Abstract:
:The capacity of the bread wheat (Triticum aestivum) genome to tolerate introgression from related genomes can be exploited for wheat improvement. A resistance to powdery mildew expressed by a derivative of the cross-bread wheat cv. Tähti × T. militinae (Tm) is known to be due to the incorporation of a Tm segment into the long arm of chromosome 4A. Here, a newly developed in silico method termed rearrangement identification and characterization (RICh) has been applied to characterize the introgression. A virtual gene order, assembled using the GenomeZipper approach, was obtained for the native copy of chromosome 4A; it incorporated 570 4A DArTseq markers to produce a zipper comprising 2132 loci. A comparison between the native and introgressed forms of the 4AL chromosome arm showed that the introgressed region is located at the distal part of the arm. The Tm segment, derived from chromosome 7G, harbours 131 homoeologs of the 357 genes present on the corresponding region of Chinese Spring 4AL. The estimated number of Tm genes transferred along with the disease resistance gene was 169. Characterizing the introgression's position, gene content and internal gene order should not only facilitate gene isolation, but may also be informative with respect to chromatin structure and behaviour studies.
journal_name
Plant Biotechnol Jjournal_title
Plant biotechnology journalauthors
Abrouk M,Balcárková B,Šimková H,Komínkova E,Martis MM,Jakobson I,Timofejeva L,Rey E,Vrána J,Kilian A,Järve K,Doležel J,Valárik Mdoi
10.1111/pbi.12610subject
Has Abstractpub_date
2017-02-01 00:00:00pages
249-256issue
2eissn
1467-7644issn
1467-7652journal_volume
15pub_type
杂志文章abstract::Transgenic engineering of plants is important in both basic and applied research. However, the expression of a transgene can dwindle over time as the plant's small (s)RNA-guided silencing pathways shut it down. The silencing pathways have evolved as antiviral defence mechanisms, and viruses have co-evolved viral silen...
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更新日期:2015-08-01 00:00:00
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journal_title:Plant biotechnology journal
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更新日期:2017-05-01 00:00:00
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journal_title:Plant biotechnology journal
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