Plant-produced candidate countermeasures against emerging and reemerging infections and bioterror agents.

abstract：:Which factors limit metabolite accumulation in plant cells? Are theories on flux control effective at explaining the results? Many biotechnologists cling to the idea that every pathway has a rate limiting enzyme and target such enzymes first in order to modulate fluxes. This often translates into large effects on meta...

journal_title：Plant biotechnology journal

authors： Morandini P

更新日期：2013-02-01 00:00:00

abstract：:Nanobodies® (VHHs) provide powerful tools in therapeutic and biotechnological applications. Nevertheless, for some applications, bivalent antibodies perform much better, and for this, an Fc chain can be fused to the VHH domain, resulting in a bivalent homodimeric VHH-Fc complex. However, the production of bivalent ant...

journal_title：Plant biotechnology journal

authors： De Buck S,Nolf J,De Meyer T,Virdi V,De Wilde K,Van Lerberge E,Van Droogenbroeck B,Depicker A

更新日期：2013-10-01 00:00:00

abstract：:Advances in the genetic transformation of cereals have improved the prospects of using biotechnology for plant improvement, and a toolbox of promoters with defined specificities would be a valuable resource in controlling the expression of transgenes in desired tissues for both plant improvement and molecular farming....

journal_title：Plant biotechnology journal

authors： Furtado A,Henry RJ,Pellegrineschi A

更新日期：2009-04-01 00:00:00

abstract：:Peanut allergy is an IgE-mediated adverse reaction to a subset of proteins found in peanuts. Immunotherapy aims to desensitize allergic patients through repeated and escalating exposures for several months to years using extracts or flours. The complex mix of proteins and variability between preparations complicates i...

journal_title：Plant biotechnology journal

authors： Gregory JA,Shepley-McTaggart A,Umpierrez M,Hurlburt BK,Maleki SJ,Sampson HA,Mayfield SP,Berin MC

更新日期：2016-07-01 00:00:00

abstract：:The rice α-amylase 3D promoter system, which is activated under sucrose-starved conditions, has emerged as a useful system for producing recombinant proteins. However, using rice as the production system for therapeutic proteins requires modifications of the N-glycosylation pattern because of the potential immunogenic...

journal_title：Plant biotechnology journal

authors： Shin YJ,Chong YJ,Yang MS,Kwon TH

更新日期：2011-12-01 00:00:00

abstract：:For future food security, it is important that wheat, one of the most widely consumed crops in the world, can survive the threat of abiotic and biotic stresses. New genetic variation is currently being introduced into wheat through introgressions from its wild relatives. For trait discovery, it is necessary that each ...

journal_title：Plant biotechnology journal

authors： Grewal S,Hubbart-Edwards S,Yang C,Devi U,Baker L,Heath J,Ashling S,Scholefield D,Howells C,Yarde J,Isaac P,King IP,King J

更新日期：2020-03-01 00:00:00

abstract：:Pollen germination and pollen tube growth are important physiological processes of sexual reproduction of plants and also are involved in signal transduction. Our previous study reveals that ZmSTK1 and ZmSTK2 are two receptor-like cytoplasmic kinases (RLCK) homologs in Zea mays as members of receptor-like protein kina...

journal_title：Plant biotechnology journal

authors： Fan M,Zhang C,Shi L,Liu C,Ma W,Chen M,Liu K,Cai F,Wang G,Wei Z,Jiang M,Liu Z,Javeed A,Lin F

更新日期：2018-08-01 00:00:00

abstract：:N-glycosylation is critical for recombinant glycoprotein production as it influences the heterogeneity of products and affects their biological function. In most eukaryotes, the oligosaccharyltransferase is the central-protein complex facilitating the N-glycosylation of proteins in the lumen of the endoplasmic reticul...

journal_title：Plant biotechnology journal

authors： Castilho A,Beihammer G,Pfeiffer C,Göritzer K,Montero-Morales L,Vavra U,Maresch D,Grünwald-Gruber C,Altmann F,Steinkellner H,Strasser R

更新日期：2018-10-01 00:00:00

abstract：:Abscisic acid (ABA) regulates plant adaptive responses to various environmental stresses, while L-ascorbic acid (AsA) that is also named vitamin C is an important antioxidant and involves in plant stress tolerance and the immune system in domestic animals. Transgenic tobacco (Nicotiana tabacum L.) and stylo [Stylosant...

journal_title：Plant biotechnology journal

authors： Bao G,Zhuo C,Qian C,Xiao T,Guo Z,Lu S

更新日期：2016-01-01 00:00:00

abstract：:Lutein and zeaxanthin cannot be synthesized de novo in humans, and although lutein is abundant in fruit and vegetables, good dietary sources of zeaxanthin are scarce. Certain corn varieties provide adequate amounts because the ratio of endosperm β:ε lycopene cyclase activity favours the β-carotene/zeaxanthin branch of...

journal_title：Plant biotechnology journal

authors： Naqvi S,Zhu C,Farre G,Sandmann G,Capell T,Christou P

更新日期：2011-04-01 00:00:00

abstract：:Next-generation sequencing technologies provide opportunities to understand the genetic basis of phenotypic differences, such as abiotic stress response, even in the closely related cultivars via identification of large number of DNA polymorphisms. We performed whole-genome resequencing of three rice cultivars with co...

journal_title：Plant biotechnology journal

authors： Jain M,Moharana KC,Shankar R,Kumari R,Garg R

更新日期：2014-02-01 00:00:00

abstract：:Expression of industrial enzymes in transgenic plants offers an alternative system to fungal fermentation for large-scale production. Very high levels of expression are required to make the enzymes cost-effective. We tested several parameters to determine the best method for achieving high levels of expression for a f...

journal_title：Plant biotechnology journal

authors： Hood EE,Bailey MR,Beifuss K,Magallanes-Lundback M,Horn ME,Callaway E,Drees C,Delaney DE,Clough R,Howard JA

更新日期：2003-03-01 00:00:00

abstract：:Soya bean (Glycine max) and grass pea (Lathyrus sativus) seeds are important sources of dietary proteins; however, they also contain antinutritional metabolite oxalic acid (OA). Excess dietary intake of OA leads to nephrolithiasis due to the formation of calcium oxalate crystals in kidneys. Besides, OA is also a known...

journal_title：Plant biotechnology journal

authors： Kumar V,Chattopadhyay A,Ghosh S,Irfan M,Chakraborty N,Chakraborty S,Datta A

更新日期：2016-06-01 00:00:00

abstract：:Triterpenes are thirty-carbon compounds derived from the universal five-carbon prenyl precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Normally, triterpenes are synthesized via the mevalonate (MVA) pathway operating in the cytoplasm of eukaryotes where DMAPP is condensed with two IPPs to...

journal_title：Plant biotechnology journal

authors： Kempinski C,Jiang Z,Zinck G,Sato SJ,Ge Z,Clemente TE,Chappell J

更新日期：2019-02-01 00:00:00

abstract：:Whole-genome sequencing-based bulked segregant analysis (BSA) for mapping quantitative trait loci (QTL) provides an efficient alternative approach to conventional QTL analysis as it significantly reduces the scale and cost of analysis with comparable power to QTL detection using full mapping population. We tested the ...

journal_title：Plant biotechnology journal

authors： Deokar A,Sagi M,Daba K,Tar'an B

更新日期：2019-01-01 00:00:00

abstract：:Orobanche spp. (broomrape) are parasitic plants which subsist on the roots of a wide range of hosts, including tomato, causing severe losses in yield quality and quantity. Large amounts of mannitol accumulate in this parasitic weed during development. Mannose 6-phosphate reductase (M6PR) is a key enzyme in mannitol bi...

journal_title：Plant biotechnology journal

authors： Aly R,Cholakh H,Joel DM,Leibman D,Steinitz B,Zelcer A,Naglis A,Yarden O,Gal-On A

更新日期：2009-08-01 00:00:00

abstract：:In this article, we explore the intellectual property (IP) landscape relevant to the production and commercialization of Carolight(™) , a transgenic multivitamin corn variety created on humanitarian grounds to address micronutrient deficiencies in low-and-middle-income countries. The successful production of this vari...

journal_title：Plant biotechnology journal

authors： Zanga D,Capell T,Zhu C,Christou P,Thangaraj H

更新日期：2016-05-01 00:00:00

abstract：:Drought tolerance of the wheat cultivar Bobwhite was previously enhanced by transformation with a construct containing the wheat DREB3 gene driven by the stress-inducible maize Rab17 promoter. Progeny of a single T2 transgenic line were used as pollinators in crosses with four elite bread wheat cultivars from Western ...

journal_title：Plant biotechnology journal

authors： Shavrukov Y,Baho M,Lopato S,Langridge P

更新日期：2016-01-01 00:00:00

abstract：:We constructed a novel autonomously replicating gene expression shuttle vector, with the aim of developing a system for transiently expressing proteins at levels useful for commercial production of vaccines and other proteins in plants. The vector, pRIC, is based on the mild strain of the geminivirus Bean yellow dwarf...

journal_title：Plant biotechnology journal

authors： Regnard GL,Halley-Stott RP,Tanzer FL,Hitzeroth II,Rybicki EP

更新日期：2010-01-01 00:00:00

abstract：:Grain size and plant architecture are critical factors determining crop productivity. Here, we performed gene editing of the MIR396 gene family in rice and found that MIR396e and MIR396f are two important regulators of grain size and plant architecture. mir396ef mutations can increase grain yield by increasing grain s...

journal_title：Plant biotechnology journal

authors： Miao C,Wang D,He R,Liu S,Zhu JK

更新日期：2020-02-01 00:00:00

abstract：:Considered responsible for one million deaths in Ireland and widespread famine in the European continent during the 1840s, late blight, caused by Phytophthora infestans, remains the most devastating disease of potato (Solanum tuberosum L.) with about 15%-30% annual yield loss in sub-Saharan Africa, affecting mainly sm...

journal_title：Plant biotechnology journal

authors： Ghislain M,Byarugaba AA,Magembe E,Njoroge A,Rivera C,Román ML,Tovar JC,Gamboa S,Forbes GA,Kreuze JF,Barekye A,Kiggundu A

更新日期：2019-06-01 00:00:00

abstract：:Drought is the major environmental factor limiting crop productivity worldwide. We hypothesized that it is possible to enhance drought tolerance by delaying stress-induced senescence through the stress-induced synthesis of cytokinins in crop-plants. We generated transgenic rice (Oryza sativa) plants expressing an isop...

journal_title：Plant biotechnology journal

authors： Peleg Z,Reguera M,Tumimbang E,Walia H,Blumwald E

更新日期：2011-09-01 00:00:00

abstract：:The feasibility of using the FLP/FRT site-specific recombination system in rice for genome engineering was evaluated. Transgenic rice plants expressing the FLP recombinase were crossed with plants harbouring the kanamycin resistance gene (neomycin phosphotransferase II, nptII) flanked by FRT sites, which also served t...

journal_title：Plant biotechnology journal

authors： Hu Q,Kononowicz-Hodges H,Nelson-Vasilchik K,Viola D,Zeng P,Liu H,Kausch AP,Chandlee JM,Hodges TK,Luo H

更新日期：2008-02-01 00:00:00

abstract：:With the aim of increasing the methionine level in alfalfa (Medicago sativa L.) and thus improving its nutritional quality, we produced transgenic alfalfa plants that expressed the Arabidopsis cystathionine gamma-synthase (AtCGS), the enzyme that controls the synthesis of the first intermediate metabolite in the methi...

journal_title：Plant biotechnology journal

authors： Avraham T,Badani H,Galili S,Amir R

更新日期：2005-01-01 00:00:00

abstract：:Genome modification by homology-directed repair (HDR) is an attractive tool for the controlled genetic manipulation of plants. Here, we report the HDR-mediated gene exchange of expression cassettes in tobacco BY-2 cells using a designed zinc finger nuclease (ZFN). The target contained a 7-kb fragment flanked by two ZF...

journal_title：Plant biotechnology journal

authors： Schneider K,Schiermeyer A,Dolls A,Koch N,Herwartz D,Kirchhoff J,Fischer R,Russell SM,Cao Z,Corbin DR,Sastry-Dent L,Ainley WM,Webb SR,Schinkel H,Schillberg S

更新日期：2016-04-01 00:00:00

abstract：:The nitrate (NO3-) transporter has been selected as an important gene maker in the process of environmental adoption in rice cultivars. In this work, we transferred another native OsNAR2.1 promoter with driving OsNAR2.1 gene into rice plants. The transgenic lines with exogenous pOsNAR2.1:OsNAR2.1 constructs showed enh...

journal_title：Plant biotechnology journal

authors： Chen J,Fan X,Qian K,Zhang Y,Song M,Liu Y,Xu G,Fan X

更新日期：2017-10-01 00:00:00

abstract：:A hybrid-proline-rich protein encoding gene (CcHyPRP) has been isolated and characterized, for the first time, from the subtracted cDNA library of pigeonpea (Cajanus cajan L.) plants subjected to drought stress. Functionality of CcHyPRP has been validated for abiotic stress tolerance using the heterologous yeast and A...

journal_title：Plant biotechnology journal

authors： Priyanka B,Sekhar K,Reddy VD,Rao KV

更新日期：2010-01-01 00:00:00

abstract：SUMMARY:Compared with other plant expression systems used for pharmaceutical protein production, alfalfa offers the advantage of very homogeneous N-glycosylation. Therefore, this plant was selected for further attempts at glycoengineering. Two main approaches were developed in order to humanize N-glycosylation in alfal...

journal_title：Plant biotechnology journal

authors： Sourrouille C,Marquet-Blouin E,D'Aoust MA,Kiefer-Meyer MC,Seveno M,Pagny-Salehabadi S,Bardor M,Durambur G,Lerouge P,Vezina L,Gomord V

更新日期：2008-09-01 00:00:00

abstract：:Tef, Eragrostis tef (Zucc.) Trotter, is the most important cereal in Ethiopia. Tef is cultivated by more than five million small-scale farmers annually and constitutes the staple food for more than half of the population of 80 million. The crop is preferred by both farmers and consumers due to its beneficial traits as...

journal_title：Plant biotechnology journal

authors： Girma D,Assefa K,Chanyalew S,Cannarozzi G,Kuhlemeier C,Tadele Z

更新日期：2014-06-01 00:00:00

abstract：:MicroRNAs (miRNAs) are a class of regulatory small RNAs (sRNAs) that down-regulate target genes through mRNA cleavage or translational inhibition. miRNA is known to play an important role in the root development and environmental responses in both the Arabidopsis and rice. However, little information is available to f...

journal_title：Plant biotechnology journal

authors： Kong X,Zhang M,Xu X,Li X,Li C,Ding Z

更新日期：2014-10-01 00:00:00