NMR profiling of transgenic peas.

Abstract:

:A high throughput proton nuclear magnetic resonance spectroscopy method for the metabolite fingerprinting of plants was applied to genetically modified peas (Pisum sativum) to determine whether biochemical changes, so called 'unintended effects', beyond those intended by incorporation of a transgene, were detectable. Multivariate analysis of 1H NMR (nuclear magnetic resonance) spectra obtained from uniformly grown glasshouse plants revealed differences between the transgenic and control group that exceeded the natural variation of the plants. When a larger data set of six related transgenic lines was analysed, including a null segregant in addition to the wild-type control, multivariate analysis showed that the distribution of metabolites in the transgenics was different from that of the null segregant. However, the profile obtained from the wild-type material was diverse in comparison with both the transgenics and the null segregant, suggesting that the primary cause of the observed differences was that the transformation process selects for a subset of individuals able to undergo the transformation and selection procedures, and that their descendants have a restricted variation in metabolite profile, rather than that the presence of the transgene itself generates these differences.

journal_name

Plant Biotechnol J

authors

Charlton A,Allnutt T,Holmes S,Chisholm J,Bean S,Ellis N,Mullineaux P,Oehlschlager S

doi

10.1046/j.1467-7652.2003.00045.x

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

27-35

issue

1

eissn

1467-7644

issn

1467-7652

pii

PBI045

journal_volume

2

pub_type

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