Up-regulation of an N-terminal truncated 3-hydroxy-3-methylglutaryl CoA reductase enhances production of essential oils and sterols in transgenic Lavandula latifolia.

Abstract:

:Spike lavender (Lavandula latifolia) essential oil is widely used in the perfume, cosmetic, flavouring and pharmaceutical industries. Thus, modifications of yield and composition of this essential oil by genetic engineering should have important scientific and commercial applications. We generated transgenic spike lavender plants expressing the Arabidopsis thaliana HMG1 cDNA, encoding the catalytic domain of 3-hydroxy-3-methylglutaryl CoA reductase (HMGR1S), a key enzyme of the mevalonic acid (MVA) pathway. Transgenic T0 plants accumulated significantly more essential oil constituents as compared to controls (up to 2.1- and 1.8-fold in leaves and flowers, respectively). Enhanced expression of HMGR1S also increased the amount of the end-product sterols, beta-sitosterol and stigmasterol (average differences of 1.8- and 1.9-fold, respectively), but did not affect the accumulation of carotenoids or chlorophylls. We also analysed T1 plants derived from self-pollinated seeds of T0 lines that flowered after growing for 2 years in the greenhouse. The increased levels of essential oil and sterols observed in the transgenic T0 plants were maintained in the progeny that inherited the HMG1 transgene. Our results demonstrate that genetic manipulation of the MVA pathway increases essential oil yield in spike lavender, suggesting a contribution for this cytosolic pathway to monoterpene and sesquiterpene biosynthesis in leaves and flowers of the species.

journal_name

Plant Biotechnol J

authors

Muñoz-Bertomeu J,Sales E,Ros R,Arrillaga I,Segura J

doi

10.1111/j.1467-7652.2007.00286.x

subject

Has Abstract

pub_date

2007-11-01 00:00:00

pages

746-58

issue

6

eissn

1467-7644

issn

1467-7652

pii

PBI286

journal_volume

5

pub_type

杂志文章
  • Divergent improvement of two cultivated allotetraploid cotton species.

    abstract::Interspecific genomic variation can provide a genetic basis for local adaptation and domestication. A series of studies have presented its role of interspecific haplotypes and introgressions in adaptive traits, but few studies have addressed their role in improving agronomic character. Two allotetraploid Gossypium spe...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13547

    authors: Fang L,Zhao T,Hu Y,Si Z,Zhu X,Han Z,Liu G,Wang S,Ju L,Guo M,Mei H,Wang L,Qi B,Wang H,Guan X,Zhang T

    更新日期:2021-01-14 00:00:00

  • A maize phytochrome-interacting factors protein ZmPIF1 enhances drought tolerance by inducing stomatal closure and improves grain yield in Oryza sativa.

    abstract::Phytochrome-interacting factors (PIFs) play major roles in regulating plant growth and development, but their roles in drought stress remain elusive. Here, we cloned and characterized a maize (Zea mays) PIF transcription factor, ZmPIF1. The expression level of ZmPIF1 was significantly induced by independent drought an...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12878

    authors: Gao Y,Wu M,Zhang M,Jiang W,Ren X,Liang E,Zhang D,Zhang C,Xiao N,Li Y,Dai Y,Chen J

    更新日期:2018-07-01 00:00:00

  • Sequencing of Australian wild rice genomes reveals ancestral relationships with domesticated rice.

    abstract::The related A genome species of the Oryza genus are the effective gene pool for rice. Here, we report draft genomes for two Australian wild A genome taxa: O. rufipogon-like population, referred to as Taxon A, and O. meridionalis-like population, referred to as Taxon B. These two taxa were sequenced and assembled by in...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12674

    authors: Brozynska M,Copetti D,Furtado A,Wing RA,Crayn D,Fox G,Ishikawa R,Henry RJ

    更新日期:2017-06-01 00:00:00

  • An oligosaccharyltransferase from Leishmania major increases the N-glycan occupancy on recombinant glycoproteins produced in Nicotiana benthamiana.

    abstract::N-glycosylation is critical for recombinant glycoprotein production as it influences the heterogeneity of products and affects their biological function. In most eukaryotes, the oligosaccharyltransferase is the central-protein complex facilitating the N-glycosylation of proteins in the lumen of the endoplasmic reticul...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12906

    authors: Castilho A,Beihammer G,Pfeiffer C,Göritzer K,Montero-Morales L,Vavra U,Maresch D,Grünwald-Gruber C,Altmann F,Steinkellner H,Strasser R

    更新日期:2018-10-01 00:00:00

  • Engineering linear, branched-chain triterpene metabolism in monocots.

    abstract::Triterpenes are thirty-carbon compounds derived from the universal five-carbon prenyl precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Normally, triterpenes are synthesized via the mevalonate (MVA) pathway operating in the cytoplasm of eukaryotes where DMAPP is condensed with two IPPs to...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12983

    authors: Kempinski C,Jiang Z,Zinck G,Sato SJ,Ge Z,Clemente TE,Chappell J

    更新日期:2019-02-01 00:00:00

  • A global view of transcriptome dynamics during flower development in chickpea by deep sequencing.

    abstract::Measurement of gene expression can provide important clues about gene function and molecular basis of developmental processes. Here, we have analysed the chickpea transcriptome in vegetative and flower tissues by exploiting the potential of high-throughput sequencing to measure gene expression. We mapped more than 295...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12059

    authors: Singh VK,Garg R,Jain M

    更新日期:2013-08-01 00:00:00

  • Immunomodulation confers herbicide resistance in plants.

    abstract::In order to create a novel mechanism for herbicide resistance in plants, we expressed a single-chain antibody fragment (scFv) in tobacco with specific affinity to the auxinic herbicide picloram. Transgenic tobacco plants and seedlings expressing this scFv against picloram were protected from its effect in a dose-depen...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2004.00060.x

    authors: Almquist KC,Niu Y,McLean MD,Mena FL,Yau KY,Brown K,Brandle JE,Hall JC

    更新日期:2004-05-01 00:00:00

  • Agrobacterium tumefaciens-mediated sorghum transformation using a mannose selection system.

    abstract::A dual-marker plasmid containing the selectable marker gene, manA, and the reporter gene, sgfp, was used to transform immature sorghum embryos by employing an Agrobacterium-mediated system. Both genes were under the control of the ubi1 promoter in a binary vector pPZP201. The Escherichia coli phosphomannose isomerase ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2005.00150.x

    authors: Gao Z,Xie X,Ling Y,Muthukrishnan S,Liang GH

    更新日期:2005-11-01 00:00:00

  • The genetic architecture of the dynamic changes in grain moisture in maize.

    abstract::Low grain moisture at harvest is crucial for safe production, transport, and storage, but the genetic architecture of this trait in maize (Zea mays) remains elusive. Here, we measured the dynamic changes in grain moisture content in an association mapping panel of 513 diverse maize inbred lines at five successive stag...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13541

    authors: Li W,Yu Y,Wang L,Luo Y,Peng Y,Xu Y,Liu X,Wu S,Jian L,Xu J,Xiao Y,Yan J

    更新日期:2021-01-01 00:00:00

  • Pinellia ternata agglutinin expression in chloroplasts confers broad spectrum resistance against aphid, whitefly, Lepidopteran insects, bacterial and viral pathogens.

    abstract::Broad spectrum protection against different insects and pathogens requires multigene engineering. However, such broad spectrum protection against biotic stress is provided by a single protein in some medicinal plants. Therefore, tobacco chloroplasts were transformed with the agglutinin gene from Pinellia ternata (pta)...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2011.00663.x

    authors: Jin S,Zhang X,Daniell H

    更新日期:2012-04-01 00:00:00

  • The pomegranate (Punica granatum L.) genome provides insights into fruit quality and ovule developmental biology.

    abstract::Pomegranate (Punica granatum L.) has an ancient cultivation history and has become an emerging profitable fruit crop due to its attractive features such as the bright red appearance and the high abundance of medicinally valuable ellagitannin-based compounds in its peel and aril. However, the limited genomic resources ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12875

    authors: Yuan Z,Fang Y,Zhang T,Fei Z,Han F,Liu C,Liu M,Xiao W,Zhang W,Wu S,Zhang M,Ju Y,Xu H,Dai H,Liu Y,Chen Y,Wang L,Zhou J,Guan D,Yan M,Xia Y,Huang X,Liu D,Wei H,Zheng H

    更新日期:2018-07-01 00:00:00

  • T-DNA locus structure in a large population of soybean plants transformed using the Agrobacterium-mediated cotyledonary-node method.

    abstract::Designing transformation experiments for either functional genomics or crop improvement requires knowledge of the transgene locus structure, number, transmission and expression resulting from a specific transformation method. We recently reported an improvement to the soybean [Glycine max (L.) Merrill] cotyledonary-no...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2004.00070.x

    authors: Olhoft PM,Flagel LE,Somers DA

    更新日期:2004-07-01 00:00:00

  • Enhancing the expression of starch synthase class IV results in increased levels of both transitory and long-term storage starch.

    abstract::Starch is an important renewable raw material with an increasing number of applications. Several attempts have been made to obtain plants that produce modified versions of starch or higher starch yield. Most of the approaches designed to increase the levels of starch have focused on the increment of the amount of ADP-...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2011.00626.x

    authors: Gámez-Arjona FM,Li J,Raynaud S,Baroja-Fernández E,Muñoz FJ,Ovecka M,Ragel P,Bahaji A,Pozueta-Romero J,Mérida Á

    更新日期:2011-12-01 00:00:00

  • Opinion piece: genomics and crop plant science in Europe.

    abstract::Recent report reviews and funding initiatives in the field of plant genomic research are considered in the context of their translation into practical and economic value via plant breeding. It is concluded that there is a deficit in investment and that a change in working styles towards knowledge sharing and connectiv...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2005.00164.x

    authors: Hughes S

    更新日期:2006-01-01 00:00:00

  • RNAi trigger fragment truncation attenuates soybean FAD2-1 transcript suppression and yields intermediate oil phenotypes.

    abstract::Suppression of the microsomal ω6 oleate desaturase during the seed development of soybean (Glycine max) with the 420-bp soybean FAD2-1A intron as RNAi trigger shifts the conventional fatty acid composition of soybean oil from 20% oleic and 60% polyunsaturates to one containing greater than 80% oleic acid and less than...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2010.00573.x

    authors: Wagner N,Mroczka A,Roberts PD,Schreckengost W,Voelker T

    更新日期:2011-09-01 00:00:00

  • Down-regulated expression of plant-specific glycoepitopes in alfalfa.

    abstract:SUMMARY:Compared with other plant expression systems used for pharmaceutical protein production, alfalfa offers the advantage of very homogeneous N-glycosylation. Therefore, this plant was selected for further attempts at glycoengineering. Two main approaches were developed in order to humanize N-glycosylation in alfal...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2008.00353.x

    authors: Sourrouille C,Marquet-Blouin E,D'Aoust MA,Kiefer-Meyer MC,Seveno M,Pagny-Salehabadi S,Bardor M,Durambur G,Lerouge P,Vezina L,Gomord V

    更新日期:2008-09-01 00:00:00

  • The cell size distribution of tomato fruit can be changed by overexpression of CDKA1.

    abstract::Tomato is one of the most cultivated vegetables in the world and an important ingredient of the human diet. Tomato breeders and growers face a continuous challenge of combining high quantity (production volume) with high quality (appearance, taste and perception for the consumers, processing quality for the processing...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12268

    authors: Czerednik A,Busscher M,Angenent GC,de Maagd RA

    更新日期:2015-02-01 00:00:00

  • A petal-specific InMYB1 promoter from Japanese morning glory: a useful tool for molecular breeding of floricultural crops.

    abstract::Production of novel transgenic floricultural crops with altered petal properties requires transgenes that confer a useful trait and petal-specific promoters. Several promoters have been shown to control transgenes in petals. However, all suffer from inherent drawbacks such as low petal specificity and restricted activ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12389

    authors: Azuma M,Morimoto R,Hirose M,Morita Y,Hoshino A,Iida S,Oshima Y,Mitsuda N,Ohme-Takagi M,Shiratake K

    更新日期:2016-01-01 00:00:00

  • Genetic and metabolic effects of ripening mutations and vine detachment on tomato fruit quality.

    abstract::Tomato (Solanum lycopersicum) fruit ripening is regulated co-operatively by the action of ethylene and a hierarchy of transcription factors, including RIPENING INHIBITOR (RIN) and NON-RIPENING (NOR). Mutations in these two genes have been adopted commercially to delay ripening, and accompanying textural deterioration,...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13176

    authors: Osorio S,Carneiro RT,Lytovchenko A,McQuinn R,Sørensen I,Vallarino JG,Giovannoni JJ,Fernie AR,Rose JKC

    更新日期:2020-01-01 00:00:00

  • Commercialization of new biotechnology: a systematic review of 16 commercial case studies in a novel manufacturing sector.

    abstract::The 1980s and 1990s saw a major expansion of biotechnology into new areas of science including genomics and recombinant technologies. This was coupled to the widespread emergence of academics into the commercial sector as they were encouraged to spin out companies or commercialize their intellectual property. There we...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/pbi.12426

    authors: Paul MJ,Thangaraj H,Ma JK

    更新日期:2015-10-01 00:00:00

  • Revealing frequent alternative polyadenylation and widespread low-level transcription read-through of novel plant transcription terminators.

    abstract::Plant genetic engineering can create transgenic crops with improved characteristics by introducing trait genes through transformation. Appropriate regulatory elements such as promoters and terminators have to be present in certain configurations for the transgenes to be properly expressed. Five terminators native to s...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2010.00504.x

    authors: Xing A,Moon BP,Mills KM,Falco SC,Li Z

    更新日期:2010-09-01 00:00:00

  • Characterization of disease resistance genes in the Brassica napus pangenome reveals significant structural variation.

    abstract::Methods based on single nucleotide polymorphism (SNP), copy number variation (CNV) and presence/absence variation (PAV) discovery provide a valuable resource to study gene structure and evolution. However, as a result of these structural variations, a single reference genome is unable to cover the entire gene content ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13262

    authors: Dolatabadian A,Bayer PE,Tirnaz S,Hurgobin B,Edwards D,Batley J

    更新日期:2020-04-01 00:00:00

  • Genetic modification of cassava for enhanced starch production.

    abstract::To date, transgenic approaches to biofortify subsistence crops have been rather limited. This is particularly true for the starchy root crop cassava (Manihot esculenta Crantz). Cassava has one of the highest rates of CO(2) fixation and sucrose synthesis for any C3 plant, but rarely reaches its yield potentials in the ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2006.00195.x

    authors: Ihemere U,Arias-Garzon D,Lawrence S,Sayre R

    更新日期:2006-07-01 00:00:00

  • Resistance and biomass in Arabidopsis: a new model for salicylic acid perception.

    abstract::Salicylic acid (SA) is an essential hormone for plant defence and development. SA perception is usually measured by counting the number of pathogens that grow in planta upon an exogenous application of the hormone. A biological SA perception model based on plant fresh weight reduction caused by disease resistance in A...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2009.00468.x

    authors: Canet JV,Dobón A,Ibáñez F,Perales L,Tornero P

    更新日期:2010-02-01 00:00:00

  • Constitutive expression of cell wall invertase genes increases grain yield and starch content in maize.

    abstract::Grain size, number and starch content are important determinants of grain yield and quality. One of the most important biological processes that determine these components is the carbon partitioning during the early grain filling, which requires the function of cell wall invertase. Here, we showed the constitutive exp...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12102

    authors: Li B,Liu H,Zhang Y,Kang T,Zhang L,Tong J,Xiao L,Zhang H

    更新日期:2013-12-01 00:00:00

  • Expression of pigeonpea hybrid-proline-rich protein encoding gene (CcHyPRP) in yeast and Arabidopsis affords multiple abiotic stress tolerance.

    abstract::A hybrid-proline-rich protein encoding gene (CcHyPRP) has been isolated and characterized, for the first time, from the subtracted cDNA library of pigeonpea (Cajanus cajan L.) plants subjected to drought stress. Functionality of CcHyPRP has been validated for abiotic stress tolerance using the heterologous yeast and A...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2009.00467.x

    authors: Priyanka B,Sekhar K,Reddy VD,Rao KV

    更新日期:2010-01-01 00:00:00

  • GbSOBIR1 confers Verticillium wilt resistance by phosphorylating the transcriptional factor GbbHLH171 in Gossypium barbadense.

    abstract::Receptor-like kinases (RLKs) are important components of plant innate immunity. Although recent studies have revealed that the RLK suppressor of BIR1-1 (SOBIR1) can interact with multiple receptor-like proteins and is required for resistance against fungal pathogens, how the signal is transduced and triggers immune re...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12954

    authors: Zhou Y,Sun L,Wassan GM,He X,Shaban M,Zhang L,Zhu L,Zhang X

    更新日期:2019-01-01 00:00:00

  • High level protein expression in plants through the use of a novel autonomously replicating geminivirus shuttle vector.

    abstract::We constructed a novel autonomously replicating gene expression shuttle vector, with the aim of developing a system for transiently expressing proteins at levels useful for commercial production of vaccines and other proteins in plants. The vector, pRIC, is based on the mild strain of the geminivirus Bean yellow dwarf...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2009.00462.x

    authors: Regnard GL,Halley-Stott RP,Tanzer FL,Hitzeroth II,Rybicki EP

    更新日期:2010-01-01 00:00:00

  • Overexpression of S-adenosyl-L-methionine synthetase increased tomato tolerance to alkali stress through polyamine metabolism.

    abstract::S-adenosyl-L-methionine (SAM) synthetase is the key enzyme involved in the biosynthesis of SAM, which serves as a common precursor for polyamines (PAs) and ethylene. A SAM synthetase cDNA (SlSAMS1) was introduced into the tomato genome using the Agrobacterium tumefaciens transformation method. Transgenic plants overex...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12173

    authors: Gong B,Li X,VandenLangenberg KM,Wen D,Sun S,Wei M,Li Y,Yang F,Shi Q,Wang X

    更新日期:2014-08-01 00:00:00

  • Overexpression of SrUGT76G1 in Stevia alters major steviol glycosides composition towards improved quality.

    abstract::Steviol glycosides (SGs) are extracted from Stevia leaves for use as a natural sweetener. Among SGs, stevioside is most abundant in leaf extracts followed by rebaudioside A (Reb A). However, Reb A is of particular interest because of its sweeter and more pleasant taste compared to stevioside. Therefore, the developmen...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13035

    authors: Kim MJ,Zheng J,Liao MH,Jang IC

    更新日期:2019-06-01 00:00:00