Engineering soya bean seeds as a scalable platform to produce cyanovirin-N, a non-ARV microbicide against HIV.

abstract：:S-adenosyl-L-methionine (SAM) synthetase is the key enzyme involved in the biosynthesis of SAM, which serves as a common precursor for polyamines (PAs) and ethylene. A SAM synthetase cDNA (SlSAMS1) was introduced into the tomato genome using the Agrobacterium tumefaciens transformation method. Transgenic plants overex...

journal_title：Plant biotechnology journal

authors： Gong B,Li X,VandenLangenberg KM,Wen D,Sun S,Wei M,Li Y,Yang F,Shi Q,Wang X

更新日期：2014-08-01 00:00:00

abstract：:Drought tolerance of the wheat cultivar Bobwhite was previously enhanced by transformation with a construct containing the wheat DREB3 gene driven by the stress-inducible maize Rab17 promoter. Progeny of a single T2 transgenic line were used as pollinators in crosses with four elite bread wheat cultivars from Western ...

journal_title：Plant biotechnology journal

authors： Shavrukov Y,Baho M,Lopato S,Langridge P

更新日期：2016-01-01 00:00:00

abstract：:Extracellular plant peptides perform a large variety of functions, including signalling and defence. Intracellular peptides often have physiological functions or may merely be the products of general proteolysis. Plant peptides have been identified and, in part, functionally characterized through biochemical and genet...

journal_title：Plant biotechnology journal

authors： Farrokhi N,Whitelegge JP,Brusslan JA

更新日期：2008-02-01 00:00:00

abstract：:The plant immune system involves detection of pathogens via both cell-surface and intracellular receptors. Both receptor classes can induce transcriptional reprogramming that elevates disease resistance. To assess differential gene expression during plant immunity, we developed and deployed quantitative sequence captu...

journal_title：Plant biotechnology journal

authors： Ding P,Ngou BPM,Furzer OJ,Sakai T,Shrestha RK,MacLean D,Jones JDG

更新日期：2020-07-01 00:00:00

abstract：:Low grain moisture at harvest is crucial for safe production, transport, and storage, but the genetic architecture of this trait in maize (Zea mays) remains elusive. Here, we measured the dynamic changes in grain moisture content in an association mapping panel of 513 diverse maize inbred lines at five successive stag...

journal_title：Plant biotechnology journal

authors： Li W,Yu Y,Wang L,Luo Y,Peng Y,Xu Y,Liu X,Wu S,Jian L,Xu J,Xiao Y,Yan J

更新日期：2021-01-01 00:00:00

abstract：:For future food security, it is important that wheat, one of the most widely consumed crops in the world, can survive the threat of abiotic and biotic stresses. New genetic variation is currently being introduced into wheat through introgressions from its wild relatives. For trait discovery, it is necessary that each ...

journal_title：Plant biotechnology journal

authors： Grewal S,Hubbart-Edwards S,Yang C,Devi U,Baker L,Heath J,Ashling S,Scholefield D,Howells C,Yarde J,Isaac P,King IP,King J

更新日期：2020-03-01 00:00:00

abstract：:Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer c...

journal_title：Plant biotechnology journal

authors： Ramírez-Estrada K,Altabella T,Onrubia M,Moyano E,Notredame C,Osuna L,Vanden Bossche R,Goossens A,Cusido RM,Palazon J

更新日期：2016-01-01 00:00:00

abstract：:Expression of the cecropin A gene in rice confers resistance to the rice blast fungus Magnaporthe oryzae. In this study, a polymerase chain reaction-based suppression subtractive hybridization approach was used to generate a cDNA macroarray from the elite japonica rice (Oryza sativa L.) cultivar 'Senia'. Gene expressi...

journal_title：Plant biotechnology journal

authors： Campo S,Manrique S,García-Martínez J,San Segundo B

更新日期：2008-08-01 00:00:00

abstract：:Virus-induced gene silencing (VIGS) is a powerful tool for functional analysis of genes in plants. A wide-host-range VIGS vector, which was developed based on the Cucumber mosaic virus (CMV), was tested for its ability to silence endogenous genes involved in flavonoid biosynthesis in soybean. Symptomless infection was...

journal_title：Plant biotechnology journal

authors： Nagamatsu A,Masuta C,Senda M,Matsuura H,Kasai A,Hong JS,Kitamura K,Abe J,Kanazawa A

更新日期：2007-11-01 00:00:00

abstract：:Approximately 20,000 of the rice-FOX Arabidopsis transgenic lines, which overexpress 13,000 rice full-length cDNAs at random in Arabidopsis, were screened for bacterial disease resistance by dip inoculation with Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). The identities of the overexpressed genes were determi...

journal_title：Plant biotechnology journal

authors： Dubouzet JG,Maeda S,Sugano S,Ohtake M,Hayashi N,Ichikawa T,Kondou Y,Kuroda H,Horii Y,Matsui M,Oda K,Hirochika H,Takatsuji H,Mori M

更新日期：2011-05-01 00:00:00

abstract：:GM crops are the most studied crops in history. Approximately 5% of the safety studies on them show adverse effects that are a cause for concern and tend to be featured in media reports. Although these reports are based on just a handful of GM events, they are used to cast doubt on all GM crops. Furthermore, they tend...

journal_title：Plant biotechnology journal

authors： Sánchez MA,Parrott WA

更新日期：2017-10-01 00:00:00

abstract：:Antioxidants are protective molecules that neutralize reactive oxygen species and prevent oxidative damage to cellular components such as membranes, proteins and nucleic acids, therefore reducing the rate of cell death and hence the effects of ageing and ageing-related diseases. The fortification of food with antioxid...

journal_title：Plant biotechnology journal

authors： Zhu C,Sanahuja G,Yuan D,Farré G,Arjó G,Berman J,Zorrilla-López U,Banakar R,Bai C,Pérez-Massot E,Bassie L,Capell T,Christou P

更新日期：2013-02-01 00:00:00

abstract：:Pollen germination and pollen tube growth are important physiological processes of sexual reproduction of plants and also are involved in signal transduction. Our previous study reveals that ZmSTK1 and ZmSTK2 are two receptor-like cytoplasmic kinases (RLCK) homologs in Zea mays as members of receptor-like protein kina...

journal_title：Plant biotechnology journal

authors： Fan M,Zhang C,Shi L,Liu C,Ma W,Chen M,Liu K,Cai F,Wang G,Wei Z,Jiang M,Liu Z,Javeed A,Lin F

更新日期：2018-08-01 00:00:00

abstract：:Genetic improvement of fibre quality is one of the main breeding goals for the upland cotton, Gossypium hirsutum, but there are difficulties with precise selection of traits. Therefore, it is important to improve the understanding of the genetic basis of phenotypic variation. In this study, we conducted phenotyping an...

journal_title：Plant biotechnology journal

authors： Sun Z,Wang X,Liu Z,Gu Q,Zhang Y,Li Z,Ke H,Yang J,Wu J,Wu L,Zhang G,Zhang C,Ma Z

更新日期：2017-08-01 00:00:00

abstract：:Heavy metal accumulation in the environment poses great risks to flora and fauna. However, monitoring sites prone to accumulation poses scale and economic challenges. In this study, we present and test a method for monitoring these sites using fluorescent resonance energy transfer (FRET) change in response to zinc (Zn...

journal_title：Plant biotechnology journal

authors： Adams JP,Adeli A,Hsu CY,Harkess RL,Page GP,Depamphilis CW,Schultz EB,Yuceer C

更新日期：2012-02-01 00:00:00

abstract：:Salicylic acid (SA) is an essential hormone for plant defence and development. SA perception is usually measured by counting the number of pathogens that grow in planta upon an exogenous application of the hormone. A biological SA perception model based on plant fresh weight reduction caused by disease resistance in A...

journal_title：Plant biotechnology journal

authors： Canet JV,Dobón A,Ibáñez F,Perales L,Tornero P

更新日期：2010-02-01 00:00:00

abstract：:To infect plants, viruses rely heavily on their host's machinery. Plant genetic resistances based on host factor modifications can be found among existing natural variability and are widely used for some but not all crops. While biotechnology can supply for the lack of natural resistance alleles, new strategies need t...

journal_title：Plant biotechnology journal

authors： Bastet A,Lederer B,Giovinazzo N,Arnoux X,German-Retana S,Reinbold C,Brault V,Garcia D,Djennane S,Gersch S,Lemaire O,Robaglia C,Gallois JL

更新日期：2018-03-05 00:00:00

abstract：:Following the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing (dRenSeq) enables the high-confidence i...

journal_title：Plant biotechnology journal

authors： Armstrong MR,Vossen J,Lim TY,Hutten RCB,Xu J,Strachan SM,Harrower B,Champouret N,Gilroy EM,Hein I

更新日期：2019-02-01 00:00:00

abstract：:The rapid accumulation of defensive transgene products in plants only on pathogen invasion has clear advantages over their constitutive synthesis. In this study, two antimicrobial peptides from the skin secretions of frogs, MsrA2 (N-methionine-dermaseptin B1) and temporin A, were evaluated for engineering pathogen-ind...

journal_title：Plant biotechnology journal

authors： Yevtushenko DP,Misra S

更新日期：2007-11-01 00:00:00

abstract：:Dioxin residues widely contaminate soil and agricultural products at low concentrations and may accumulate in organisms at the top of food chains owing to their physicochemical properties. In this study, we have developed novel, dioxin-inducible, reporter gene expression systems regulated by recombinant aryl hydrocarb...

journal_title：Plant biotechnology journal

authors： Kodama S,Okada K,Akimoto K,Inui H,Ohkawa H

更新日期：2009-02-01 00:00:00

abstract：:Unintended gene flow from transgenic plants via pollen, seed and vegetative propagation is a regulatory concern because of potential admixture in food and crop systems, as well as hybridization and introgression to wild and weedy relatives. Bioconfinement of transgenic pollen would help address some of these concerns ...

journal_title：Plant biotechnology journal

authors： Millwood RJ,Moon HS,Poovaiah CR,Muthukumar B,Rice JH,Abercrombie JM,Abercrombie LL,Green WD,Stewart CN Jr

更新日期：2016-05-01 00:00:00

abstract：:Drought conditions limit agricultural production by preventing crops from reaching their genetically predetermined maximum yields. Here, we present the results of field evaluations of rice overexpressing OsNAC9, a member of the rice NAC domain family. Root-specific (RCc3) and constitutive (GOS2) promoters were used to...

journal_title：Plant biotechnology journal

authors： Redillas MC,Jeong JS,Kim YS,Jung H,Bang SW,Choi YD,Ha SH,Reuzeau C,Kim JK

更新日期：2012-09-01 00:00:00

abstract：:In the past few years, the usefulness of transient expression assays has continuously increased for the characterization of unknown gene function and metabolic pathways. In grapevine (Vitis vinifera L.), one of the most economically important fruit crops in the world, recent systematic sequencing projects produced man...

journal_title：Plant biotechnology journal

authors： Jelly NS,Valat L,Walter B,Maillot P

更新日期：2014-12-01 00:00:00

abstract：:The related A genome species of the Oryza genus are the effective gene pool for rice. Here, we report draft genomes for two Australian wild A genome taxa: O. rufipogon-like population, referred to as Taxon A, and O. meridionalis-like population, referred to as Taxon B. These two taxa were sequenced and assembled by in...

journal_title：Plant biotechnology journal

authors： Brozynska M,Copetti D,Furtado A,Wing RA,Crayn D,Fox G,Ishikawa R,Henry RJ

更新日期：2017-06-01 00:00:00

abstract：:Squalene is a linear intermediate to nearly all classes of triterpenes and sterols and is itself highly valued for its use in wide range of industrial applications. Another unique linear triterpene is botryococcene and its methylated derivatives generated by the alga Botryococcus braunii race B, which are progenitors ...

journal_title：Plant biotechnology journal

authors： Jiang Z,Kempinski C,Kumar S,Kinison S,Linscott K,Nybo E,Janze S,Wood C,Chappell J

更新日期：2018-06-01 00:00:00

abstract：:Differences in gene sequences, many of which are single nucleotide polymorphisms, underlie some of the most important traits in plants. With humanity facing significant challenges to increase global agricultural productivity, there is an urgent need to accelerate the development of these traits in plants. oligonucleot...

journal_title：Plant biotechnology journal

authors： Sauer NJ,Mozoruk J,Miller RB,Warburg ZJ,Walker KA,Beetham PR,Schöpke CR,Gocal GF

更新日期：2016-02-01 00:00:00

abstract：:The cooking quality of rice is associated with the starch gelatinization temperature (GT). Rice genotypes with low GT have probably been selected for their cooking quality by humans during domestication. We now report polymorphisms in starch synthase IIa (SSIIa) that explain the variation in rice starch GT. Sequence a...

journal_title：Plant biotechnology journal

authors： Waters DL,Henry RJ,Reinke RF,Fitzgerald MA

更新日期：2006-01-01 00:00:00

abstract：:Although much explored, oral tolerance for treatment of autoimmune diseases still awaits the establishment of novel and effective vectors. We investigated whether the tolerogenic CTA1(R7K)-COL-DD fusion protein can be expressed in edible plants, to induce oral tolerance and protect against arthritis. The fusion protei...

journal_title：Plant biotechnology journal

authors： Hansson C,Schön K,Kalbina I,Strid Å,Andersson S,Bokarewa MI,Lycke NY

更新日期：2016-04-01 00:00:00

abstract：:In this article, we explore the intellectual property (IP) landscape relevant to the production and commercialization of Carolight(™) , a transgenic multivitamin corn variety created on humanitarian grounds to address micronutrient deficiencies in low-and-middle-income countries. The successful production of this vari...

journal_title：Plant biotechnology journal

authors： Zanga D,Capell T,Zhu C,Christou P,Thangaraj H

更新日期：2016-05-01 00:00:00

abstract：:Ascorbate is a powerful antioxidant in plants, and its levels are an important quality criteria in commercial species. Factors influencing these levels include environmental variations, particularly light, and the genetic control of its biosynthesis, recycling and degradation. One of the genes involved in the recyclin...

journal_title：Plant biotechnology journal

authors： Gest N,Garchery C,Gautier H,Jiménez A,Stevens R

更新日期：2013-04-01 00:00:00