Agrobacterium-mediated RMCE approach for gene replacement.

abstract：:Mutation discovery technologies have enabled the development of reverse genetics for many plant species and allowed sophisticated evaluation of the consequences of mutagenesis. Such methods are relatively straightforward for seed-propagated plants. To develop a platform suitable for vegetatively propagated species, we...

journal_title：Plant biotechnology journal

authors： Jankowicz-Cieslak J,Huynh OA,Brozynska M,Nakitandwe J,Till BJ

更新日期：2012-12-01 00:00:00

abstract：:A new understanding of leaf starch degradation has emerged in the last 10 years. It has been shown that starch phosphorylation and dephosphorylation are critical components of this process. Glucan, water dikinase (GWD) (and phosphoglucan, water dikinase) adds phosphate to starch, and phosphoglucan phosphatase (SEX4) r...

journal_title：Plant biotechnology journal

authors： Weise SE,Aung K,Jarou ZJ,Mehrshahi P,Li Z,Hardy AC,Carr DJ,Sharkey TD

更新日期：2012-06-01 00:00:00

abstract：:Salicylic acid (SA) is an essential hormone for plant defence and development. SA perception is usually measured by counting the number of pathogens that grow in planta upon an exogenous application of the hormone. A biological SA perception model based on plant fresh weight reduction caused by disease resistance in A...

journal_title：Plant biotechnology journal

authors： Canet JV,Dobón A,Ibáñez F,Perales L,Tornero P

更新日期：2010-02-01 00:00:00

abstract：:Next-generation sequencing technologies provide opportunities to understand the genetic basis of phenotypic differences, such as abiotic stress response, even in the closely related cultivars via identification of large number of DNA polymorphisms. We performed whole-genome resequencing of three rice cultivars with co...

journal_title：Plant biotechnology journal

authors： Jain M,Moharana KC,Shankar R,Kumari R,Garg R

更新日期：2014-02-01 00:00:00

abstract：:A dual-marker plasmid containing the selectable marker gene, manA, and the reporter gene, sgfp, was used to transform immature sorghum embryos by employing an Agrobacterium-mediated system. Both genes were under the control of the ubi1 promoter in a binary vector pPZP201. The Escherichia coli phosphomannose isomerase ...

journal_title：Plant biotechnology journal

authors： Gao Z,Xie X,Ling Y,Muthukrishnan S,Liang GH

更新日期：2005-11-01 00:00:00

abstract：:Bacterial wilt, caused by Ralstonia solanacearum, is a devastating disease affecting over 350 plant species. A few peanut cultivars were found to possess stable and durable bacterial wilt resistance (BWR). Genomics-assisted breeding can accelerate the process of developing resistant cultivars by using diagnostic marke...

journal_title：Plant biotechnology journal

authors： Luo H,Pandey MK,Khan AW,Wu B,Guo J,Ren X,Zhou X,Chen Y,Chen W,Huang L,Liu N,Lei Y,Liao B,Varshney RK,Jiang H

更新日期：2019-12-01 00:00:00

abstract：:The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid...

journal_title：Plant biotechnology journal

authors： Tomlinson L,Yang Y,Emenecker R,Smoker M,Taylor J,Perkins S,Smith J,MacLean D,Olszewski NE,Jones JDG

更新日期：2019-01-01 00:00:00

abstract：:Broad spectrum protection against different insects and pathogens requires multigene engineering. However, such broad spectrum protection against biotic stress is provided by a single protein in some medicinal plants. Therefore, tobacco chloroplasts were transformed with the agglutinin gene from Pinellia ternata (pta)...

journal_title：Plant biotechnology journal

authors： Jin S,Zhang X,Daniell H

更新日期：2012-04-01 00:00:00

abstract：:The subspecies fastigiata of cultivated groundnut lost fresh seed dormancy (FSD) during domestication and human-made selection. Groundnut varieties lacking FSD experience precocious seed germination during harvest imposing severe losses. Development of easy-to-use genetic markers enables early-generation selection in ...

journal_title：Plant biotechnology journal

authors： Kumar R,Janila P,Vishwakarma MK,Khan AW,Manohar SS,Gangurde SS,Variath MT,Shasidhar Y,Pandey MK,Varshney RK

更新日期：2020-04-01 00:00:00

abstract：:Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer c...

journal_title：Plant biotechnology journal

authors： Ramírez-Estrada K,Altabella T,Onrubia M,Moyano E,Notredame C,Osuna L,Vanden Bossche R,Goossens A,Cusido RM,Palazon J

更新日期：2016-01-01 00:00:00

abstract：:Cadmium (Cd) is a widespread soil contaminant threatening human health. As an ideal energy plant, sweet sorghum (Sorghum bicolor (L.) Moench) has great potential in phytoremediation of Cd-polluted soils, although the molecular mechanisms are largely unknown. In this study, key factors responsible for differential Cd a...

journal_title：Plant biotechnology journal

authors： Feng J,Jia W,Lv S,Bao H,Miao F,Zhang X,Wang J,Li J,Li D,Zhu C,Li S,Li Y

更新日期：2018-02-01 00:00:00

abstract：:Soya bean (Glycine max) and grass pea (Lathyrus sativus) seeds are important sources of dietary proteins; however, they also contain antinutritional metabolite oxalic acid (OA). Excess dietary intake of OA leads to nephrolithiasis due to the formation of calcium oxalate crystals in kidneys. Besides, OA is also a known...

journal_title：Plant biotechnology journal

authors： Kumar V,Chattopadhyay A,Ghosh S,Irfan M,Chakraborty N,Chakraborty S,Datta A

更新日期：2016-06-01 00:00:00

abstract：:Expression of the cecropin A gene in rice confers resistance to the rice blast fungus Magnaporthe oryzae. In this study, a polymerase chain reaction-based suppression subtractive hybridization approach was used to generate a cDNA macroarray from the elite japonica rice (Oryza sativa L.) cultivar 'Senia'. Gene expressi...

journal_title：Plant biotechnology journal

authors： Campo S,Manrique S,García-Martínez J,San Segundo B

更新日期：2008-08-01 00:00:00

abstract：:Arsenic (As) is a poisonous element that causes severe skin lesions and cancer in humans. Rice (Oryza sativa L.) is a major dietary source of As in humans who consume this cereal as a staple food. We hypothesized that increasing As vacuolar sequestration would inhibit its translocation into the grain and reduce the am...

journal_title：Plant biotechnology journal

authors： Deng F,Yamaji N,Ma JF,Lee SK,Jeon JS,Martinoia E,Lee Y,Song WY

更新日期：2018-10-01 00:00:00

abstract：:Flavonoids are widely distributed in plants and play important roles in human and animal health and nutrition. Model plants with discernible flavonoid phenotypes, such as Arabidopsis seed patterning lines, are valuable tools that can provide avenues for understanding flavonoid and proanthocyanidin accumulation pattern...

journal_title：Plant biotechnology journal

authors： Gao P,Li X,Cui D,Wu L,Parkin I,Gruber MY

更新日期：2010-12-01 00:00:00

abstract：:Suppression of the microsomal ω6 oleate desaturase during the seed development of soybean (Glycine max) with the 420-bp soybean FAD2-1A intron as RNAi trigger shifts the conventional fatty acid composition of soybean oil from 20% oleic and 60% polyunsaturates to one containing greater than 80% oleic acid and less than...

journal_title：Plant biotechnology journal

authors： Wagner N,Mroczka A,Roberts PD,Schreckengost W,Voelker T

更新日期：2011-09-01 00:00:00

abstract：:We constructed a novel autonomously replicating gene expression shuttle vector, with the aim of developing a system for transiently expressing proteins at levels useful for commercial production of vaccines and other proteins in plants. The vector, pRIC, is based on the mild strain of the geminivirus Bean yellow dwarf...

journal_title：Plant biotechnology journal

authors： Regnard GL,Halley-Stott RP,Tanzer FL,Hitzeroth II,Rybicki EP

更新日期：2010-01-01 00:00:00

abstract：:Coordination of multiple transgenes is essential for metabolic engineering of biosynthetic pathways. Here, we report the utilization of two bicistronic systems involving the 2A sequence from the foot-and-mouth disease virus and the internal ribosome entry site (IRES) sequence from the crucifer-infecting tobamovirus to...

journal_title：Plant biotechnology journal

authors： Ha SH,Liang YS,Jung H,Ahn MJ,Suh SC,Kweon SJ,Kim DH,Kim YM,Kim JK

更新日期：2010-10-01 00:00:00

abstract：:Considered responsible for one million deaths in Ireland and widespread famine in the European continent during the 1840s, late blight, caused by Phytophthora infestans, remains the most devastating disease of potato (Solanum tuberosum L.) with about 15%-30% annual yield loss in sub-Saharan Africa, affecting mainly sm...

journal_title：Plant biotechnology journal

authors： Ghislain M,Byarugaba AA,Magembe E,Njoroge A,Rivera C,Román ML,Tovar JC,Gamboa S,Forbes GA,Kreuze JF,Barekye A,Kiggundu A

更新日期：2019-06-01 00:00:00

abstract：:Lignocellulosic feedstocks can be converted to biofuels, which can conceivably replace a large fraction of fossil fuels currently used for transformation. However, lignin, a prominent constituent of secondary cell walls, is an impediment to the conversion of cell walls to fuel: the recalcitrance problem. Biomass pretr...

journal_title：Plant biotechnology journal

authors： Poovaiah CR,Nageswara-Rao M,Soneji JR,Baxter HL,Stewart CN Jr

更新日期：2014-12-01 00:00:00

abstract：:The Chinese jujube (Ziziphus jujuba Mill.), a member of the Rhamnaceae family, is an important perennial fruit tree crop of substantial economic, ecological and nutritional value, and is also used as a traditional herbal medicine. Here, we report the resequencing of 493 jujube accessions, including 202 wild and 291 cu...

journal_title：Plant biotechnology journal

authors： Guo M,Zhang Z,Li S,Lian Q,Fu P,He Y,Qiao J,Xu K,Liu L,Wu M,Du Z,Li S,Wang J,Shao P,Yu Q,Xu G,Li D,Wang Y,Tian S,Zhao J,Feng X,Li R,Jiang W,Zhao X

更新日期：2020-09-18 00:00:00

abstract：:Developing new strategies for crop plants to respond to drought is crucial for their innovative breeding. The down-regulation of nuclear cap-binding proteins in Arabidopsis renders plants drought tolerant. The CBP80 gene in the potato cultivar Desiree was silenced using artificial microRNAs. Transgenic plants displaye...

journal_title：Plant biotechnology journal

authors： Pieczynski M,Marczewski W,Hennig J,Dolata J,Bielewicz D,Piontek P,Wyrzykowska A,Krusiewicz D,Strzelczyk-Zyta D,Konopka-Postupolska D,Krzeslowska M,Jarmolowski A,Szweykowska-Kulinska Z

更新日期：2013-05-01 00:00:00

abstract：:Nanobodies® (VHHs) provide powerful tools in therapeutic and biotechnological applications. Nevertheless, for some applications, bivalent antibodies perform much better, and for this, an Fc chain can be fused to the VHH domain, resulting in a bivalent homodimeric VHH-Fc complex. However, the production of bivalent ant...

journal_title：Plant biotechnology journal

authors： De Buck S,Nolf J,De Meyer T,Virdi V,De Wilde K,Van Lerberge E,Van Droogenbroeck B,Depicker A

更新日期：2013-10-01 00:00:00

abstract：:As an increasing number of genome sequences become available for a wide range of species, there is a growing understanding that the genome of a single individual is insufficient to represent the gene diversity within a whole species. Many studies examine the sequence diversity within genes, and this allelic variation ...

journal_title：Plant biotechnology journal

authors： Golicz AA,Batley J,Edwards D

更新日期：2016-04-01 00:00:00

abstract：:The capacity of the bread wheat (Triticum aestivum) genome to tolerate introgression from related genomes can be exploited for wheat improvement. A resistance to powdery mildew expressed by a derivative of the cross-bread wheat cv. Tähti × T. militinae (Tm) is known to be due to the incorporation of a Tm segment into ...

journal_title：Plant biotechnology journal

authors： Abrouk M,Balcárková B,Šimková H,Komínkova E,Martis MM,Jakobson I,Timofejeva L,Rey E,Vrána J,Kilian A,Järve K,Doležel J,Valárik M

更新日期：2017-02-01 00:00:00

abstract：:A precursor of miR156 (MsmiR156d) was cloned and overexpressed in alfalfa (Medicago sativa L.) as a means to enhance alfalfa biomass yield. Of the five predicted SPL genes encoded by the alfalfa genome, three (SPL6, SPL12 and SPL13) contain miR156 cleavage sites and their expression was down-regulated in transgenic al...

journal_title：Plant biotechnology journal

authors： Aung B,Gruber MY,Amyot L,Omari K,Bertrand A,Hannoufa A

更新日期：2015-08-01 00:00:00

abstract：:The production of biodegradable polymers that can be used to substitute petrochemical compounds in commercial products in transgenic plants is an important challenge for plant biotechnology. Nevertheless, it is often accompanied by reduced plant fitness. To decrease the phenotypic abnormalities of the sprout and to in...

journal_title：Plant biotechnology journal

authors： Hühns M,Neumann K,Hausmann T,Klemke F,Lockau W,Kahmann U,Kopertekh L,Staiger D,Pistorius EK,Reuther J,Waldvogel E,Wohlleben W,Effmert M,Junghans H,Neubauer K,Kragl U,Schmidt K,Schmidtke J,Broer I

更新日期：2009-12-01 00:00:00

abstract：:Genetically engineered (GE) rice lines expressing Lepidoptera-active insecticidal cry genes from the bacterium Bacillus thuringiensis (Bt) have been developed in China. Field surveys indicated that Bt rice harbours fewer rice planthoppers than non-Bt rice although planthoppers are not sensitive to the produced Bt Cry ...

journal_title：Plant biotechnology journal

authors： Wang X,Liu Q,Meissle M,Peng Y,Wu K,Romeis J,Li Y

更新日期：2018-10-01 00:00:00

abstract：:Low grain moisture at harvest is crucial for safe production, transport, and storage, but the genetic architecture of this trait in maize (Zea mays) remains elusive. Here, we measured the dynamic changes in grain moisture content in an association mapping panel of 513 diverse maize inbred lines at five successive stag...

journal_title：Plant biotechnology journal

authors： Li W,Yu Y,Wang L,Luo Y,Peng Y,Xu Y,Liu X,Wu S,Jian L,Xu J,Xiao Y,Yan J

更新日期：2021-01-01 00:00:00

abstract：:Transcription factors have been shown to control the activity of multiple stress response genes in a coordinated manner and therefore represent attractive targets for application in molecular plant breeding. We investigated the possibility of modulating the transcriptional regulation of drought and cold responses in t...

journal_title：Plant biotechnology journal

authors： Morran S,Eini O,Pyvovarenko T,Parent B,Singh R,Ismagul A,Eliby S,Shirley N,Langridge P,Lopato S

更新日期：2011-02-01 00:00:00