Developing xylem-preferential expression of PdGA20ox1, a gibberellin 20-oxidase 1 from Pinus densiflora, improves woody biomass production in a hybrid poplar.

Abstract:

:Woody biomass has gained popularity as an environmentally friendly, renewable and sustainable resource for liquid fuel production. Here, we demonstrate biotechnological improvement of the quantity and quality of woody biomass by employing developing xylem (DX)-preferential production of gibberellin (GA), a phytohormone that positively regulates stem growth. First, for the proof of concept experiment, we produced transgenic Arabidopsis plants expressing GA20-oxidase, a key enzyme in the production of bioactive GAs, from Pinus densiflora (PdGA20ox1) under the control of either a constitutive 35S promoter, designated 35S::PdGA20ox1, or a DX-specific promoter (originated from poplar), designated DX15::PdGA20ox1. As we hypothesized, both transgenic Arabidopsis plants (35S::PdGA20ox1 and DX15::PdGA20ox1) exhibited an accelerated stem growth that resulted in a large increase of biomass, up to 300% compared to wild-type control plants, together with increased secondary wall thickening and elongation of fibre cells. Next, we applied our concept to the production of transgenic poplar trees. Both transgenic poplar trees (35S::PdGA20ox1 and DX15::PdGA20ox1) showed dramatic increases in biomass, up to 300%, with accelerated stem growth and xylem differentiation. Cell wall monosaccharide composition analysis revealed that in both Arabidopsis and poplar, glucose and xylose contents were significantly increased. However, undesirable phenotypes of 35S::PdGA20ox1 poplar, including poor root growth and leaf development, were found. Interestingly, DX15::PdGA20ox1 poplar resulted in a reduction of undesirable phenotypes. Our results indicate that the controlled production of GAs through a tissue-specific promoter can be utilized as an efficient biotechnological tool for producing enhanced plant biomass, minimizing unwanted effects.

journal_name

Plant Biotechnol J

authors

Jeon HW,Cho JS,Park EJ,Han KH,Choi YI,Ko JH

doi

10.1111/pbi.12484

subject

Has Abstract

pub_date

2016-04-01 00:00:00

pages

1161-70

issue

4

eissn

1467-7644

issn

1467-7652

journal_volume

14

pub_type

杂志文章
  • Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase.

    abstract::Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer c...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12359

    authors: Ramírez-Estrada K,Altabella T,Onrubia M,Moyano E,Notredame C,Osuna L,Vanden Bossche R,Goossens A,Cusido RM,Palazon J

    更新日期:2016-01-01 00:00:00

  • ZFN-induced mutagenesis and gene-targeting in Arabidopsis through Agrobacterium-mediated floral dip transformation.

    abstract::Zinc-finger nucleases (ZFNs) are artificial restriction enzymes, custom designed for induction of double-strand breaks (DSBs) at a specific locus. These DSBs may result in site-specific mutagenesis or homologous recombination at the repair site, depending on the DNA repair pathway that is used. These promising techniq...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2009.00446.x

    authors: de Pater S,Neuteboom LW,Pinas JE,Hooykaas PJ,van der Zaal BJ

    更新日期:2009-10-01 00:00:00

  • Sequencing of Australian wild rice genomes reveals ancestral relationships with domesticated rice.

    abstract::The related A genome species of the Oryza genus are the effective gene pool for rice. Here, we report draft genomes for two Australian wild A genome taxa: O. rufipogon-like population, referred to as Taxon A, and O. meridionalis-like population, referred to as Taxon B. These two taxa were sequenced and assembled by in...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12674

    authors: Brozynska M,Copetti D,Furtado A,Wing RA,Crayn D,Fox G,Ishikawa R,Henry RJ

    更新日期:2017-06-01 00:00:00

  • The Lr34 adult plant rust resistance gene provides seedling resistance in durum wheat without senescence.

    abstract::The hexaploid wheat (Triticum aestivum) adult plant resistance gene, Lr34/Yr18/Sr57/Pm38/Ltn1, provides broad-spectrum resistance to wheat leaf rust (Lr34), stripe rust (Yr18), stem rust (Sr57) and powdery mildew (Pm38) pathogens, and has remained effective in wheat crops for many decades. The partial resistance provi...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12684

    authors: Rinaldo A,Gilbert B,Boni R,Krattinger SG,Singh D,Park RF,Lagudah E,Ayliffe M

    更新日期:2017-07-01 00:00:00

  • Generation of marker-free transgenic hexaploid wheat via an Agrobacterium-mediated co-transformation strategy in commercial Chinese wheat varieties.

    abstract::Genotype specificity is a big problem lagging the development of efficient hexaploid wheat transformation system. Increasingly, the biosecurity of genetically modified organisms is garnering public attention, so the generation of marker-free transgenic plants is very important to the eventual potential commercial rele...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12660

    authors: Wang K,Liu H,Du L,Ye X

    更新日期:2017-05-01 00:00:00

  • Silencing the expression of the salivary sheath protein causes transgenerational feeding suppression in the aphid Sitobion avenae.

    abstract::Aphids produce gel saliva during feeding which forms a sheath around the stylet as it penetrates through the apoplast. The sheath is required for the sustained ingestion of phloem sap from sieve elements and is thought to form when the structural sheath protein (SHP) is cross-linked by intermolecular disulphide bridge...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12322

    authors: Abdellatef E,Will T,Koch A,Imani J,Vilcinskas A,Kogel KH

    更新日期:2015-08-01 00:00:00

  • Divergent improvement of two cultivated allotetraploid cotton species.

    abstract::Interspecific genomic variation can provide a genetic basis for local adaptation and domestication. A series of studies have presented its role of interspecific haplotypes and introgressions in adaptive traits, but few studies have addressed their role in improving agronomic character. Two allotetraploid Gossypium spe...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13547

    authors: Fang L,Zhao T,Hu Y,Si Z,Zhu X,Han Z,Liu G,Wang S,Ju L,Guo M,Mei H,Wang L,Qi B,Wang H,Guan X,Zhang T

    更新日期:2021-01-14 00:00:00

  • Whole-genome re-sequencing reveals the impact of the interaction of copy number variants of the rhg1 and Rhg4 genes on broad-based resistance to soybean cyst nematode.

    abstract::Soybean cyst nematode (SCN) is the most devastating plant-parasitic nematode. Most commercial soybean varieties with SCN resistance are derived from PI88788. Resistance derived from PI88788 is breaking down due to narrow genetic background and SCN population shift. PI88788 requires mainly the rhg1-b locus, while 'Peki...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13086

    authors: Patil GB,Lakhssassi N,Wan J,Song L,Zhou Z,Klepadlo M,Vuong TD,Stec AO,Kahil SS,Colantonio V,Valliyodan B,Rice JH,Piya S,Hewezi T,Stupar RM,Meksem K,Nguyen HT

    更新日期:2019-08-01 00:00:00

  • Engineered selective plant male sterility through pollen-specific expression of the EcoRI restriction endonuclease.

    abstract::Unintended gene flow from transgenic plants via pollen, seed and vegetative propagation is a regulatory concern because of potential admixture in food and crop systems, as well as hybridization and introgression to wild and weedy relatives. Bioconfinement of transgenic pollen would help address some of these concerns ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12493

    authors: Millwood RJ,Moon HS,Poovaiah CR,Muthukumar B,Rice JH,Abercrombie JM,Abercrombie LL,Green WD,Stewart CN Jr

    更新日期:2016-05-01 00:00:00

  • Overexpression of SrUGT76G1 in Stevia alters major steviol glycosides composition towards improved quality.

    abstract::Steviol glycosides (SGs) are extracted from Stevia leaves for use as a natural sweetener. Among SGs, stevioside is most abundant in leaf extracts followed by rebaudioside A (Reb A). However, Reb A is of particular interest because of its sweeter and more pleasant taste compared to stevioside. Therefore, the developmen...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13035

    authors: Kim MJ,Zheng J,Liao MH,Jang IC

    更新日期:2019-06-01 00:00:00

  • Using CRISPR/Cas9 genome editing in tomato to create a gibberellin-responsive dominant dwarf DELLA allele.

    abstract::The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12952

    authors: Tomlinson L,Yang Y,Emenecker R,Smoker M,Taylor J,Perkins S,Smith J,MacLean D,Olszewski NE,Jones JDG

    更新日期:2019-01-01 00:00:00

  • Transient expression assays in grapevine: a step towards genetic improvement.

    abstract::In the past few years, the usefulness of transient expression assays has continuously increased for the characterization of unknown gene function and metabolic pathways. In grapevine (Vitis vinifera L.), one of the most economically important fruit crops in the world, recent systematic sequencing projects produced man...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/pbi.12294

    authors: Jelly NS,Valat L,Walter B,Maillot P

    更新日期:2014-12-01 00:00:00

  • Activity-based proteomics reveals nine target proteases for the recombinant protein-stabilizing inhibitor SlCYS8 in Nicotiana benthamiana.

    abstract::Co-expression of protease inhibitors like the tomato cystatin SlCYS8 is useful to increase recombinant protein production in plants, but key proteases involved in protein proteolysis are still unknown. Here, we performed activity-based protein profiling to identify proteases that are inhibited by SlCYS8 in agroinfiltr...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13092

    authors: Jutras PV,Grosse-Holz F,Kaschani F,Kaiser M,Michaud D,van der Hoorn RAL

    更新日期:2019-08-01 00:00:00

  • CRISPR/dCas9 platforms in plants: strategies and applications beyond genome editing.

    abstract::Clustered regularly interspaced short palindromic repeat (CRISPR) and Cas9-associated protein systems provide a powerful genetic manipulation tool that can drive plant research forward. Nuclease-dead Cas9 (dCas9) is an enzymatically inactive mutant of Cas9 in which its endonuclease activity is non-functional. The appl...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/pbi.13232

    authors: Moradpour M,Abdulah SNA

    更新日期:2020-01-01 00:00:00

  • Genome-wide association study discovered genetic variation and candidate genes of fibre quality traits in Gossypium hirsutum L.

    abstract::Genetic improvement of fibre quality is one of the main breeding goals for the upland cotton, Gossypium hirsutum, but there are difficulties with precise selection of traits. Therefore, it is important to improve the understanding of the genetic basis of phenotypic variation. In this study, we conducted phenotyping an...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12693

    authors: Sun Z,Wang X,Liu Z,Gu Q,Zhang Y,Li Z,Ke H,Yang J,Wu J,Wu L,Zhang G,Zhang C,Ma Z

    更新日期:2017-08-01 00:00:00

  • Highly rearranged and size-variable chloroplast genomes in conifers II clade (cupressophytes): evolution towards shorter intergenic spacers.

    abstract::Although conifers are of immense ecological and economic value, bioengineering of their chloroplasts remains undeveloped. Understanding the chloroplast genomic organization of conifers can facilitate their bioengineering. Members of the conifer II clade (or cupressophytes) are highly diverse in both morphologic featur...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12141

    authors: Wu CS,Chaw SM

    更新日期:2014-04-01 00:00:00

  • A maize phytochrome-interacting factors protein ZmPIF1 enhances drought tolerance by inducing stomatal closure and improves grain yield in Oryza sativa.

    abstract::Phytochrome-interacting factors (PIFs) play major roles in regulating plant growth and development, but their roles in drought stress remain elusive. Here, we cloned and characterized a maize (Zea mays) PIF transcription factor, ZmPIF1. The expression level of ZmPIF1 was significantly induced by independent drought an...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12878

    authors: Gao Y,Wu M,Zhang M,Jiang W,Ren X,Liang E,Zhang D,Zhang C,Xiao N,Li Y,Dai Y,Chen J

    更新日期:2018-07-01 00:00:00

  • Up-regulation of an N-terminal truncated 3-hydroxy-3-methylglutaryl CoA reductase enhances production of essential oils and sterols in transgenic Lavandula latifolia.

    abstract::Spike lavender (Lavandula latifolia) essential oil is widely used in the perfume, cosmetic, flavouring and pharmaceutical industries. Thus, modifications of yield and composition of this essential oil by genetic engineering should have important scientific and commercial applications. We generated transgenic spike lav...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2007.00286.x

    authors: Muñoz-Bertomeu J,Sales E,Ros R,Arrillaga I,Segura J

    更新日期:2007-11-01 00:00:00

  • The genetic architecture of the dynamic changes in grain moisture in maize.

    abstract::Low grain moisture at harvest is crucial for safe production, transport, and storage, but the genetic architecture of this trait in maize (Zea mays) remains elusive. Here, we measured the dynamic changes in grain moisture content in an association mapping panel of 513 diverse maize inbred lines at five successive stag...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13541

    authors: Li W,Yu Y,Wang L,Luo Y,Peng Y,Xu Y,Liu X,Wu S,Jian L,Xu J,Xiao Y,Yan J

    更新日期:2021-01-01 00:00:00

  • A global view of transcriptome dynamics during flower development in chickpea by deep sequencing.

    abstract::Measurement of gene expression can provide important clues about gene function and molecular basis of developmental processes. Here, we have analysed the chickpea transcriptome in vegetative and flower tissues by exploiting the potential of high-throughput sequencing to measure gene expression. We mapped more than 295...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12059

    authors: Singh VK,Garg R,Jain M

    更新日期:2013-08-01 00:00:00

  • Biofortification of plants with altered antioxidant content and composition: genetic engineering strategies.

    abstract::Antioxidants are protective molecules that neutralize reactive oxygen species and prevent oxidative damage to cellular components such as membranes, proteins and nucleic acids, therefore reducing the rate of cell death and hence the effects of ageing and ageing-related diseases. The fortification of food with antioxid...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/j.1467-7652.2012.00740.x

    authors: Zhu C,Sanahuja G,Yuan D,Farré G,Arjó G,Berman J,Zorrilla-López U,Banakar R,Bai C,Pérez-Massot E,Bassie L,Capell T,Christou P

    更新日期:2013-02-01 00:00:00

  • Characterization of disease resistance genes in the Brassica napus pangenome reveals significant structural variation.

    abstract::Methods based on single nucleotide polymorphism (SNP), copy number variation (CNV) and presence/absence variation (PAV) discovery provide a valuable resource to study gene structure and evolution. However, as a result of these structural variations, a single reference genome is unable to cover the entire gene content ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13262

    authors: Dolatabadian A,Bayer PE,Tirnaz S,Hurgobin B,Edwards D,Batley J

    更新日期:2020-04-01 00:00:00

  • Rice endosperm is cost-effective for the production of recombinant griffithsin with potent activity against HIV.

    abstract::Protein microbicides containing neutralizing antibodies and antiviral lectins may help to reduce the rate of infection with human immunodeficiency virus (HIV) if it is possible to manufacture the components in large quantities at a cost affordable in HIV-endemic regions such as sub-Saharan Africa. We expressed the ant...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12507

    authors: Vamvaka E,Arcalis E,Ramessar K,Evans A,O'Keefe BR,Shattock RJ,Medina V,Stöger E,Christou P,Capell T

    更新日期:2016-06-01 00:00:00

  • Fusion of an Fc chain to a VHH boosts the accumulation levels in Arabidopsis seeds.

    abstract::Nanobodies® (VHHs) provide powerful tools in therapeutic and biotechnological applications. Nevertheless, for some applications, bivalent antibodies perform much better, and for this, an Fc chain can be fused to the VHH domain, resulting in a bivalent homodimeric VHH-Fc complex. However, the production of bivalent ant...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12094

    authors: De Buck S,Nolf J,De Meyer T,Virdi V,De Wilde K,Van Lerberge E,Van Droogenbroeck B,Depicker A

    更新日期:2013-10-01 00:00:00

  • Whole-genome resequencing-based QTL-seq identified candidate genes and molecular markers for fresh seed dormancy in groundnut.

    abstract::The subspecies fastigiata of cultivated groundnut lost fresh seed dormancy (FSD) during domestication and human-made selection. Groundnut varieties lacking FSD experience precocious seed germination during harvest imposing severe losses. Development of easy-to-use genetic markers enables early-generation selection in ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13266

    authors: Kumar R,Janila P,Vishwakarma MK,Khan AW,Manohar SS,Gangurde SS,Variath MT,Shasidhar Y,Pandey MK,Varshney RK

    更新日期:2020-04-01 00:00:00

  • Next-generation sequencing identified genomic region and diagnostic markers for resistance to bacterial wilt on chromosome B02 in peanut (Arachis hypogaea L.).

    abstract::Bacterial wilt, caused by Ralstonia solanacearum, is a devastating disease affecting over 350 plant species. A few peanut cultivars were found to possess stable and durable bacterial wilt resistance (BWR). Genomics-assisted breeding can accelerate the process of developing resistant cultivars by using diagnostic marke...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13153

    authors: Luo H,Pandey MK,Khan AW,Wu B,Guo J,Ren X,Zhou X,Chen Y,Chen W,Huang L,Liu N,Lei Y,Liao B,Varshney RK,Jiang H

    更新日期:2019-12-01 00:00:00

  • Comparative transcriptomics as a tool for the identification of root branching genes in maize.

    abstract::The root system is fundamental for plant development, is crucial for overall plant growth and is recently being recognized as the key for future crop productivity improvement. A major determinant of root system architecture is the initiation of lateral roots. While knowledge of the genetic and molecular mechanisms reg...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12104

    authors: Jansen L,Hollunder J,Roberts I,Forestan C,Fonteyne P,Van Quickenborne C,Zhen RG,McKersie B,Parizot B,Beeckman T

    更新日期:2013-12-01 00:00:00

  • Functional screening of willow alleles in Arabidopsis combined with QTL mapping in willow (Salix) identifies SxMAX4 as a coppicing response gene.

    abstract::Willows (Salix spp.) are important biomass crops due to their ability to grow rapidly with low fertilizer inputs and ease of cultivation in short-rotation coppice cycles. They are relatively undomesticated and highly diverse, but functional testing to identify useful allelic variation is time-consuming in trees and tr...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12154

    authors: Salmon J,Ward SP,Hanley SJ,Leyser O,Karp A

    更新日期:2014-05-01 00:00:00

  • Co-expression of tonoplast Cation/H(+) antiporter and H(+)-pyrophosphatase from xerophyte Zygophyllum xanthoxylum improves alfalfa plant growth under salinity, drought and field conditions.

    abstract::Salinity and drought are major environmental factors limiting the growth and productivity of alfalfa worldwide as this economically important legume forage is sensitive to these kinds of abiotic stress. In this study, transgenic alfalfa lines expressing both tonoplast NXH and H(+)-PPase genes, ZxNHX and ZxVP1-1 from t...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12451

    authors: Bao AK,Du BQ,Touil L,Kang P,Wang QL,Wang SM

    更新日期:2016-03-01 00:00:00

  • Cytokinin-mediated source/sink modifications improve drought tolerance and increase grain yield in rice under water-stress.

    abstract::Drought is the major environmental factor limiting crop productivity worldwide. We hypothesized that it is possible to enhance drought tolerance by delaying stress-induced senescence through the stress-induced synthesis of cytokinins in crop-plants. We generated transgenic rice (Oryza sativa) plants expressing an isop...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2010.00584.x

    authors: Peleg Z,Reguera M,Tumimbang E,Walia H,Blumwald E

    更新日期:2011-09-01 00:00:00