High-Spatiotemporal-Resolution Transcriptomes Provide Insights into Fruit Development and Ripening in Citrus sinensis.

Abstract:

:Citrus fruit has a unique structure with soft leathery peel and pulp containing vascular bundles and several segments with many juice sacs. The function and morphology of each fruit tissue are different. Therefore, analysis at the organ-wide or mixed-tissue level inevitably obscures many tissue-specific phenomena. High-throughput RNA sequencing was used to profile Citrus sinensis fruit development based on four fruit tissue types and six development stages from young fruits to ripe fruits. Using a coexpression network analysis, modules of coexpressed genes and hub genes of tissue-specific networks were identified. Of particular importance is the discovery of the regulatory network of phytohormones during citrus fruit development and ripening. A model was proposed to illustrate how ABF2 mediates the ABA signalling involved in sucrose transport, chlorophyll degradation, auxin homeostasis, carotenoid and ABA biosynthesis, and cell wall metabolism during citrus fruit development. Moreover, we depicted the detailed spatiotemporal expression patterns of the genes involved in sucrose and citric acid metabolism in citrus fruit and identified several key genes that may play crucial roles in sucrose and citric acid accumulation in the juice sac, such as SWEET15 and CsPH8. The high spatial and temporal resolution of our data provide important insights into the molecular networks underlying citrus fruit development and ripening.

journal_name

Plant Biotechnol J

authors

Feng G,Wu J,Xu Y,Lu L,Yi H

doi

10.1111/pbi.13549

subject

Has Abstract

pub_date

2021-01-20 00:00:00

eissn

1467-7644

issn

1467-7652

pub_type

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