, we genetically modified simultaneously source and sink capacities in potato (Solanum tuberosum cv. Desirée) plants to improve starch yield. Source capacity was increased by mesophyll-specific overexpression of a pyrophosphatas...

journal_title：Plant biotechnology journal

authors： Jonik C,Sonnewald U,Hajirezaei MR,Flügge UI,Ludewig F

更新日期：2012-12-01 00:00:00

abstract：:Recently, CRISPR-Cas12a (Cpf1) from Prevotella and Francisella was engineered to modify plant genomes. In this report, we employed CRISPR-LbCas12a (LbCpf1), which is derived from Lachnospiraceae bacterium ND2006, to edit a citrus genome for the first time. First, LbCas12a was used to modify the CsPDS gene successfully...

journal_title：Plant biotechnology journal

authors： Jia H,Orbović V,Wang N

更新日期：2019-10-01 00:00:00

abstract：:For future food security, it is important that wheat, one of the most widely consumed crops in the world, can survive the threat of abiotic and biotic stresses. New genetic variation is currently being introduced into wheat through introgressions from its wild relatives. For trait discovery, it is necessary that each ...

journal_title：Plant biotechnology journal

authors： Grewal S,Hubbart-Edwards S,Yang C,Devi U,Baker L,Heath J,Ashling S,Scholefield D,Howells C,Yarde J,Isaac P,King IP,King J

更新日期：2020-03-01 00:00:00

abstract：:Although much explored, oral tolerance for treatment of autoimmune diseases still awaits the establishment of novel and effective vectors. We investigated whether the tolerogenic CTA1(R7K)-COL-DD fusion protein can be expressed in edible plants, to induce oral tolerance and protect against arthritis. The fusion protei...

journal_title：Plant biotechnology journal

authors： Hansson C,Schön K,Kalbina I,Strid Å,Andersson S,Bokarewa MI,Lycke NY

更新日期：2016-04-01 00:00:00

abstract：:Transgenic rice accumulating the modified major Japanese cedar pollen allergens, Cryptomeria japonica 1 (Cry j 1) and Cryptomeria japonica 2 (Cry j 2), which were deconstructed by fragmentation and shuffling, respectively, in the edible part of the seed was generated by transformation of a good-tasting rice variety, '...

journal_title：Plant biotechnology journal

authors： Wakasa Y,Takagi H,Hirose S,Yang L,Saeki M,Nishimura T,Kaminuma O,Hiroi T,Takaiwa F

更新日期：2013-01-01 00:00:00

abstract：:Porcine circovirus type 2 (PCV-2) is the main causative agent associated with a group of diseases collectively known as porcine circovirus-associated disease (PCAD). There is a significant economic strain on the global swine industry due to PCAD and the production of commercial PCV-2 vaccines is expensive. Plant expre...

journal_title：Plant biotechnology journal

authors： Gunter CJ,Regnard GL,Rybicki EP,Hitzeroth II

更新日期：2019-09-01 00:00:00

abstract：:Traditional breeding methods are hindered in bananas due to the fact that major cultivars are sterile, parthenocarpic, triploid and thus clonally propagated. This has resulted in a narrow genetic base and limited resilience to biotic and abiotic stresses. Mutagenesis of in vitro propagated bananas is one method to int...

journal_title：Plant biotechnology journal

authors： Datta S,Jankowicz-Cieslak J,Nielen S,Ingelbrecht I,Till BJ

更新日期：2018-02-24 00:00:00

abstract：:One goal of modern agriculture is the improvement of plant drought tolerance and water-use efficiency (WUE). Although stomatal density has been linked to WUE, the causal molecular mechanisms and engineered alternations of this relationship are not yet fully understood. Moreover, YODA (YDA), which is a MAPKK kinase gen...

journal_title：Plant biotechnology journal

authors： Meng LS,Yao SQ

更新日期：2015-09-01 00:00:00

abstract：:The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid...

journal_title：Plant biotechnology journal

authors： Tomlinson L,Yang Y,Emenecker R,Smoker M,Taylor J,Perkins S,Smith J,MacLean D,Olszewski NE,Jones JDG

更新日期：2019-01-01 00:00:00

abstract：:Multivitamin corn is a novel genetically engineered variety that simultaneously produces high levels of β-carotene, ascorbate and folate, and therefore has the potential to address simultaneously multiple micronutrient deficiencies caused by the lack of vitamins A, B9 and C in developing country populations. As part o...

journal_title：Plant biotechnology journal

authors： Arjó G,Capell T,Matias-Guiu X,Zhu C,Christou P,Piñol C

更新日期：2012-12-01 00:00:00

abstract：:Transgenic Bt rice expressing the insecticidal proteins derived from Bacillus thuringiensis Berliner (Bt) has been developed since 1989. Their ecological risks towards non-target organisms have been investigated; however, these studies were conducted individually, yielding uncertainty regarding potential agroecologica...

journal_title：Plant biotechnology journal

authors： Dang C,Lu Z,Wang L,Chang X,Wang F,Yao H,Peng Y,Stanley D,Ye G

更新日期：2017-08-01 00:00:00

abstract：:The root system is fundamental for plant development, is crucial for overall plant growth and is recently being recognized as the key for future crop productivity improvement. A major determinant of root system architecture is the initiation of lateral roots. While knowledge of the genetic and molecular mechanisms reg...

journal_title：Plant biotechnology journal

authors： Jansen L,Hollunder J,Roberts I,Forestan C,Fonteyne P,Van Quickenborne C,Zhen RG,McKersie B,Parizot B,Beeckman T

更新日期：2013-12-01 00:00:00

abstract：:MicroRNAs (miRNAs) are a class of regulatory small RNAs (sRNAs) that down-regulate target genes through mRNA cleavage or translational inhibition. miRNA is known to play an important role in the root development and environmental responses in both the Arabidopsis and rice. However, little information is available to f...

journal_title：Plant biotechnology journal

authors： Kong X,Zhang M,Xu X,Li X,Li C,Ding Z

更新日期：2014-10-01 00:00:00

abstract：:Triterpenes are thirty-carbon compounds derived from the universal five-carbon prenyl precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Normally, triterpenes are synthesized via the mevalonate (MVA) pathway operating in the cytoplasm of eukaryotes where DMAPP is condensed with two IPPs to...

journal_title：Plant biotechnology journal

authors： Kempinski C,Jiang Z,Zinck G,Sato SJ,Ge Z,Clemente TE,Chappell J

更新日期：2019-02-01 00:00:00

abstract：:Plant chloroplasts are promising vehicles for recombinant protein production, but the process of protein folding in these organelles is not well understood in comparison with that in prokaryotic systems, such as Escherichia coli. This is particularly true for disulphide bond formation which is crucial for the biologic...

journal_title：Plant biotechnology journal

authors： Bally J,Paget E,Droux M,Job C,Job D,Dubald M

更新日期：2008-01-01 00:00:00

abstract：:Considered responsible for one million deaths in Ireland and widespread famine in the European continent during the 1840s, late blight, caused by Phytophthora infestans, remains the most devastating disease of potato (Solanum tuberosum L.) with about 15%-30% annual yield loss in sub-Saharan Africa, affecting mainly sm...

journal_title：Plant biotechnology journal

authors： Ghislain M,Byarugaba AA,Magembe E,Njoroge A,Rivera C,Román ML,Tovar JC,Gamboa S,Forbes GA,Kreuze JF,Barekye A,Kiggundu A

更新日期：2019-06-01 00:00:00

abstract：:Drought stress conditions decrease maize growth and yield, and aggravate preharvest aflatoxin contamination. While several studies have been performed on mature kernels responding to drought stress, the metabolic profiles of developing kernels are not as well characterized, particularly in germplasm with contrasting r...

journal_title：Plant biotechnology journal

authors： Yang L,Fountain JC,Ji P,Ni X,Chen S,Lee RD,Kemerait RC,Guo B

更新日期：2018-02-12 00:00:00

abstract：:Single-stranded oligonucleotides (ssODNs) can be used to direct the exchange of a single nucleotide or the repair of a single base within the coding region of a gene in a process that is known, generically, as gene editing. These molecules are composed of either all DNA residues or a mixture of RNA and DNA bases and u...

journal_title：Plant biotechnology journal

authors： Rivera-Torres N,Kmiec EB

更新日期：2016-02-01 00:00:00

abstract：:Transcription factors have been shown to control the activity of multiple stress response genes in a coordinated manner and therefore represent attractive targets for application in molecular plant breeding. We investigated the possibility of modulating the transcriptional regulation of drought and cold responses in t...

journal_title：Plant biotechnology journal

authors： Morran S,Eini O,Pyvovarenko T,Parent B,Singh R,Ismagul A,Eliby S,Shirley N,Langridge P,Lopato S

更新日期：2011-02-01 00:00:00

abstract：:Downy mildew (Plasmopara halstedii (Farl.) Berlese et de Toni) is a serious foliar pathogen of cultivated sunflower (Helianthus annuus L.). Genetic resistance is conditioned by several linked downy mildew resistance gene specificities in the HaRGC1 cluster of TIR-NBS-LRR resistance gene candidates (RGCs) on linkage gr...

journal_title：Plant biotechnology journal

authors： Slabaugh MB,Yu JK,Tang S,Heesacker A,Hu X,Lu G,Bidney D,Han F,Knapp SJ

更新日期：2003-05-01 00:00:00

abstract：:The plant immune system involves detection of pathogens via both cell-surface and intracellular receptors. Both receptor classes can induce transcriptional reprogramming that elevates disease resistance. To assess differential gene expression during plant immunity, we developed and deployed quantitative sequence captu...

journal_title：Plant biotechnology journal

authors： Ding P,Ngou BPM,Furzer OJ,Sakai T,Shrestha RK,MacLean D,Jones JDG

更新日期：2020-07-01 00:00:00

abstract：:Measurement of gene expression can provide important clues about gene function and molecular basis of developmental processes. Here, we have analysed the chickpea transcriptome in vegetative and flower tissues by exploiting the potential of high-throughput sequencing to measure gene expression. We mapped more than 295...

journal_title：Plant biotechnology journal

authors： Singh VK,Garg R,Jain M

更新日期：2013-08-01 00:00:00

abstract：:Despite the declarations and collective measures taken to eradicate hunger at World Food Summits, food security remains one of the biggest issues that we are faced with. The current scenario could worsen due to the alarming increase in world population, further compounded by adverse climatic conditions, such as increa...

journal_title：Plant biotechnology journal

authors： Singh J,Pandey P,James D,Chandrasekhar K,Achary VM,Kaul T,Tripathy BC,Reddy MK

更新日期：2014-12-01 00:00:00

abstract：:Which factors limit metabolite accumulation in plant cells? Are theories on flux control effective at explaining the results? Many biotechnologists cling to the idea that every pathway has a rate limiting enzyme and target such enzymes first in order to modulate fluxes. This often translates into large effects on meta...

journal_title：Plant biotechnology journal

authors： Morandini P

更新日期：2013-02-01 00:00:00

abstract：:Starch is an important renewable raw material with an increasing number of applications. Several attempts have been made to obtain plants that produce modified versions of starch or higher starch yield. Most of the approaches designed to increase the levels of starch have focused on the increment of the amount of ADP-...

journal_title：Plant biotechnology journal

authors： Gámez-Arjona FM,Li J,Raynaud S,Baroja-Fernández E,Muñoz FJ,Ovecka M,Ragel P,Bahaji A,Pozueta-Romero J,Mérida Á

更新日期：2011-12-01 00:00:00

abstract：:Eastern cottonwood (Populus deltoides Bartr. ex Marsh.) trees were engineered to express merA (mercuric ion reductase) and merB (organomercury lyase) transgenes in order to be used for the phytoremediation of mercury-contaminated soils. Earlier studies with Arabidopsis thaliana and Nicotiana tabacum showed that this g...

journal_title：Plant biotechnology journal

authors： Lyyra S,Meagher RB,Kim T,Heaton A,Montello P,Balish RS,Merkle SA

更新日期：2007-03-01 00:00:00

abstract：:Designing transformation experiments for either functional genomics or crop improvement requires knowledge of the transgene locus structure, number, transmission and expression resulting from a specific transformation method. We recently reported an improvement to the soybean [Glycine max (L.) Merrill] cotyledonary-no...

journal_title：Plant biotechnology journal

authors： Olhoft PM,Flagel LE,Somers DA

更新日期：2004-07-01 00:00:00

abstract：:Flowerless trait is highly desirable for poplar because it can prevent pollen- and seed-mediated transgene flow. We have isolated the second intron of PTAG2, an AGAMOUS (AG) orthologue from Populus trichocarpa. By fusing this intron sequence to a minimal 35S promoter sequence, we created two artificial promoters, fPTA...

journal_title：Plant biotechnology journal

authors： Li W,Hu W,Fang C,Chen L,Zhuang W,Katin-Grazzini L,McAvoy RJ,Guillard K,Li Y

更新日期：2016-12-01 00:00:00

abstract：:Lutein and zeaxanthin cannot be synthesized de novo in humans, and although lutein is abundant in fruit and vegetables, good dietary sources of zeaxanthin are scarce. Certain corn varieties provide adequate amounts because the ratio of endosperm β:ε lycopene cyclase activity favours the β-carotene/zeaxanthin branch of...

journal_title：Plant biotechnology journal

authors： Naqvi S,Zhu C,Farre G,Sandmann G,Capell T,Christou P

更新日期：2011-04-01 00:00:00

abstract：:Next-generation sequencing of complete genomes has given researchers unprecedented levels of information to study the multifaceted evolutionary changes that have shaped elite plant germplasm. In conjunction with population genetic analytical techniques and detailed online databases, we can more accurately capture the ...

journal_title：Plant biotechnology journal

authors： Campbell BC,Gilding EK,Mace ES,Tai S,Tao Y,Prentis PJ,Thomelin P,Jordan DR,Godwin ID

更新日期：2016-12-01 00:00:00