Abstract:
:The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid in the DELLA domain. This is the first report of a dominant dwarf PROCERA allele. This allele retains partial responsiveness to exogenously applied gibberellin. Heterozygotes show an intermediate phenotype at the seedling stage, but adult heterozygotes are as dwarfed as homozygotes.
journal_name
Plant Biotechnol Jjournal_title
Plant biotechnology journalauthors
Tomlinson L,Yang Y,Emenecker R,Smoker M,Taylor J,Perkins S,Smith J,MacLean D,Olszewski NE,Jones JDGdoi
10.1111/pbi.12952subject
Has Abstractpub_date
2019-01-01 00:00:00pages
132-140issue
1eissn
1467-7644issn
1467-7652journal_volume
17pub_type
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