Simultaneous boosting of source and sink capacities doubles tuber starch yield of potato plants.

Abstract:

:An important goal in biotechnological research is to improve the yield of crop plants. Here, we genetically modified simultaneously source and sink capacities in potato (Solanum tuberosum cv. Desirée) plants to improve starch yield. Source capacity was increased by mesophyll-specific overexpression of a pyrophosphatase or, alternatively, by antisense expression of the ADP-glucose pyrophosphorylase in leaves. Both approaches make use of re-routing photoassimilates to sink organs at the expense of leaf starch accumulation. Simultaneous increase in sink capacity was accomplished by overexpression of two plastidic metabolite translocators, that is, a glucose 6-phosphate/phosphate translocator and an adenylate translocator in tubers. Employing such a 'pull' approach, we have previously shown that potato starch content and yield can be increased when sink strength is elevated. In the current biotechnological approach, we successfully enhanced source and sink capacities by a combination of 'pull' and 'push' approaches using two different attempts. A doubling in tuber starch yield was achieved. This successful approach might be transferable to other crop plants in the future.

journal_name

Plant Biotechnol J

authors

Jonik C,Sonnewald U,Hajirezaei MR,Flügge UI,Ludewig F

doi

10.1111/j.1467-7652.2012.00736.x

subject

Has Abstract

pub_date

2012-12-01 00:00:00

pages

1088-98

issue

9

eissn

1467-7644

issn

1467-7652

journal_volume

10

pub_type

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