Oligonucleotide-directed mutagenesis for precision gene editing.

Abstract:

:Differences in gene sequences, many of which are single nucleotide polymorphisms, underlie some of the most important traits in plants. With humanity facing significant challenges to increase global agricultural productivity, there is an urgent need to accelerate the development of these traits in plants. oligonucleotide-directed mutagenesis (ODM), one of the many tools of Cibus' Rapid Trait Development System (RTDS(™) ) technology, offers a rapid, precise and non-transgenic breeding alternative for trait improvement in agriculture to address this urgent need. This review explores the application of ODM as a precision genome editing technology, with emphasis on using oligonucleotides to make targeted edits in plasmid, episomal and chromosomal DNA of bacterial, fungal, mammalian and plant systems. The process of employing ODM by way of RTDS technology has been improved in many ways by utilizing a fluorescence conversion system wherein a blue fluorescent protein (BFP) can be changed to a green fluorescent protein (GFP) by editing a single nucleotide of the BFP gene (CAC→TAC; H66 to Y66). For example, dependent on oligonucleotide length, applying oligonucleotide-mediated technology to target the BFP transgene in Arabidopsis thaliana protoplasts resulted in up to 0.05% precisely edited GFP loci. Here, the development of traits in commercially relevant plant varieties to improve crop performance by genome editing technologies such as ODM, and by extension RTDS, is reviewed.

journal_name

Plant Biotechnol J

authors

Sauer NJ,Mozoruk J,Miller RB,Warburg ZJ,Walker KA,Beetham PR,Schöpke CR,Gocal GF

doi

10.1111/pbi.12496

subject

Has Abstract

pub_date

2016-02-01 00:00:00

pages

496-502

issue

2

eissn

1467-7644

issn

1467-7652

journal_volume

14

pub_type

杂志文章,评审
  • CRISPR/Cas9-mediated knockout of Ms1 enables the rapid generation of male-sterile hexaploid wheat lines for use in hybrid seed production.

    abstract::The development and adoption of hybrid seed technology have led to dramatic increases in agricultural productivity. However, it has been a challenge to develop a commercially viable platform for the production of hybrid wheat (Triticum aestivum) seed due to wheat's strong inbreeding habit. Recently, a novel platform f...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13106

    authors: Okada A,Arndell T,Borisjuk N,Sharma N,Watson-Haigh NS,Tucker EJ,Baumann U,Langridge P,Whitford R

    更新日期:2019-10-01 00:00:00

  • Pinellia ternata agglutinin expression in chloroplasts confers broad spectrum resistance against aphid, whitefly, Lepidopteran insects, bacterial and viral pathogens.

    abstract::Broad spectrum protection against different insects and pathogens requires multigene engineering. However, such broad spectrum protection against biotic stress is provided by a single protein in some medicinal plants. Therefore, tobacco chloroplasts were transformed with the agglutinin gene from Pinellia ternata (pta)...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2011.00663.x

    authors: Jin S,Zhang X,Daniell H

    更新日期:2012-04-01 00:00:00

  • Silencing the expression of the salivary sheath protein causes transgenerational feeding suppression in the aphid Sitobion avenae.

    abstract::Aphids produce gel saliva during feeding which forms a sheath around the stylet as it penetrates through the apoplast. The sheath is required for the sustained ingestion of phloem sap from sieve elements and is thought to form when the structural sheath protein (SHP) is cross-linked by intermolecular disulphide bridge...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12322

    authors: Abdellatef E,Will T,Koch A,Imani J,Vilcinskas A,Kogel KH

    更新日期:2015-08-01 00:00:00

  • Light-dependent regulation of ascorbate in tomato by a monodehydroascorbate reductase localized in peroxisomes and the cytosol.

    abstract::Ascorbate is a powerful antioxidant in plants, and its levels are an important quality criteria in commercial species. Factors influencing these levels include environmental variations, particularly light, and the genetic control of its biosynthesis, recycling and degradation. One of the genes involved in the recyclin...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12020

    authors: Gest N,Garchery C,Gautier H,Jiménez A,Stevens R

    更新日期:2013-04-01 00:00:00

  • CRISPR/Cas9-mediated knockout of six glycosyltransferase genes in Nicotiana benthamiana for the production of recombinant proteins lacking β-1,2-xylose and core α-1,3-fucose.

    abstract::Plants offer fast, flexible and easily scalable alternative platforms for the production of pharmaceutical proteins, but differences between plant and mammalian N-linked glycans, including the presence of β-1,2-xylose and core α-1,3-fucose residues in plants, can affect the activity, potency and immunogenicity of plan...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12981

    authors: Jansing J,Sack M,Augustine SM,Fischer R,Bortesi L

    更新日期:2019-02-01 00:00:00

  • Activity-based proteomics reveals nine target proteases for the recombinant protein-stabilizing inhibitor SlCYS8 in Nicotiana benthamiana.

    abstract::Co-expression of protease inhibitors like the tomato cystatin SlCYS8 is useful to increase recombinant protein production in plants, but key proteases involved in protein proteolysis are still unknown. Here, we performed activity-based protein profiling to identify proteases that are inhibited by SlCYS8 in agroinfiltr...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13092

    authors: Jutras PV,Grosse-Holz F,Kaschani F,Kaiser M,Michaud D,van der Hoorn RAL

    更新日期:2019-08-01 00:00:00

  • The TaDREB3 transgene transferred by conventional crossings to different genetic backgrounds of bread wheat improves drought tolerance.

    abstract::Drought tolerance of the wheat cultivar Bobwhite was previously enhanced by transformation with a construct containing the wheat DREB3 gene driven by the stress-inducible maize Rab17 promoter. Progeny of a single T2 transgenic line were used as pollinators in crosses with four elite bread wheat cultivars from Western ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12385

    authors: Shavrukov Y,Baho M,Lopato S,Langridge P

    更新日期:2016-01-01 00:00:00

  • Engineering starch accumulation by manipulation of phosphate metabolism of starch.

    abstract::A new understanding of leaf starch degradation has emerged in the last 10 years. It has been shown that starch phosphorylation and dephosphorylation are critical components of this process. Glucan, water dikinase (GWD) (and phosphoglucan, water dikinase) adds phosphate to starch, and phosphoglucan phosphatase (SEX4) r...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2012.00684.x

    authors: Weise SE,Aung K,Jarou ZJ,Mehrshahi P,Li Z,Hardy AC,Carr DJ,Sharkey TD

    更新日期:2012-06-01 00:00:00

  • Engineering bacteriocin-mediated resistance against the plant pathogen Pseudomonas syringae.

    abstract::The plant pathogen, Pseudomonas syringae (Ps), together with related Ps species, infects and attacks a wide range of agronomically important crops, including tomato, kiwifruit, pepper, olive and soybean, causing economic losses. Currently, chemicals and introduced resistance genes are used to protect plants against th...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13294

    authors: Rooney WM,Grinter RW,Correia A,Parkhill J,Walker DC,Milner JJ

    更新日期:2020-05-01 00:00:00

  • Generation of marker-free transgenic hexaploid wheat via an Agrobacterium-mediated co-transformation strategy in commercial Chinese wheat varieties.

    abstract::Genotype specificity is a big problem lagging the development of efficient hexaploid wheat transformation system. Increasingly, the biosecurity of genetically modified organisms is garnering public attention, so the generation of marker-free transgenic plants is very important to the eventual potential commercial rele...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12660

    authors: Wang K,Liu H,Du L,Ye X

    更新日期:2017-05-01 00:00:00

  • Characterization of scientific studies usually cited as evidence of adverse effects of GM food/feed.

    abstract::GM crops are the most studied crops in history. Approximately 5% of the safety studies on them show adverse effects that are a cause for concern and tend to be featured in media reports. Although these reports are based on just a handful of GM events, they are used to cast doubt on all GM crops. Furthermore, they tend...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/pbi.12798

    authors: Sánchez MA,Parrott WA

    更新日期:2017-10-01 00:00:00

  • Foreign gene products can be enhanced by introduction into low storage protein mutants.

    abstract::Transgenic rice expressing soybean glycinin in its endosperm was crossed with two types of low-glutelin mutants to determine how much storage the protein mutants can contribute to increases in glycinin accumulation. The glycinin level (102 microg/100 mg seed) in the parental transgenic line was enhanced to approximate...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1046/j.1467-7652.2003.00038.x

    authors: Tada Y,Utsumi S,Takaiwa F

    更新日期:2003-11-01 00:00:00

  • Stable production of cyanophycinase in Nicotiana benthamiana and its functionality to hydrolyse cyanophycin in the murine intestine.

    abstract::Food supplementation with the conditionally essential amino acid arginine (Arg) has been shown to have nutritional benefits. Degradation of cyanophycin (CGP), a peptide polymer used for nitrogen storage by cyanobacteria, requires cyanophycinase (CGPase) and results in the release of β-aspartic acid (Asp)-Arg dipeptide...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12658

    authors: Ponndorf D,Ehmke S,Walliser B,Thoss K,Unger C,Görs S,Daş G,Metges CC,Broer I,Nausch H

    更新日期:2017-05-01 00:00:00

  • Enhanced OsNLP4-OsNiR cascade confers nitrogen use efficiency by promoting tiller number in rice.

    abstract::Increased use of nitrogen fertilizers has deleterious impact on the environment. Increase in yield potential at low nitrogen supply is regarded as a cereal breeding goal for future agricultural sustainability. Although natural variations of nitrogen transporters have been investigated, key genes associated with assimi...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13450

    authors: Yu J,Xuan W,Tian Y,Fan L,Sun J,Tang W,Chen G,Wang B,Liu Y,Wu W,Liu X,Jiang X,Zhou C,Dai Z,Xu D,Wang C,Wan J

    更新日期:2021-01-01 00:00:00

  • Both the stroma and thylakoid lumen of tobacco chloroplasts are competent for the formation of disulphide bonds in recombinant proteins.

    abstract::Plant chloroplasts are promising vehicles for recombinant protein production, but the process of protein folding in these organelles is not well understood in comparison with that in prokaryotic systems, such as Escherichia coli. This is particularly true for disulphide bond formation which is crucial for the biologic...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2007.00298.x

    authors: Bally J,Paget E,Droux M,Job C,Job D,Dubald M

    更新日期:2008-01-01 00:00:00

  • Genome-wide association study discovered genetic variation and candidate genes of fibre quality traits in Gossypium hirsutum L.

    abstract::Genetic improvement of fibre quality is one of the main breeding goals for the upland cotton, Gossypium hirsutum, but there are difficulties with precise selection of traits. Therefore, it is important to improve the understanding of the genetic basis of phenotypic variation. In this study, we conducted phenotyping an...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12693

    authors: Sun Z,Wang X,Liu Z,Gu Q,Zhang Y,Li Z,Ke H,Yang J,Wu J,Wu L,Zhang G,Zhang C,Ma Z

    更新日期:2017-08-01 00:00:00

  • Highly rearranged and size-variable chloroplast genomes in conifers II clade (cupressophytes): evolution towards shorter intergenic spacers.

    abstract::Although conifers are of immense ecological and economic value, bioengineering of their chloroplasts remains undeveloped. Understanding the chloroplast genomic organization of conifers can facilitate their bioengineering. Members of the conifer II clade (or cupressophytes) are highly diverse in both morphologic featur...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12141

    authors: Wu CS,Chaw SM

    更新日期:2014-04-01 00:00:00

  • Engineering soya bean seeds as a scalable platform to produce cyanovirin-N, a non-ARV microbicide against HIV.

    abstract::There is an urgent need to provide effective anti-HIV microbicides to resource-poor areas worldwide. Some of the most promising microbicide candidates are biotherapeutics targeting viral entry. To provide biotherapeutics to poorer areas, it is vital to reduce the cost. Here, we report the production of biologically ac...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12309

    authors: O'Keefe BR,Murad AM,Vianna GR,Ramessar K,Saucedo CJ,Wilson J,Buckheit KW,da Cunha NB,Araújo AC,Lacorte CC,Madeira L,McMahon JB,Rech EL

    更新日期:2015-09-01 00:00:00

  • The pomegranate (Punica granatum L.) genome provides insights into fruit quality and ovule developmental biology.

    abstract::Pomegranate (Punica granatum L.) has an ancient cultivation history and has become an emerging profitable fruit crop due to its attractive features such as the bright red appearance and the high abundance of medicinally valuable ellagitannin-based compounds in its peel and aril. However, the limited genomic resources ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12875

    authors: Yuan Z,Fang Y,Zhang T,Fei Z,Han F,Liu C,Liu M,Xiao W,Zhang W,Wu S,Zhang M,Ju Y,Xu H,Dai H,Liu Y,Chen Y,Wang L,Zhou J,Guan D,Yan M,Xia Y,Huang X,Liu D,Wei H,Zheng H

    更新日期:2018-07-01 00:00:00

  • The wheat Em promoter drives reporter gene expression in embryo and aleurone tissue of transgenic barley and rice.

    abstract::The early methionine (Em) proteins are members of the late embryogenesis abundant (LEA) group of proteins that have been considered to be embryo specific. The ability of a 646-bp wheat Em promoter to control green fluorescent protein (gfp) expression was investigated in transgenic barley and rice. Seeds of transgenic ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2005.00135.x

    authors: Furtado A,Henry RJ

    更新日期:2005-07-01 00:00:00

  • Comparison of pathogen-induced expression and efficacy of two amphibian antimicrobial peptides, MsrA2 and temporin A, for engineering wide-spectrum disease resistance in tobacco.

    abstract::The rapid accumulation of defensive transgene products in plants only on pathogen invasion has clear advantages over their constitutive synthesis. In this study, two antimicrobial peptides from the skin secretions of frogs, MsrA2 (N-methionine-dermaseptin B1) and temporin A, were evaluated for engineering pathogen-ind...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2007.00277.x

    authors: Yevtushenko DP,Misra S

    更新日期:2007-11-01 00:00:00

  • Using CRISPR/Cas9 genome editing in tomato to create a gibberellin-responsive dominant dwarf DELLA allele.

    abstract::The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12952

    authors: Tomlinson L,Yang Y,Emenecker R,Smoker M,Taylor J,Perkins S,Smith J,MacLean D,Olszewski NE,Jones JDG

    更新日期:2019-01-01 00:00:00

  • Co-expression of NCED and ALO improves vitamin C level and tolerance to drought and chilling in transgenic tobacco and stylo plants.

    abstract::Abscisic acid (ABA) regulates plant adaptive responses to various environmental stresses, while L-ascorbic acid (AsA) that is also named vitamin C is an important antioxidant and involves in plant stress tolerance and the immune system in domestic animals. Transgenic tobacco (Nicotiana tabacum L.) and stylo [Stylosant...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12374

    authors: Bao G,Zhuo C,Qian C,Xiao T,Guo Z,Lu S

    更新日期:2016-01-01 00:00:00

  • Functional screening of willow alleles in Arabidopsis combined with QTL mapping in willow (Salix) identifies SxMAX4 as a coppicing response gene.

    abstract::Willows (Salix spp.) are important biomass crops due to their ability to grow rapidly with low fertilizer inputs and ease of cultivation in short-rotation coppice cycles. They are relatively undomesticated and highly diverse, but functional testing to identify useful allelic variation is time-consuming in trees and tr...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12154

    authors: Salmon J,Ward SP,Hanley SJ,Leyser O,Karp A

    更新日期:2014-05-01 00:00:00

  • Freedom-to-operate analysis of a transgenic multivitamin corn variety.

    abstract::In this article, we explore the intellectual property (IP) landscape relevant to the production and commercialization of Carolight(™) , a transgenic multivitamin corn variety created on humanitarian grounds to address micronutrient deficiencies in low-and-middle-income countries. The successful production of this vari...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12488

    authors: Zanga D,Capell T,Zhu C,Christou P,Thangaraj H

    更新日期:2016-05-01 00:00:00

  • Gelatinization temperature of rice explained by polymorphisms in starch synthase.

    abstract::The cooking quality of rice is associated with the starch gelatinization temperature (GT). Rice genotypes with low GT have probably been selected for their cooking quality by humans during domestication. We now report polymorphisms in starch synthase IIa (SSIIa) that explain the variation in rice starch GT. Sequence a...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2005.00162.x

    authors: Waters DL,Henry RJ,Reinke RF,Fitzgerald MA

    更新日期:2006-01-01 00:00:00

  • Fusion of an Fc chain to a VHH boosts the accumulation levels in Arabidopsis seeds.

    abstract::Nanobodies® (VHHs) provide powerful tools in therapeutic and biotechnological applications. Nevertheless, for some applications, bivalent antibodies perform much better, and for this, an Fc chain can be fused to the VHH domain, resulting in a bivalent homodimeric VHH-Fc complex. However, the production of bivalent ant...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12094

    authors: De Buck S,Nolf J,De Meyer T,Virdi V,De Wilde K,Van Lerberge E,Van Droogenbroeck B,Depicker A

    更新日期:2013-10-01 00:00:00

  • Simultaneous boosting of source and sink capacities doubles tuber starch yield of potato plants.

    abstract::An important goal in biotechnological research is to improve the yield of crop plants. Here, we genetically modified simultaneously source and sink capacities in potato (Solanum tuberosum cv. Desirée) plants to improve starch yield. Source capacity was increased by mesophyll-specific overexpression of a pyrophosphatas...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2012.00736.x

    authors: Jonik C,Sonnewald U,Hajirezaei MR,Flügge UI,Ludewig F

    更新日期:2012-12-01 00:00:00

  • Overexpression of Hevea brasiliensis ethylene response factor HbERF-IXc5 enhances growth and tolerance to abiotic stress and affects laticifer differentiation.

    abstract::Ethylene response factor 1 (ERF1) is an essential integrator of the jasmonate and ethylene signalling pathways coordinating a large number of genes involved in plant defences. Its orthologue in Hevea brasiliensis, HbERF-IXc5, has been assumed to play a major role in laticifer metabolism and tolerance to harvesting str...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12774

    authors: Lestari R,Rio M,Martin F,Leclercq J,Woraathasin N,Roques S,Dessailly F,Clément-Vidal A,Sanier C,Fabre D,Melliti S,Suharsono S,Montoro P

    更新日期:2018-01-01 00:00:00

  • Molecular analysis of menadione-induced resistance against biotic stress in Arabidopsis.

    abstract::Menadione sodium bisulphite (MSB) is a water-soluble derivative of vitamin K3, or menadione, and has been previously demonstrated to function as a plant defence activator against several pathogens in several plant species. However, there are no reports of the role of this vitamin in the induction of resistance in the ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2009.00439.x

    authors: Borges AA,Dobon A,Expósito-Rodríguez M,Jiménez-Arias D,Borges-Pérez A,Casañas-Sánchez V,Pérez JA,Luis JC,Tornero P

    更新日期:2009-10-01 00:00:00