Abstract:
:Dengue fever is a mosquito (Aedes aegypti) -transmitted viral disease that is endemic in more than 125 countries around the world. There are four serotypes of the dengue virus (DENV 1-4) and a safe and effective dengue vaccine must provide protection against all four serotypes. To date, the first vaccine, Dengvaxia (CYD-TDV), is available after many decades' efforts, but only has moderate efficacy. More effective and affordable vaccines are hence required. Plants offer promising vaccine production platforms and food crops offer additional advantages for the production of edible human and animal vaccines, thus eliminating the need for expensive fermentation, purification, cold storage and sterile delivery. Oral vaccines can elicit humoural and cellular immunity via both the mucosal and humoral immune systems. Here, we report the production of tetravalent EDIII antigen (EDIII-1-4) in stably transformed lettuce chloroplasts. Transplastomic EDIII-1-4-expressing lettuce lines were obtained and homoplasmy was verified by Southern blot analysis. Expression of EDIII-1-4 antigens was demonstrated by immunoblotting, with the EDIII-1-4 antigen accumulating to 3.45% of the total protein content. Immunological assays in rabbits showed immunogenicity of EDIII-1-4. Our in vitro gastrointestinal digestion analysis revealed that EDIII-1-4 antigens are well protected when passing through the oral and gastric digestion phases but underwent degradation during the intestinal phase. Our results demonstrate that lettuce chloroplast engineering is a promising approach for future production of an affordable oral dengue vaccine.
journal_name
Plant Biotechnol Jjournal_title
Plant biotechnology journalauthors
van Eerde A,Gottschamel J,Bock R,Hansen KEA,Munang'andu HM,Daniell H,Liu Clarke Jdoi
10.1111/pbi.13065subject
Has Abstractpub_date
2019-07-01 00:00:00pages
1408-1417issue
7eissn
1467-7644issn
1467-7652journal_volume
17pub_type
杂志文章abstract::Broad spectrum protection against different insects and pathogens requires multigene engineering. However, such broad spectrum protection against biotic stress is provided by a single protein in some medicinal plants. Therefore, tobacco chloroplasts were transformed with the agglutinin gene from Pinellia ternata (pta)...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2011.00663.x
更新日期:2012-04-01 00:00:00
abstract::Sumoylation is a posttranslational regulatory process in higher eukaryotes modifying substrate proteins through conjugation of small ubiquitin-related modifiers (SUMOs). Sumoylation modulates protein stability, subcellular localization and activity; thus, it regulates most cellular functions including response to envi...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12030
更新日期:2013-05-01 00:00:00
abstract::Although conifers are of immense ecological and economic value, bioengineering of their chloroplasts remains undeveloped. Understanding the chloroplast genomic organization of conifers can facilitate their bioengineering. Members of the conifer II clade (or cupressophytes) are highly diverse in both morphologic featur...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12141
更新日期:2014-04-01 00:00:00
abstract::Reverse transcription-polymerase chain reaction (RT-PCR) approaches have been used in a large proportion of transcriptome analyses published to date. The accuracy of the results obtained by this method strongly depends on accurate transcript normalization using stably expressed genes, known as references. Statistical ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2008.00346.x
更新日期:2008-08-01 00:00:00
abstract::Whole-genome sequencing-based bulked segregant analysis (BSA) for mapping quantitative trait loci (QTL) provides an efficient alternative approach to conventional QTL analysis as it significantly reduces the scale and cost of analysis with comparable power to QTL detection using full mapping population. We tested the ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12964
更新日期:2019-01-01 00:00:00
abstract::Starch is an important renewable raw material with an increasing number of applications. Several attempts have been made to obtain plants that produce modified versions of starch or higher starch yield. Most of the approaches designed to increase the levels of starch have focused on the increment of the amount of ADP-...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2011.00626.x
更新日期:2011-12-01 00:00:00
abstract::In the past few years, the usefulness of transient expression assays has continuously increased for the characterization of unknown gene function and metabolic pathways. In grapevine (Vitis vinifera L.), one of the most economically important fruit crops in the world, recent systematic sequencing projects produced man...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/pbi.12294
更新日期:2014-12-01 00:00:00
abstract::Phytochrome-interacting factors (PIFs) play major roles in regulating plant growth and development, but their roles in drought stress remain elusive. Here, we cloned and characterized a maize (Zea mays) PIF transcription factor, ZmPIF1. The expression level of ZmPIF1 was significantly induced by independent drought an...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12878
更新日期:2018-07-01 00:00:00
abstract::Downy mildew (Plasmopara halstedii (Farl.) Berlese et de Toni) is a serious foliar pathogen of cultivated sunflower (Helianthus annuus L.). Genetic resistance is conditioned by several linked downy mildew resistance gene specificities in the HaRGC1 cluster of TIR-NBS-LRR resistance gene candidates (RGCs) on linkage gr...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1046/j.1467-7652.2003.00016.x
更新日期:2003-05-01 00:00:00
abstract::Drought conditions are among the most serious challenges to crop production worldwide. Here, we report the results of field evaluations of transgenic rice plants overexpressing OsNAC5, under the control of either the root-specific (RCc3) or constitutive (GOS2) promoters. Field evaluations over three growing seasons re...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12011
更新日期:2013-01-01 00:00:00
abstract::Transgenic rice expressing soybean glycinin in its endosperm was crossed with two types of low-glutelin mutants to determine how much storage the protein mutants can contribute to increases in glycinin accumulation. The glycinin level (102 microg/100 mg seed) in the parental transgenic line was enhanced to approximate...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1046/j.1467-7652.2003.00038.x
更新日期:2003-11-01 00:00:00
abstract::Flowerless trait is highly desirable for poplar because it can prevent pollen- and seed-mediated transgene flow. We have isolated the second intron of PTAG2, an AGAMOUS (AG) orthologue from Populus trichocarpa. By fusing this intron sequence to a minimal 35S promoter sequence, we created two artificial promoters, fPTA...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12581
更新日期:2016-12-01 00:00:00
abstract::MicroRNAs (miRNAs) are a class of regulatory small RNAs (sRNAs) that down-regulate target genes through mRNA cleavage or translational inhibition. miRNA is known to play an important role in the root development and environmental responses in both the Arabidopsis and rice. However, little information is available to f...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12218
更新日期:2014-10-01 00:00:00
abstract::Tef, Eragrostis tef (Zucc.) Trotter, is the most important cereal in Ethiopia. Tef is cultivated by more than five million small-scale farmers annually and constitutes the staple food for more than half of the population of 80 million. The crop is preferred by both farmers and consumers due to its beneficial traits as...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/pbi.12199
更新日期:2014-06-01 00:00:00
abstract::Burley tobaccos (Nicotiana tabacum) display a nitrogen-use-deficiency phenotype that is associated with the accumulation of high levels of nitrate within the leaf, a trait correlated with production of a class of compounds referred to as tobacco-specific nitrosamines (TSNAs). Two TSNA species, 4-(methylnitrosamino)-1-...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12510
更新日期:2016-07-01 00:00:00
abstract::Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer c...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12359
更新日期:2016-01-01 00:00:00
abstract::China is the origin and evolutionary centre of Oriental pears. Pyrus betuleafolia is a wild species native to China and distributed in the northern region, and it is widely used as rootstock. Here, we report the de novo assembly of the genome of P. betuleafolia-Shanxi Duli using an integrated strategy that combines Pa...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13226
更新日期:2020-02-01 00:00:00
abstract::Drought conditions limit agricultural production by preventing crops from reaching their genetically predetermined maximum yields. Here, we present the results of field evaluations of rice overexpressing OsNAC9, a member of the rice NAC domain family. Root-specific (RCc3) and constitutive (GOS2) promoters were used to...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2012.00697.x
更新日期:2012-09-01 00:00:00
abstract::Citrus fruit has a unique structure with soft leathery peel and pulp containing vascular bundles and several segments with many juice sacs. The function and morphology of each fruit tissue are different. Therefore, analysis at the organ-wide or mixed-tissue level inevitably obscures many tissue-specific phenomena. Hig...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13549
更新日期:2021-01-20 00:00:00
abstract::Traditional breeding methods are hindered in bananas due to the fact that major cultivars are sterile, parthenocarpic, triploid and thus clonally propagated. This has resulted in a narrow genetic base and limited resilience to biotic and abiotic stresses. Mutagenesis of in vitro propagated bananas is one method to int...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12901
更新日期:2018-02-24 00:00:00
abstract::S-adenosyl-L-methionine (SAM) synthetase is the key enzyme involved in the biosynthesis of SAM, which serves as a common precursor for polyamines (PAs) and ethylene. A SAM synthetase cDNA (SlSAMS1) was introduced into the tomato genome using the Agrobacterium tumefaciens transformation method. Transgenic plants overex...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12173
更新日期:2014-08-01 00:00:00
abstract::Lignocellulosic feedstocks can be converted to biofuels, which can conceivably replace a large fraction of fossil fuels currently used for transformation. However, lignin, a prominent constituent of secondary cell walls, is an impediment to the conversion of cell walls to fuel: the recalcitrance problem. Biomass pretr...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/pbi.12225
更新日期:2014-12-01 00:00:00
abstract::Threonine (Thr) is one of a few limiting essential amino acids (EAAs) in the animal feed industry, and its level in feed rations can impact production of important meat sources, such as swine and poultry. Threonine as well as EAAs lysine (Lys) and methionine (Met) are all synthesized via the aspartate family pathway. ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2010.00545.x
更新日期:2011-02-01 00:00:00
abstract::Squalene is a linear intermediate to nearly all classes of triterpenes and sterols and is itself highly valued for its use in wide range of industrial applications. Another unique linear triterpene is botryococcene and its methylated derivatives generated by the alga Botryococcus braunii race B, which are progenitors ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12855
更新日期:2018-06-01 00:00:00
abstract::Receptor-like kinases (RLKs) are important components of plant innate immunity. Although recent studies have revealed that the RLK suppressor of BIR1-1 (SOBIR1) can interact with multiple receptor-like proteins and is required for resistance against fungal pathogens, how the signal is transduced and triggers immune re...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12954
更新日期:2019-01-01 00:00:00
abstract::Genetic improvement of fibre quality is one of the main breeding goals for the upland cotton, Gossypium hirsutum, but there are difficulties with precise selection of traits. Therefore, it is important to improve the understanding of the genetic basis of phenotypic variation. In this study, we conducted phenotyping an...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12693
更新日期:2017-08-01 00:00:00
abstract::Sugarcane (Saccharum spp.) is a highly energy-efficient crop primarily for sugar and bio-ethanol production. Sugarcane genetics and cultivar improvement have been extremely challenging largely due to its complex genomes with high polyploidy levels. In this study, we deeply sequenced the coding regions of 307 sugarcane...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12992
更新日期:2019-02-01 00:00:00
abstract::The intensification of agriculture and the development of synthetic insecticides enabled worldwide grain production to more than double in the last third of the 20th century. However, the heavy dependence and, in some cases, overuse of insecticides has been responsible for negative environmental and ecological impacts...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/pbi.12363
更新日期:2015-06-01 00:00:00
abstract::Differences in gene sequences, many of which are single nucleotide polymorphisms, underlie some of the most important traits in plants. With humanity facing significant challenges to increase global agricultural productivity, there is an urgent need to accelerate the development of these traits in plants. oligonucleot...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/pbi.12496
更新日期:2016-02-01 00:00:00
abstract::Glyphosate is a popular, systemic, broad-spectrum herbicide used in modern agriculture. Being a structural analog of phosphoenolpyruvate (PEP), it inhibits 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) which is responsible for the biosynthesis of aromatic amino acids and various aromatic secondary metabolites. T...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13428
更新日期:2020-12-01 00:00:00