Abstract:
:Downy mildew (Plasmopara halstedii (Farl.) Berlese et de Toni) is a serious foliar pathogen of cultivated sunflower (Helianthus annuus L.). Genetic resistance is conditioned by several linked downy mildew resistance gene specificities in the HaRGC1 cluster of TIR-NBS-LRR resistance gene candidates (RGCs) on linkage group 8. The complexity and diversity of the HaRGC1 cluster was assessed by multilocus intron fragment length polymorphism (IFLP) genotyping using a single pair of primers flanking a hypervariable intron located between the TIR and NBS domains. Two to 23 bands were amplified per germplasm accession. The size of the included intron ranged from 89 to 858 nucleotides. Forty-eight unique markers were distinguished among 24 elite inbred lines, six partially isogenic inbred lines, nine open-pollinated populations, four Native American land races, and 20 wild H. annuus populations. Nine haplotypes (based on 24 RGCs) were identified among elite inbred lines and were correlated with known downy mildew resistance specificities. Sixteen out of 39 RGCs identified in wild H. annuus populations were not observed in elite germplasm. Five partially isogenic downy mildew resistant lines developed from wild H. annuus and H. praecox donors carried eight RGCs not found in other elite inbred lines. Twenty-four HaRGC1 loci were mapped to a 2-4 cM segment of linkage group 8. The multilocus IFLP marker and duplicated, hypervariable microsatellite markers tightly linked to the HaRGC1 cluster are powerful tools for distinguishing downy mildew resistance gene specificities and identifying and introgressing new downy mildew resistance gene specificities from wild sunflowers.
journal_name
Plant Biotechnol Jjournal_title
Plant biotechnology journalauthors
Slabaugh MB,Yu JK,Tang S,Heesacker A,Hu X,Lu G,Bidney D,Han F,Knapp SJdoi
10.1046/j.1467-7652.2003.00016.xsubject
Has Abstractpub_date
2003-05-01 00:00:00pages
167-85issue
3eissn
1467-7644issn
1467-7652pii
PBI016journal_volume
1pub_type
杂志文章abstract::Genetically engineered (GE) rice lines expressing Lepidoptera-active insecticidal cry genes from the bacterium Bacillus thuringiensis (Bt) have been developed in China. Field surveys indicated that Bt rice harbours fewer rice planthoppers than non-Bt rice although planthoppers are not sensitive to the produced Bt Cry ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12911
更新日期:2018-10-01 00:00:00
abstract::Recent developments of tools for targeted genome modification have led to new concepts in how multiple traits can be combined. Targeted genome modification is based on the use of nucleases with tailor-made specificities to introduce a DNA double-strand break (DSB) at specific target loci. A re-engineered meganuclease ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12085
更新日期:2013-10-01 00:00:00
abstract::Drought stress conditions decrease maize growth and yield, and aggravate preharvest aflatoxin contamination. While several studies have been performed on mature kernels responding to drought stress, the metabolic profiles of developing kernels are not as well characterized, particularly in germplasm with contrasting r...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12899
更新日期:2018-02-12 00:00:00
abstract::Salinity and drought are major environmental factors limiting the growth and productivity of alfalfa worldwide as this economically important legume forage is sensitive to these kinds of abiotic stress. In this study, transgenic alfalfa lines expressing both tonoplast NXH and H(+)-PPase genes, ZxNHX and ZxVP1-1 from t...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12451
更新日期:2016-03-01 00:00:00
abstract::Salicylic acid (SA) is an essential hormone for plant defence and development. SA perception is usually measured by counting the number of pathogens that grow in planta upon an exogenous application of the hormone. A biological SA perception model based on plant fresh weight reduction caused by disease resistance in A...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2009.00468.x
更新日期:2010-02-01 00:00:00
abstract::Aphids produce gel saliva during feeding which forms a sheath around the stylet as it penetrates through the apoplast. The sheath is required for the sustained ingestion of phloem sap from sieve elements and is thought to form when the structural sheath protein (SHP) is cross-linked by intermolecular disulphide bridge...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12322
更新日期:2015-08-01 00:00:00
abstract::Glycolate is produced in autotrophic cells under high temperatures and Ci -limitation via oxygenation of ribulose-1,5-bisphosphate. In unicellular algae, glycolate is lost via excretion or metabolized via the C2 cycle by consuming reductants, ATP and CO2 emission (photorespiration). Therefore, photorespiration is an i...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13078
更新日期:2019-08-01 00:00:00
abstract::Direct control of protein level enables rapid and efficient analyses of gene functions in crops. Previously, we developed the RDDK-Shield1 (Shld1) system in the model plant Arabidopsis thaliana for direct modulation of protein stabilization using a synthetic small molecule. However, it was unclear whether this system ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12787
更新日期:2018-02-01 00:00:00
abstract::Clustered regularly interspaced short palindromic repeat (CRISPR) and Cas9-associated protein systems provide a powerful genetic manipulation tool that can drive plant research forward. Nuclease-dead Cas9 (dCas9) is an enzymatically inactive mutant of Cas9 in which its endonuclease activity is non-functional. The appl...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/pbi.13232
更新日期:2020-01-01 00:00:00
abstract::Plants offer fast, flexible and easily scalable alternative platforms for the production of pharmaceutical proteins, but differences between plant and mammalian N-linked glycans, including the presence of β-1,2-xylose and core α-1,3-fucose residues in plants, can affect the activity, potency and immunogenicity of plan...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12981
更新日期:2019-02-01 00:00:00
abstract::Transgenic Bt rice expressing the insecticidal proteins derived from Bacillus thuringiensis Berliner (Bt) has been developed since 1989. Their ecological risks towards non-target organisms have been investigated; however, these studies were conducted individually, yielding uncertainty regarding potential agroecologica...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,meta分析
doi:10.1111/pbi.12698
更新日期:2017-08-01 00:00:00
abstract::Lutein and zeaxanthin cannot be synthesized de novo in humans, and although lutein is abundant in fruit and vegetables, good dietary sources of zeaxanthin are scarce. Certain corn varieties provide adequate amounts because the ratio of endosperm β:ε lycopene cyclase activity favours the β-carotene/zeaxanthin branch of...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2010.00554.x
更新日期:2011-04-01 00:00:00
abstract::Peanut allergy is an IgE-mediated adverse reaction to a subset of proteins found in peanuts. Immunotherapy aims to desensitize allergic patients through repeated and escalating exposures for several months to years using extracts or flours. The complex mix of proteins and variability between preparations complicates i...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12515
更新日期:2016-07-01 00:00:00
abstract::Bacterial wilt, caused by Ralstonia solanacearum, is a devastating disease affecting over 350 plant species. A few peanut cultivars were found to possess stable and durable bacterial wilt resistance (BWR). Genomics-assisted breeding can accelerate the process of developing resistant cultivars by using diagnostic marke...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13153
更新日期:2019-12-01 00:00:00
abstract::The capacity of the bread wheat (Triticum aestivum) genome to tolerate introgression from related genomes can be exploited for wheat improvement. A resistance to powdery mildew expressed by a derivative of the cross-bread wheat cv. Tähti × T. militinae (Tm) is known to be due to the incorporation of a Tm segment into ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12610
更新日期:2017-02-01 00:00:00
abstract::As an increasing number of genome sequences become available for a wide range of species, there is a growing understanding that the genome of a single individual is insufficient to represent the gene diversity within a whole species. Many studies examine the sequence diversity within genes, and this allelic variation ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/pbi.12499
更新日期:2016-04-01 00:00:00
abstract::In this study, the effects of the accumulation of cysteine protease inhibitors on the food preferences of adult female western flower thrips, Frankliniella occidentalis (Pergande), were investigated. Representative members of the cystatin and thyropin gene families (stefin A, cystatin C, kininogen domain 3 and equista...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2004.00088.x
更新日期:2004-09-01 00:00:00
abstract::The nitrate (NO3-) transporter has been selected as an important gene maker in the process of environmental adoption in rice cultivars. In this work, we transferred another native OsNAR2.1 promoter with driving OsNAR2.1 gene into rice plants. The transgenic lines with exogenous pOsNAR2.1:OsNAR2.1 constructs showed enh...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12714
更新日期:2017-10-01 00:00:00
abstract::Cadmium (Cd) is a widespread soil contaminant threatening human health. As an ideal energy plant, sweet sorghum (Sorghum bicolor (L.) Moench) has great potential in phytoremediation of Cd-polluted soils, although the molecular mechanisms are largely unknown. In this study, key factors responsible for differential Cd a...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12795
更新日期:2018-02-01 00:00:00
abstract::Dengue fever is a mosquito (Aedes aegypti) -transmitted viral disease that is endemic in more than 125 countries around the world. There are four serotypes of the dengue virus (DENV 1-4) and a safe and effective dengue vaccine must provide protection against all four serotypes. To date, the first vaccine, Dengvaxia (C...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13065
更新日期:2019-07-01 00:00:00
abstract::Single-chain variable fragments (scFvs), as well as other recombinant antibodies, have become powerful tools for therapy and analysis in both human and veterinary medicine. Transgenic plants have been developed as an efficient production system for these recombinant proteins, achieving high yields at moderate cost. Se...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2005.00176.x
更新日期:2006-03-01 00:00:00
abstract::Single-stranded oligonucleotides (ssODNs) can be used to direct the exchange of a single nucleotide or the repair of a single base within the coding region of a gene in a process that is known, generically, as gene editing. These molecules are composed of either all DNA residues or a mixture of RNA and DNA bases and u...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/pbi.12473
更新日期:2016-02-01 00:00:00
abstract::Antioxidants are protective molecules that neutralize reactive oxygen species and prevent oxidative damage to cellular components such as membranes, proteins and nucleic acids, therefore reducing the rate of cell death and hence the effects of ageing and ageing-related diseases. The fortification of food with antioxid...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/j.1467-7652.2012.00740.x
更新日期:2013-02-01 00:00:00
abstract::Genotype specificity is a big problem lagging the development of efficient hexaploid wheat transformation system. Increasingly, the biosecurity of genetically modified organisms is garnering public attention, so the generation of marker-free transgenic plants is very important to the eventual potential commercial rele...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12660
更新日期:2017-05-01 00:00:00
abstract::Burley tobaccos (Nicotiana tabacum) display a nitrogen-use-deficiency phenotype that is associated with the accumulation of high levels of nitrate within the leaf, a trait correlated with production of a class of compounds referred to as tobacco-specific nitrosamines (TSNAs). Two TSNA species, 4-(methylnitrosamino)-1-...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12510
更新日期:2016-07-01 00:00:00
abstract::Orobanche spp. (broomrape) are parasitic plants which subsist on the roots of a wide range of hosts, including tomato, causing severe losses in yield quality and quantity. Large amounts of mannitol accumulate in this parasitic weed during development. Mannose 6-phosphate reductase (M6PR) is a key enzyme in mannitol bi...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2009.00418.x
更新日期:2009-08-01 00:00:00
abstract::The related A genome species of the Oryza genus are the effective gene pool for rice. Here, we report draft genomes for two Australian wild A genome taxa: O. rufipogon-like population, referred to as Taxon A, and O. meridionalis-like population, referred to as Taxon B. These two taxa were sequenced and assembled by in...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12674
更新日期:2017-06-01 00:00:00
abstract::Abscisic acid (ABA) regulates plant adaptive responses to various environmental stresses, while L-ascorbic acid (AsA) that is also named vitamin C is an important antioxidant and involves in plant stress tolerance and the immune system in domestic animals. Transgenic tobacco (Nicotiana tabacum L.) and stylo [Stylosant...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12374
更新日期:2016-01-01 00:00:00
abstract::The advent of genetically modified crops in the late 1980s triggered a regulatory response to the relatively new field of plant genetic engineering. Over a 7-year period, a new regulatory framework was created, based on scientific principles that focused on risk mitigation. The process was transparent and deliberately...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/j.1467-7652.2007.00309.x
更新日期:2008-04-01 00:00:00
abstract::Whole-genome sequencing-based bulked segregant analysis (BSA) for mapping quantitative trait loci (QTL) provides an efficient alternative approach to conventional QTL analysis as it significantly reduces the scale and cost of analysis with comparable power to QTL detection using full mapping population. We tested the ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12964
更新日期:2019-01-01 00:00:00