Using CRISPR/Cas9 genome editing in tomato to create a gibberellin-responsive dominant dwarf DELLA allele.

Abstract:

:The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid in the DELLA domain. This is the first report of a dominant dwarf PROCERA allele. This allele retains partial responsiveness to exogenously applied gibberellin. Heterozygotes show an intermediate phenotype at the seedling stage, but adult heterozygotes are as dwarfed as homozygotes.

journal_name

Plant Biotechnol J

authors

Tomlinson L,Yang Y,Emenecker R,Smoker M,Taylor J,Perkins S,Smith J,MacLean D,Olszewski NE,Jones JDG

doi

10.1111/pbi.12952

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

132-140

issue

1

eissn

1467-7644

issn

1467-7652

journal_volume

17

pub_type

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