Modifying lysine biosynthesis and catabolism in corn with a single bifunctional expression/silencing transgene cassette.

Abstract:

:Although it is one of the major crops in the world, corn has poor nutritional quality for human and animal consumption due to its low lysine content. Here, we report a method of simultaneous expression of a deregulated lysine biosynthetic enzyme, CordapA, and reduction of a bifunctional lysine degradation enzyme, lysine-ketoglutarate reductase/saccharophine dehydrogenase (LKR/SDH), in transgenic corn plants by a single transgene cassette. This is accomplished by inserting an inverted-repeat sequence targeting the maize LKR/SDH gene into an intron of a transgene cassette that expresses CordapA. This combination of LKR/SDH silencing and CordapA expression led to the accumulation of free lysine to over 4000 p.p.m. in transgenic corn grain, compared to less than 100 p.p.m. in wild-type controls. This intron-embedded silencing cassette design reduces the number of transgene cassettes needed in transgenic approaches for manipulating metabolic pathways that sometimes require expression of one gene and silencing of another.

journal_name

Plant Biotechnol J

authors

Frizzi A,Huang S,Gilbertson LA,Armstrong TA,Luethy MH,Malvar TM

doi

10.1111/j.1467-7652.2007.00290.x

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

13-21

issue

1

eissn

1467-7644

issn

1467-7652

pii

PBI290

journal_volume

6

pub_type

杂志文章
  • Constitutive expression of cell wall invertase genes increases grain yield and starch content in maize.

    abstract::Grain size, number and starch content are important determinants of grain yield and quality. One of the most important biological processes that determine these components is the carbon partitioning during the early grain filling, which requires the function of cell wall invertase. Here, we showed the constitutive exp...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12102

    authors: Li B,Liu H,Zhang Y,Kang T,Zhang L,Tong J,Xiao L,Zhang H

    更新日期:2013-12-01 00:00:00

  • Haplotype-based genotyping-by-sequencing in oat genome research.

    abstract::In a de novo genotyping-by-sequencing (GBS) analysis of short, 64-base tag-level haplotypes in 4657 accessions of cultivated oat, we discovered 164741 tag-level (TL) genetic variants containing 241224 SNPs. From this, the marker density of an oat consensus map was increased by the addition of more than 70000 loci. The...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12888

    authors: Bekele WA,Wight CP,Chao S,Howarth CJ,Tinker NA

    更新日期:2018-08-01 00:00:00

  • Freedom-to-operate analysis of a transgenic multivitamin corn variety.

    abstract::In this article, we explore the intellectual property (IP) landscape relevant to the production and commercialization of Carolight(™) , a transgenic multivitamin corn variety created on humanitarian grounds to address micronutrient deficiencies in low-and-middle-income countries. The successful production of this vari...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12488

    authors: Zanga D,Capell T,Zhu C,Christou P,Thangaraj H

    更新日期:2016-05-01 00:00:00

  • Deciphering drought-induced metabolic responses and regulation in developing maize kernels.

    abstract::Drought stress conditions decrease maize growth and yield, and aggravate preharvest aflatoxin contamination. While several studies have been performed on mature kernels responding to drought stress, the metabolic profiles of developing kernels are not as well characterized, particularly in germplasm with contrasting r...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12899

    authors: Yang L,Fountain JC,Ji P,Ni X,Chen S,Lee RD,Kemerait RC,Guo B

    更新日期:2018-02-12 00:00:00

  • The impact of secondary pests on Bacillus thuringiensis (Bt) crops.

    abstract::The intensification of agriculture and the development of synthetic insecticides enabled worldwide grain production to more than double in the last third of the 20th century. However, the heavy dependence and, in some cases, overuse of insecticides has been responsible for negative environmental and ecological impacts...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/pbi.12363

    authors: Catarino R,Ceddia G,Areal FJ,Park J

    更新日期:2015-06-01 00:00:00

  • An AGAMOUS intron-driven cytotoxin leads to flowerless tobacco and produces no detrimental effects on vegetative growth of either tobacco or poplar.

    abstract::Flowerless trait is highly desirable for poplar because it can prevent pollen- and seed-mediated transgene flow. We have isolated the second intron of PTAG2, an AGAMOUS (AG) orthologue from Populus trichocarpa. By fusing this intron sequence to a minimal 35S promoter sequence, we created two artificial promoters, fPTA...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12581

    authors: Li W,Hu W,Fang C,Chen L,Zhuang W,Katin-Grazzini L,McAvoy RJ,Guillard K,Li Y

    更新日期:2016-12-01 00:00:00

  • Enhanced levels of methionine and cysteine in transgenic alfalfa (Medicago sativa L.) plants over-expressing the Arabidopsis cystathionine gamma-synthase gene.

    abstract::With the aim of increasing the methionine level in alfalfa (Medicago sativa L.) and thus improving its nutritional quality, we produced transgenic alfalfa plants that expressed the Arabidopsis cystathionine gamma-synthase (AtCGS), the enzyme that controls the synthesis of the first intermediate metabolite in the methi...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2004.00102.x

    authors: Avraham T,Badani H,Galili S,Amir R

    更新日期:2005-01-01 00:00:00

  • Direct and tunable modulation of protein levels in rice and wheat with a synthetic small molecule.

    abstract::Direct control of protein level enables rapid and efficient analyses of gene functions in crops. Previously, we developed the RDDK-Shield1 (Shld1) system in the model plant Arabidopsis thaliana for direct modulation of protein stabilization using a synthetic small molecule. However, it was unclear whether this system ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12787

    authors: Zhang J,Yin K,Sun J,Gao J,Du Q,Li H,Qiu JL

    更新日期:2018-02-01 00:00:00

  • Engineered selective plant male sterility through pollen-specific expression of the EcoRI restriction endonuclease.

    abstract::Unintended gene flow from transgenic plants via pollen, seed and vegetative propagation is a regulatory concern because of potential admixture in food and crop systems, as well as hybridization and introgression to wild and weedy relatives. Bioconfinement of transgenic pollen would help address some of these concerns ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12493

    authors: Millwood RJ,Moon HS,Poovaiah CR,Muthukumar B,Rice JH,Abercrombie JM,Abercrombie LL,Green WD,Stewart CN Jr

    更新日期:2016-05-01 00:00:00

  • Genomic analyses of diverse wild and cultivated accessions provide insights into the evolutionary history of jujube.

    abstract::The Chinese jujube (Ziziphus jujuba Mill.), a member of the Rhamnaceae family, is an important perennial fruit tree crop of substantial economic, ecological and nutritional value, and is also used as a traditional herbal medicine. Here, we report the resequencing of 493 jujube accessions, including 202 wild and 291 cu...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13480

    authors: Guo M,Zhang Z,Li S,Lian Q,Fu P,He Y,Qiao J,Xu K,Liu L,Wu M,Du Z,Li S,Wang J,Shao P,Yu Q,Xu G,Li D,Wang Y,Tian S,Zhao J,Feng X,Li R,Jiang W,Zhao X

    更新日期:2020-09-18 00:00:00

  • Activity-based proteomics reveals nine target proteases for the recombinant protein-stabilizing inhibitor SlCYS8 in Nicotiana benthamiana.

    abstract::Co-expression of protease inhibitors like the tomato cystatin SlCYS8 is useful to increase recombinant protein production in plants, but key proteases involved in protein proteolysis are still unknown. Here, we performed activity-based protein profiling to identify proteases that are inhibited by SlCYS8 in agroinfiltr...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13092

    authors: Jutras PV,Grosse-Holz F,Kaschani F,Kaiser M,Michaud D,van der Hoorn RAL

    更新日期:2019-08-01 00:00:00

  • CRISPR/Cas9-mediated knockout of six glycosyltransferase genes in Nicotiana benthamiana for the production of recombinant proteins lacking β-1,2-xylose and core α-1,3-fucose.

    abstract::Plants offer fast, flexible and easily scalable alternative platforms for the production of pharmaceutical proteins, but differences between plant and mammalian N-linked glycans, including the presence of β-1,2-xylose and core α-1,3-fucose residues in plants, can affect the activity, potency and immunogenicity of plan...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12981

    authors: Jansing J,Sack M,Augustine SM,Fischer R,Bortesi L

    更新日期:2019-02-01 00:00:00

  • The origins and progress of genomics research on Tef (Eragrostis tef).

    abstract::Tef, Eragrostis tef (Zucc.) Trotter, is the most important cereal in Ethiopia. Tef is cultivated by more than five million small-scale farmers annually and constitutes the staple food for more than half of the population of 80 million. The crop is preferred by both farmers and consumers due to its beneficial traits as...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/pbi.12199

    authors: Girma D,Assefa K,Chanyalew S,Cannarozzi G,Kuhlemeier C,Tadele Z

    更新日期:2014-06-01 00:00:00

  • Genetic spell-checking: gene editing using single-stranded DNA oligonucleotides.

    abstract::Single-stranded oligonucleotides (ssODNs) can be used to direct the exchange of a single nucleotide or the repair of a single base within the coding region of a gene in a process that is known, generically, as gene editing. These molecules are composed of either all DNA residues or a mixture of RNA and DNA bases and u...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/pbi.12473

    authors: Rivera-Torres N,Kmiec EB

    更新日期:2016-02-01 00:00:00

  • ZFN-induced mutagenesis and gene-targeting in Arabidopsis through Agrobacterium-mediated floral dip transformation.

    abstract::Zinc-finger nucleases (ZFNs) are artificial restriction enzymes, custom designed for induction of double-strand breaks (DSBs) at a specific locus. These DSBs may result in site-specific mutagenesis or homologous recombination at the repair site, depending on the DNA repair pathway that is used. These promising techniq...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2009.00446.x

    authors: de Pater S,Neuteboom LW,Pinas JE,Hooykaas PJ,van der Zaal BJ

    更新日期:2009-10-01 00:00:00

  • Metallothionein expression in chloroplasts enhances mercury accumulation and phytoremediation capability.

    abstract::Genetic engineering to enhance mercury phytoremediation has been accomplished by expression of the merAB genes that protects the cell by converting Hg[II] into Hg[0] which volatilizes from the cell. A drawback of this approach is that toxic Hg is released back into the environment. A better phytoremediation strategy w...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2011.00616.x

    authors: Ruiz ON,Alvarez D,Torres C,Roman L,Daniell H

    更新日期:2011-06-01 00:00:00

  • Induction and recovery of copy number variation in banana through gamma irradiation and low-coverage whole-genome sequencing.

    abstract::Traditional breeding methods are hindered in bananas due to the fact that major cultivars are sterile, parthenocarpic, triploid and thus clonally propagated. This has resulted in a narrow genetic base and limited resilience to biotic and abiotic stresses. Mutagenesis of in vitro propagated bananas is one method to int...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12901

    authors: Datta S,Jankowicz-Cieslak J,Nielen S,Ingelbrecht I,Till BJ

    更新日期:2018-02-24 00:00:00

  • CRISPR/dCas9 platforms in plants: strategies and applications beyond genome editing.

    abstract::Clustered regularly interspaced short palindromic repeat (CRISPR) and Cas9-associated protein systems provide a powerful genetic manipulation tool that can drive plant research forward. Nuclease-dead Cas9 (dCas9) is an enzymatically inactive mutant of Cas9 in which its endonuclease activity is non-functional. The appl...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/pbi.13232

    authors: Moradpour M,Abdulah SNA

    更新日期:2020-01-01 00:00:00

  • Application of two bicistronic systems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm.

    abstract::Coordination of multiple transgenes is essential for metabolic engineering of biosynthetic pathways. Here, we report the utilization of two bicistronic systems involving the 2A sequence from the foot-and-mouth disease virus and the internal ribosome entry site (IRES) sequence from the crucifer-infecting tobamovirus to...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2010.00543.x

    authors: Ha SH,Liang YS,Jung H,Ahn MJ,Suh SC,Kweon SJ,Kim DH,Kim YM,Kim JK

    更新日期:2010-10-01 00:00:00

  • A maize phytochrome-interacting factors protein ZmPIF1 enhances drought tolerance by inducing stomatal closure and improves grain yield in Oryza sativa.

    abstract::Phytochrome-interacting factors (PIFs) play major roles in regulating plant growth and development, but their roles in drought stress remain elusive. Here, we cloned and characterized a maize (Zea mays) PIF transcription factor, ZmPIF1. The expression level of ZmPIF1 was significantly induced by independent drought an...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12878

    authors: Gao Y,Wu M,Zhang M,Jiang W,Ren X,Liang E,Zhang D,Zhang C,Xiao N,Li Y,Dai Y,Chen J

    更新日期:2018-07-01 00:00:00

  • Engineering starch accumulation by manipulation of phosphate metabolism of starch.

    abstract::A new understanding of leaf starch degradation has emerged in the last 10 years. It has been shown that starch phosphorylation and dephosphorylation are critical components of this process. Glucan, water dikinase (GWD) (and phosphoglucan, water dikinase) adds phosphate to starch, and phosphoglucan phosphatase (SEX4) r...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2012.00684.x

    authors: Weise SE,Aung K,Jarou ZJ,Mehrshahi P,Li Z,Hardy AC,Carr DJ,Sharkey TD

    更新日期:2012-06-01 00:00:00

  • Heterologous expression of OsSIZ1, a rice SUMO E3 ligase, enhances broad abiotic stress tolerance in transgenic creeping bentgrass.

    abstract::Sumoylation is a posttranslational regulatory process in higher eukaryotes modifying substrate proteins through conjugation of small ubiquitin-related modifiers (SUMOs). Sumoylation modulates protein stability, subcellular localization and activity; thus, it regulates most cellular functions including response to envi...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12030

    authors: Li Z,Hu Q,Zhou M,Vandenbrink J,Li D,Menchyk N,Reighard S,Norris A,Liu H,Sun D,Luo H

    更新日期:2013-05-01 00:00:00

  • Foreign gene products can be enhanced by introduction into low storage protein mutants.

    abstract::Transgenic rice expressing soybean glycinin in its endosperm was crossed with two types of low-glutelin mutants to determine how much storage the protein mutants can contribute to increases in glycinin accumulation. The glycinin level (102 microg/100 mg seed) in the parental transgenic line was enhanced to approximate...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1046/j.1467-7652.2003.00038.x

    authors: Tada Y,Utsumi S,Takaiwa F

    更新日期:2003-11-01 00:00:00

  • Oligonucleotide-directed mutagenesis for precision gene editing.

    abstract::Differences in gene sequences, many of which are single nucleotide polymorphisms, underlie some of the most important traits in plants. With humanity facing significant challenges to increase global agricultural productivity, there is an urgent need to accelerate the development of these traits in plants. oligonucleot...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/pbi.12496

    authors: Sauer NJ,Mozoruk J,Miller RB,Warburg ZJ,Walker KA,Beetham PR,Schöpke CR,Gocal GF

    更新日期:2016-02-01 00:00:00

  • FLP recombinase-mediated site-specific recombination in rice.

    abstract::The feasibility of using the FLP/FRT site-specific recombination system in rice for genome engineering was evaluated. Transgenic rice plants expressing the FLP recombinase were crossed with plants harbouring the kanamycin resistance gene (neomycin phosphotransferase II, nptII) flanked by FRT sites, which also served t...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2007.00310.x

    authors: Hu Q,Kononowicz-Hodges H,Nelson-Vasilchik K,Viola D,Zeng P,Liu H,Kausch AP,Chandlee JM,Hodges TK,Luo H

    更新日期:2008-02-01 00:00:00

  • Stable production of cyanophycinase in Nicotiana benthamiana and its functionality to hydrolyse cyanophycin in the murine intestine.

    abstract::Food supplementation with the conditionally essential amino acid arginine (Arg) has been shown to have nutritional benefits. Degradation of cyanophycin (CGP), a peptide polymer used for nitrogen storage by cyanobacteria, requires cyanophycinase (CGPase) and results in the release of β-aspartic acid (Asp)-Arg dipeptide...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12658

    authors: Ponndorf D,Ehmke S,Walliser B,Thoss K,Unger C,Görs S,Daş G,Metges CC,Broer I,Nausch H

    更新日期:2017-05-01 00:00:00

  • Agrobacterium tumefaciens-mediated sorghum transformation using a mannose selection system.

    abstract::A dual-marker plasmid containing the selectable marker gene, manA, and the reporter gene, sgfp, was used to transform immature sorghum embryos by employing an Agrobacterium-mediated system. Both genes were under the control of the ubi1 promoter in a binary vector pPZP201. The Escherichia coli phosphomannose isomerase ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2005.00150.x

    authors: Gao Z,Xie X,Ling Y,Muthukrishnan S,Liang GH

    更新日期:2005-11-01 00:00:00

  • Immunotherapy using algal-produced Ara h 1 core domain suppresses peanut allergy in mice.

    abstract::Peanut allergy is an IgE-mediated adverse reaction to a subset of proteins found in peanuts. Immunotherapy aims to desensitize allergic patients through repeated and escalating exposures for several months to years using extracts or flours. The complex mix of proteins and variability between preparations complicates i...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12515

    authors: Gregory JA,Shepley-McTaggart A,Umpierrez M,Hurlburt BK,Maleki SJ,Sampson HA,Mayfield SP,Berin MC

    更新日期:2016-07-01 00:00:00

  • The TaDREB3 transgene transferred by conventional crossings to different genetic backgrounds of bread wheat improves drought tolerance.

    abstract::Drought tolerance of the wheat cultivar Bobwhite was previously enhanced by transformation with a construct containing the wheat DREB3 gene driven by the stress-inducible maize Rab17 promoter. Progeny of a single T2 transgenic line were used as pollinators in crosses with four elite bread wheat cultivars from Western ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12385

    authors: Shavrukov Y,Baho M,Lopato S,Langridge P

    更新日期:2016-01-01 00:00:00

  • Commercialization of new biotechnology: a systematic review of 16 commercial case studies in a novel manufacturing sector.

    abstract::The 1980s and 1990s saw a major expansion of biotechnology into new areas of science including genomics and recombinant technologies. This was coupled to the widespread emergence of academics into the commercial sector as they were encouraged to spin out companies or commercialize their intellectual property. There we...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/pbi.12426

    authors: Paul MJ,Thangaraj H,Ma JK

    更新日期:2015-10-01 00:00:00