Abstract:
:Which factors limit metabolite accumulation in plant cells? Are theories on flux control effective at explaining the results? Many biotechnologists cling to the idea that every pathway has a rate limiting enzyme and target such enzymes first in order to modulate fluxes. This often translates into large effects on metabolite concentration, but disappointing small increases in flux. Rate limiting enzymes do exist, but are rare and quite opposite to what predicted by biochemistry. In many cases however, flux control is shared among many enzymes. Flux control and concentration control can (and must) be distinguished and quantified for effective manipulation. Flux control for several 'building blocks' of metabolism is placed on the demand side, and therefore increasing demand can be very successful. Tampering with supply, particularly desensitizing supply enzymes, is usually not very effective, if not dangerous, because supply regulatory mechanisms function to control metabolite homeostasis. Some important, but usually unnoticed, metabolic constraints shape the responses of metabolic systems to manipulation: mass conservation, cellular resource allocation and, most prominently, energy supply, particularly in heterotrophic tissues. The theoretical basis for this view shall be explored with recent examples gathered from the manipulation of several metabolites (vitamins, carotenoids, amino acids, sugars, fatty acids, polyhydroxyalkanoates, fructans and sugar alcohols). Some guiding principles are suggested for an even more successful engineering of plant metabolism.
journal_name
Plant Biotechnol Jjournal_title
Plant biotechnology journalauthors
Morandini Pdoi
10.1111/pbi.12035subject
Has Abstractpub_date
2013-02-01 00:00:00pages
253-67issue
2eissn
1467-7644issn
1467-7652journal_volume
11pub_type
杂志文章,评审abstract::Next-generation sequencing technologies provide opportunities to understand the genetic basis of phenotypic differences, such as abiotic stress response, even in the closely related cultivars via identification of large number of DNA polymorphisms. We performed whole-genome resequencing of three rice cultivars with co...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12133
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abstract::Eastern cottonwood (Populus deltoides Bartr. ex Marsh.) trees were engineered to express merA (mercuric ion reductase) and merB (organomercury lyase) transgenes in order to be used for the phytoremediation of mercury-contaminated soils. Earlier studies with Arabidopsis thaliana and Nicotiana tabacum showed that this g...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2006.00236.x
更新日期:2007-03-01 00:00:00
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journal_title:Plant biotechnology journal
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journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13086
更新日期:2019-08-01 00:00:00
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journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2007.00277.x
更新日期:2007-11-01 00:00:00
abstract::Steviol glycosides (SGs) are extracted from Stevia leaves for use as a natural sweetener. Among SGs, stevioside is most abundant in leaf extracts followed by rebaudioside A (Reb A). However, Reb A is of particular interest because of its sweeter and more pleasant taste compared to stevioside. Therefore, the developmen...
journal_title:Plant biotechnology journal
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doi:10.1111/pbi.13035
更新日期:2019-06-01 00:00:00
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journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12714
更新日期:2017-10-01 00:00:00
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journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12896
更新日期:2018-03-05 00:00:00
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journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2012.00684.x
更新日期:2012-06-01 00:00:00
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journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13078
更新日期:2019-08-01 00:00:00
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journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13226
更新日期:2020-02-01 00:00:00
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journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/j.1467-7652.2007.00315.x
更新日期:2008-02-01 00:00:00
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journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12795
更新日期:2018-02-01 00:00:00
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journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12094
更新日期:2013-10-01 00:00:00
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journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1046/j.1467-7652.2003.00045.x
更新日期:2004-01-01 00:00:00
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journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12515
更新日期:2016-07-01 00:00:00
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journal_title:Plant biotechnology journal
pub_type: 杂志文章
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更新日期:2013-04-01 00:00:00
abstract::The plant pathogen, Pseudomonas syringae (Ps), together with related Ps species, infects and attacks a wide range of agronomically important crops, including tomato, kiwifruit, pepper, olive and soybean, causing economic losses. Currently, chemicals and introduced resistance genes are used to protect plants against th...
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更新日期:2020-05-01 00:00:00
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journal_title:Plant biotechnology journal
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journal_title:Plant biotechnology journal
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journal_title:Plant biotechnology journal
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更新日期:2011-09-01 00:00:00
abstract::Whole-genome sequencing-based bulked segregant analysis (BSA) for mapping quantitative trait loci (QTL) provides an efficient alternative approach to conventional QTL analysis as it significantly reduces the scale and cost of analysis with comparable power to QTL detection using full mapping population. We tested the ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
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更新日期:2019-01-01 00:00:00
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journal_title:Plant biotechnology journal
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journal_title:Plant biotechnology journal
pub_type: 杂志文章
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journal_title:Plant biotechnology journal
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journal_title:Plant biotechnology journal
pub_type: 杂志文章
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更新日期:2018-02-01 00:00:00
abstract::Recent developments of tools for targeted genome modification have led to new concepts in how multiple traits can be combined. Targeted genome modification is based on the use of nucleases with tailor-made specificities to introduce a DNA double-strand break (DSB) at specific target loci. A re-engineered meganuclease ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
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