Abstract:
:A precursor of miR156 (MsmiR156d) was cloned and overexpressed in alfalfa (Medicago sativa L.) as a means to enhance alfalfa biomass yield. Of the five predicted SPL genes encoded by the alfalfa genome, three (SPL6, SPL12 and SPL13) contain miR156 cleavage sites and their expression was down-regulated in transgenic alfalfa plants overexpressing miR156. These transgenic plants had reduced internode length and stem thickness, enhanced shoot branching, increased trichome density, a delay in flowering time and elevated biomass production. Minor effects on sugar, starch, lignin and cellulose contents were also observed. Moreover, transgenic alfalfa plants had increased root length, while nodulation was maintained. The multitude of traits affected by miR156 may be due to the network of genes regulated by the three target SPLs. Our results show that the miR156/SPL system has strong potential as a tool to substantially improve quality and yield traits in alfalfa.
journal_name
Plant Biotechnol Jjournal_title
Plant biotechnology journalauthors
Aung B,Gruber MY,Amyot L,Omari K,Bertrand A,Hannoufa Adoi
10.1111/pbi.12308subject
Has Abstractpub_date
2015-08-01 00:00:00pages
779-90issue
6eissn
1467-7644issn
1467-7652journal_volume
13pub_type
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