Application of two bicistronic systems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm.

abstract：:The plant pathogen, Pseudomonas syringae (Ps), together with related Ps species, infects and attacks a wide range of agronomically important crops, including tomato, kiwifruit, pepper, olive and soybean, causing economic losses. Currently, chemicals and introduced resistance genes are used to protect plants against th...

journal_title：Plant biotechnology journal

authors： Rooney WM,Grinter RW,Correia A,Parkhill J,Walker DC,Milner JJ

更新日期：2020-05-01 00:00:00

abstract：:Soya bean (Glycine max) and grass pea (Lathyrus sativus) seeds are important sources of dietary proteins; however, they also contain antinutritional metabolite oxalic acid (OA). Excess dietary intake of OA leads to nephrolithiasis due to the formation of calcium oxalate crystals in kidneys. Besides, OA is also a known...

journal_title：Plant biotechnology journal

authors： Kumar V,Chattopadhyay A,Ghosh S,Irfan M,Chakraborty N,Chakraborty S,Datta A

更新日期：2016-06-01 00:00:00

abstract：:S-adenosyl-L-methionine (SAM) synthetase is the key enzyme involved in the biosynthesis of SAM, which serves as a common precursor for polyamines (PAs) and ethylene. A SAM synthetase cDNA (SlSAMS1) was introduced into the tomato genome using the Agrobacterium tumefaciens transformation method. Transgenic plants overex...

journal_title：Plant biotechnology journal

authors： Gong B,Li X,VandenLangenberg KM,Wen D,Sun S,Wei M,Li Y,Yang F,Shi Q,Wang X

更新日期：2014-08-01 00:00:00

abstract：:Food supplementation with the conditionally essential amino acid arginine (Arg) has been shown to have nutritional benefits. Degradation of cyanophycin (CGP), a peptide polymer used for nitrogen storage by cyanobacteria, requires cyanophycinase (CGPase) and results in the release of β-aspartic acid (Asp)-Arg dipeptide...

journal_title：Plant biotechnology journal

authors： Ponndorf D,Ehmke S,Walliser B,Thoss K,Unger C,Görs S,Daş G,Metges CC,Broer I,Nausch H

更新日期：2017-05-01 00:00:00

abstract：:It is widely recognized that biofuel production from lignocellulosic materials is limited by inadequate technology to efficiently and economically release fermentable sugars from the complex multi-polymeric raw materials. Therefore, endoglucanases, exoglucanase, pectate lyases, cutinase, swollenin, xylanase, acetyl xy...

journal_title：Plant biotechnology journal

authors： Verma D,Kanagaraj A,Jin S,Singh ND,Kolattukudy PE,Daniell H

更新日期：2010-04-01 00:00:00

abstract：:Genome modification by homology-directed repair (HDR) is an attractive tool for the controlled genetic manipulation of plants. Here, we report the HDR-mediated gene exchange of expression cassettes in tobacco BY-2 cells using a designed zinc finger nuclease (ZFN). The target contained a 7-kb fragment flanked by two ZF...

journal_title：Plant biotechnology journal

authors： Schneider K,Schiermeyer A,Dolls A,Koch N,Herwartz D,Kirchhoff J,Fischer R,Russell SM,Cao Z,Corbin DR,Sastry-Dent L,Ainley WM,Webb SR,Schinkel H,Schillberg S

更新日期：2016-04-01 00:00:00

abstract：:Which factors limit metabolite accumulation in plant cells? Are theories on flux control effective at explaining the results? Many biotechnologists cling to the idea that every pathway has a rate limiting enzyme and target such enzymes first in order to modulate fluxes. This often translates into large effects on meta...

journal_title：Plant biotechnology journal

authors： Morandini P

更新日期：2013-02-01 00:00:00

abstract：:Phytochrome-interacting factors (PIFs) play major roles in regulating plant growth and development, but their roles in drought stress remain elusive. Here, we cloned and characterized a maize (Zea mays) PIF transcription factor, ZmPIF1. The expression level of ZmPIF1 was significantly induced by independent drought an...

journal_title：Plant biotechnology journal

authors： Gao Y,Wu M,Zhang M,Jiang W,Ren X,Liang E,Zhang D,Zhang C,Xiao N,Li Y,Dai Y,Chen J

更新日期：2018-07-01 00:00:00

abstract：:Production of novel transgenic floricultural crops with altered petal properties requires transgenes that confer a useful trait and petal-specific promoters. Several promoters have been shown to control transgenes in petals. However, all suffer from inherent drawbacks such as low petal specificity and restricted activ...

journal_title：Plant biotechnology journal

authors： Azuma M,Morimoto R,Hirose M,Morita Y,Hoshino A,Iida S,Oshima Y,Mitsuda N,Ohme-Takagi M,Shiratake K

更新日期：2016-01-01 00:00:00

abstract：:With the aim of increasing the methionine level in alfalfa (Medicago sativa L.) and thus improving its nutritional quality, we produced transgenic alfalfa plants that expressed the Arabidopsis cystathionine gamma-synthase (AtCGS), the enzyme that controls the synthesis of the first intermediate metabolite in the methi...

journal_title：Plant biotechnology journal

authors： Avraham T,Badani H,Galili S,Amir R

更新日期：2005-01-01 00:00:00

abstract：:Approximately 20,000 of the rice-FOX Arabidopsis transgenic lines, which overexpress 13,000 rice full-length cDNAs at random in Arabidopsis, were screened for bacterial disease resistance by dip inoculation with Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). The identities of the overexpressed genes were determi...

journal_title：Plant biotechnology journal

authors： Dubouzet JG,Maeda S,Sugano S,Ohtake M,Hayashi N,Ichikawa T,Kondou Y,Kuroda H,Horii Y,Matsui M,Oda K,Hirochika H,Takatsuji H,Mori M

更新日期：2011-05-01 00:00:00

abstract：:There is a pressing need to develop novel antibacterial agents given the widespread antibiotic resistance among pathogenic bacteria and the low specificity of the drugs available. Endolysins are antibacterial proteins that are produced by bacteriophage-infected cells to digest the bacterial cell wall for phage progeny...

journal_title：Plant biotechnology journal

authors： Stoffels L,Taunt HN,Charalambous B,Purton S

更新日期：2017-09-01 00:00:00

abstract：:Increased use of nitrogen fertilizers has deleterious impact on the environment. Increase in yield potential at low nitrogen supply is regarded as a cereal breeding goal for future agricultural sustainability. Although natural variations of nitrogen transporters have been investigated, key genes associated with assimi...

journal_title：Plant biotechnology journal

authors： Yu J,Xuan W,Tian Y,Fan L,Sun J,Tang W,Chen G,Wang B,Liu Y,Wu W,Liu X,Jiang X,Zhou C,Dai Z,Xu D,Wang C,Wan J

更新日期：2021-01-01 00:00:00

abstract：:Sugarcane (Saccharum spp.) is a highly energy-efficient crop primarily for sugar and bio-ethanol production. Sugarcane genetics and cultivar improvement have been extremely challenging largely due to its complex genomes with high polyploidy levels. In this study, we deeply sequenced the coding regions of 307 sugarcane...

journal_title：Plant biotechnology journal

authors： Yang X,Song J,Todd J,Peng Z,Paudel D,Luo Z,Ma X,You Q,Hanson E,Zhao Z,Zhao Y,Zhang J,Ming R,Wang J

更新日期：2019-02-01 00:00:00

abstract：:Glycolate is produced in autotrophic cells under high temperatures and Ci -limitation via oxygenation of ribulose-1,5-bisphosphate. In unicellular algae, glycolate is lost via excretion or metabolized via the C2 cycle by consuming reductants, ATP and CO2 emission (photorespiration). Therefore, photorespiration is an i...

journal_title：Plant biotechnology journal

authors： Taubert A,Jakob T,Wilhelm C

更新日期：2019-08-01 00:00:00

abstract：:Whole-genome sequencing-based bulked segregant analysis (BSA) for mapping quantitative trait loci (QTL) provides an efficient alternative approach to conventional QTL analysis as it significantly reduces the scale and cost of analysis with comparable power to QTL detection using full mapping population. We tested the ...

journal_title：Plant biotechnology journal

authors： Deokar A,Sagi M,Daba K,Tar'an B

更新日期：2019-01-01 00:00:00

abstract：:Clustered regularly interspaced short palindromic repeat (CRISPR) and Cas9-associated protein systems provide a powerful genetic manipulation tool that can drive plant research forward. Nuclease-dead Cas9 (dCas9) is an enzymatically inactive mutant of Cas9 in which its endonuclease activity is non-functional. The appl...

journal_title：Plant biotechnology journal

authors： Moradpour M,Abdulah SNA

更新日期：2020-01-01 00:00:00

abstract：:Although it is one of the major crops in the world, corn has poor nutritional quality for human and animal consumption due to its low lysine content. Here, we report a method of simultaneous expression of a deregulated lysine biosynthetic enzyme, CordapA, and reduction of a bifunctional lysine degradation enzyme, lysi...

journal_title：Plant biotechnology journal

authors： Frizzi A,Huang S,Gilbertson LA,Armstrong TA,Luethy MH,Malvar TM

更新日期：2008-01-01 00:00:00

abstract：:The plant immune system involves detection of pathogens via both cell-surface and intracellular receptors. Both receptor classes can induce transcriptional reprogramming that elevates disease resistance. To assess differential gene expression during plant immunity, we developed and deployed quantitative sequence captu...

journal_title：Plant biotechnology journal

authors： Ding P,Ngou BPM,Furzer OJ,Sakai T,Shrestha RK,MacLean D,Jones JDG

更新日期：2020-07-01 00:00:00

abstract：:Steviol glycosides (SGs) are extracted from Stevia leaves for use as a natural sweetener. Among SGs, stevioside is most abundant in leaf extracts followed by rebaudioside A (Reb A). However, Reb A is of particular interest because of its sweeter and more pleasant taste compared to stevioside. Therefore, the developmen...

journal_title：Plant biotechnology journal

authors： Kim MJ,Zheng J,Liao MH,Jang IC

更新日期：2019-06-01 00:00:00

abstract：:Globally, wheat is the most widely grown crop and one of the three most important crops for human and livestock feed. However, the complex nature of the wheat genome has, until recently, resulted in a lack of single nucleotide polymorphism (SNP)-based molecular markers of practical use to wheat breeders. Recently, lar...

journal_title：Plant biotechnology journal

authors： Allen AM,Barker GL,Wilkinson P,Burridge A,Winfield M,Coghill J,Uauy C,Griffiths S,Jack P,Berry S,Werner P,Melichar JP,McDougall J,Gwilliam R,Robinson P,Edwards KJ

更新日期：2013-04-01 00:00:00

abstract：:We describe the site-directed integration (SDI) system for Agrobacterium-mediated transformation to precisely integrate a single copy of a desired gene into a predefined target locus by recombinase-mediated cassette exchange (RMCE). The system requires the selection of a transformed line with an integrated copy of a t...

journal_title：Plant biotechnology journal

authors： Nanto K,Yamada-Watanabe K,Ebinuma H

更新日期：2005-03-01 00:00:00

abstract：:Although conifers are of immense ecological and economic value, bioengineering of their chloroplasts remains undeveloped. Understanding the chloroplast genomic organization of conifers can facilitate their bioengineering. Members of the conifer II clade (or cupressophytes) are highly diverse in both morphologic featur...

journal_title：Plant biotechnology journal

authors： Wu CS,Chaw SM

更新日期：2014-04-01 00:00:00

abstract：:The 1980s and 1990s saw a major expansion of biotechnology into new areas of science including genomics and recombinant technologies. This was coupled to the widespread emergence of academics into the commercial sector as they were encouraged to spin out companies or commercialize their intellectual property. There we...

journal_title：Plant biotechnology journal

authors： Paul MJ,Thangaraj H,Ma JK

更新日期：2015-10-01 00:00:00

abstract：:The advent of genetically modified crops in the late 1980s triggered a regulatory response to the relatively new field of plant genetic engineering. Over a 7-year period, a new regulatory framework was created, based on scientific principles that focused on risk mitigation. The process was transparent and deliberately...

journal_title：Plant biotechnology journal

authors： Smyth S,McHughen A

更新日期：2008-04-01 00:00:00

abstract：:Grain size and plant architecture are critical factors determining crop productivity. Here, we performed gene editing of the MIR396 gene family in rice and found that MIR396e and MIR396f are two important regulators of grain size and plant architecture. mir396ef mutations can increase grain yield by increasing grain s...

journal_title：Plant biotechnology journal

authors： Miao C,Wang D,He R,Liu S,Zhu JK

更新日期：2020-02-01 00:00:00

abstract：:A hybrid-proline-rich protein encoding gene (CcHyPRP) has been isolated and characterized, for the first time, from the subtracted cDNA library of pigeonpea (Cajanus cajan L.) plants subjected to drought stress. Functionality of CcHyPRP has been validated for abiotic stress tolerance using the heterologous yeast and A...

journal_title：Plant biotechnology journal

authors： Priyanka B,Sekhar K,Reddy VD,Rao KV

更新日期：2010-01-01 00:00:00

abstract：:Porcine circovirus type 2 (PCV-2) is the main causative agent associated with a group of diseases collectively known as porcine circovirus-associated disease (PCAD). There is a significant economic strain on the global swine industry due to PCAD and the production of commercial PCV-2 vaccines is expensive. Plant expre...

journal_title：Plant biotechnology journal

authors： Gunter CJ,Regnard GL,Rybicki EP,Hitzeroth II

更新日期：2019-09-01 00:00:00

abstract：:Aphids produce gel saliva during feeding which forms a sheath around the stylet as it penetrates through the apoplast. The sheath is required for the sustained ingestion of phloem sap from sieve elements and is thought to form when the structural sheath protein (SHP) is cross-linked by intermolecular disulphide bridge...

journal_title：Plant biotechnology journal

authors： Abdellatef E,Will T,Koch A,Imani J,Vilcinskas A,Kogel KH

更新日期：2015-08-01 00:00:00

abstract：:Plant chloroplasts are promising vehicles for recombinant protein production, but the process of protein folding in these organelles is not well understood in comparison with that in prokaryotic systems, such as Escherichia coli. This is particularly true for disulphide bond formation which is crucial for the biologic...

journal_title：Plant biotechnology journal

authors： Bally J,Paget E,Droux M,Job C,Job D,Dubald M

更新日期：2008-01-01 00:00:00