Abstract:
:The plant immune system involves detection of pathogens via both cell-surface and intracellular receptors. Both receptor classes can induce transcriptional reprogramming that elevates disease resistance. To assess differential gene expression during plant immunity, we developed and deployed quantitative sequence capture (CAP-I). We designed and synthesized biotinylated single-strand RNA bait libraries targeted to a subset of defense genes, and generated sequence capture data from 99 RNA-seq libraries. We built a data processing pipeline to quantify the RNA-CAP-I-seq data, and visualize differential gene expression. Sequence capture in combination with quantitative RNA-seq enabled cost-effective assessment of the expression profile of a specified subset of genes. Quantitative sequence capture is not limited to RNA-seq or any specific organism and can potentially be incorporated into automated platforms for high-throughput sequencing.
journal_name
Plant Biotechnol Jjournal_title
Plant biotechnology journalauthors
Ding P,Ngou BPM,Furzer OJ,Sakai T,Shrestha RK,MacLean D,Jones JDGdoi
10.1111/pbi.13327subject
Has Abstractpub_date
2020-07-01 00:00:00pages
1610-1619issue
7eissn
1467-7644issn
1467-7652journal_volume
18pub_type
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