The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants.

abstract：:Advances in the genetic transformation of cereals have improved the prospects of using biotechnology for plant improvement, and a toolbox of promoters with defined specificities would be a valuable resource in controlling the expression of transgenes in desired tissues for both plant improvement and molecular farming....

journal_title：Plant biotechnology journal

authors： Furtado A,Henry RJ,Pellegrineschi A

更新日期：2009-04-01 00:00:00

abstract：:Drought tolerance of the wheat cultivar Bobwhite was previously enhanced by transformation with a construct containing the wheat DREB3 gene driven by the stress-inducible maize Rab17 promoter. Progeny of a single T2 transgenic line were used as pollinators in crosses with four elite bread wheat cultivars from Western ...

journal_title：Plant biotechnology journal

authors： Shavrukov Y,Baho M,Lopato S,Langridge P

更新日期：2016-01-01 00:00:00

abstract：:Transgenic rice accumulating the modified major Japanese cedar pollen allergens, Cryptomeria japonica 1 (Cry j 1) and Cryptomeria japonica 2 (Cry j 2), which were deconstructed by fragmentation and shuffling, respectively, in the edible part of the seed was generated by transformation of a good-tasting rice variety, '...

journal_title：Plant biotechnology journal

authors： Wakasa Y,Takagi H,Hirose S,Yang L,Saeki M,Nishimura T,Kaminuma O,Hiroi T,Takaiwa F

更新日期：2013-01-01 00:00:00

abstract：:Triterpenes are thirty-carbon compounds derived from the universal five-carbon prenyl precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Normally, triterpenes are synthesized via the mevalonate (MVA) pathway operating in the cytoplasm of eukaryotes where DMAPP is condensed with two IPPs to...

journal_title：Plant biotechnology journal

authors： Kempinski C,Jiang Z,Zinck G,Sato SJ,Ge Z,Clemente TE,Chappell J

更新日期：2019-02-01 00:00:00

abstract：:Ethylene response factor 1 (ERF1) is an essential integrator of the jasmonate and ethylene signalling pathways coordinating a large number of genes involved in plant defences. Its orthologue in Hevea brasiliensis, HbERF-IXc5, has been assumed to play a major role in laticifer metabolism and tolerance to harvesting str...

journal_title：Plant biotechnology journal

authors： Lestari R,Rio M,Martin F,Leclercq J,Woraathasin N,Roques S,Dessailly F,Clément-Vidal A,Sanier C,Fabre D,Melliti S,Suharsono S,Montoro P

更新日期：2018-01-01 00:00:00

abstract：:Measurement of gene expression can provide important clues about gene function and molecular basis of developmental processes. Here, we have analysed the chickpea transcriptome in vegetative and flower tissues by exploiting the potential of high-throughput sequencing to measure gene expression. We mapped more than 295...

journal_title：Plant biotechnology journal

authors： Singh VK,Garg R,Jain M

更新日期：2013-08-01 00:00:00

abstract：:There is a pressing need to develop novel antibacterial agents given the widespread antibiotic resistance among pathogenic bacteria and the low specificity of the drugs available. Endolysins are antibacterial proteins that are produced by bacteriophage-infected cells to digest the bacterial cell wall for phage progeny...

journal_title：Plant biotechnology journal

authors： Stoffels L,Taunt HN,Charalambous B,Purton S

更新日期：2017-09-01 00:00:00

abstract：:Pollen germination and pollen tube growth are important physiological processes of sexual reproduction of plants and also are involved in signal transduction. Our previous study reveals that ZmSTK1 and ZmSTK2 are two receptor-like cytoplasmic kinases (RLCK) homologs in Zea mays as members of receptor-like protein kina...

journal_title：Plant biotechnology journal

authors： Fan M,Zhang C,Shi L,Liu C,Ma W,Chen M,Liu K,Cai F,Wang G,Wei Z,Jiang M,Liu Z,Javeed A,Lin F

更新日期：2018-08-01 00:00:00

abstract：:A high-amylose rice with 64.8% amylose content (AC) was developed by transgenic inhibition of two isoforms of starch branching enzyme (SBE), SBEI and SBEIIb, in an indica rice cultivar. The expression of SBEI and SBEIIb was completely inhibited in the transgenic line, whereas the expression of granule-bound starch syn...

journal_title：Plant biotechnology journal

authors： Zhu L,Gu M,Meng X,Cheung SC,Yu H,Huang J,Sun Y,Shi Y,Liu Q

更新日期：2012-04-01 00:00:00

abstract：:Phytochrome-interacting factors (PIFs) play major roles in regulating plant growth and development, but their roles in drought stress remain elusive. Here, we cloned and characterized a maize (Zea mays) PIF transcription factor, ZmPIF1. The expression level of ZmPIF1 was significantly induced by independent drought an...

journal_title：Plant biotechnology journal

authors： Gao Y,Wu M,Zhang M,Jiang W,Ren X,Liang E,Zhang D,Zhang C,Xiao N,Li Y,Dai Y,Chen J

更新日期：2018-07-01 00:00:00

abstract：:DNA recombination reactions (site-specific and homologous) were monitored in the progeny of transgenic maize plants by bringing together two recombination substrates (docking sites and shuttle vectors) in the zygotes. In one combination of transgenic events, the recombination marker gene (yellow fluorescent protein ge...

journal_title：Plant biotechnology journal

authors： Djukanovic V,Orczyk W,Gao H,Sun X,Garrett N,Zhen S,Gordon-Kamm W,Barton J,Lyznik LA

更新日期：2006-05-01 00:00:00

abstract：:Unintended gene flow from transgenic plants via pollen, seed and vegetative propagation is a regulatory concern because of potential admixture in food and crop systems, as well as hybridization and introgression to wild and weedy relatives. Bioconfinement of transgenic pollen would help address some of these concerns ...

journal_title：Plant biotechnology journal

authors： Millwood RJ,Moon HS,Poovaiah CR,Muthukumar B,Rice JH,Abercrombie JM,Abercrombie LL,Green WD,Stewart CN Jr

更新日期：2016-05-01 00:00:00

abstract：:Zinc-finger nucleases (ZFNs) are artificial restriction enzymes, custom designed for induction of double-strand breaks (DSBs) at a specific locus. These DSBs may result in site-specific mutagenesis or homologous recombination at the repair site, depending on the DNA repair pathway that is used. These promising techniq...

journal_title：Plant biotechnology journal

authors： de Pater S,Neuteboom LW,Pinas JE,Hooykaas PJ,van der Zaal BJ

更新日期：2009-10-01 00:00:00

abstract：:In a de novo genotyping-by-sequencing (GBS) analysis of short, 64-base tag-level haplotypes in 4657 accessions of cultivated oat, we discovered 164741 tag-level (TL) genetic variants containing 241224 SNPs. From this, the marker density of an oat consensus map was increased by the addition of more than 70000 loci. The...

journal_title：Plant biotechnology journal

authors： Bekele WA,Wight CP,Chao S,Howarth CJ,Tinker NA

更新日期：2018-08-01 00:00:00

abstract：:In this article, we explore the intellectual property (IP) landscape relevant to the production and commercialization of Carolight(™) , a transgenic multivitamin corn variety created on humanitarian grounds to address micronutrient deficiencies in low-and-middle-income countries. The successful production of this vari...

journal_title：Plant biotechnology journal

authors： Zanga D,Capell T,Zhu C,Christou P,Thangaraj H

更新日期：2016-05-01 00:00:00

abstract：:GM crops are the most studied crops in history. Approximately 5% of the safety studies on them show adverse effects that are a cause for concern and tend to be featured in media reports. Although these reports are based on just a handful of GM events, they are used to cast doubt on all GM crops. Furthermore, they tend...

journal_title：Plant biotechnology journal

authors： Sánchez MA,Parrott WA

更新日期：2017-10-01 00:00:00

abstract：:Cadmium (Cd) is a widespread soil contaminant threatening human health. As an ideal energy plant, sweet sorghum (Sorghum bicolor (L.) Moench) has great potential in phytoremediation of Cd-polluted soils, although the molecular mechanisms are largely unknown. In this study, key factors responsible for differential Cd a...

journal_title：Plant biotechnology journal

authors： Feng J,Jia W,Lv S,Bao H,Miao F,Zhang X,Wang J,Li J,Li D,Zhu C,Li S,Li Y

更新日期：2018-02-01 00:00:00

abstract：:Virus-induced gene silencing (VIGS) is a powerful tool for functional analysis of genes in plants. A wide-host-range VIGS vector, which was developed based on the Cucumber mosaic virus (CMV), was tested for its ability to silence endogenous genes involved in flavonoid biosynthesis in soybean. Symptomless infection was...

journal_title：Plant biotechnology journal

authors： Nagamatsu A,Masuta C,Senda M,Matsuura H,Kasai A,Hong JS,Kitamura K,Abe J,Kanazawa A

更新日期：2007-11-01 00:00:00

abstract：:Differences in gene sequences, many of which are single nucleotide polymorphisms, underlie some of the most important traits in plants. With humanity facing significant challenges to increase global agricultural productivity, there is an urgent need to accelerate the development of these traits in plants. oligonucleot...

journal_title：Plant biotechnology journal

authors： Sauer NJ,Mozoruk J,Miller RB,Warburg ZJ,Walker KA,Beetham PR,Schöpke CR,Gocal GF

更新日期：2016-02-01 00:00:00

abstract：:The development and adoption of hybrid seed technology have led to dramatic increases in agricultural productivity. However, it has been a challenge to develop a commercially viable platform for the production of hybrid wheat (Triticum aestivum) seed due to wheat's strong inbreeding habit. Recently, a novel platform f...

journal_title：Plant biotechnology journal

authors： Okada A,Arndell T,Borisjuk N,Sharma N,Watson-Haigh NS,Tucker EJ,Baumann U,Langridge P,Whitford R

更新日期：2019-10-01 00:00:00

abstract：:Genome modification by homology-directed repair (HDR) is an attractive tool for the controlled genetic manipulation of plants. Here, we report the HDR-mediated gene exchange of expression cassettes in tobacco BY-2 cells using a designed zinc finger nuclease (ZFN). The target contained a 7-kb fragment flanked by two ZF...

journal_title：Plant biotechnology journal

authors： Schneider K,Schiermeyer A,Dolls A,Koch N,Herwartz D,Kirchhoff J,Fischer R,Russell SM,Cao Z,Corbin DR,Sastry-Dent L,Ainley WM,Webb SR,Schinkel H,Schillberg S

更新日期：2016-04-01 00:00:00

abstract：:The tissue-specific expression of transgenes is essential in plant breeding programmes to avoid the fitness costs caused by constitutive expression of a target gene. However, knowledge on the molecular mechanisms of tissue-specific gene expression and practicable tissue-specific promoters is limited. In this study, we...

journal_title：Plant biotechnology journal

authors： Cai M,Wei J,Li X,Xu C,Wang S

更新日期：2007-09-01 00:00:00

abstract：:Antioxidants are protective molecules that neutralize reactive oxygen species and prevent oxidative damage to cellular components such as membranes, proteins and nucleic acids, therefore reducing the rate of cell death and hence the effects of ageing and ageing-related diseases. The fortification of food with antioxid...

journal_title：Plant biotechnology journal

authors： Zhu C,Sanahuja G,Yuan D,Farré G,Arjó G,Berman J,Zorrilla-López U,Banakar R,Bai C,Pérez-Massot E,Bassie L,Capell T,Christou P

更新日期：2013-02-01 00:00:00

abstract：:The intensification of agriculture and the development of synthetic insecticides enabled worldwide grain production to more than double in the last third of the 20th century. However, the heavy dependence and, in some cases, overuse of insecticides has been responsible for negative environmental and ecological impacts...

journal_title：Plant biotechnology journal

authors： Catarino R,Ceddia G,Areal FJ,Park J

更新日期：2015-06-01 00:00:00

abstract：:Genetic improvement of fibre quality is one of the main breeding goals for the upland cotton, Gossypium hirsutum, but there are difficulties with precise selection of traits. Therefore, it is important to improve the understanding of the genetic basis of phenotypic variation. In this study, we conducted phenotyping an...

journal_title：Plant biotechnology journal

authors： Sun Z,Wang X,Liu Z,Gu Q,Zhang Y,Li Z,Ke H,Yang J,Wu J,Wu L,Zhang G,Zhang C,Ma Z

更新日期：2017-08-01 00:00:00

abstract：:Dengue fever is a mosquito (Aedes aegypti) -transmitted viral disease that is endemic in more than 125 countries around the world. There are four serotypes of the dengue virus (DENV 1-4) and a safe and effective dengue vaccine must provide protection against all four serotypes. To date, the first vaccine, Dengvaxia (C...

journal_title：Plant biotechnology journal

authors： van Eerde A,Gottschamel J,Bock R,Hansen KEA,Munang'andu HM,Daniell H,Liu Clarke J

更新日期：2019-07-01 00:00:00

abstract：:In the past few years, the usefulness of transient expression assays has continuously increased for the characterization of unknown gene function and metabolic pathways. In grapevine (Vitis vinifera L.), one of the most economically important fruit crops in the world, recent systematic sequencing projects produced man...

journal_title：Plant biotechnology journal

authors： Jelly NS,Valat L,Walter B,Maillot P

更新日期：2014-12-01 00:00:00

abstract：:Transgenic engineering of plants is important in both basic and applied research. However, the expression of a transgene can dwindle over time as the plant's small (s)RNA-guided silencing pathways shut it down. The silencing pathways have evolved as antiviral defence mechanisms, and viruses have co-evolved viral silen...

journal_title：Plant biotechnology journal

authors： Naim F,Shrestha P,Singh SP,Waterhouse PM,Wood CC

更新日期：2016-06-01 00:00:00

abstract：:The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid...

journal_title：Plant biotechnology journal

authors： Tomlinson L,Yang Y,Emenecker R,Smoker M,Taylor J,Perkins S,Smith J,MacLean D,Olszewski NE,Jones JDG

更新日期：2019-01-01 00:00:00

abstract：:Drought is the major environmental factor limiting crop productivity worldwide. We hypothesized that it is possible to enhance drought tolerance by delaying stress-induced senescence through the stress-induced synthesis of cytokinins in crop-plants. We generated transgenic rice (Oryza sativa) plants expressing an isop...

journal_title：Plant biotechnology journal

authors： Peleg Z,Reguera M,Tumimbang E,Walia H,Blumwald E

更新日期：2011-09-01 00:00:00