Activity-based proteomics reveals nine target proteases for the recombinant protein-stabilizing inhibitor SlCYS8 in Nicotiana benthamiana.

abstract：:Transcription factors have been shown to control the activity of multiple stress response genes in a coordinated manner and therefore represent attractive targets for application in molecular plant breeding. We investigated the possibility of modulating the transcriptional regulation of drought and cold responses in t...

journal_title：Plant biotechnology journal

authors： Morran S,Eini O,Pyvovarenko T,Parent B,Singh R,Ismagul A,Eliby S,Shirley N,Langridge P,Lopato S

更新日期：2011-02-01 00:00:00

abstract：:Recently, CRISPR-Cas12a (Cpf1) from Prevotella and Francisella was engineered to modify plant genomes. In this report, we employed CRISPR-LbCas12a (LbCpf1), which is derived from Lachnospiraceae bacterium ND2006, to edit a citrus genome for the first time. First, LbCas12a was used to modify the CsPDS gene successfully...

journal_title：Plant biotechnology journal

authors： Jia H,Orbović V,Wang N

更新日期：2019-10-01 00:00:00

abstract：:Approximately 20,000 of the rice-FOX Arabidopsis transgenic lines, which overexpress 13,000 rice full-length cDNAs at random in Arabidopsis, were screened for bacterial disease resistance by dip inoculation with Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). The identities of the overexpressed genes were determi...

journal_title：Plant biotechnology journal

authors： Dubouzet JG,Maeda S,Sugano S,Ohtake M,Hayashi N,Ichikawa T,Kondou Y,Kuroda H,Horii Y,Matsui M,Oda K,Hirochika H,Takatsuji H,Mori M

更新日期：2011-05-01 00:00:00

abstract：:Salicylic acid (SA) is an essential hormone for plant defence and development. SA perception is usually measured by counting the number of pathogens that grow in planta upon an exogenous application of the hormone. A biological SA perception model based on plant fresh weight reduction caused by disease resistance in A...

journal_title：Plant biotechnology journal

authors： Canet JV,Dobón A,Ibáñez F,Perales L,Tornero P

更新日期：2010-02-01 00:00:00

abstract：:As an increasing number of genome sequences become available for a wide range of species, there is a growing understanding that the genome of a single individual is insufficient to represent the gene diversity within a whole species. Many studies examine the sequence diversity within genes, and this allelic variation ...

journal_title：Plant biotechnology journal

authors： Golicz AA,Batley J,Edwards D

更新日期：2016-04-01 00:00:00

abstract：:Genetic modification of Zn/Cd accumulation in roots and shoots for biofortification or phytoremediation is a focus of this manuscript. We expressed AtHMA4 (a P(₁B) ATPase involved in Zn and Cd transport), AtHMA4-trunc (lacking the C-terminal region) and AtHMA4-C terminus (the C-terminal region alone) in tobacco under ...

journal_title：Plant biotechnology journal

authors： Siemianowski O,Mills RF,Williams LE,Antosiewicz DM

更新日期：2011-01-01 00:00:00

abstract：:Burley tobaccos (Nicotiana tabacum) display a nitrogen-use-deficiency phenotype that is associated with the accumulation of high levels of nitrate within the leaf, a trait correlated with production of a class of compounds referred to as tobacco-specific nitrosamines (TSNAs). Two TSNA species, 4-(methylnitrosamino)-1-...

journal_title：Plant biotechnology journal

authors： Lu J,Zhang L,Lewis RS,Bovet L,Goepfert S,Jack AM,Crutchfield JD,Ji H,Dewey RE

更新日期：2016-07-01 00:00:00

abstract：:Reverse transcription-polymerase chain reaction (RT-PCR) approaches have been used in a large proportion of transcriptome analyses published to date. The accuracy of the results obtained by this method strongly depends on accurate transcript normalization using stably expressed genes, known as references. Statistical ...

journal_title：Plant biotechnology journal

authors： Gutierrez L,Mauriat M,Guénin S,Pelloux J,Lefebvre JF,Louvet R,Rusterucci C,Moritz T,Guerineau F,Bellini C,Van Wuytswinkel O

更新日期：2008-08-01 00:00:00

abstract：:One goal of modern agriculture is the improvement of plant drought tolerance and water-use efficiency (WUE). Although stomatal density has been linked to WUE, the causal molecular mechanisms and engineered alternations of this relationship are not yet fully understood. Moreover, YODA (YDA), which is a MAPKK kinase gen...

journal_title：Plant biotechnology journal

authors： Meng LS,Yao SQ

更新日期：2015-09-01 00:00:00

abstract：:The plant immune system involves detection of pathogens via both cell-surface and intracellular receptors. Both receptor classes can induce transcriptional reprogramming that elevates disease resistance. To assess differential gene expression during plant immunity, we developed and deployed quantitative sequence captu...

journal_title：Plant biotechnology journal

authors： Ding P,Ngou BPM,Furzer OJ,Sakai T,Shrestha RK,MacLean D,Jones JDG

更新日期：2020-07-01 00:00:00

abstract：:There is a pressing need to develop novel antibacterial agents given the widespread antibiotic resistance among pathogenic bacteria and the low specificity of the drugs available. Endolysins are antibacterial proteins that are produced by bacteriophage-infected cells to digest the bacterial cell wall for phage progeny...

journal_title：Plant biotechnology journal

authors： Stoffels L,Taunt HN,Charalambous B,Purton S

更新日期：2017-09-01 00:00:00

abstract：:A high throughput proton nuclear magnetic resonance spectroscopy method for the metabolite fingerprinting of plants was applied to genetically modified peas (Pisum sativum) to determine whether biochemical changes, so called 'unintended effects', beyond those intended by incorporation of a transgene, were detectable. ...

journal_title：Plant biotechnology journal

authors： Charlton A,Allnutt T,Holmes S,Chisholm J,Bean S,Ellis N,Mullineaux P,Oehlschlager S

更新日期：2004-01-01 00:00:00

abstract：:Transgenic Bt rice expressing the insecticidal proteins derived from Bacillus thuringiensis Berliner (Bt) has been developed since 1989. Their ecological risks towards non-target organisms have been investigated; however, these studies were conducted individually, yielding uncertainty regarding potential agroecologica...

journal_title：Plant biotechnology journal

authors： Dang C,Lu Z,Wang L,Chang X,Wang F,Yao H,Peng Y,Stanley D,Ye G

更新日期：2017-08-01 00:00:00

abstract：:Production of novel transgenic floricultural crops with altered petal properties requires transgenes that confer a useful trait and petal-specific promoters. Several promoters have been shown to control transgenes in petals. However, all suffer from inherent drawbacks such as low petal specificity and restricted activ...

journal_title：Plant biotechnology journal

authors： Azuma M,Morimoto R,Hirose M,Morita Y,Hoshino A,Iida S,Oshima Y,Mitsuda N,Ohme-Takagi M,Shiratake K

更新日期：2016-01-01 00:00:00

abstract：:Interspecific genomic variation can provide a genetic basis for local adaptation and domestication. A series of studies have presented its role of interspecific haplotypes and introgressions in adaptive traits, but few studies have addressed their role in improving agronomic character. Two allotetraploid Gossypium spe...

journal_title：Plant biotechnology journal

authors： Fang L,Zhao T,Hu Y,Si Z,Zhu X,Han Z,Liu G,Wang S,Ju L,Guo M,Mei H,Wang L,Qi B,Wang H,Guan X,Zhang T

更新日期：2021-01-14 00:00:00

abstract：:MicroRNAs (miRNAs) are a class of regulatory small RNAs (sRNAs) that down-regulate target genes through mRNA cleavage or translational inhibition. miRNA is known to play an important role in the root development and environmental responses in both the Arabidopsis and rice. However, little information is available to f...

journal_title：Plant biotechnology journal

authors： Kong X,Zhang M,Xu X,Li X,Li C,Ding Z

更新日期：2014-10-01 00:00:00

abstract：:Drought conditions limit agricultural production by preventing crops from reaching their genetically predetermined maximum yields. Here, we present the results of field evaluations of rice overexpressing OsNAC9, a member of the rice NAC domain family. Root-specific (RCc3) and constitutive (GOS2) promoters were used to...

journal_title：Plant biotechnology journal

authors： Redillas MC,Jeong JS,Kim YS,Jung H,Bang SW,Choi YD,Ha SH,Reuzeau C,Kim JK

更新日期：2012-09-01 00:00:00

abstract：:The nitrate (NO3-) transporter has been selected as an important gene maker in the process of environmental adoption in rice cultivars. In this work, we transferred another native OsNAR2.1 promoter with driving OsNAR2.1 gene into rice plants. The transgenic lines with exogenous pOsNAR2.1:OsNAR2.1 constructs showed enh...

journal_title：Plant biotechnology journal

authors： Chen J,Fan X,Qian K,Zhang Y,Song M,Liu Y,Xu G,Fan X

更新日期：2017-10-01 00:00:00

abstract：:Although it is one of the major crops in the world, corn has poor nutritional quality for human and animal consumption due to its low lysine content. Here, we report a method of simultaneous expression of a deregulated lysine biosynthetic enzyme, CordapA, and reduction of a bifunctional lysine degradation enzyme, lysi...

journal_title：Plant biotechnology journal

authors： Frizzi A,Huang S,Gilbertson LA,Armstrong TA,Luethy MH,Malvar TM

更新日期：2008-01-01 00:00:00

abstract：:Abscisic acid (ABA) regulates plant adaptive responses to various environmental stresses, while L-ascorbic acid (AsA) that is also named vitamin C is an important antioxidant and involves in plant stress tolerance and the immune system in domestic animals. Transgenic tobacco (Nicotiana tabacum L.) and stylo [Stylosant...

journal_title：Plant biotechnology journal

authors： Bao G,Zhuo C,Qian C,Xiao T,Guo Z,Lu S

更新日期：2016-01-01 00:00:00

abstract：:Transgenic engineering of plants is important in both basic and applied research. However, the expression of a transgene can dwindle over time as the plant's small (s)RNA-guided silencing pathways shut it down. The silencing pathways have evolved as antiviral defence mechanisms, and viruses have co-evolved viral silen...

journal_title：Plant biotechnology journal

authors： Naim F,Shrestha P,Singh SP,Waterhouse PM,Wood CC

更新日期：2016-06-01 00:00:00

abstract：:Genome modification by homology-directed repair (HDR) is an attractive tool for the controlled genetic manipulation of plants. Here, we report the HDR-mediated gene exchange of expression cassettes in tobacco BY-2 cells using a designed zinc finger nuclease (ZFN). The target contained a 7-kb fragment flanked by two ZF...

journal_title：Plant biotechnology journal

authors： Schneider K,Schiermeyer A,Dolls A,Koch N,Herwartz D,Kirchhoff J,Fischer R,Russell SM,Cao Z,Corbin DR,Sastry-Dent L,Ainley WM,Webb SR,Schinkel H,Schillberg S

更新日期：2016-04-01 00:00:00

abstract：:Genetic improvement of fibre quality is one of the main breeding goals for the upland cotton, Gossypium hirsutum, but there are difficulties with precise selection of traits. Therefore, it is important to improve the understanding of the genetic basis of phenotypic variation. In this study, we conducted phenotyping an...

journal_title：Plant biotechnology journal

authors： Sun Z,Wang X,Liu Z,Gu Q,Zhang Y,Li Z,Ke H,Yang J,Wu J,Wu L,Zhang G,Zhang C,Ma Z

更新日期：2017-08-01 00:00:00

abstract：:Menadione sodium bisulphite (MSB) is a water-soluble derivative of vitamin K3, or menadione, and has been previously demonstrated to function as a plant defence activator against several pathogens in several plant species. However, there are no reports of the role of this vitamin in the induction of resistance in the ...

journal_title：Plant biotechnology journal

authors： Borges AA,Dobon A,Expósito-Rodríguez M,Jiménez-Arias D,Borges-Pérez A,Casañas-Sánchez V,Pérez JA,Luis JC,Tornero P

更新日期：2009-10-01 00:00:00

abstract：:Tomato is one of the most cultivated vegetables in the world and an important ingredient of the human diet. Tomato breeders and growers face a continuous challenge of combining high quantity (production volume) with high quality (appearance, taste and perception for the consumers, processing quality for the processing...

journal_title：Plant biotechnology journal

authors： Czerednik A,Busscher M,Angenent GC,de Maagd RA

更新日期：2015-02-01 00:00:00

abstract：:Zinc-finger nucleases (ZFNs) are artificial restriction enzymes, custom designed for induction of double-strand breaks (DSBs) at a specific locus. These DSBs may result in site-specific mutagenesis or homologous recombination at the repair site, depending on the DNA repair pathway that is used. These promising techniq...

journal_title：Plant biotechnology journal

authors： de Pater S,Neuteboom LW,Pinas JE,Hooykaas PJ,van der Zaal BJ

更新日期：2009-10-01 00:00:00

abstract：:Expression of industrial enzymes in transgenic plants offers an alternative system to fungal fermentation for large-scale production. Very high levels of expression are required to make the enzymes cost-effective. We tested several parameters to determine the best method for achieving high levels of expression for a f...

journal_title：Plant biotechnology journal

authors： Hood EE,Bailey MR,Beifuss K,Magallanes-Lundback M,Horn ME,Callaway E,Drees C,Delaney DE,Clough R,Howard JA

更新日期：2003-03-01 00:00:00

abstract：:Squalene is a linear intermediate to nearly all classes of triterpenes and sterols and is itself highly valued for its use in wide range of industrial applications. Another unique linear triterpene is botryococcene and its methylated derivatives generated by the alga Botryococcus braunii race B, which are progenitors ...

journal_title：Plant biotechnology journal

authors： Jiang Z,Kempinski C,Kumar S,Kinison S,Linscott K,Nybo E,Janze S,Wood C,Chappell J

更新日期：2018-06-01 00:00:00

abstract：:Glyphosate is a popular, systemic, broad-spectrum herbicide used in modern agriculture. Being a structural analog of phosphoenolpyruvate (PEP), it inhibits 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) which is responsible for the biosynthesis of aromatic amino acids and various aromatic secondary metabolites. T...

journal_title：Plant biotechnology journal

authors： Achary VMM,Sheri V,Manna M,Panditi V,Borphukan B,Ram B,Agarwal A,Fartyal D,Teotia D,Masakapalli SK,Agrawal PK,Reddy MK

更新日期：2020-12-01 00:00:00

abstract：:Considered responsible for one million deaths in Ireland and widespread famine in the European continent during the 1840s, late blight, caused by Phytophthora infestans, remains the most devastating disease of potato (Solanum tuberosum L.) with about 15%-30% annual yield loss in sub-Saharan Africa, affecting mainly sm...

journal_title：Plant biotechnology journal

authors： Ghislain M,Byarugaba AA,Magembe E,Njoroge A,Rivera C,Román ML,Tovar JC,Gamboa S,Forbes GA,Kreuze JF,Barekye A,Kiggundu A

更新日期：2019-06-01 00:00:00