Abstract:
:Increased use of nitrogen fertilizers has deleterious impact on the environment. Increase in yield potential at low nitrogen supply is regarded as a cereal breeding goal for future agricultural sustainability. Although natural variations of nitrogen transporters have been investigated, key genes associated with assimilation remain largely unexplored for nitrogen use efficiency (NUE) enhancement. Here, we identified a NIN-like protein NLP4 associated with NUE through a GWAS in rice. We found that OsNLP4 transactivated OsNiR encoding nitrite reductase that was critical in nitrogen assimilation in rice. We further constructed quadrupling NREs (Nitrate-responsive cis-elements) in the promoter of OsNiR (p4xNRE:OsNiR) and enhanced nitrogen assimilation significantly. We demonstrated that OsNLP4-OsNiR increased tiller number and yield through enhancing nitrogen assimilation and NUE. Our discovery highlights the genetic modulation of OsNLP4-OsNiR signalling cascade as a strategy for high NUE and yield breeding in rice.
journal_name
Plant Biotechnol Jjournal_title
Plant biotechnology journalauthors
Yu J,Xuan W,Tian Y,Fan L,Sun J,Tang W,Chen G,Wang B,Liu Y,Wu W,Liu X,Jiang X,Zhou C,Dai Z,Xu D,Wang C,Wan Jdoi
10.1111/pbi.13450subject
Has Abstractpub_date
2021-01-01 00:00:00pages
167-176issue
1eissn
1467-7644issn
1467-7652journal_volume
19pub_type
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pub_type: 杂志文章,随机对照试验
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