Generation of marker-free transgenic hexaploid wheat via an Agrobacterium-mediated co-transformation strategy in commercial Chinese wheat varieties.

abstract：:Drought conditions limit agricultural production by preventing crops from reaching their genetically predetermined maximum yields. Here, we present the results of field evaluations of rice overexpressing OsNAC9, a member of the rice NAC domain family. Root-specific (RCc3) and constitutive (GOS2) promoters were used to...

journal_title：Plant biotechnology journal

authors： Redillas MC,Jeong JS,Kim YS,Jung H,Bang SW,Choi YD,Ha SH,Reuzeau C,Kim JK

更新日期：2012-09-01 00:00:00

abstract：:China is the origin and evolutionary centre of Oriental pears. Pyrus betuleafolia is a wild species native to China and distributed in the northern region, and it is widely used as rootstock. Here, we report the de novo assembly of the genome of P. betuleafolia-Shanxi Duli using an integrated strategy that combines Pa...

journal_title：Plant biotechnology journal

authors： Dong X,Wang Z,Tian L,Zhang Y,Qi D,Huo H,Xu J,Li Z,Liao R,Shi M,Wahocho SA,Liu C,Zhang S,Tian Z,Cao Y

更新日期：2020-02-01 00:00:00

abstract：:N-glycosylation is critical for recombinant glycoprotein production as it influences the heterogeneity of products and affects their biological function. In most eukaryotes, the oligosaccharyltransferase is the central-protein complex facilitating the N-glycosylation of proteins in the lumen of the endoplasmic reticul...

journal_title：Plant biotechnology journal

authors： Castilho A,Beihammer G,Pfeiffer C,Göritzer K,Montero-Morales L,Vavra U,Maresch D,Grünwald-Gruber C,Altmann F,Steinkellner H,Strasser R

更新日期：2018-10-01 00:00:00

abstract：:In a de novo genotyping-by-sequencing (GBS) analysis of short, 64-base tag-level haplotypes in 4657 accessions of cultivated oat, we discovered 164741 tag-level (TL) genetic variants containing 241224 SNPs. From this, the marker density of an oat consensus map was increased by the addition of more than 70000 loci. The...

journal_title：Plant biotechnology journal

authors： Bekele WA,Wight CP,Chao S,Howarth CJ,Tinker NA

更新日期：2018-08-01 00:00:00

abstract：:GM crops are the most studied crops in history. Approximately 5% of the safety studies on them show adverse effects that are a cause for concern and tend to be featured in media reports. Although these reports are based on just a handful of GM events, they are used to cast doubt on all GM crops. Furthermore, they tend...

journal_title：Plant biotechnology journal

authors： Sánchez MA,Parrott WA

更新日期：2017-10-01 00:00:00

abstract：:Pollen germination and pollen tube growth are important physiological processes of sexual reproduction of plants and also are involved in signal transduction. Our previous study reveals that ZmSTK1 and ZmSTK2 are two receptor-like cytoplasmic kinases (RLCK) homologs in Zea mays as members of receptor-like protein kina...

journal_title：Plant biotechnology journal

authors： Fan M,Zhang C,Shi L,Liu C,Ma W,Chen M,Liu K,Cai F,Wang G,Wei Z,Jiang M,Liu Z,Javeed A,Lin F

更新日期：2018-08-01 00:00:00

abstract：:Steviol glycosides (SGs) are extracted from Stevia leaves for use as a natural sweetener. Among SGs, stevioside is most abundant in leaf extracts followed by rebaudioside A (Reb A). However, Reb A is of particular interest because of its sweeter and more pleasant taste compared to stevioside. Therefore, the developmen...

journal_title：Plant biotechnology journal

authors： Kim MJ,Zheng J,Liao MH,Jang IC

更新日期：2019-06-01 00:00:00

abstract：:Drought stress conditions decrease maize growth and yield, and aggravate preharvest aflatoxin contamination. While several studies have been performed on mature kernels responding to drought stress, the metabolic profiles of developing kernels are not as well characterized, particularly in germplasm with contrasting r...

journal_title：Plant biotechnology journal

authors： Yang L,Fountain JC,Ji P,Ni X,Chen S,Lee RD,Kemerait RC,Guo B

更新日期：2018-02-12 00:00:00

abstract：:Single-chain variable fragments (scFvs), as well as other recombinant antibodies, have become powerful tools for therapy and analysis in both human and veterinary medicine. Transgenic plants have been developed as an efficient production system for these recombinant proteins, achieving high yields at moderate cost. Se...

journal_title：Plant biotechnology journal

authors： Scheller J,Leps M,Conrad U

更新日期：2006-03-01 00:00:00

abstract：:Phytochrome-interacting factors (PIFs) play major roles in regulating plant growth and development, but their roles in drought stress remain elusive. Here, we cloned and characterized a maize (Zea mays) PIF transcription factor, ZmPIF1. The expression level of ZmPIF1 was significantly induced by independent drought an...

journal_title：Plant biotechnology journal

authors： Gao Y,Wu M,Zhang M,Jiang W,Ren X,Liang E,Zhang D,Zhang C,Xiao N,Li Y,Dai Y,Chen J

更新日期：2018-07-01 00:00:00

abstract：:Menadione sodium bisulphite (MSB) is a water-soluble derivative of vitamin K3, or menadione, and has been previously demonstrated to function as a plant defence activator against several pathogens in several plant species. However, there are no reports of the role of this vitamin in the induction of resistance in the ...

journal_title：Plant biotechnology journal

authors： Borges AA,Dobon A,Expósito-Rodríguez M,Jiménez-Arias D,Borges-Pérez A,Casañas-Sánchez V,Pérez JA,Luis JC,Tornero P

更新日期：2009-10-01 00:00:00

abstract：:The plant immune system involves detection of pathogens via both cell-surface and intracellular receptors. Both receptor classes can induce transcriptional reprogramming that elevates disease resistance. To assess differential gene expression during plant immunity, we developed and deployed quantitative sequence captu...

journal_title：Plant biotechnology journal

authors： Ding P,Ngou BPM,Furzer OJ,Sakai T,Shrestha RK,MacLean D,Jones JDG

更新日期：2020-07-01 00:00:00

abstract：:Globally, wheat is the most widely grown crop and one of the three most important crops for human and livestock feed. However, the complex nature of the wheat genome has, until recently, resulted in a lack of single nucleotide polymorphism (SNP)-based molecular markers of practical use to wheat breeders. Recently, lar...

journal_title：Plant biotechnology journal

authors： Allen AM,Barker GL,Wilkinson P,Burridge A,Winfield M,Coghill J,Uauy C,Griffiths S,Jack P,Berry S,Werner P,Melichar JP,McDougall J,Gwilliam R,Robinson P,Edwards KJ

更新日期：2013-04-01 00:00:00

abstract：:Multivitamin corn is a novel genetically engineered variety that simultaneously produces high levels of β-carotene, ascorbate and folate, and therefore has the potential to address simultaneously multiple micronutrient deficiencies caused by the lack of vitamins A, B9 and C in developing country populations. As part o...

journal_title：Plant biotechnology journal

authors： Arjó G,Capell T,Matias-Guiu X,Zhu C,Christou P,Piñol C

更新日期：2012-12-01 00:00:00

abstract：:The early methionine (Em) proteins are members of the late embryogenesis abundant (LEA) group of proteins that have been considered to be embryo specific. The ability of a 646-bp wheat Em promoter to control green fluorescent protein (gfp) expression was investigated in transgenic barley and rice. Seeds of transgenic ...

journal_title：Plant biotechnology journal

authors： Furtado A,Henry RJ

更新日期：2005-07-01 00:00:00

abstract：:Modified fatty acids (mFA) have diverse uses; for example, cyclopropane fatty acids (CPA) are feedstocks for producing coatings, lubricants, plastics and cosmetics. The expression of mFA-producing enzymes in crop and model plants generally results in lower levels of mFA accumulation than in their natural-occurring sou...

journal_title：Plant biotechnology journal

authors： Yu XH,Cahoon RE,Horn PJ,Shi H,Prakash RR,Cai Y,Hearney M,Chapman KD,Cahoon EB,Schwender J,Shanklin J

更新日期：2018-04-01 00:00:00

abstract：:The rapid accumulation of defensive transgene products in plants only on pathogen invasion has clear advantages over their constitutive synthesis. In this study, two antimicrobial peptides from the skin secretions of frogs, MsrA2 (N-methionine-dermaseptin B1) and temporin A, were evaluated for engineering pathogen-ind...

journal_title：Plant biotechnology journal

authors： Yevtushenko DP,Misra S

更新日期：2007-11-01 00:00:00

abstract：:Anthocyanins have crucial biological functions and affect quality of horticultural produce. Anthocyanins accumulate in ripe peach fruit; differential accumulation is observed in deep coloured cultivar 'Hujingmilu' and lightly pigmented cultivar 'Yulu'. The difference was not fully explained by accumulation of total fl...

journal_title：Plant biotechnology journal

authors： Zhao Y,Dong W,Zhu Y,Allan AC,Lin-Wang K,Xu C

更新日期：2020-05-01 00:00:00

abstract：:We constructed a novel autonomously replicating gene expression shuttle vector, with the aim of developing a system for transiently expressing proteins at levels useful for commercial production of vaccines and other proteins in plants. The vector, pRIC, is based on the mild strain of the geminivirus Bean yellow dwarf...

journal_title：Plant biotechnology journal

authors： Regnard GL,Halley-Stott RP,Tanzer FL,Hitzeroth II,Rybicki EP

更新日期：2010-01-01 00:00:00

abstract：:Developing new strategies for crop plants to respond to drought is crucial for their innovative breeding. The down-regulation of nuclear cap-binding proteins in Arabidopsis renders plants drought tolerant. The CBP80 gene in the potato cultivar Desiree was silenced using artificial microRNAs. Transgenic plants displaye...

journal_title：Plant biotechnology journal

authors： Pieczynski M,Marczewski W,Hennig J,Dolata J,Bielewicz D,Piontek P,Wyrzykowska A,Krusiewicz D,Strzelczyk-Zyta D,Konopka-Postupolska D,Krzeslowska M,Jarmolowski A,Szweykowska-Kulinska Z

更新日期：2013-05-01 00:00:00

abstract：:Genetic improvement of fibre quality is one of the main breeding goals for the upland cotton, Gossypium hirsutum, but there are difficulties with precise selection of traits. Therefore, it is important to improve the understanding of the genetic basis of phenotypic variation. In this study, we conducted phenotyping an...

journal_title：Plant biotechnology journal

authors： Sun Z,Wang X,Liu Z,Gu Q,Zhang Y,Li Z,Ke H,Yang J,Wu J,Wu L,Zhang G,Zhang C,Ma Z

更新日期：2017-08-01 00:00:00

abstract：:Soya bean (Glycine max) and grass pea (Lathyrus sativus) seeds are important sources of dietary proteins; however, they also contain antinutritional metabolite oxalic acid (OA). Excess dietary intake of OA leads to nephrolithiasis due to the formation of calcium oxalate crystals in kidneys. Besides, OA is also a known...

journal_title：Plant biotechnology journal

authors： Kumar V,Chattopadhyay A,Ghosh S,Irfan M,Chakraborty N,Chakraborty S,Datta A

更新日期：2016-06-01 00:00:00

abstract：:Although a physiological role of heat-shock proteins (HSP) in antigen presentation and immune response activation has not been directly demonstrated, their use as vaccine components is under clinical trial. We have previously demonstrated that the structure of plant-derived HSP70 (pHSP70) can be superimposed to the ma...

journal_title：Plant biotechnology journal

authors： Buriani G,Mancini C,Benvenuto E,Baschieri S

更新日期：2012-04-01 00:00:00

abstract：:Designing transformation experiments for either functional genomics or crop improvement requires knowledge of the transgene locus structure, number, transmission and expression resulting from a specific transformation method. We recently reported an improvement to the soybean [Glycine max (L.) Merrill] cotyledonary-no...

journal_title：Plant biotechnology journal

authors： Olhoft PM,Flagel LE,Somers DA

更新日期：2004-07-01 00:00:00

abstract：:Receptor-like kinases (RLKs) are important components of plant innate immunity. Although recent studies have revealed that the RLK suppressor of BIR1-1 (SOBIR1) can interact with multiple receptor-like proteins and is required for resistance against fungal pathogens, how the signal is transduced and triggers immune re...

journal_title：Plant biotechnology journal

authors： Zhou Y,Sun L,Wassan GM,He X,Shaban M,Zhang L,Zhu L,Zhang X

更新日期：2019-01-01 00:00:00

abstract：:Measurement of gene expression can provide important clues about gene function and molecular basis of developmental processes. Here, we have analysed the chickpea transcriptome in vegetative and flower tissues by exploiting the potential of high-throughput sequencing to measure gene expression. We mapped more than 295...

journal_title：Plant biotechnology journal

authors： Singh VK,Garg R,Jain M

更新日期：2013-08-01 00:00:00

abstract：:Co-expression of protease inhibitors like the tomato cystatin SlCYS8 is useful to increase recombinant protein production in plants, but key proteases involved in protein proteolysis are still unknown. Here, we performed activity-based protein profiling to identify proteases that are inhibited by SlCYS8 in agroinfiltr...

journal_title：Plant biotechnology journal

authors： Jutras PV,Grosse-Holz F,Kaschani F,Kaiser M,Michaud D,van der Hoorn RAL

更新日期：2019-08-01 00:00:00

abstract：:Single-stranded oligonucleotides (ssODNs) can be used to direct the exchange of a single nucleotide or the repair of a single base within the coding region of a gene in a process that is known, generically, as gene editing. These molecules are composed of either all DNA residues or a mixture of RNA and DNA bases and u...

journal_title：Plant biotechnology journal

authors： Rivera-Torres N,Kmiec EB

更新日期：2016-02-01 00:00:00

abstract：:Traditional breeding methods are hindered in bananas due to the fact that major cultivars are sterile, parthenocarpic, triploid and thus clonally propagated. This has resulted in a narrow genetic base and limited resilience to biotic and abiotic stresses. Mutagenesis of in vitro propagated bananas is one method to int...

journal_title：Plant biotechnology journal

authors： Datta S,Jankowicz-Cieslak J,Nielen S,Ingelbrecht I,Till BJ

更新日期：2018-02-24 00:00:00

abstract：:S-adenosyl-L-methionine (SAM) synthetase is the key enzyme involved in the biosynthesis of SAM, which serves as a common precursor for polyamines (PAs) and ethylene. A SAM synthetase cDNA (SlSAMS1) was introduced into the tomato genome using the Agrobacterium tumefaciens transformation method. Transgenic plants overex...

journal_title：Plant biotechnology journal

authors： Gong B,Li X,VandenLangenberg KM,Wen D,Sun S,Wei M,Li Y,Yang F,Shi Q,Wang X

更新日期：2014-08-01 00:00:00