Analysis of promoters in transgenic barley and wheat.

Abstract:

:Advances in the genetic transformation of cereals have improved the prospects of using biotechnology for plant improvement, and a toolbox of promoters with defined specificities would be a valuable resource in controlling the expression of transgenes in desired tissues for both plant improvement and molecular farming. A number of promoters have been isolated from the important cereals (wheat, barley, rice and maize), and these promoters have been tested mostly in homologous cereal systems and, to a lesser extent, in heterologous cereal systems. The use of these promoters across the important cereals would add value to the utility of each promoter. In addition, promoters with less sequence homology, but with similar specificities, will be crucial in avoiding homology-based gene silencing when expressing more than one transgene in the same tissue. We have tested wheat and barley promoters in transgenic barley and wheat to determine whether their specificity is shared across these two species. The barley bifunctional alpha-amylase/subtilisin inhibitor (Isa) promoter, specific to the pericarp in barley, failed to show any activity in wheat, whereas the wheat early-maturing (Em) promoter showed similar activity in wheat and barley. The wheat high-molecular-weight glutenin (HMW-Glu) and barley D-hordein (D-Hor) and B-hordein (B-Hor) storage protein promoters maintained endosperm-specific expression of green fluorescent protein (GFP) in wheat and barley, respectively. Using gfp, we have demonstrated that the Isa and Em promoters can be used as strong promoters to direct transgenes in specific tissues of barley and wheat grain. Differential promoter activity across cereals expands and adds value to a promoter toolbox for utility in plant biotechnology.

journal_name

Plant Biotechnol J

authors

Furtado A,Henry RJ,Pellegrineschi A

doi

10.1111/j.1467-7652.2008.00394.x

subject

Has Abstract

pub_date

2009-04-01 00:00:00

pages

240-53

issue

3

eissn

1467-7644

issn

1467-7652

pii

PBI394

journal_volume

7

pub_type

杂志文章
  • Screening for resistance against Pseudomonas syringae in rice-FOX Arabidopsis lines identified a putative receptor-like cytoplasmic kinase gene that confers resistance to major bacterial and fungal pathogens in Arabidopsis and rice.

    abstract::Approximately 20,000 of the rice-FOX Arabidopsis transgenic lines, which overexpress 13,000 rice full-length cDNAs at random in Arabidopsis, were screened for bacterial disease resistance by dip inoculation with Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). The identities of the overexpressed genes were determi...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2010.00568.x

    authors: Dubouzet JG,Maeda S,Sugano S,Ohtake M,Hayashi N,Ichikawa T,Kondou Y,Kuroda H,Horii Y,Matsui M,Oda K,Hirochika H,Takatsuji H,Mori M

    更新日期:2011-05-01 00:00:00

  • Characterization of disease resistance genes in the Brassica napus pangenome reveals significant structural variation.

    abstract::Methods based on single nucleotide polymorphism (SNP), copy number variation (CNV) and presence/absence variation (PAV) discovery provide a valuable resource to study gene structure and evolution. However, as a result of these structural variations, a single reference genome is unable to cover the entire gene content ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13262

    authors: Dolatabadian A,Bayer PE,Tirnaz S,Hurgobin B,Edwards D,Batley J

    更新日期:2020-04-01 00:00:00

  • Commercialization of new biotechnology: a systematic review of 16 commercial case studies in a novel manufacturing sector.

    abstract::The 1980s and 1990s saw a major expansion of biotechnology into new areas of science including genomics and recombinant technologies. This was coupled to the widespread emergence of academics into the commercial sector as they were encouraged to spin out companies or commercialize their intellectual property. There we...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/pbi.12426

    authors: Paul MJ,Thangaraj H,Ma JK

    更新日期:2015-10-01 00:00:00

  • Haplotype-based genotyping-by-sequencing in oat genome research.

    abstract::In a de novo genotyping-by-sequencing (GBS) analysis of short, 64-base tag-level haplotypes in 4657 accessions of cultivated oat, we discovered 164741 tag-level (TL) genetic variants containing 241224 SNPs. From this, the marker density of an oat consensus map was increased by the addition of more than 70000 loci. The...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12888

    authors: Bekele WA,Wight CP,Chao S,Howarth CJ,Tinker NA

    更新日期:2018-08-01 00:00:00

  • Mutations in MIR396e and MIR396f increase grain size and modulate shoot architecture in rice.

    abstract::Grain size and plant architecture are critical factors determining crop productivity. Here, we performed gene editing of the MIR396 gene family in rice and found that MIR396e and MIR396f are two important regulators of grain size and plant architecture. mir396ef mutations can increase grain yield by increasing grain s...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13214

    authors: Miao C,Wang D,He R,Liu S,Zhu JK

    更新日期:2020-02-01 00:00:00

  • Fitness of Bt-resistant cabbage loopers on Bt cotton plants.

    abstract::Development of resistance to the insecticidal toxins from Bacillus thuringiensis (Bt) in insects is the major threat to the continued success of transgenic Bt crops in agriculture. The fitness of Bt-resistant insects on Bt and non-Bt plants is a key parameter that determines the development of Bt resistance in insect ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12718

    authors: Tetreau G,Wang R,Wang P

    更新日期:2017-10-01 00:00:00

  • Metabolically engineered soybean seed with enhanced threonine levels: biochemical characterization and seed-specific expression of lysine-insensitive variants of aspartate kinases from the enteric bacterium Xenorhabdus bovienii.

    abstract::Threonine (Thr) is one of a few limiting essential amino acids (EAAs) in the animal feed industry, and its level in feed rations can impact production of important meat sources, such as swine and poultry. Threonine as well as EAAs lysine (Lys) and methionine (Met) are all synthesized via the aspartate family pathway. ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2010.00545.x

    authors: Qi Q,Huang J,Crowley J,Ruschke L,Goldman BS,Wen L,Rapp WD

    更新日期:2011-02-01 00:00:00

  • High-Spatiotemporal-Resolution Transcriptomes Provide Insights into Fruit Development and Ripening in Citrus sinensis.

    abstract::Citrus fruit has a unique structure with soft leathery peel and pulp containing vascular bundles and several segments with many juice sacs. The function and morphology of each fruit tissue are different. Therefore, analysis at the organ-wide or mixed-tissue level inevitably obscures many tissue-specific phenomena. Hig...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13549

    authors: Feng G,Wu J,Xu Y,Lu L,Yi H

    更新日期:2021-01-20 00:00:00

  • High-resolution expression profiling of selected gene sets during plant immune activation.

    abstract::The plant immune system involves detection of pathogens via both cell-surface and intracellular receptors. Both receptor classes can induce transcriptional reprogramming that elevates disease resistance. To assess differential gene expression during plant immunity, we developed and deployed quantitative sequence captu...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13327

    authors: Ding P,Ngou BPM,Furzer OJ,Sakai T,Shrestha RK,MacLean D,Jones JDG

    更新日期:2020-07-01 00:00:00

  • CRISPR/Cas9-mediated knockout of Ms1 enables the rapid generation of male-sterile hexaploid wheat lines for use in hybrid seed production.

    abstract::The development and adoption of hybrid seed technology have led to dramatic increases in agricultural productivity. However, it has been a challenge to develop a commercially viable platform for the production of hybrid wheat (Triticum aestivum) seed due to wheat's strong inbreeding habit. Recently, a novel platform f...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13106

    authors: Okada A,Arndell T,Borisjuk N,Sharma N,Watson-Haigh NS,Tucker EJ,Baumann U,Langridge P,Whitford R

    更新日期:2019-10-01 00:00:00

  • ZFN-induced mutagenesis and gene-targeting in Arabidopsis through Agrobacterium-mediated floral dip transformation.

    abstract::Zinc-finger nucleases (ZFNs) are artificial restriction enzymes, custom designed for induction of double-strand breaks (DSBs) at a specific locus. These DSBs may result in site-specific mutagenesis or homologous recombination at the repair site, depending on the DNA repair pathway that is used. These promising techniq...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2009.00446.x

    authors: de Pater S,Neuteboom LW,Pinas JE,Hooykaas PJ,van der Zaal BJ

    更新日期:2009-10-01 00:00:00

  • An AGAMOUS intron-driven cytotoxin leads to flowerless tobacco and produces no detrimental effects on vegetative growth of either tobacco or poplar.

    abstract::Flowerless trait is highly desirable for poplar because it can prevent pollen- and seed-mediated transgene flow. We have isolated the second intron of PTAG2, an AGAMOUS (AG) orthologue from Populus trichocarpa. By fusing this intron sequence to a minimal 35S promoter sequence, we created two artificial promoters, fPTA...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12581

    authors: Li W,Hu W,Fang C,Chen L,Zhuang W,Katin-Grazzini L,McAvoy RJ,Guillard K,Li Y

    更新日期:2016-12-01 00:00:00

  • Opinion piece: genomics and crop plant science in Europe.

    abstract::Recent report reviews and funding initiatives in the field of plant genomic research are considered in the context of their translation into practical and economic value via plant breeding. It is concluded that there is a deficit in investment and that a change in working styles towards knowledge sharing and connectiv...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2005.00164.x

    authors: Hughes S

    更新日期:2006-01-01 00:00:00

  • Transient expression assays in grapevine: a step towards genetic improvement.

    abstract::In the past few years, the usefulness of transient expression assays has continuously increased for the characterization of unknown gene function and metabolic pathways. In grapevine (Vitis vinifera L.), one of the most economically important fruit crops in the world, recent systematic sequencing projects produced man...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/pbi.12294

    authors: Jelly NS,Valat L,Walter B,Maillot P

    更新日期:2014-12-01 00:00:00

  • Induction and recovery of copy number variation in banana through gamma irradiation and low-coverage whole-genome sequencing.

    abstract::Traditional breeding methods are hindered in bananas due to the fact that major cultivars are sterile, parthenocarpic, triploid and thus clonally propagated. This has resulted in a narrow genetic base and limited resilience to biotic and abiotic stresses. Mutagenesis of in vitro propagated bananas is one method to int...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12901

    authors: Datta S,Jankowicz-Cieslak J,Nielen S,Ingelbrecht I,Till BJ

    更新日期:2018-02-24 00:00:00

  • Cytokinin-mediated source/sink modifications improve drought tolerance and increase grain yield in rice under water-stress.

    abstract::Drought is the major environmental factor limiting crop productivity worldwide. We hypothesized that it is possible to enhance drought tolerance by delaying stress-induced senescence through the stress-induced synthesis of cytokinins in crop-plants. We generated transgenic rice (Oryza sativa) plants expressing an isop...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2010.00584.x

    authors: Peleg Z,Reguera M,Tumimbang E,Walia H,Blumwald E

    更新日期:2011-09-01 00:00:00

  • Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase.

    abstract::Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer c...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12359

    authors: Ramírez-Estrada K,Altabella T,Onrubia M,Moyano E,Notredame C,Osuna L,Vanden Bossche R,Goossens A,Cusido RM,Palazon J

    更新日期:2016-01-01 00:00:00

  • Foreign gene products can be enhanced by introduction into low storage protein mutants.

    abstract::Transgenic rice expressing soybean glycinin in its endosperm was crossed with two types of low-glutelin mutants to determine how much storage the protein mutants can contribute to increases in glycinin accumulation. The glycinin level (102 microg/100 mg seed) in the parental transgenic line was enhanced to approximate...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1046/j.1467-7652.2003.00038.x

    authors: Tada Y,Utsumi S,Takaiwa F

    更新日期:2003-11-01 00:00:00

  • Induction, rapid fixation and retention of mutations in vegetatively propagated banana.

    abstract::Mutation discovery technologies have enabled the development of reverse genetics for many plant species and allowed sophisticated evaluation of the consequences of mutagenesis. Such methods are relatively straightforward for seed-propagated plants. To develop a platform suitable for vegetatively propagated species, we...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2012.00733.x

    authors: Jankowicz-Cieslak J,Huynh OA,Brozynska M,Nakitandwe J,Till BJ

    更新日期:2012-12-01 00:00:00

  • Biofortification of plants with altered antioxidant content and composition: genetic engineering strategies.

    abstract::Antioxidants are protective molecules that neutralize reactive oxygen species and prevent oxidative damage to cellular components such as membranes, proteins and nucleic acids, therefore reducing the rate of cell death and hence the effects of ageing and ageing-related diseases. The fortification of food with antioxid...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/j.1467-7652.2012.00740.x

    authors: Zhu C,Sanahuja G,Yuan D,Farré G,Arjó G,Berman J,Zorrilla-López U,Banakar R,Bai C,Pérez-Massot E,Bassie L,Capell T,Christou P

    更新日期:2013-02-01 00:00:00

  • Engineering of enhanced glycine betaine synthesis improves drought tolerance in maize.

    abstract::Glycine betaine plays an important role in some plants, including maize, in conditions of abiotic stress, but different maize varieties vary in their capacity to accumulate glycine betaine. An elite maize inbred line DH4866 was transformed with the betA gene from Escherichia coli encoding choline dehydrogenase (EC 1.1...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2004.00093.x

    authors: Quan R,Shang M,Zhang H,Zhao Y,Zhang J

    更新日期:2004-11-01 00:00:00

  • Engineering rice with lower grain arsenic.

    abstract::Arsenic (As) is a poisonous element that causes severe skin lesions and cancer in humans. Rice (Oryza sativa L.) is a major dietary source of As in humans who consume this cereal as a staple food. We hypothesized that increasing As vacuolar sequestration would inhibit its translocation into the grain and reduce the am...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12905

    authors: Deng F,Yamaji N,Ma JF,Lee SK,Jeon JS,Martinoia E,Lee Y,Song WY

    更新日期:2018-10-01 00:00:00

  • De novo assembly of a wild pear (Pyrus betuleafolia) genome.

    abstract::China is the origin and evolutionary centre of Oriental pears. Pyrus betuleafolia is a wild species native to China and distributed in the northern region, and it is widely used as rootstock. Here, we report the de novo assembly of the genome of P. betuleafolia-Shanxi Duli using an integrated strategy that combines Pa...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13226

    authors: Dong X,Wang Z,Tian L,Zhang Y,Qi D,Huo H,Xu J,Li Z,Liao R,Shi M,Wahocho SA,Liu C,Zhang S,Tian Z,Cao Y

    更新日期:2020-02-01 00:00:00

  • Enhancement of essential amino acid contents in crops by genetic engineering and protein design.

    abstract::The importance and urgency of providing humans and animals with quality proteins are reflected in the growing scientific and industrial interest in augmenting the nutritive value of the world's protein sources. Such nutritive value is determined by the protein content in 'essential amino acids', those that cannot be s...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/j.1467-7652.2006.00204.x

    authors: Beauregard M,Hefford MA

    更新日期:2006-09-01 00:00:00

  • Down-regulated expression of plant-specific glycoepitopes in alfalfa.

    abstract:SUMMARY:Compared with other plant expression systems used for pharmaceutical protein production, alfalfa offers the advantage of very homogeneous N-glycosylation. Therefore, this plant was selected for further attempts at glycoengineering. Two main approaches were developed in order to humanize N-glycosylation in alfal...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2008.00353.x

    authors: Sourrouille C,Marquet-Blouin E,D'Aoust MA,Kiefer-Meyer MC,Seveno M,Pagny-Salehabadi S,Bardor M,Durambur G,Lerouge P,Vezina L,Gomord V

    更新日期:2008-09-01 00:00:00

  • A maize phytochrome-interacting factors protein ZmPIF1 enhances drought tolerance by inducing stomatal closure and improves grain yield in Oryza sativa.

    abstract::Phytochrome-interacting factors (PIFs) play major roles in regulating plant growth and development, but their roles in drought stress remain elusive. Here, we cloned and characterized a maize (Zea mays) PIF transcription factor, ZmPIF1. The expression level of ZmPIF1 was significantly induced by independent drought an...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12878

    authors: Gao Y,Wu M,Zhang M,Jiang W,Ren X,Liang E,Zhang D,Zhang C,Xiao N,Li Y,Dai Y,Chen J

    更新日期:2018-07-01 00:00:00

  • The impact of secondary pests on Bacillus thuringiensis (Bt) crops.

    abstract::The intensification of agriculture and the development of synthetic insecticides enabled worldwide grain production to more than double in the last third of the 20th century. However, the heavy dependence and, in some cases, overuse of insecticides has been responsible for negative environmental and ecological impacts...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/pbi.12363

    authors: Catarino R,Ceddia G,Areal FJ,Park J

    更新日期:2015-06-01 00:00:00

  • Recombinant aryl hydrocarbon receptors for bioassay of aryl hydrocarbon receptor ligands in transgenic tobacco plants.

    abstract::Dioxin residues widely contaminate soil and agricultural products at low concentrations and may accumulate in organisms at the top of food chains owing to their physicochemical properties. In this study, we have developed novel, dioxin-inducible, reporter gene expression systems regulated by recombinant aryl hydrocarb...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2008.00378.x

    authors: Kodama S,Okada K,Akimoto K,Inui H,Ohkawa H

    更新日期:2009-02-01 00:00:00

  • Genome-wide association study discovered genetic variation and candidate genes of fibre quality traits in Gossypium hirsutum L.

    abstract::Genetic improvement of fibre quality is one of the main breeding goals for the upland cotton, Gossypium hirsutum, but there are difficulties with precise selection of traits. Therefore, it is important to improve the understanding of the genetic basis of phenotypic variation. In this study, we conducted phenotyping an...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12693

    authors: Sun Z,Wang X,Liu Z,Gu Q,Zhang Y,Li Z,Ke H,Yang J,Wu J,Wu L,Zhang G,Zhang C,Ma Z

    更新日期:2017-08-01 00:00:00

  • Regulating innovative crop technologies in Canada: the case of regulating genetically modified crops.

    abstract::The advent of genetically modified crops in the late 1980s triggered a regulatory response to the relatively new field of plant genetic engineering. Over a 7-year period, a new regulatory framework was created, based on scientific principles that focused on risk mitigation. The process was transparent and deliberately...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/j.1467-7652.2007.00309.x

    authors: Smyth S,McHughen A

    更新日期:2008-04-01 00:00:00