Abstract:
:Drought stress conditions decrease maize growth and yield, and aggravate preharvest aflatoxin contamination. While several studies have been performed on mature kernels responding to drought stress, the metabolic profiles of developing kernels are not as well characterized, particularly in germplasm with contrasting resistance to both drought and mycotoxin contamination. Here, following screening for drought tolerance, a drought-sensitive line, B73, and a drought-tolerant line, Lo964, were selected and stressed beginning at 14 days after pollination. Developing kernels were sampled 7 and 14 days after drought induction (DAI) from both stressed and irrigated plants. Comparative biochemical and metabolomic analyses profiled 409 differentially accumulated metabolites. Multivariate statistics and pathway analyses showed that drought stress induced an accumulation of simple sugars and polyunsaturated fatty acids and a decrease in amines, polyamines and dipeptides in B73. Conversely, sphingolipid, sterol, phenylpropanoid and dipeptide metabolites accumulated in Lo964 under drought stress. Drought stress also resulted in the greater accumulation of reactive oxygen species (ROS) and aflatoxin in kernels of B73 in comparison with Lo964 implying a correlation in their production. Overall, field drought treatments disordered a cascade of normal metabolic programming during development of maize kernels and subsequently caused oxidative stress. The glutathione and urea cycles along with the metabolism of carbohydrates and lipids for osmoprotection, membrane maintenance and antioxidant protection were central among the drought stress responses observed in developing kernels. These results also provide novel targets to enhance host drought tolerance and disease resistance through the use of biotechnologies such as transgenics and genome editing.
journal_name
Plant Biotechnol Jjournal_title
Plant biotechnology journalauthors
Yang L,Fountain JC,Ji P,Ni X,Chen S,Lee RD,Kemerait RC,Guo Bdoi
10.1111/pbi.12899subject
Has Abstractpub_date
2018-02-12 00:00:00eissn
1467-7644issn
1467-7652pub_type
杂志文章abstract::Maize kernels do not contain enough of the essential sulphur-amino acid methionine (Met) to serve as a complete diet for animals, even though maize has the genetic capacity to store Met in kernels. Prior studies indicated that the availability of the sulphur (S)-amino acids may limit their incorporation into seed stor...
journal_title:Plant biotechnology journal
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journal_title:Plant biotechnology journal
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journal_title:Plant biotechnology journal
pub_type: 杂志文章
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更新日期:2016-07-01 00:00:00
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journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2008.00378.x
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pub_type: 杂志文章,评审
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更新日期:2014-12-01 00:00:00
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journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13106
更新日期:2019-10-01 00:00:00
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journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13241
更新日期:2020-03-01 00:00:00
abstract::Developing new strategies for crop plants to respond to drought is crucial for their innovative breeding. The down-regulation of nuclear cap-binding proteins in Arabidopsis renders plants drought tolerant. The CBP80 gene in the potato cultivar Desiree was silenced using artificial microRNAs. Transgenic plants displaye...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
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更新日期:2013-05-01 00:00:00
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journal_title:Plant biotechnology journal
pub_type: 杂志文章
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更新日期:2011-01-01 00:00:00
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journal_title:Plant biotechnology journal
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abstract::N-glycosylation is critical for recombinant glycoprotein production as it influences the heterogeneity of products and affects their biological function. In most eukaryotes, the oligosaccharyltransferase is the central-protein complex facilitating the N-glycosylation of proteins in the lumen of the endoplasmic reticul...
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abstract::The intensification of agriculture and the development of synthetic insecticides enabled worldwide grain production to more than double in the last third of the 20th century. However, the heavy dependence and, in some cases, overuse of insecticides has been responsible for negative environmental and ecological impacts...
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pub_type: 杂志文章,评审
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abstract::Transgenic rice expressing soybean glycinin in its endosperm was crossed with two types of low-glutelin mutants to determine how much storage the protein mutants can contribute to increases in glycinin accumulation. The glycinin level (102 microg/100 mg seed) in the parental transgenic line was enhanced to approximate...
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abstract::A dual-marker plasmid containing the selectable marker gene, manA, and the reporter gene, sgfp, was used to transform immature sorghum embryos by employing an Agrobacterium-mediated system. Both genes were under the control of the ubi1 promoter in a binary vector pPZP201. The Escherichia coli phosphomannose isomerase ...
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更新日期:2005-11-01 00:00:00