Abstract:
:Drought is the major environmental factor limiting crop productivity worldwide. We hypothesized that it is possible to enhance drought tolerance by delaying stress-induced senescence through the stress-induced synthesis of cytokinins in crop-plants. We generated transgenic rice (Oryza sativa) plants expressing an isopentenyltransferase (IPT) gene driven by P(SARK) , a stress- and maturation-induced promoter. Plants were tested for drought tolerance at two yield-sensitive developmental stages: pre- and post-anthesis. Under both treatments, the transgenic rice plants exhibited delayed response to stress with significantly higher grain yield (GY) when compared to wild-type plants. Gene expression analysis revealed a significant shift in expression of hormone-associated genes in the transgenic plants. During water-stress (WS), P(SARK)::IPT plants displayed increased expression of brassinosteroid-related genes and repression of jasmonate-related genes. Changes in hormone homeostasis were associated with resource(s) mobilization during stress. The transgenic plants displayed differential expression of genes encoding enzymes associated with hormone synthesis and hormone-regulated pathways. These changes and associated hormonal crosstalk resulted in the modification of source/sink relationships and a stronger sink capacity of the P(SARK)::IPT plants during WS. As a result, the transgenic plants had higher GY with improved quality (nutrients and starch content).
journal_name
Plant Biotechnol Jjournal_title
Plant biotechnology journalauthors
Peleg Z,Reguera M,Tumimbang E,Walia H,Blumwald Edoi
10.1111/j.1467-7652.2010.00584.xsubject
Has Abstractpub_date
2011-09-01 00:00:00pages
747-58issue
7eissn
1467-7644issn
1467-7652journal_volume
9pub_type
杂志文章abstract::Recent developments of tools for targeted genome modification have led to new concepts in how multiple traits can be combined. Targeted genome modification is based on the use of nucleases with tailor-made specificities to introduce a DNA double-strand break (DSB) at specific target loci. A re-engineered meganuclease ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12085
更新日期:2013-10-01 00:00:00
abstract::Over 3.5 million expressed sequence tags from the major cereal taxa were used to electronically mine over 176,000 putative single nucleotide polymorphisms (SNPs). The density, distribution and degree of linkage between these SNPs were compared among the different taxa. The frequency of sequence polymorphism was lowest...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2009.00412.x
更新日期:2009-05-01 00:00:00
abstract::Grain size, number and starch content are important determinants of grain yield and quality. One of the most important biological processes that determine these components is the carbon partitioning during the early grain filling, which requires the function of cell wall invertase. Here, we showed the constitutive exp...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12102
更新日期:2013-12-01 00:00:00
abstract::Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer c...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12359
更新日期:2016-01-01 00:00:00
abstract::Glycine betaine plays an important role in some plants, including maize, in conditions of abiotic stress, but different maize varieties vary in their capacity to accumulate glycine betaine. An elite maize inbred line DH4866 was transformed with the betA gene from Escherichia coli encoding choline dehydrogenase (EC 1.1...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2004.00093.x
更新日期:2004-11-01 00:00:00
abstract::One goal of modern agriculture is the improvement of plant drought tolerance and water-use efficiency (WUE). Although stomatal density has been linked to WUE, the causal molecular mechanisms and engineered alternations of this relationship are not yet fully understood. Moreover, YODA (YDA), which is a MAPKK kinase gen...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12324
更新日期:2015-09-01 00:00:00
abstract::Transgenic engineering of plants is important in both basic and applied research. However, the expression of a transgene can dwindle over time as the plant's small (s)RNA-guided silencing pathways shut it down. The silencing pathways have evolved as antiviral defence mechanisms, and viruses have co-evolved viral silen...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12506
更新日期:2016-06-01 00:00:00
abstract::Methods based on single nucleotide polymorphism (SNP), copy number variation (CNV) and presence/absence variation (PAV) discovery provide a valuable resource to study gene structure and evolution. However, as a result of these structural variations, a single reference genome is unable to cover the entire gene content ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13262
更新日期:2020-04-01 00:00:00
abstract::Woody biomass has gained popularity as an environmentally friendly, renewable and sustainable resource for liquid fuel production. Here, we demonstrate biotechnological improvement of the quantity and quality of woody biomass by employing developing xylem (DX)-preferential production of gibberellin (GA), a phytohormon...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12484
更新日期:2016-04-01 00:00:00
abstract::Zinc-finger nucleases (ZFNs) are artificial restriction enzymes, custom designed for induction of double-strand breaks (DSBs) at a specific locus. These DSBs may result in site-specific mutagenesis or homologous recombination at the repair site, depending on the DNA repair pathway that is used. These promising techniq...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2009.00446.x
更新日期:2009-10-01 00:00:00
abstract::Abscisic acid (ABA) regulates plant adaptive responses to various environmental stresses, while L-ascorbic acid (AsA) that is also named vitamin C is an important antioxidant and involves in plant stress tolerance and the immune system in domestic animals. Transgenic tobacco (Nicotiana tabacum L.) and stylo [Stylosant...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12374
更新日期:2016-01-01 00:00:00
abstract::Approximately 20,000 of the rice-FOX Arabidopsis transgenic lines, which overexpress 13,000 rice full-length cDNAs at random in Arabidopsis, were screened for bacterial disease resistance by dip inoculation with Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). The identities of the overexpressed genes were determi...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2010.00568.x
更新日期:2011-05-01 00:00:00
abstract::Transgenic rice accumulating the modified major Japanese cedar pollen allergens, Cryptomeria japonica 1 (Cry j 1) and Cryptomeria japonica 2 (Cry j 2), which were deconstructed by fragmentation and shuffling, respectively, in the edible part of the seed was generated by transformation of a good-tasting rice variety, '...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12007
更新日期:2013-01-01 00:00:00
abstract::Advances in the genetic transformation of cereals have improved the prospects of using biotechnology for plant improvement, and a toolbox of promoters with defined specificities would be a valuable resource in controlling the expression of transgenes in desired tissues for both plant improvement and molecular farming....
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2008.00394.x
更新日期:2009-04-01 00:00:00
abstract::Heavy metal accumulation in the environment poses great risks to flora and fauna. However, monitoring sites prone to accumulation poses scale and economic challenges. In this study, we present and test a method for monitoring these sites using fluorescent resonance energy transfer (FRET) change in response to zinc (Zn...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2011.00656.x
更新日期:2012-02-01 00:00:00
abstract::The importance and urgency of providing humans and animals with quality proteins are reflected in the growing scientific and industrial interest in augmenting the nutritive value of the world's protein sources. Such nutritive value is determined by the protein content in 'essential amino acids', those that cannot be s...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/j.1467-7652.2006.00204.x
更新日期:2006-09-01 00:00:00
abstract::GM crops are the most studied crops in history. Approximately 5% of the safety studies on them show adverse effects that are a cause for concern and tend to be featured in media reports. Although these reports are based on just a handful of GM events, they are used to cast doubt on all GM crops. Furthermore, they tend...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/pbi.12798
更新日期:2017-10-01 00:00:00
abstract::Anthocyanins have crucial biological functions and affect quality of horticultural produce. Anthocyanins accumulate in ripe peach fruit; differential accumulation is observed in deep coloured cultivar 'Hujingmilu' and lightly pigmented cultivar 'Yulu'. The difference was not fully explained by accumulation of total fl...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13291
更新日期:2020-05-01 00:00:00
abstract::Menadione sodium bisulphite (MSB) is a water-soluble derivative of vitamin K3, or menadione, and has been previously demonstrated to function as a plant defence activator against several pathogens in several plant species. However, there are no reports of the role of this vitamin in the induction of resistance in the ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2009.00439.x
更新日期:2009-10-01 00:00:00
abstract::Transcription factors have been shown to control the activity of multiple stress response genes in a coordinated manner and therefore represent attractive targets for application in molecular plant breeding. We investigated the possibility of modulating the transcriptional regulation of drought and cold responses in t...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2010.00547.x
更新日期:2011-02-01 00:00:00
abstract::Genetic engineering with just a few genes has changed agriculture in the last 20 years. The most frequently used transgenes are the herbicide resistance genes for efficient weed control and the Bt toxin genes for insect resistance. The adoption of the first-generation genetically engineered crops has been very success...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/pbi.12466
更新日期:2016-05-01 00:00:00
abstract::A new understanding of leaf starch degradation has emerged in the last 10 years. It has been shown that starch phosphorylation and dephosphorylation are critical components of this process. Glucan, water dikinase (GWD) (and phosphoglucan, water dikinase) adds phosphate to starch, and phosphoglucan phosphatase (SEX4) r...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2012.00684.x
更新日期:2012-06-01 00:00:00
abstract::Eastern cottonwood (Populus deltoides Bartr. ex Marsh.) trees were engineered to express merA (mercuric ion reductase) and merB (organomercury lyase) transgenes in order to be used for the phytoremediation of mercury-contaminated soils. Earlier studies with Arabidopsis thaliana and Nicotiana tabacum showed that this g...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2006.00236.x
更新日期:2007-03-01 00:00:00
abstract::Broad spectrum protection against different insects and pathogens requires multigene engineering. However, such broad spectrum protection against biotic stress is provided by a single protein in some medicinal plants. Therefore, tobacco chloroplasts were transformed with the agglutinin gene from Pinellia ternata (pta)...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2011.00663.x
更新日期:2012-04-01 00:00:00
abstract::Downy mildew (Plasmopara halstedii (Farl.) Berlese et de Toni) is a serious foliar pathogen of cultivated sunflower (Helianthus annuus L.). Genetic resistance is conditioned by several linked downy mildew resistance gene specificities in the HaRGC1 cluster of TIR-NBS-LRR resistance gene candidates (RGCs) on linkage gr...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1046/j.1467-7652.2003.00016.x
更新日期:2003-05-01 00:00:00
abstract::The rice α-amylase 3D promoter system, which is activated under sucrose-starved conditions, has emerged as a useful system for producing recombinant proteins. However, using rice as the production system for therapeutic proteins requires modifications of the N-glycosylation pattern because of the potential immunogenic...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2011.00636.x
更新日期:2011-12-01 00:00:00
abstract::For future food security, it is important that wheat, one of the most widely consumed crops in the world, can survive the threat of abiotic and biotic stresses. New genetic variation is currently being introduced into wheat through introgressions from its wild relatives. For trait discovery, it is necessary that each ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13241
更新日期:2020-03-01 00:00:00
abstract::Single-chain variable fragments (scFvs), as well as other recombinant antibodies, have become powerful tools for therapy and analysis in both human and veterinary medicine. Transgenic plants have been developed as an efficient production system for these recombinant proteins, achieving high yields at moderate cost. Se...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2005.00176.x
更新日期:2006-03-01 00:00:00
abstract::Expression of the cecropin A gene in rice confers resistance to the rice blast fungus Magnaporthe oryzae. In this study, a polymerase chain reaction-based suppression subtractive hybridization approach was used to generate a cDNA macroarray from the elite japonica rice (Oryza sativa L.) cultivar 'Senia'. Gene expressi...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2008.00339.x
更新日期:2008-08-01 00:00:00
abstract::The tissue-specific expression of transgenes is essential in plant breeding programmes to avoid the fitness costs caused by constitutive expression of a target gene. However, knowledge on the molecular mechanisms of tissue-specific gene expression and practicable tissue-specific promoters is limited. In this study, we...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2007.00271.x
更新日期:2007-09-01 00:00:00