Engineering bacteriocin-mediated resistance against the plant pathogen Pseudomonas syringae.

abstract：:The importance and urgency of providing humans and animals with quality proteins are reflected in the growing scientific and industrial interest in augmenting the nutritive value of the world's protein sources. Such nutritive value is determined by the protein content in 'essential amino acids', those that cannot be s...

journal_title：Plant biotechnology journal

authors： Beauregard M,Hefford MA

更新日期：2006-09-01 00:00:00

abstract：:Approximately 20,000 of the rice-FOX Arabidopsis transgenic lines, which overexpress 13,000 rice full-length cDNAs at random in Arabidopsis, were screened for bacterial disease resistance by dip inoculation with Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). The identities of the overexpressed genes were determi...

journal_title：Plant biotechnology journal

authors： Dubouzet JG,Maeda S,Sugano S,Ohtake M,Hayashi N,Ichikawa T,Kondou Y,Kuroda H,Horii Y,Matsui M,Oda K,Hirochika H,Takatsuji H,Mori M

更新日期：2011-05-01 00:00:00

abstract：:We describe the site-directed integration (SDI) system for Agrobacterium-mediated transformation to precisely integrate a single copy of a desired gene into a predefined target locus by recombinase-mediated cassette exchange (RMCE). The system requires the selection of a transformed line with an integrated copy of a t...

journal_title：Plant biotechnology journal

authors： Nanto K,Yamada-Watanabe K,Ebinuma H

更新日期：2005-03-01 00:00:00

abstract：:Genetic modification of Zn/Cd accumulation in roots and shoots for biofortification or phytoremediation is a focus of this manuscript. We expressed AtHMA4 (a P(₁B) ATPase involved in Zn and Cd transport), AtHMA4-trunc (lacking the C-terminal region) and AtHMA4-C terminus (the C-terminal region alone) in tobacco under ...

journal_title：Plant biotechnology journal

authors： Siemianowski O,Mills RF,Williams LE,Antosiewicz DM

更新日期：2011-01-01 00:00:00

abstract：:Heavy metal accumulation in the environment poses great risks to flora and fauna. However, monitoring sites prone to accumulation poses scale and economic challenges. In this study, we present and test a method for monitoring these sites using fluorescent resonance energy transfer (FRET) change in response to zinc (Zn...

journal_title：Plant biotechnology journal

authors： Adams JP,Adeli A,Hsu CY,Harkess RL,Page GP,Depamphilis CW,Schultz EB,Yuceer C

更新日期：2012-02-01 00:00:00

abstract：:Genetic engineering to enhance mercury phytoremediation has been accomplished by expression of the merAB genes that protects the cell by converting Hg[II] into Hg[0] which volatilizes from the cell. A drawback of this approach is that toxic Hg is released back into the environment. A better phytoremediation strategy w...

journal_title：Plant biotechnology journal

authors： Ruiz ON,Alvarez D,Torres C,Roman L,Daniell H

更新日期：2011-06-01 00:00:00

abstract：:Despite progress in the prevention and treatment of infectious diseases, they continue to present a major threat to public health. The frequency of emerging and reemerging infections and the risk of bioterrorism warrant significant efforts towards the development of prophylactic and therapeutic countermeasures. Vaccin...

journal_title：Plant biotechnology journal

authors： Streatfield SJ,Kushnir N,Yusibov V

更新日期：2015-10-01 00:00:00

abstract：:Pollen germination and pollen tube growth are important physiological processes of sexual reproduction of plants and also are involved in signal transduction. Our previous study reveals that ZmSTK1 and ZmSTK2 are two receptor-like cytoplasmic kinases (RLCK) homologs in Zea mays as members of receptor-like protein kina...

journal_title：Plant biotechnology journal

authors： Fan M,Zhang C,Shi L,Liu C,Ma W,Chen M,Liu K,Cai F,Wang G,Wei Z,Jiang M,Liu Z,Javeed A,Lin F

更新日期：2018-08-01 00:00:00

abstract：:Multivitamin corn is a novel genetically engineered variety that simultaneously produces high levels of β-carotene, ascorbate and folate, and therefore has the potential to address simultaneously multiple micronutrient deficiencies caused by the lack of vitamins A, B9 and C in developing country populations. As part o...

journal_title：Plant biotechnology journal

authors： Arjó G,Capell T,Matias-Guiu X,Zhu C,Christou P,Piñol C

更新日期：2012-12-01 00:00:00

abstract：:Protein microbicides containing neutralizing antibodies and antiviral lectins may help to reduce the rate of infection with human immunodeficiency virus (HIV) if it is possible to manufacture the components in large quantities at a cost affordable in HIV-endemic regions such as sub-Saharan Africa. We expressed the ant...

journal_title：Plant biotechnology journal

authors： Vamvaka E,Arcalis E,Ramessar K,Evans A,O'Keefe BR,Shattock RJ,Medina V,Stöger E,Christou P,Capell T

更新日期：2016-06-01 00:00:00

abstract：:Spike lavender (Lavandula latifolia) essential oil is widely used in the perfume, cosmetic, flavouring and pharmaceutical industries. Thus, modifications of yield and composition of this essential oil by genetic engineering should have important scientific and commercial applications. We generated transgenic spike lav...

journal_title：Plant biotechnology journal

authors： Muñoz-Bertomeu J,Sales E,Ros R,Arrillaga I,Segura J

更新日期：2007-11-01 00:00:00

abstract：:The identification of genetic markers linked to genes of agronomic importance is a major aim of crop research and breeding programmes. Here, we identify markers for Yr15, a major disease resistance gene for wheat yellow rust, using a segregating F2 population. After phenotyping, we implemented RNA sequencing (RNA-Seq)...

journal_title：Plant biotechnology journal

authors： Ramirez-Gonzalez RH,Segovia V,Bird N,Fenwick P,Holdgate S,Berry S,Jack P,Caccamo M,Uauy C

更新日期：2015-06-01 00:00:00

abstract：:Anthocyanins have crucial biological functions and affect quality of horticultural produce. Anthocyanins accumulate in ripe peach fruit; differential accumulation is observed in deep coloured cultivar 'Hujingmilu' and lightly pigmented cultivar 'Yulu'. The difference was not fully explained by accumulation of total fl...

journal_title：Plant biotechnology journal

authors： Zhao Y,Dong W,Zhu Y,Allan AC,Lin-Wang K,Xu C

更新日期：2020-05-01 00:00:00

abstract：:Methods based on single nucleotide polymorphism (SNP), copy number variation (CNV) and presence/absence variation (PAV) discovery provide a valuable resource to study gene structure and evolution. However, as a result of these structural variations, a single reference genome is unable to cover the entire gene content ...

journal_title：Plant biotechnology journal

authors： Dolatabadian A,Bayer PE,Tirnaz S,Hurgobin B,Edwards D,Batley J

更新日期：2020-04-01 00:00:00

abstract：:Tomato (Solanum lycopersicum) fruit ripening is regulated co-operatively by the action of ethylene and a hierarchy of transcription factors, including RIPENING INHIBITOR (RIN) and NON-RIPENING (NOR). Mutations in these two genes have been adopted commercially to delay ripening, and accompanying textural deterioration,...

journal_title：Plant biotechnology journal

authors： Osorio S,Carneiro RT,Lytovchenko A,McQuinn R,Sørensen I,Vallarino JG,Giovannoni JJ,Fernie AR,Rose JKC

更新日期：2020-01-01 00:00:00

abstract：:The cooking quality of rice is associated with the starch gelatinization temperature (GT). Rice genotypes with low GT have probably been selected for their cooking quality by humans during domestication. We now report polymorphisms in starch synthase IIa (SSIIa) that explain the variation in rice starch GT. Sequence a...

journal_title：Plant biotechnology journal

authors： Waters DL,Henry RJ,Reinke RF,Fitzgerald MA

更新日期：2006-01-01 00:00:00

abstract：:Sumoylation is a posttranslational regulatory process in higher eukaryotes modifying substrate proteins through conjugation of small ubiquitin-related modifiers (SUMOs). Sumoylation modulates protein stability, subcellular localization and activity; thus, it regulates most cellular functions including response to envi...

journal_title：Plant biotechnology journal

authors： Li Z,Hu Q,Zhou M,Vandenbrink J,Li D,Menchyk N,Reighard S,Norris A,Liu H,Sun D,Luo H

更新日期：2013-05-01 00:00:00

abstract：:Transgenic rice accumulating the modified major Japanese cedar pollen allergens, Cryptomeria japonica 1 (Cry j 1) and Cryptomeria japonica 2 (Cry j 2), which were deconstructed by fragmentation and shuffling, respectively, in the edible part of the seed was generated by transformation of a good-tasting rice variety, '...

journal_title：Plant biotechnology journal

authors： Wakasa Y,Takagi H,Hirose S,Yang L,Saeki M,Nishimura T,Kaminuma O,Hiroi T,Takaiwa F

更新日期：2013-01-01 00:00:00

abstract：:Genetic engineering with just a few genes has changed agriculture in the last 20 years. The most frequently used transgenes are the herbicide resistance genes for efficient weed control and the Bt toxin genes for insect resistance. The adoption of the first-generation genetically engineered crops has been very success...

journal_title：Plant biotechnology journal

authors： Yu W,Yau YY,Birchler JA

更新日期：2016-05-01 00:00:00

abstract：:Drought conditions are among the most serious challenges to crop production worldwide. Here, we report the results of field evaluations of transgenic rice plants overexpressing OsNAC5, under the control of either the root-specific (RCc3) or constitutive (GOS2) promoters. Field evaluations over three growing seasons re...

journal_title：Plant biotechnology journal

authors： Jeong JS,Kim YS,Redillas MC,Jang G,Jung H,Bang SW,Choi YD,Ha SH,Reuzeau C,Kim JK

更新日期：2013-01-01 00:00:00

abstract：:There is an urgent need to provide effective anti-HIV microbicides to resource-poor areas worldwide. Some of the most promising microbicide candidates are biotherapeutics targeting viral entry. To provide biotherapeutics to poorer areas, it is vital to reduce the cost. Here, we report the production of biologically ac...

journal_title：Plant biotechnology journal

authors： O'Keefe BR,Murad AM,Vianna GR,Ramessar K,Saucedo CJ,Wilson J,Buckheit KW,da Cunha NB,Araújo AC,Lacorte CC,Madeira L,McMahon JB,Rech EL

更新日期：2015-09-01 00:00:00

abstract：:Plant genetic engineering can create transgenic crops with improved characteristics by introducing trait genes through transformation. Appropriate regulatory elements such as promoters and terminators have to be present in certain configurations for the transgenes to be properly expressed. Five terminators native to s...

journal_title：Plant biotechnology journal

authors： Xing A,Moon BP,Mills KM,Falco SC,Li Z

更新日期：2010-09-01 00:00:00

abstract：:Steviol glycosides (SGs) are extracted from Stevia leaves for use as a natural sweetener. Among SGs, stevioside is most abundant in leaf extracts followed by rebaudioside A (Reb A). However, Reb A is of particular interest because of its sweeter and more pleasant taste compared to stevioside. Therefore, the developmen...

journal_title：Plant biotechnology journal

authors： Kim MJ,Zheng J,Liao MH,Jang IC

更新日期：2019-06-01 00:00:00

abstract：:Next-generation sequencing of complete genomes has given researchers unprecedented levels of information to study the multifaceted evolutionary changes that have shaped elite plant germplasm. In conjunction with population genetic analytical techniques and detailed online databases, we can more accurately capture the ...

journal_title：Plant biotechnology journal

authors： Campbell BC,Gilding EK,Mace ES,Tai S,Tao Y,Prentis PJ,Thomelin P,Jordan DR,Godwin ID

更新日期：2016-12-01 00:00:00

abstract：:The rapid accumulation of defensive transgene products in plants only on pathogen invasion has clear advantages over their constitutive synthesis. In this study, two antimicrobial peptides from the skin secretions of frogs, MsrA2 (N-methionine-dermaseptin B1) and temporin A, were evaluated for engineering pathogen-ind...

journal_title：Plant biotechnology journal

authors： Yevtushenko DP,Misra S

更新日期：2007-11-01 00:00:00

abstract：:Transgenic engineering of plants is important in both basic and applied research. However, the expression of a transgene can dwindle over time as the plant's small (s)RNA-guided silencing pathways shut it down. The silencing pathways have evolved as antiviral defence mechanisms, and viruses have co-evolved viral silen...

journal_title：Plant biotechnology journal

authors： Naim F,Shrestha P,Singh SP,Waterhouse PM,Wood CC

更新日期：2016-06-01 00:00:00

abstract：:Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is severely damaging to the global citrus industry. Targeted editing of host disease-susceptibility genes represents an interesting and potentially durable alternative in plant breeding for resistance. Here, we report improvement of citrus canker resistanc...

journal_title：Plant biotechnology journal

authors： Peng A,Chen S,Lei T,Xu L,He Y,Wu L,Yao L,Zou X

更新日期：2017-12-01 00:00:00

abstract：:The early methionine (Em) proteins are members of the late embryogenesis abundant (LEA) group of proteins that have been considered to be embryo specific. The ability of a 646-bp wheat Em promoter to control green fluorescent protein (gfp) expression was investigated in transgenic barley and rice. Seeds of transgenic ...

journal_title：Plant biotechnology journal

authors： Furtado A,Henry RJ

更新日期：2005-07-01 00:00:00

abstract：:DNA recombination reactions (site-specific and homologous) were monitored in the progeny of transgenic maize plants by bringing together two recombination substrates (docking sites and shuttle vectors) in the zygotes. In one combination of transgenic events, the recombination marker gene (yellow fluorescent protein ge...

journal_title：Plant biotechnology journal

authors： Djukanovic V,Orczyk W,Gao H,Sun X,Garrett N,Zhen S,Gordon-Kamm W,Barton J,Lyznik LA

更新日期：2006-05-01 00:00:00

abstract：:Although much explored, oral tolerance for treatment of autoimmune diseases still awaits the establishment of novel and effective vectors. We investigated whether the tolerogenic CTA1(R7K)-COL-DD fusion protein can be expressed in edible plants, to induce oral tolerance and protect against arthritis. The fusion protei...

journal_title：Plant biotechnology journal

authors： Hansson C,Schön K,Kalbina I,Strid Å,Andersson S,Bokarewa MI,Lycke NY

更新日期：2016-04-01 00:00:00