Abstract:
:Dioxin residues widely contaminate soil and agricultural products at low concentrations and may accumulate in organisms at the top of food chains owing to their physicochemical properties. In this study, we have developed novel, dioxin-inducible, reporter gene expression systems regulated by recombinant aryl hydrocarbon receptors (AhRs). The recombinant AhRs, referred to as XDVs, consist of the DNA-binding domain of the bacterial repressor protein LexA, a 90-kDa heat shock protein- and ligand-binding regulatory domain from mouse AhR, and the transactivation domain of herpes simplex virus regulatory protein VP16. Transgenic tobacco plants carrying XDVs absorb various AhR ligands, including 3-methylcholanthrene, beta-naphthoflavone and indigo from solid medium and vermiculite, and show dose- and time-dependent expression of the beta-glucuronidase reporter gene. The results clearly suggest that XDVs are functional transcription factors that respond to AhR ligands, and that the XDV-mediated reporter gene expression system is applicable to bioassays for dioxin residues in the environment.
journal_name
Plant Biotechnol Jjournal_title
Plant biotechnology journalauthors
Kodama S,Okada K,Akimoto K,Inui H,Ohkawa Hdoi
10.1111/j.1467-7652.2008.00378.xsubject
Has Abstractpub_date
2009-02-01 00:00:00pages
119-28issue
2eissn
1467-7644issn
1467-7652pii
PBI378journal_volume
7pub_type
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