CRISPR-LbCas12a-mediated modification of citrus.

abstract：:Developing new strategies for crop plants to respond to drought is crucial for their innovative breeding. The down-regulation of nuclear cap-binding proteins in Arabidopsis renders plants drought tolerant. The CBP80 gene in the potato cultivar Desiree was silenced using artificial microRNAs. Transgenic plants displaye...

journal_title：Plant biotechnology journal

authors： Pieczynski M,Marczewski W,Hennig J,Dolata J,Bielewicz D,Piontek P,Wyrzykowska A,Krusiewicz D,Strzelczyk-Zyta D,Konopka-Postupolska D,Krzeslowska M,Jarmolowski A,Szweykowska-Kulinska Z

更新日期：2013-05-01 00:00:00

abstract：:Genome modification by homology-directed repair (HDR) is an attractive tool for the controlled genetic manipulation of plants. Here, we report the HDR-mediated gene exchange of expression cassettes in tobacco BY-2 cells using a designed zinc finger nuclease (ZFN). The target contained a 7-kb fragment flanked by two ZF...

journal_title：Plant biotechnology journal

authors： Schneider K,Schiermeyer A,Dolls A,Koch N,Herwartz D,Kirchhoff J,Fischer R,Russell SM,Cao Z,Corbin DR,Sastry-Dent L,Ainley WM,Webb SR,Schinkel H,Schillberg S

更新日期：2016-04-01 00:00:00

abstract：:To date, transgenic approaches to biofortify subsistence crops have been rather limited. This is particularly true for the starchy root crop cassava (Manihot esculenta Crantz). Cassava has one of the highest rates of CO(2) fixation and sucrose synthesis for any C3 plant, but rarely reaches its yield potentials in the ...

journal_title：Plant biotechnology journal

authors： Ihemere U,Arias-Garzon D,Lawrence S,Sayre R

更新日期：2006-07-01 00:00:00

abstract：:Whole-genome sequencing-based bulked segregant analysis (BSA) for mapping quantitative trait loci (QTL) provides an efficient alternative approach to conventional QTL analysis as it significantly reduces the scale and cost of analysis with comparable power to QTL detection using full mapping population. We tested the ...

journal_title：Plant biotechnology journal

authors： Deokar A,Sagi M,Daba K,Tar'an B

更新日期：2019-01-01 00:00:00

abstract：:For future food security, it is important that wheat, one of the most widely consumed crops in the world, can survive the threat of abiotic and biotic stresses. New genetic variation is currently being introduced into wheat through introgressions from its wild relatives. For trait discovery, it is necessary that each ...

journal_title：Plant biotechnology journal

authors： Grewal S,Hubbart-Edwards S,Yang C,Devi U,Baker L,Heath J,Ashling S,Scholefield D,Howells C,Yarde J,Isaac P,King IP,King J

更新日期：2020-03-01 00:00:00

abstract：:Traditional breeding methods are hindered in bananas due to the fact that major cultivars are sterile, parthenocarpic, triploid and thus clonally propagated. This has resulted in a narrow genetic base and limited resilience to biotic and abiotic stresses. Mutagenesis of in vitro propagated bananas is one method to int...

journal_title：Plant biotechnology journal

authors： Datta S,Jankowicz-Cieslak J,Nielen S,Ingelbrecht I,Till BJ

更新日期：2018-02-24 00:00:00

abstract：:The early methionine (Em) proteins are members of the late embryogenesis abundant (LEA) group of proteins that have been considered to be embryo specific. The ability of a 646-bp wheat Em promoter to control green fluorescent protein (gfp) expression was investigated in transgenic barley and rice. Seeds of transgenic ...

journal_title：Plant biotechnology journal

authors： Furtado A,Henry RJ

更新日期：2005-07-01 00:00:00

abstract：:Modified fatty acids (mFA) have diverse uses; for example, cyclopropane fatty acids (CPA) are feedstocks for producing coatings, lubricants, plastics and cosmetics. The expression of mFA-producing enzymes in crop and model plants generally results in lower levels of mFA accumulation than in their natural-occurring sou...

journal_title：Plant biotechnology journal

authors： Yu XH,Cahoon RE,Horn PJ,Shi H,Prakash RR,Cai Y,Hearney M,Chapman KD,Cahoon EB,Schwender J,Shanklin J

更新日期：2018-04-01 00:00:00

abstract：:Tomato is one of the most cultivated vegetables in the world and an important ingredient of the human diet. Tomato breeders and growers face a continuous challenge of combining high quantity (production volume) with high quality (appearance, taste and perception for the consumers, processing quality for the processing...

journal_title：Plant biotechnology journal

authors： Czerednik A,Busscher M,Angenent GC,de Maagd RA

更新日期：2015-02-01 00:00:00

abstract：:Pomegranate (Punica granatum L.) has an ancient cultivation history and has become an emerging profitable fruit crop due to its attractive features such as the bright red appearance and the high abundance of medicinally valuable ellagitannin-based compounds in its peel and aril. However, the limited genomic resources ...

journal_title：Plant biotechnology journal

authors： Yuan Z,Fang Y,Zhang T,Fei Z,Han F,Liu C,Liu M,Xiao W,Zhang W,Wu S,Zhang M,Ju Y,Xu H,Dai H,Liu Y,Chen Y,Wang L,Zhou J,Guan D,Yan M,Xia Y,Huang X,Liu D,Wei H,Zheng H

更新日期：2018-07-01 00:00:00

abstract：:Dengue fever is a mosquito (Aedes aegypti) -transmitted viral disease that is endemic in more than 125 countries around the world. There are four serotypes of the dengue virus (DENV 1-4) and a safe and effective dengue vaccine must provide protection against all four serotypes. To date, the first vaccine, Dengvaxia (C...

journal_title：Plant biotechnology journal

authors： van Eerde A,Gottschamel J,Bock R,Hansen KEA,Munang'andu HM,Daniell H,Liu Clarke J

更新日期：2019-07-01 00:00:00

abstract：:Spike lavender (Lavandula latifolia) essential oil is widely used in the perfume, cosmetic, flavouring and pharmaceutical industries. Thus, modifications of yield and composition of this essential oil by genetic engineering should have important scientific and commercial applications. We generated transgenic spike lav...

journal_title：Plant biotechnology journal

authors： Muñoz-Bertomeu J,Sales E,Ros R,Arrillaga I,Segura J

更新日期：2007-11-01 00:00:00

abstract：:Production of novel transgenic floricultural crops with altered petal properties requires transgenes that confer a useful trait and petal-specific promoters. Several promoters have been shown to control transgenes in petals. However, all suffer from inherent drawbacks such as low petal specificity and restricted activ...

journal_title：Plant biotechnology journal

authors： Azuma M,Morimoto R,Hirose M,Morita Y,Hoshino A,Iida S,Oshima Y,Mitsuda N,Ohme-Takagi M,Shiratake K

更新日期：2016-01-01 00:00:00

abstract：:Downy mildew (Plasmopara halstedii (Farl.) Berlese et de Toni) is a serious foliar pathogen of cultivated sunflower (Helianthus annuus L.). Genetic resistance is conditioned by several linked downy mildew resistance gene specificities in the HaRGC1 cluster of TIR-NBS-LRR resistance gene candidates (RGCs) on linkage gr...

journal_title：Plant biotechnology journal

authors： Slabaugh MB,Yu JK,Tang S,Heesacker A,Hu X,Lu G,Bidney D,Han F,Knapp SJ

更新日期：2003-05-01 00:00:00

abstract：:Ascorbate is a powerful antioxidant in plants, and its levels are an important quality criteria in commercial species. Factors influencing these levels include environmental variations, particularly light, and the genetic control of its biosynthesis, recycling and degradation. One of the genes involved in the recyclin...

journal_title：Plant biotechnology journal

authors： Gest N,Garchery C,Gautier H,Jiménez A,Stevens R

更新日期：2013-04-01 00:00:00

abstract：:The nitrate (NO3-) transporter has been selected as an important gene maker in the process of environmental adoption in rice cultivars. In this work, we transferred another native OsNAR2.1 promoter with driving OsNAR2.1 gene into rice plants. The transgenic lines with exogenous pOsNAR2.1:OsNAR2.1 constructs showed enh...

journal_title：Plant biotechnology journal

authors： Chen J,Fan X,Qian K,Zhang Y,Song M,Liu Y,Xu G,Fan X

更新日期：2017-10-01 00:00:00

abstract：:Although much explored, oral tolerance for treatment of autoimmune diseases still awaits the establishment of novel and effective vectors. We investigated whether the tolerogenic CTA1(R7K)-COL-DD fusion protein can be expressed in edible plants, to induce oral tolerance and protect against arthritis. The fusion protei...

journal_title：Plant biotechnology journal

authors： Hansson C,Schön K,Kalbina I,Strid Å,Andersson S,Bokarewa MI,Lycke NY

更新日期：2016-04-01 00:00:00

abstract：:Recent report reviews and funding initiatives in the field of plant genomic research are considered in the context of their translation into practical and economic value via plant breeding. It is concluded that there is a deficit in investment and that a change in working styles towards knowledge sharing and connectiv...

journal_title：Plant biotechnology journal

authors： Hughes S

更新日期：2006-01-01 00:00:00

abstract：:Suppression of the microsomal ω6 oleate desaturase during the seed development of soybean (Glycine max) with the 420-bp soybean FAD2-1A intron as RNAi trigger shifts the conventional fatty acid composition of soybean oil from 20% oleic and 60% polyunsaturates to one containing greater than 80% oleic acid and less than...

journal_title：Plant biotechnology journal

authors： Wagner N,Mroczka A,Roberts PD,Schreckengost W,Voelker T

更新日期：2011-09-01 00:00:00

abstract：:It is widely recognized that biofuel production from lignocellulosic materials is limited by inadequate technology to efficiently and economically release fermentable sugars from the complex multi-polymeric raw materials. Therefore, endoglucanases, exoglucanase, pectate lyases, cutinase, swollenin, xylanase, acetyl xy...

journal_title：Plant biotechnology journal

authors： Verma D,Kanagaraj A,Jin S,Singh ND,Kolattukudy PE,Daniell H

更新日期：2010-04-01 00:00:00

abstract：:Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer c...

journal_title：Plant biotechnology journal

authors： Ramírez-Estrada K,Altabella T,Onrubia M,Moyano E,Notredame C,Osuna L,Vanden Bossche R,Goossens A,Cusido RM,Palazon J

更新日期：2016-01-01 00:00:00

abstract：:Glyphosate is a popular, systemic, broad-spectrum herbicide used in modern agriculture. Being a structural analog of phosphoenolpyruvate (PEP), it inhibits 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) which is responsible for the biosynthesis of aromatic amino acids and various aromatic secondary metabolites. T...

journal_title：Plant biotechnology journal

authors： Achary VMM,Sheri V,Manna M,Panditi V,Borphukan B,Ram B,Agarwal A,Fartyal D,Teotia D,Masakapalli SK,Agrawal PK,Reddy MK

更新日期：2020-12-01 00:00:00

abstract：:A high throughput proton nuclear magnetic resonance spectroscopy method for the metabolite fingerprinting of plants was applied to genetically modified peas (Pisum sativum) to determine whether biochemical changes, so called 'unintended effects', beyond those intended by incorporation of a transgene, were detectable. ...

journal_title：Plant biotechnology journal

authors： Charlton A,Allnutt T,Holmes S,Chisholm J,Bean S,Ellis N,Mullineaux P,Oehlschlager S

更新日期：2004-01-01 00:00:00

abstract：:The rice α-amylase 3D promoter system, which is activated under sucrose-starved conditions, has emerged as a useful system for producing recombinant proteins. However, using rice as the production system for therapeutic proteins requires modifications of the N-glycosylation pattern because of the potential immunogenic...

journal_title：Plant biotechnology journal

authors： Shin YJ,Chong YJ,Yang MS,Kwon TH

更新日期：2011-12-01 00:00:00

abstract：:Genetic improvement of fibre quality is one of the main breeding goals for the upland cotton, Gossypium hirsutum, but there are difficulties with precise selection of traits. Therefore, it is important to improve the understanding of the genetic basis of phenotypic variation. In this study, we conducted phenotyping an...

journal_title：Plant biotechnology journal

authors： Sun Z,Wang X,Liu Z,Gu Q,Zhang Y,Li Z,Ke H,Yang J,Wu J,Wu L,Zhang G,Zhang C,Ma Z

更新日期：2017-08-01 00:00:00

abstract：:In order to create a novel mechanism for herbicide resistance in plants, we expressed a single-chain antibody fragment (scFv) in tobacco with specific affinity to the auxinic herbicide picloram. Transgenic tobacco plants and seedlings expressing this scFv against picloram were protected from its effect in a dose-depen...

journal_title：Plant biotechnology journal

authors： Almquist KC,Niu Y,McLean MD,Mena FL,Yau KY,Brown K,Brandle JE,Hall JC

更新日期：2004-05-01 00:00:00

abstract：:Orobanche spp. (broomrape) are parasitic plants which subsist on the roots of a wide range of hosts, including tomato, causing severe losses in yield quality and quantity. Large amounts of mannitol accumulate in this parasitic weed during development. Mannose 6-phosphate reductase (M6PR) is a key enzyme in mannitol bi...

journal_title：Plant biotechnology journal

authors： Aly R,Cholakh H,Joel DM,Leibman D,Steinitz B,Zelcer A,Naglis A,Yarden O,Gal-On A

更新日期：2009-08-01 00:00:00

abstract：:Willows (Salix spp.) are important biomass crops due to their ability to grow rapidly with low fertilizer inputs and ease of cultivation in short-rotation coppice cycles. They are relatively undomesticated and highly diverse, but functional testing to identify useful allelic variation is time-consuming in trees and tr...

journal_title：Plant biotechnology journal

authors： Salmon J,Ward SP,Hanley SJ,Leyser O,Karp A

更新日期：2014-05-01 00:00:00

abstract：:Steviol glycosides (SGs) are extracted from Stevia leaves for use as a natural sweetener. Among SGs, stevioside is most abundant in leaf extracts followed by rebaudioside A (Reb A). However, Reb A is of particular interest because of its sweeter and more pleasant taste compared to stevioside. Therefore, the developmen...

journal_title：Plant biotechnology journal

authors： Kim MJ,Zheng J,Liao MH,Jang IC

更新日期：2019-06-01 00:00:00

abstract：:The feasibility of using the FLP/FRT site-specific recombination system in rice for genome engineering was evaluated. Transgenic rice plants expressing the FLP recombinase were crossed with plants harbouring the kanamycin resistance gene (neomycin phosphotransferase II, nptII) flanked by FRT sites, which also served t...

journal_title：Plant biotechnology journal

authors： Hu Q,Kononowicz-Hodges H,Nelson-Vasilchik K,Viola D,Zeng P,Liu H,Kausch AP,Chandlee JM,Hodges TK,Luo H

更新日期：2008-02-01 00:00:00