Foreign gene products can be enhanced by introduction into low storage protein mutants.

abstract：:Approximately 20,000 of the rice-FOX Arabidopsis transgenic lines, which overexpress 13,000 rice full-length cDNAs at random in Arabidopsis, were screened for bacterial disease resistance by dip inoculation with Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). The identities of the overexpressed genes were determi...

journal_title：Plant biotechnology journal

authors： Dubouzet JG,Maeda S,Sugano S,Ohtake M,Hayashi N,Ichikawa T,Kondou Y,Kuroda H,Horii Y,Matsui M,Oda K,Hirochika H,Takatsuji H,Mori M

更新日期：2011-05-01 00:00:00

abstract：:The plant immune system involves detection of pathogens via both cell-surface and intracellular receptors. Both receptor classes can induce transcriptional reprogramming that elevates disease resistance. To assess differential gene expression during plant immunity, we developed and deployed quantitative sequence captu...

journal_title：Plant biotechnology journal

authors： Ding P,Ngou BPM,Furzer OJ,Sakai T,Shrestha RK,MacLean D,Jones JDG

更新日期：2020-07-01 00:00:00

abstract：:The hexaploid wheat (Triticum aestivum) adult plant resistance gene, Lr34/Yr18/Sr57/Pm38/Ltn1, provides broad-spectrum resistance to wheat leaf rust (Lr34), stripe rust (Yr18), stem rust (Sr57) and powdery mildew (Pm38) pathogens, and has remained effective in wheat crops for many decades. The partial resistance provi...

journal_title：Plant biotechnology journal

authors： Rinaldo A,Gilbert B,Boni R,Krattinger SG,Singh D,Park RF,Lagudah E,Ayliffe M

更新日期：2017-07-01 00:00:00

abstract：:Transgenic rice accumulating the modified major Japanese cedar pollen allergens, Cryptomeria japonica 1 (Cry j 1) and Cryptomeria japonica 2 (Cry j 2), which were deconstructed by fragmentation and shuffling, respectively, in the edible part of the seed was generated by transformation of a good-tasting rice variety, '...

journal_title：Plant biotechnology journal

authors： Wakasa Y,Takagi H,Hirose S,Yang L,Saeki M,Nishimura T,Kaminuma O,Hiroi T,Takaiwa F

更新日期：2013-01-01 00:00:00

abstract：:Salinity and drought are major environmental factors limiting the growth and productivity of alfalfa worldwide as this economically important legume forage is sensitive to these kinds of abiotic stress. In this study, transgenic alfalfa lines expressing both tonoplast NXH and H(+)-PPase genes, ZxNHX and ZxVP1-1 from t...

journal_title：Plant biotechnology journal

authors： Bao AK,Du BQ,Touil L,Kang P,Wang QL,Wang SM

更新日期：2016-03-01 00:00:00

abstract：:Heavy metal accumulation in the environment poses great risks to flora and fauna. However, monitoring sites prone to accumulation poses scale and economic challenges. In this study, we present and test a method for monitoring these sites using fluorescent resonance energy transfer (FRET) change in response to zinc (Zn...

journal_title：Plant biotechnology journal

authors： Adams JP,Adeli A,Hsu CY,Harkess RL,Page GP,Depamphilis CW,Schultz EB,Yuceer C

更新日期：2012-02-01 00:00:00

abstract：:Considered responsible for one million deaths in Ireland and widespread famine in the European continent during the 1840s, late blight, caused by Phytophthora infestans, remains the most devastating disease of potato (Solanum tuberosum L.) with about 15%-30% annual yield loss in sub-Saharan Africa, affecting mainly sm...

journal_title：Plant biotechnology journal

authors： Ghislain M,Byarugaba AA,Magembe E,Njoroge A,Rivera C,Román ML,Tovar JC,Gamboa S,Forbes GA,Kreuze JF,Barekye A,Kiggundu A

更新日期：2019-06-01 00:00:00

abstract：:A dual-marker plasmid containing the selectable marker gene, manA, and the reporter gene, sgfp, was used to transform immature sorghum embryos by employing an Agrobacterium-mediated system. Both genes were under the control of the ubi1 promoter in a binary vector pPZP201. The Escherichia coli phosphomannose isomerase ...

journal_title：Plant biotechnology journal

authors： Gao Z,Xie X,Ling Y,Muthukrishnan S,Liang GH

更新日期：2005-11-01 00:00:00

abstract：:The rapid accumulation of defensive transgene products in plants only on pathogen invasion has clear advantages over their constitutive synthesis. In this study, two antimicrobial peptides from the skin secretions of frogs, MsrA2 (N-methionine-dermaseptin B1) and temporin A, were evaluated for engineering pathogen-ind...

journal_title：Plant biotechnology journal

authors： Yevtushenko DP,Misra S

更新日期：2007-11-01 00:00:00

abstract：:Citrus fruit has a unique structure with soft leathery peel and pulp containing vascular bundles and several segments with many juice sacs. The function and morphology of each fruit tissue are different. Therefore, analysis at the organ-wide or mixed-tissue level inevitably obscures many tissue-specific phenomena. Hig...

journal_title：Plant biotechnology journal

authors： Feng G,Wu J,Xu Y,Lu L,Yi H

更新日期：2021-01-20 00:00:00

abstract：:The plant pathogen, Pseudomonas syringae (Ps), together with related Ps species, infects and attacks a wide range of agronomically important crops, including tomato, kiwifruit, pepper, olive and soybean, causing economic losses. Currently, chemicals and introduced resistance genes are used to protect plants against th...

journal_title：Plant biotechnology journal

authors： Rooney WM,Grinter RW,Correia A,Parkhill J,Walker DC,Milner JJ

更新日期：2020-05-01 00:00:00

abstract：:Plant chloroplasts are promising vehicles for recombinant protein production, but the process of protein folding in these organelles is not well understood in comparison with that in prokaryotic systems, such as Escherichia coli. This is particularly true for disulphide bond formation which is crucial for the biologic...

journal_title：Plant biotechnology journal

authors： Bally J,Paget E,Droux M,Job C,Job D,Dubald M

更新日期：2008-01-01 00:00:00

abstract：:Receptor-like kinases (RLKs) are important components of plant innate immunity. Although recent studies have revealed that the RLK suppressor of BIR1-1 (SOBIR1) can interact with multiple receptor-like proteins and is required for resistance against fungal pathogens, how the signal is transduced and triggers immune re...

journal_title：Plant biotechnology journal

authors： Zhou Y,Sun L,Wassan GM,He X,Shaban M,Zhang L,Zhu L,Zhang X

更新日期：2019-01-01 00:00:00

abstract：:Clustered regularly interspaced short palindromic repeat (CRISPR) and Cas9-associated protein systems provide a powerful genetic manipulation tool that can drive plant research forward. Nuclease-dead Cas9 (dCas9) is an enzymatically inactive mutant of Cas9 in which its endonuclease activity is non-functional. The appl...

journal_title：Plant biotechnology journal

authors： Moradpour M,Abdulah SNA

更新日期：2020-01-01 00:00:00

abstract：:Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is severely damaging to the global citrus industry. Targeted editing of host disease-susceptibility genes represents an interesting and potentially durable alternative in plant breeding for resistance. Here, we report improvement of citrus canker resistanc...

journal_title：Plant biotechnology journal

authors： Peng A,Chen S,Lei T,Xu L,He Y,Wu L,Yao L,Zou X

更新日期：2017-12-01 00:00:00

abstract：:Genotype specificity is a big problem lagging the development of efficient hexaploid wheat transformation system. Increasingly, the biosecurity of genetically modified organisms is garnering public attention, so the generation of marker-free transgenic plants is very important to the eventual potential commercial rele...

journal_title：Plant biotechnology journal

authors： Wang K,Liu H,Du L,Ye X

更新日期：2017-05-01 00:00:00

abstract：:Low grain moisture at harvest is crucial for safe production, transport, and storage, but the genetic architecture of this trait in maize (Zea mays) remains elusive. Here, we measured the dynamic changes in grain moisture content in an association mapping panel of 513 diverse maize inbred lines at five successive stag...

journal_title：Plant biotechnology journal

authors： Li W,Yu Y,Wang L,Luo Y,Peng Y,Xu Y,Liu X,Wu S,Jian L,Xu J,Xiao Y,Yan J

更新日期：2021-01-01 00:00:00

abstract：:Although it is one of the major crops in the world, corn has poor nutritional quality for human and animal consumption due to its low lysine content. Here, we report a method of simultaneous expression of a deregulated lysine biosynthetic enzyme, CordapA, and reduction of a bifunctional lysine degradation enzyme, lysi...

journal_title：Plant biotechnology journal

authors： Frizzi A,Huang S,Gilbertson LA,Armstrong TA,Luethy MH,Malvar TM

更新日期：2008-01-01 00:00:00

abstract：:Plant genetic engineering can create transgenic crops with improved characteristics by introducing trait genes through transformation. Appropriate regulatory elements such as promoters and terminators have to be present in certain configurations for the transgenes to be properly expressed. Five terminators native to s...

journal_title：Plant biotechnology journal

authors： Xing A,Moon BP,Mills KM,Falco SC,Li Z

更新日期：2010-09-01 00:00:00

abstract：:Although much explored, oral tolerance for treatment of autoimmune diseases still awaits the establishment of novel and effective vectors. We investigated whether the tolerogenic CTA1(R7K)-COL-DD fusion protein can be expressed in edible plants, to induce oral tolerance and protect against arthritis. The fusion protei...

journal_title：Plant biotechnology journal

authors： Hansson C,Schön K,Kalbina I,Strid Å,Andersson S,Bokarewa MI,Lycke NY

更新日期：2016-04-01 00:00:00

abstract：:The identification of genetic markers linked to genes of agronomic importance is a major aim of crop research and breeding programmes. Here, we identify markers for Yr15, a major disease resistance gene for wheat yellow rust, using a segregating F2 population. After phenotyping, we implemented RNA sequencing (RNA-Seq)...

journal_title：Plant biotechnology journal

authors： Ramirez-Gonzalez RH,Segovia V,Bird N,Fenwick P,Holdgate S,Berry S,Jack P,Caccamo M,Uauy C

更新日期：2015-06-01 00:00:00

abstract：:Genetically engineered (GE) rice lines expressing Lepidoptera-active insecticidal cry genes from the bacterium Bacillus thuringiensis (Bt) have been developed in China. Field surveys indicated that Bt rice harbours fewer rice planthoppers than non-Bt rice although planthoppers are not sensitive to the produced Bt Cry ...

journal_title：Plant biotechnology journal

authors： Wang X,Liu Q,Meissle M,Peng Y,Wu K,Romeis J,Li Y

更新日期：2018-10-01 00:00:00

abstract：:The production of biodegradable polymers that can be used to substitute petrochemical compounds in commercial products in transgenic plants is an important challenge for plant biotechnology. Nevertheless, it is often accompanied by reduced plant fitness. To decrease the phenotypic abnormalities of the sprout and to in...

journal_title：Plant biotechnology journal

authors： Hühns M,Neumann K,Hausmann T,Klemke F,Lockau W,Kahmann U,Kopertekh L,Staiger D,Pistorius EK,Reuther J,Waldvogel E,Wohlleben W,Effmert M,Junghans H,Neubauer K,Kragl U,Schmidt K,Schmidtke J,Broer I

更新日期：2009-12-01 00:00:00

abstract：:Glyphosate is a popular, systemic, broad-spectrum herbicide used in modern agriculture. Being a structural analog of phosphoenolpyruvate (PEP), it inhibits 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) which is responsible for the biosynthesis of aromatic amino acids and various aromatic secondary metabolites. T...

journal_title：Plant biotechnology journal

authors： Achary VMM,Sheri V,Manna M,Panditi V,Borphukan B,Ram B,Agarwal A,Fartyal D,Teotia D,Masakapalli SK,Agrawal PK,Reddy MK

更新日期：2020-12-01 00:00:00

abstract：:Measurement of gene expression can provide important clues about gene function and molecular basis of developmental processes. Here, we have analysed the chickpea transcriptome in vegetative and flower tissues by exploiting the potential of high-throughput sequencing to measure gene expression. We mapped more than 295...

journal_title：Plant biotechnology journal

authors： Singh VK,Garg R,Jain M

更新日期：2013-08-01 00:00:00

abstract：:Transgenic Bt rice expressing the insecticidal proteins derived from Bacillus thuringiensis Berliner (Bt) has been developed since 1989. Their ecological risks towards non-target organisms have been investigated; however, these studies were conducted individually, yielding uncertainty regarding potential agroecologica...

journal_title：Plant biotechnology journal

authors： Dang C,Lu Z,Wang L,Chang X,Wang F,Yao H,Peng Y,Stanley D,Ye G

更新日期：2017-08-01 00:00:00

abstract：:Threonine (Thr) is one of a few limiting essential amino acids (EAAs) in the animal feed industry, and its level in feed rations can impact production of important meat sources, such as swine and poultry. Threonine as well as EAAs lysine (Lys) and methionine (Met) are all synthesized via the aspartate family pathway. ...

journal_title：Plant biotechnology journal

authors： Qi Q,Huang J,Crowley J,Ruschke L,Goldman BS,Wen L,Rapp WD

更新日期：2011-02-01 00:00:00

abstract：:Downy mildew (Plasmopara halstedii (Farl.) Berlese et de Toni) is a serious foliar pathogen of cultivated sunflower (Helianthus annuus L.). Genetic resistance is conditioned by several linked downy mildew resistance gene specificities in the HaRGC1 cluster of TIR-NBS-LRR resistance gene candidates (RGCs) on linkage gr...

journal_title：Plant biotechnology journal

authors： Slabaugh MB,Yu JK,Tang S,Heesacker A,Hu X,Lu G,Bidney D,Han F,Knapp SJ

更新日期：2003-05-01 00:00:00

abstract：:Grain size, number and starch content are important determinants of grain yield and quality. One of the most important biological processes that determine these components is the carbon partitioning during the early grain filling, which requires the function of cell wall invertase. Here, we showed the constitutive exp...

journal_title：Plant biotechnology journal

authors： Li B,Liu H,Zhang Y,Kang T,Zhang L,Tong J,Xiao L,Zhang H

更新日期：2013-12-01 00:00:00

abstract：:Sumoylation is a posttranslational regulatory process in higher eukaryotes modifying substrate proteins through conjugation of small ubiquitin-related modifiers (SUMOs). Sumoylation modulates protein stability, subcellular localization and activity; thus, it regulates most cellular functions including response to envi...

journal_title：Plant biotechnology journal

authors： Li Z,Hu Q,Zhou M,Vandenbrink J,Li D,Menchyk N,Reighard S,Norris A,Liu H,Sun D,Luo H

更新日期：2013-05-01 00:00:00