Abstract:
:The subspecies fastigiata of cultivated groundnut lost fresh seed dormancy (FSD) during domestication and human-made selection. Groundnut varieties lacking FSD experience precocious seed germination during harvest imposing severe losses. Development of easy-to-use genetic markers enables early-generation selection in different molecular breeding approaches. In this context, one recombinant inbred lines (RIL) population (ICGV 00350 × ICGV 97045) segregating for FSD was used for deploying QTL-seq approach for identification of key genomic regions and candidate genes. Whole-genome sequencing (WGS) data (87.93 Gbp) were generated and analysed for the dormant parent (ICGV 97045) and two DNA pools (dormant and nondormant). After analysis of resequenced data from the pooled samples with dormant parent (reference genome), we calculated delta-SNP index and identified a total of 10,759 genomewide high-confidence SNPs. Two candidate genomic regions spanning 2.4 Mb and 0.74 Mb on the B05 and A09 pseudomolecules, respectively, were identified controlling FSD. Two candidate genes-RING-H2 finger protein and zeaxanthin epoxidase-were identified in these two regions, which significantly express during seed development and control abscisic acid (ABA) accumulation. QTL-seq study presented here laid out development of a marker, GMFSD1, which was validated on a diverse panel and could be used in molecular breeding to improve dormancy in groundnut.
journal_name
Plant Biotechnol Jjournal_title
Plant biotechnology journalauthors
Kumar R,Janila P,Vishwakarma MK,Khan AW,Manohar SS,Gangurde SS,Variath MT,Shasidhar Y,Pandey MK,Varshney RKdoi
10.1111/pbi.13266subject
Has Abstractpub_date
2020-04-01 00:00:00pages
992-1003issue
4eissn
1467-7644issn
1467-7652journal_volume
18pub_type
杂志文章abstract::Expression of industrial enzymes in transgenic plants offers an alternative system to fungal fermentation for large-scale production. Very high levels of expression are required to make the enzymes cost-effective. We tested several parameters to determine the best method for achieving high levels of expression for a f...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1046/j.1467-7652.2003.00014.x
更新日期:2003-03-01 00:00:00
abstract::Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer c...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12359
更新日期:2016-01-01 00:00:00
abstract::We constructed a novel autonomously replicating gene expression shuttle vector, with the aim of developing a system for transiently expressing proteins at levels useful for commercial production of vaccines and other proteins in plants. The vector, pRIC, is based on the mild strain of the geminivirus Bean yellow dwarf...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2009.00462.x
更新日期:2010-01-01 00:00:00
abstract::Tef, Eragrostis tef (Zucc.) Trotter, is the most important cereal in Ethiopia. Tef is cultivated by more than five million small-scale farmers annually and constitutes the staple food for more than half of the population of 80 million. The crop is preferred by both farmers and consumers due to its beneficial traits as...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/pbi.12199
更新日期:2014-06-01 00:00:00
abstract::The rice α-amylase 3D promoter system, which is activated under sucrose-starved conditions, has emerged as a useful system for producing recombinant proteins. However, using rice as the production system for therapeutic proteins requires modifications of the N-glycosylation pattern because of the potential immunogenic...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2011.00636.x
更新日期:2011-12-01 00:00:00
abstract::Dioxin residues widely contaminate soil and agricultural products at low concentrations and may accumulate in organisms at the top of food chains owing to their physicochemical properties. In this study, we have developed novel, dioxin-inducible, reporter gene expression systems regulated by recombinant aryl hydrocarb...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2008.00378.x
更新日期:2009-02-01 00:00:00
abstract::Woody biomass has gained popularity as an environmentally friendly, renewable and sustainable resource for liquid fuel production. Here, we demonstrate biotechnological improvement of the quantity and quality of woody biomass by employing developing xylem (DX)-preferential production of gibberellin (GA), a phytohormon...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12484
更新日期:2016-04-01 00:00:00
abstract::Which factors limit metabolite accumulation in plant cells? Are theories on flux control effective at explaining the results? Many biotechnologists cling to the idea that every pathway has a rate limiting enzyme and target such enzymes first in order to modulate fluxes. This often translates into large effects on meta...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/pbi.12035
更新日期:2013-02-01 00:00:00
abstract::Increased use of nitrogen fertilizers has deleterious impact on the environment. Increase in yield potential at low nitrogen supply is regarded as a cereal breeding goal for future agricultural sustainability. Although natural variations of nitrogen transporters have been investigated, key genes associated with assimi...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13450
更新日期:2021-01-01 00:00:00
abstract::Modified fatty acids (mFA) have diverse uses; for example, cyclopropane fatty acids (CPA) are feedstocks for producing coatings, lubricants, plastics and cosmetics. The expression of mFA-producing enzymes in crop and model plants generally results in lower levels of mFA accumulation than in their natural-occurring sou...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12839
更新日期:2018-04-01 00:00:00
abstract::Reverse transcription-polymerase chain reaction (RT-PCR) approaches have been used in a large proportion of transcriptome analyses published to date. The accuracy of the results obtained by this method strongly depends on accurate transcript normalization using stably expressed genes, known as references. Statistical ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2008.00346.x
更新日期:2008-08-01 00:00:00
abstract::The feasibility of using the FLP/FRT site-specific recombination system in rice for genome engineering was evaluated. Transgenic rice plants expressing the FLP recombinase were crossed with plants harbouring the kanamycin resistance gene (neomycin phosphotransferase II, nptII) flanked by FRT sites, which also served t...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2007.00310.x
更新日期:2008-02-01 00:00:00
abstract::Grain size, number and starch content are important determinants of grain yield and quality. One of the most important biological processes that determine these components is the carbon partitioning during the early grain filling, which requires the function of cell wall invertase. Here, we showed the constitutive exp...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12102
更新日期:2013-12-01 00:00:00
abstract::In the last 20 years, stem rust caused by the fungus Puccinia graminis f. sp. tritici (Pgt), has re-emerged as a major threat to wheat and barley production in Africa and Europe. In contrast to wheat with 60 designated stem rust (Sr) resistance genes, barley's genetic variation for stem rust resistance is very narrow ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13460
更新日期:2020-08-03 00:00:00
abstract::Globally, wheat is the most widely grown crop and one of the three most important crops for human and livestock feed. However, the complex nature of the wheat genome has, until recently, resulted in a lack of single nucleotide polymorphism (SNP)-based molecular markers of practical use to wheat breeders. Recently, lar...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12009
更新日期:2013-04-01 00:00:00
abstract::The production of biodegradable polymers that can be used to substitute petrochemical compounds in commercial products in transgenic plants is an important challenge for plant biotechnology. Nevertheless, it is often accompanied by reduced plant fitness. To decrease the phenotypic abnormalities of the sprout and to in...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2009.00451.x
更新日期:2009-12-01 00:00:00
abstract::Eastern cottonwood (Populus deltoides Bartr. ex Marsh.) trees were engineered to express merA (mercuric ion reductase) and merB (organomercury lyase) transgenes in order to be used for the phytoremediation of mercury-contaminated soils. Earlier studies with Arabidopsis thaliana and Nicotiana tabacum showed that this g...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2006.00236.x
更新日期:2007-03-01 00:00:00
abstract::In this study, the effects of the accumulation of cysteine protease inhibitors on the food preferences of adult female western flower thrips, Frankliniella occidentalis (Pergande), were investigated. Representative members of the cystatin and thyropin gene families (stefin A, cystatin C, kininogen domain 3 and equista...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2004.00088.x
更新日期:2004-09-01 00:00:00
abstract::The cooking quality of rice is associated with the starch gelatinization temperature (GT). Rice genotypes with low GT have probably been selected for their cooking quality by humans during domestication. We now report polymorphisms in starch synthase IIa (SSIIa) that explain the variation in rice starch GT. Sequence a...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2005.00162.x
更新日期:2006-01-01 00:00:00
abstract::Soya bean (Glycine max) and grass pea (Lathyrus sativus) seeds are important sources of dietary proteins; however, they also contain antinutritional metabolite oxalic acid (OA). Excess dietary intake of OA leads to nephrolithiasis due to the formation of calcium oxalate crystals in kidneys. Besides, OA is also a known...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12503
更新日期:2016-06-01 00:00:00
abstract::Development of resistance to the insecticidal toxins from Bacillus thuringiensis (Bt) in insects is the major threat to the continued success of transgenic Bt crops in agriculture. The fitness of Bt-resistant insects on Bt and non-Bt plants is a key parameter that determines the development of Bt resistance in insect ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12718
更新日期:2017-10-01 00:00:00
abstract::Plants offer fast, flexible and easily scalable alternative platforms for the production of pharmaceutical proteins, but differences between plant and mammalian N-linked glycans, including the presence of β-1,2-xylose and core α-1,3-fucose residues in plants, can affect the activity, potency and immunogenicity of plan...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12981
更新日期:2019-02-01 00:00:00
abstract::Pollen germination and pollen tube growth are important physiological processes of sexual reproduction of plants and also are involved in signal transduction. Our previous study reveals that ZmSTK1 and ZmSTK2 are two receptor-like cytoplasmic kinases (RLCK) homologs in Zea mays as members of receptor-like protein kina...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12880
更新日期:2018-08-01 00:00:00
abstract::Advances in the genetic transformation of cereals have improved the prospects of using biotechnology for plant improvement, and a toolbox of promoters with defined specificities would be a valuable resource in controlling the expression of transgenes in desired tissues for both plant improvement and molecular farming....
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2008.00394.x
更新日期:2009-04-01 00:00:00
abstract::Steviol glycosides (SGs) are extracted from Stevia leaves for use as a natural sweetener. Among SGs, stevioside is most abundant in leaf extracts followed by rebaudioside A (Reb A). However, Reb A is of particular interest because of its sweeter and more pleasant taste compared to stevioside. Therefore, the developmen...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13035
更新日期:2019-06-01 00:00:00
abstract::The root system is fundamental for plant development, is crucial for overall plant growth and is recently being recognized as the key for future crop productivity improvement. A major determinant of root system architecture is the initiation of lateral roots. While knowledge of the genetic and molecular mechanisms reg...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12104
更新日期:2013-12-01 00:00:00
abstract::Genetic improvement of fibre quality is one of the main breeding goals for the upland cotton, Gossypium hirsutum, but there are difficulties with precise selection of traits. Therefore, it is important to improve the understanding of the genetic basis of phenotypic variation. In this study, we conducted phenotyping an...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12693
更新日期:2017-08-01 00:00:00
abstract::The early methionine (Em) proteins are members of the late embryogenesis abundant (LEA) group of proteins that have been considered to be embryo specific. The ability of a 646-bp wheat Em promoter to control green fluorescent protein (gfp) expression was investigated in transgenic barley and rice. Seeds of transgenic ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2005.00135.x
更新日期:2005-07-01 00:00:00
abstract::Cadmium (Cd) is a widespread soil contaminant threatening human health. As an ideal energy plant, sweet sorghum (Sorghum bicolor (L.) Moench) has great potential in phytoremediation of Cd-polluted soils, although the molecular mechanisms are largely unknown. In this study, key factors responsible for differential Cd a...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12795
更新日期:2018-02-01 00:00:00
abstract::The plant pathogen, Pseudomonas syringae (Ps), together with related Ps species, infects and attacks a wide range of agronomically important crops, including tomato, kiwifruit, pepper, olive and soybean, causing economic losses. Currently, chemicals and introduced resistance genes are used to protect plants against th...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13294
更新日期:2020-05-01 00:00:00