NMR profiling of transgenic peas.

abstract：:Expression of the cecropin A gene in rice confers resistance to the rice blast fungus Magnaporthe oryzae. In this study, a polymerase chain reaction-based suppression subtractive hybridization approach was used to generate a cDNA macroarray from the elite japonica rice (Oryza sativa L.) cultivar 'Senia'. Gene expressi...

journal_title：Plant biotechnology journal

authors： Campo S,Manrique S,García-Martínez J,San Segundo B

更新日期：2008-08-01 00:00:00

abstract：:The production of biodegradable polymers that can be used to substitute petrochemical compounds in commercial products in transgenic plants is an important challenge for plant biotechnology. Nevertheless, it is often accompanied by reduced plant fitness. To decrease the phenotypic abnormalities of the sprout and to in...

journal_title：Plant biotechnology journal

authors： Hühns M,Neumann K,Hausmann T,Klemke F,Lockau W,Kahmann U,Kopertekh L,Staiger D,Pistorius EK,Reuther J,Waldvogel E,Wohlleben W,Effmert M,Junghans H,Neubauer K,Kragl U,Schmidt K,Schmidtke J,Broer I

更新日期：2009-12-01 00:00:00

abstract：:Next-generation sequencing of complete genomes has given researchers unprecedented levels of information to study the multifaceted evolutionary changes that have shaped elite plant germplasm. In conjunction with population genetic analytical techniques and detailed online databases, we can more accurately capture the ...

journal_title：Plant biotechnology journal

authors： Campbell BC,Gilding EK,Mace ES,Tai S,Tao Y,Prentis PJ,Thomelin P,Jordan DR,Godwin ID

更新日期：2016-12-01 00:00:00

abstract：:Spike lavender (Lavandula latifolia) essential oil is widely used in the perfume, cosmetic, flavouring and pharmaceutical industries. Thus, modifications of yield and composition of this essential oil by genetic engineering should have important scientific and commercial applications. We generated transgenic spike lav...

journal_title：Plant biotechnology journal

authors： Muñoz-Bertomeu J,Sales E,Ros R,Arrillaga I,Segura J

更新日期：2007-11-01 00:00:00

abstract：:Ascorbate is a powerful antioxidant in plants, and its levels are an important quality criteria in commercial species. Factors influencing these levels include environmental variations, particularly light, and the genetic control of its biosynthesis, recycling and degradation. One of the genes involved in the recyclin...

journal_title：Plant biotechnology journal

authors： Gest N,Garchery C,Gautier H,Jiménez A,Stevens R

更新日期：2013-04-01 00:00:00

abstract：:The development and adoption of hybrid seed technology have led to dramatic increases in agricultural productivity. However, it has been a challenge to develop a commercially viable platform for the production of hybrid wheat (Triticum aestivum) seed due to wheat's strong inbreeding habit. Recently, a novel platform f...

journal_title：Plant biotechnology journal

authors： Okada A,Arndell T,Borisjuk N,Sharma N,Watson-Haigh NS,Tucker EJ,Baumann U,Langridge P,Whitford R

更新日期：2019-10-01 00:00:00

abstract：:S-adenosyl-L-methionine (SAM) synthetase is the key enzyme involved in the biosynthesis of SAM, which serves as a common precursor for polyamines (PAs) and ethylene. A SAM synthetase cDNA (SlSAMS1) was introduced into the tomato genome using the Agrobacterium tumefaciens transformation method. Transgenic plants overex...

journal_title：Plant biotechnology journal

authors： Gong B,Li X,VandenLangenberg KM,Wen D,Sun S,Wei M,Li Y,Yang F,Shi Q,Wang X

更新日期：2014-08-01 00:00:00

abstract：:Expression of industrial enzymes in transgenic plants offers an alternative system to fungal fermentation for large-scale production. Very high levels of expression are required to make the enzymes cost-effective. We tested several parameters to determine the best method for achieving high levels of expression for a f...

journal_title：Plant biotechnology journal

authors： Hood EE,Bailey MR,Beifuss K,Magallanes-Lundback M,Horn ME,Callaway E,Drees C,Delaney DE,Clough R,Howard JA

更新日期：2003-03-01 00:00:00

abstract：:The rice α-amylase 3D promoter system, which is activated under sucrose-starved conditions, has emerged as a useful system for producing recombinant proteins. However, using rice as the production system for therapeutic proteins requires modifications of the N-glycosylation pattern because of the potential immunogenic...

journal_title：Plant biotechnology journal

authors： Shin YJ,Chong YJ,Yang MS,Kwon TH

更新日期：2011-12-01 00:00:00

abstract：:The activation of phospholipase Dα1 (PLDα1) produces lipid messenger phosphatidic acid and promotes stomatal closure in Arabidopsis. To explore the use of the PLDα1-mediated signalling towards decreasing water loss in crop plants, we introduced Arabidopsis PLDα1 under the control of a guard cell-specific promoter AtKa...

journal_title：Plant biotechnology journal

authors： Lu S,Bahn SC,Qu G,Qin H,Hong Y,Xu Q,Zhou Y,Hong Y,Wang X

更新日期：2013-04-01 00:00:00

abstract：:Designing transformation experiments for either functional genomics or crop improvement requires knowledge of the transgene locus structure, number, transmission and expression resulting from a specific transformation method. We recently reported an improvement to the soybean [Glycine max (L.) Merrill] cotyledonary-no...

journal_title：Plant biotechnology journal

authors： Olhoft PM,Flagel LE,Somers DA

更新日期：2004-07-01 00:00:00

abstract：:Co-expression of protease inhibitors like the tomato cystatin SlCYS8 is useful to increase recombinant protein production in plants, but key proteases involved in protein proteolysis are still unknown. Here, we performed activity-based protein profiling to identify proteases that are inhibited by SlCYS8 in agroinfiltr...

journal_title：Plant biotechnology journal

authors： Jutras PV,Grosse-Holz F,Kaschani F,Kaiser M,Michaud D,van der Hoorn RAL

更新日期：2019-08-01 00:00:00

abstract：:Genetic improvement of fibre quality is one of the main breeding goals for the upland cotton, Gossypium hirsutum, but there are difficulties with precise selection of traits. Therefore, it is important to improve the understanding of the genetic basis of phenotypic variation. In this study, we conducted phenotyping an...

journal_title：Plant biotechnology journal

authors： Sun Z,Wang X,Liu Z,Gu Q,Zhang Y,Li Z,Ke H,Yang J,Wu J,Wu L,Zhang G,Zhang C,Ma Z

更新日期：2017-08-01 00:00:00

abstract：:A dual-marker plasmid containing the selectable marker gene, manA, and the reporter gene, sgfp, was used to transform immature sorghum embryos by employing an Agrobacterium-mediated system. Both genes were under the control of the ubi1 promoter in a binary vector pPZP201. The Escherichia coli phosphomannose isomerase ...

journal_title：Plant biotechnology journal

authors： Gao Z,Xie X,Ling Y,Muthukrishnan S,Liang GH

更新日期：2005-11-01 00:00:00

abstract：:An important goal in biotechnological research is to improve the yield of crop plants. Here, we genetically modified simultaneously source and sink capacities in potato (Solanum tuberosum cv. Desirée) plants to improve starch yield. Source capacity was increased by mesophyll-specific overexpression of a pyrophosphatas...

journal_title：Plant biotechnology journal

authors： Jonik C,Sonnewald U,Hajirezaei MR,Flügge UI,Ludewig F

更新日期：2012-12-01 00:00:00

abstract：:Lignocellulosic feedstocks can be converted to biofuels, which can conceivably replace a large fraction of fossil fuels currently used for transformation. However, lignin, a prominent constituent of secondary cell walls, is an impediment to the conversion of cell walls to fuel: the recalcitrance problem. Biomass pretr...

journal_title：Plant biotechnology journal

authors： Poovaiah CR,Nageswara-Rao M,Soneji JR,Baxter HL,Stewart CN Jr

更新日期：2014-12-01 00:00:00

abstract：:Methods based on single nucleotide polymorphism (SNP), copy number variation (CNV) and presence/absence variation (PAV) discovery provide a valuable resource to study gene structure and evolution. However, as a result of these structural variations, a single reference genome is unable to cover the entire gene content ...

journal_title：Plant biotechnology journal

authors： Dolatabadian A,Bayer PE,Tirnaz S,Hurgobin B,Edwards D,Batley J

更新日期：2020-04-01 00:00:00

abstract：:Advances in the genetic transformation of cereals have improved the prospects of using biotechnology for plant improvement, and a toolbox of promoters with defined specificities would be a valuable resource in controlling the expression of transgenes in desired tissues for both plant improvement and molecular farming....

journal_title：Plant biotechnology journal

authors： Furtado A,Henry RJ,Pellegrineschi A

更新日期：2009-04-01 00:00:00

abstract：:The related A genome species of the Oryza genus are the effective gene pool for rice. Here, we report draft genomes for two Australian wild A genome taxa: O. rufipogon-like population, referred to as Taxon A, and O. meridionalis-like population, referred to as Taxon B. These two taxa were sequenced and assembled by in...

journal_title：Plant biotechnology journal

authors： Brozynska M,Copetti D,Furtado A,Wing RA,Crayn D,Fox G,Ishikawa R,Henry RJ

更新日期：2017-06-01 00:00:00

abstract：:Flowerless trait is highly desirable for poplar because it can prevent pollen- and seed-mediated transgene flow. We have isolated the second intron of PTAG2, an AGAMOUS (AG) orthologue from Populus trichocarpa. By fusing this intron sequence to a minimal 35S promoter sequence, we created two artificial promoters, fPTA...

journal_title：Plant biotechnology journal

authors： Li W,Hu W,Fang C,Chen L,Zhuang W,Katin-Grazzini L,McAvoy RJ,Guillard K,Li Y

更新日期：2016-12-01 00:00:00

abstract：:In the last 20 years, stem rust caused by the fungus Puccinia graminis f. sp. tritici (Pgt), has re-emerged as a major threat to wheat and barley production in Africa and Europe. In contrast to wheat with 60 designated stem rust (Sr) resistance genes, barley's genetic variation for stem rust resistance is very narrow ...

journal_title：Plant biotechnology journal

authors： Hatta MAM,Arora S,Ghosh S,Matny O,Smedley MA,Yu G,Chakraborty S,Bhatt D,Xia X,Steuernagel B,Richardson T,Mago R,Lagudah ES,Patron NJ,Ayliffe M,Rouse MN,Harwood WA,Periyannan S,Steffenson BJ,Wulff BBH

更新日期：2020-08-03 00:00:00

abstract：:Tomato is one of the most cultivated vegetables in the world and an important ingredient of the human diet. Tomato breeders and growers face a continuous challenge of combining high quantity (production volume) with high quality (appearance, taste and perception for the consumers, processing quality for the processing...

journal_title：Plant biotechnology journal

authors： Czerednik A,Busscher M,Angenent GC,de Maagd RA

更新日期：2015-02-01 00:00:00

abstract：:Nanobodies® (VHHs) provide powerful tools in therapeutic and biotechnological applications. Nevertheless, for some applications, bivalent antibodies perform much better, and for this, an Fc chain can be fused to the VHH domain, resulting in a bivalent homodimeric VHH-Fc complex. However, the production of bivalent ant...

journal_title：Plant biotechnology journal

authors： De Buck S,Nolf J,De Meyer T,Virdi V,De Wilde K,Van Lerberge E,Van Droogenbroeck B,Depicker A

更新日期：2013-10-01 00:00:00

abstract：:The rice root system is important for growth. The crosstalk between auxin and cytokinin mediates root initiation and elongation. However, it remains unclear how the transcriptional network upstream of the auxin and cytokinin signalling pathways determines root development. Here, we observed that the knockdown of OsNAC...

journal_title：Plant biotechnology journal

authors： Mao C,He J,Liu L,Deng Q,Yao X,Liu C,Qiao Y,Li P,Ming F

更新日期：2020-02-01 00:00:00

abstract：:The plant pathogen, Pseudomonas syringae (Ps), together with related Ps species, infects and attacks a wide range of agronomically important crops, including tomato, kiwifruit, pepper, olive and soybean, causing economic losses. Currently, chemicals and introduced resistance genes are used to protect plants against th...

journal_title：Plant biotechnology journal

authors： Rooney WM,Grinter RW,Correia A,Parkhill J,Walker DC,Milner JJ

更新日期：2020-05-01 00:00:00

abstract：:Suppression of the microsomal ω6 oleate desaturase during the seed development of soybean (Glycine max) with the 420-bp soybean FAD2-1A intron as RNAi trigger shifts the conventional fatty acid composition of soybean oil from 20% oleic and 60% polyunsaturates to one containing greater than 80% oleic acid and less than...

journal_title：Plant biotechnology journal

authors： Wagner N,Mroczka A,Roberts PD,Schreckengost W,Voelker T

更新日期：2011-09-01 00:00:00

abstract：:Grain size and plant architecture are critical factors determining crop productivity. Here, we performed gene editing of the MIR396 gene family in rice and found that MIR396e and MIR396f are two important regulators of grain size and plant architecture. mir396ef mutations can increase grain yield by increasing grain s...

journal_title：Plant biotechnology journal

authors： Miao C,Wang D,He R,Liu S,Zhu JK

更新日期：2020-02-01 00:00:00

abstract：:Orobanche spp. (broomrape) are parasitic plants which subsist on the roots of a wide range of hosts, including tomato, causing severe losses in yield quality and quantity. Large amounts of mannitol accumulate in this parasitic weed during development. Mannose 6-phosphate reductase (M6PR) is a key enzyme in mannitol bi...

journal_title：Plant biotechnology journal

authors： Aly R,Cholakh H,Joel DM,Leibman D,Steinitz B,Zelcer A,Naglis A,Yarden O,Gal-On A

更新日期：2009-08-01 00:00:00

abstract：:The assembly of a reference genome sequence of bread wheat is challenging due to its specific features such as the genome size of 17 Gbp, polyploid nature and prevalence of repetitive sequences. BAC-by-BAC sequencing based on chromosomal physical maps, adopted by the International Wheat Genome Sequencing Consortium as...

journal_title：Plant biotechnology journal

authors： Staňková H,Hastie AR,Chan S,Vrána J,Tulpová Z,Kubaláková M,Visendi P,Hayashi S,Luo M,Batley J,Edwards D,Doležel J,Šimková H

更新日期：2016-07-01 00:00:00

abstract：:Genetic engineering with just a few genes has changed agriculture in the last 20 years. The most frequently used transgenes are the herbicide resistance genes for efficient weed control and the Bt toxin genes for insect resistance. The adoption of the first-generation genetically engineered crops has been very success...

journal_title：Plant biotechnology journal

authors： Yu W,Yau YY,Birchler JA

更新日期：2016-05-01 00:00:00