Glycolate from microalgae: an efficient carbon source for biotechnological applications.

abstract：:Dioxin residues widely contaminate soil and agricultural products at low concentrations and may accumulate in organisms at the top of food chains owing to their physicochemical properties. In this study, we have developed novel, dioxin-inducible, reporter gene expression systems regulated by recombinant aryl hydrocarb...

journal_title：Plant biotechnology journal

authors： Kodama S,Okada K,Akimoto K,Inui H,Ohkawa H

更新日期：2009-02-01 00:00:00

abstract：:We describe the site-directed integration (SDI) system for Agrobacterium-mediated transformation to precisely integrate a single copy of a desired gene into a predefined target locus by recombinase-mediated cassette exchange (RMCE). The system requires the selection of a transformed line with an integrated copy of a t...

journal_title：Plant biotechnology journal

authors： Nanto K,Yamada-Watanabe K,Ebinuma H

更新日期：2005-03-01 00:00:00

abstract：:Recent developments of tools for targeted genome modification have led to new concepts in how multiple traits can be combined. Targeted genome modification is based on the use of nucleases with tailor-made specificities to introduce a DNA double-strand break (DSB) at specific target loci. A re-engineered meganuclease ...

journal_title：Plant biotechnology journal

authors： D'Halluin K,Vanderstraeten C,Van Hulle J,Rosolowska J,Van Den Brande I,Pennewaert A,D'Hont K,Bossut M,Jantz D,Ruiter R,Broadhvest J

更新日期：2013-10-01 00:00:00

abstract：:Protein microbicides containing neutralizing antibodies and antiviral lectins may help to reduce the rate of infection with human immunodeficiency virus (HIV) if it is possible to manufacture the components in large quantities at a cost affordable in HIV-endemic regions such as sub-Saharan Africa. We expressed the ant...

journal_title：Plant biotechnology journal

authors： Vamvaka E,Arcalis E,Ramessar K,Evans A,O'Keefe BR,Shattock RJ,Medina V,Stöger E,Christou P,Capell T

更新日期：2016-06-01 00:00:00

abstract：:Methods based on single nucleotide polymorphism (SNP), copy number variation (CNV) and presence/absence variation (PAV) discovery provide a valuable resource to study gene structure and evolution. However, as a result of these structural variations, a single reference genome is unable to cover the entire gene content ...

journal_title：Plant biotechnology journal

authors： Dolatabadian A,Bayer PE,Tirnaz S,Hurgobin B,Edwards D,Batley J

更新日期：2020-04-01 00:00:00

abstract：:The hexaploid wheat (Triticum aestivum) adult plant resistance gene, Lr34/Yr18/Sr57/Pm38/Ltn1, provides broad-spectrum resistance to wheat leaf rust (Lr34), stripe rust (Yr18), stem rust (Sr57) and powdery mildew (Pm38) pathogens, and has remained effective in wheat crops for many decades. The partial resistance provi...

journal_title：Plant biotechnology journal

authors： Rinaldo A,Gilbert B,Boni R,Krattinger SG,Singh D,Park RF,Lagudah E,Ayliffe M

更新日期：2017-07-01 00:00:00

abstract：:Genetic modification of Zn/Cd accumulation in roots and shoots for biofortification or phytoremediation is a focus of this manuscript. We expressed AtHMA4 (a P(₁B) ATPase involved in Zn and Cd transport), AtHMA4-trunc (lacking the C-terminal region) and AtHMA4-C terminus (the C-terminal region alone) in tobacco under ...

journal_title：Plant biotechnology journal

authors： Siemianowski O,Mills RF,Williams LE,Antosiewicz DM

更新日期：2011-01-01 00:00:00

abstract：:Suppression of the microsomal ω6 oleate desaturase during the seed development of soybean (Glycine max) with the 420-bp soybean FAD2-1A intron as RNAi trigger shifts the conventional fatty acid composition of soybean oil from 20% oleic and 60% polyunsaturates to one containing greater than 80% oleic acid and less than...

journal_title：Plant biotechnology journal

authors： Wagner N,Mroczka A,Roberts PD,Schreckengost W,Voelker T

更新日期：2011-09-01 00:00:00

abstract：:Heavy metal accumulation in the environment poses great risks to flora and fauna. However, monitoring sites prone to accumulation poses scale and economic challenges. In this study, we present and test a method for monitoring these sites using fluorescent resonance energy transfer (FRET) change in response to zinc (Zn...

journal_title：Plant biotechnology journal

authors： Adams JP,Adeli A,Hsu CY,Harkess RL,Page GP,Depamphilis CW,Schultz EB,Yuceer C

更新日期：2012-02-01 00:00:00

abstract：:Transgenic Bt rice expressing the insecticidal proteins derived from Bacillus thuringiensis Berliner (Bt) has been developed since 1989. Their ecological risks towards non-target organisms have been investigated; however, these studies were conducted individually, yielding uncertainty regarding potential agroecologica...

journal_title：Plant biotechnology journal

authors： Dang C,Lu Z,Wang L,Chang X,Wang F,Yao H,Peng Y,Stanley D,Ye G

更新日期：2017-08-01 00:00:00

abstract：:Sugarcane (Saccharum spp.) is a highly energy-efficient crop primarily for sugar and bio-ethanol production. Sugarcane genetics and cultivar improvement have been extremely challenging largely due to its complex genomes with high polyploidy levels. In this study, we deeply sequenced the coding regions of 307 sugarcane...

journal_title：Plant biotechnology journal

authors： Yang X,Song J,Todd J,Peng Z,Paudel D,Luo Z,Ma X,You Q,Hanson E,Zhao Z,Zhao Y,Zhang J,Ming R,Wang J

更新日期：2019-02-01 00:00:00

abstract：:Co-expression of protease inhibitors like the tomato cystatin SlCYS8 is useful to increase recombinant protein production in plants, but key proteases involved in protein proteolysis are still unknown. Here, we performed activity-based protein profiling to identify proteases that are inhibited by SlCYS8 in agroinfiltr...

journal_title：Plant biotechnology journal

authors： Jutras PV,Grosse-Holz F,Kaschani F,Kaiser M,Michaud D,van der Hoorn RAL

更新日期：2019-08-01 00:00:00

abstract：:Despite the declarations and collective measures taken to eradicate hunger at World Food Summits, food security remains one of the biggest issues that we are faced with. The current scenario could worsen due to the alarming increase in world population, further compounded by adverse climatic conditions, such as increa...

journal_title：Plant biotechnology journal

authors： Singh J,Pandey P,James D,Chandrasekhar K,Achary VM,Kaul T,Tripathy BC,Reddy MK

更新日期：2014-12-01 00:00:00

abstract：:S-adenosyl-L-methionine (SAM) synthetase is the key enzyme involved in the biosynthesis of SAM, which serves as a common precursor for polyamines (PAs) and ethylene. A SAM synthetase cDNA (SlSAMS1) was introduced into the tomato genome using the Agrobacterium tumefaciens transformation method. Transgenic plants overex...

journal_title：Plant biotechnology journal

authors： Gong B,Li X,VandenLangenberg KM,Wen D,Sun S,Wei M,Li Y,Yang F,Shi Q,Wang X

更新日期：2014-08-01 00:00:00

abstract：:Phytochrome-interacting factors (PIFs) play major roles in regulating plant growth and development, but their roles in drought stress remain elusive. Here, we cloned and characterized a maize (Zea mays) PIF transcription factor, ZmPIF1. The expression level of ZmPIF1 was significantly induced by independent drought an...

journal_title：Plant biotechnology journal

authors： Gao Y,Wu M,Zhang M,Jiang W,Ren X,Liang E,Zhang D,Zhang C,Xiao N,Li Y,Dai Y,Chen J

更新日期：2018-07-01 00:00:00

abstract：:Over 3.5 million expressed sequence tags from the major cereal taxa were used to electronically mine over 176,000 putative single nucleotide polymorphisms (SNPs). The density, distribution and degree of linkage between these SNPs were compared among the different taxa. The frequency of sequence polymorphism was lowest...

journal_title：Plant biotechnology journal

authors： Barker GL,Edwards KJ

更新日期：2009-05-01 00:00:00

abstract：:Developing new strategies for crop plants to respond to drought is crucial for their innovative breeding. The down-regulation of nuclear cap-binding proteins in Arabidopsis renders plants drought tolerant. The CBP80 gene in the potato cultivar Desiree was silenced using artificial microRNAs. Transgenic plants displaye...

journal_title：Plant biotechnology journal

authors： Pieczynski M,Marczewski W,Hennig J,Dolata J,Bielewicz D,Piontek P,Wyrzykowska A,Krusiewicz D,Strzelczyk-Zyta D,Konopka-Postupolska D,Krzeslowska M,Jarmolowski A,Szweykowska-Kulinska Z

更新日期：2013-05-01 00:00:00

abstract：:Threonine (Thr) is one of a few limiting essential amino acids (EAAs) in the animal feed industry, and its level in feed rations can impact production of important meat sources, such as swine and poultry. Threonine as well as EAAs lysine (Lys) and methionine (Met) are all synthesized via the aspartate family pathway. ...

journal_title：Plant biotechnology journal

authors： Qi Q,Huang J,Crowley J,Ruschke L,Goldman BS,Wen L,Rapp WD

更新日期：2011-02-01 00:00:00

abstract：:The development and adoption of hybrid seed technology have led to dramatic increases in agricultural productivity. However, it has been a challenge to develop a commercially viable platform for the production of hybrid wheat (Triticum aestivum) seed due to wheat's strong inbreeding habit. Recently, a novel platform f...

journal_title：Plant biotechnology journal

authors： Okada A,Arndell T,Borisjuk N,Sharma N,Watson-Haigh NS,Tucker EJ,Baumann U,Langridge P,Whitford R

更新日期：2019-10-01 00:00:00

abstract：:The cooking quality of rice is associated with the starch gelatinization temperature (GT). Rice genotypes with low GT have probably been selected for their cooking quality by humans during domestication. We now report polymorphisms in starch synthase IIa (SSIIa) that explain the variation in rice starch GT. Sequence a...

journal_title：Plant biotechnology journal

authors： Waters DL,Henry RJ,Reinke RF,Fitzgerald MA

更新日期：2006-01-01 00:00:00

abstract：:Mutation discovery technologies have enabled the development of reverse genetics for many plant species and allowed sophisticated evaluation of the consequences of mutagenesis. Such methods are relatively straightforward for seed-propagated plants. To develop a platform suitable for vegetatively propagated species, we...

journal_title：Plant biotechnology journal

authors： Jankowicz-Cieslak J,Huynh OA,Brozynska M,Nakitandwe J,Till BJ

更新日期：2012-12-01 00:00:00

abstract：:Arsenic (As) is a poisonous element that causes severe skin lesions and cancer in humans. Rice (Oryza sativa L.) is a major dietary source of As in humans who consume this cereal as a staple food. We hypothesized that increasing As vacuolar sequestration would inhibit its translocation into the grain and reduce the am...

journal_title：Plant biotechnology journal

authors： Deng F,Yamaji N,Ma JF,Lee SK,Jeon JS,Martinoia E,Lee Y,Song WY

更新日期：2018-10-01 00:00:00

abstract：:With the aim of increasing the methionine level in alfalfa (Medicago sativa L.) and thus improving its nutritional quality, we produced transgenic alfalfa plants that expressed the Arabidopsis cystathionine gamma-synthase (AtCGS), the enzyme that controls the synthesis of the first intermediate metabolite in the methi...

journal_title：Plant biotechnology journal

authors： Avraham T,Badani H,Galili S,Amir R

更新日期：2005-01-01 00:00:00

abstract：:Nanobodies® (VHHs) provide powerful tools in therapeutic and biotechnological applications. Nevertheless, for some applications, bivalent antibodies perform much better, and for this, an Fc chain can be fused to the VHH domain, resulting in a bivalent homodimeric VHH-Fc complex. However, the production of bivalent ant...

journal_title：Plant biotechnology journal

authors： De Buck S,Nolf J,De Meyer T,Virdi V,De Wilde K,Van Lerberge E,Van Droogenbroeck B,Depicker A

更新日期：2013-10-01 00:00:00

abstract：:Expression of industrial enzymes in transgenic plants offers an alternative system to fungal fermentation for large-scale production. Very high levels of expression are required to make the enzymes cost-effective. We tested several parameters to determine the best method for achieving high levels of expression for a f...

journal_title：Plant biotechnology journal

authors： Hood EE,Bailey MR,Beifuss K,Magallanes-Lundback M,Horn ME,Callaway E,Drees C,Delaney DE,Clough R,Howard JA

更新日期：2003-03-01 00:00:00

abstract：:Food security is a global concern and substantial yield increases in cereal crops are required to feed the growing world population. Wheat is one of the three most important crops for human and livestock feed. However, the complexity of the genome coupled with a decline in genetic diversity within modern elite cultiva...

journal_title：Plant biotechnology journal

authors： Allen AM,Barker GL,Berry ST,Coghill JA,Gwilliam R,Kirby S,Robinson P,Brenchley RC,D'Amore R,McKenzie N,Waite D,Hall A,Bevan M,Hall N,Edwards KJ

更新日期：2011-12-01 00:00:00

abstract：:Willows (Salix spp.) are important biomass crops due to their ability to grow rapidly with low fertilizer inputs and ease of cultivation in short-rotation coppice cycles. They are relatively undomesticated and highly diverse, but functional testing to identify useful allelic variation is time-consuming in trees and tr...

journal_title：Plant biotechnology journal

authors： Salmon J,Ward SP,Hanley SJ,Leyser O,Karp A

更新日期：2014-05-01 00:00:00

abstract：:Recently, CRISPR-Cas12a (Cpf1) from Prevotella and Francisella was engineered to modify plant genomes. In this report, we employed CRISPR-LbCas12a (LbCpf1), which is derived from Lachnospiraceae bacterium ND2006, to edit a citrus genome for the first time. First, LbCas12a was used to modify the CsPDS gene successfully...

journal_title：Plant biotechnology journal

authors： Jia H,Orbović V,Wang N

更新日期：2019-10-01 00:00:00

abstract：:Transcription factors have been shown to control the activity of multiple stress response genes in a coordinated manner and therefore represent attractive targets for application in molecular plant breeding. We investigated the possibility of modulating the transcriptional regulation of drought and cold responses in t...

journal_title：Plant biotechnology journal

authors： Morran S,Eini O,Pyvovarenko T,Parent B,Singh R,Ismagul A,Eliby S,Shirley N,Langridge P,Lopato S

更新日期：2011-02-01 00:00:00

abstract：:The replacement of crude allergen extracts by selected allergens currently represents a major goal for the improvement of allergy diagnosis and immunotherapy. Indeed, the development of molecularly defined vaccines would facilitate both standardization and enhance batch-to-batch reproducibility as well as treatment sp...

journal_title：Plant biotechnology journal

authors： Lienard D,Tran Dinh O,van Oort E,Van Overtvelt L,Bonneau C,Wambre E,Bardor M,Cosette P,Didier-Laurent A,de Borne FD,Delon R,van Ree R,Moingeon P,Faye L,Gomord V

更新日期：2007-01-01 00:00:00