Abstract:
:An important goal in biotechnological research is to improve the yield of crop plants. Here, we genetically modified simultaneously source and sink capacities in potato (Solanum tuberosum cv. Desirée) plants to improve starch yield. Source capacity was increased by mesophyll-specific overexpression of a pyrophosphatase or, alternatively, by antisense expression of the ADP-glucose pyrophosphorylase in leaves. Both approaches make use of re-routing photoassimilates to sink organs at the expense of leaf starch accumulation. Simultaneous increase in sink capacity was accomplished by overexpression of two plastidic metabolite translocators, that is, a glucose 6-phosphate/phosphate translocator and an adenylate translocator in tubers. Employing such a 'pull' approach, we have previously shown that potato starch content and yield can be increased when sink strength is elevated. In the current biotechnological approach, we successfully enhanced source and sink capacities by a combination of 'pull' and 'push' approaches using two different attempts. A doubling in tuber starch yield was achieved. This successful approach might be transferable to other crop plants in the future.
journal_name
Plant Biotechnol Jjournal_title
Plant biotechnology journalauthors
Jonik C,Sonnewald U,Hajirezaei MR,Flügge UI,Ludewig Fdoi
10.1111/j.1467-7652.2012.00736.xsubject
Has Abstractpub_date
2012-12-01 00:00:00pages
1088-98issue
9eissn
1467-7644issn
1467-7652journal_volume
10pub_type
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