Engineered selective plant male sterility through pollen-specific expression of the EcoRI restriction endonuclease.

Abstract:

:Unintended gene flow from transgenic plants via pollen, seed and vegetative propagation is a regulatory concern because of potential admixture in food and crop systems, as well as hybridization and introgression to wild and weedy relatives. Bioconfinement of transgenic pollen would help address some of these concerns and enable transgenic plant production for several crops where gene flow is an issue. Here, we demonstrate the expression of the restriction endonuclease EcoRI under the control of the tomato pollen-specific LAT52 promoter is an effective method for generating selective male sterility in Nicotiana tabacum (tobacco). Of nine transgenic events recovered, four events had very high bioconfinement with tightly controlled EcoRI expression in pollen and negligible-to-no expression other plant tissues. Transgenic plants had normal morphology wherein vegetative growth and reproductivity were similar to nontransgenic controls. In glasshouse experiments, transgenic lines were hand-crossed to both male-sterile and emasculated nontransgenic tobacco varieties. Progeny analysis of 16 000-40 000 seeds per transgenic line demonstrated five lines approached (>99.7%) or attained 100% bioconfinement for one or more generations. Bioconfinement was again demonstrated at or near 100% under field conditions where four transgenic lines were grown in close proximity to male-sterile tobacco, and 900-2100 seeds per male-sterile line were analysed for transgenes. Based upon these results, we conclude EcoRI-driven selective male sterility holds practical potential as a safe and reliable transgene bioconfinement strategy. Given the mechanism of male sterility, this method could be applicable to any plant species.

journal_name

Plant Biotechnol J

authors

Millwood RJ,Moon HS,Poovaiah CR,Muthukumar B,Rice JH,Abercrombie JM,Abercrombie LL,Green WD,Stewart CN Jr

doi

10.1111/pbi.12493

subject

Has Abstract

pub_date

2016-05-01 00:00:00

pages

1281-90

issue

5

eissn

1467-7644

issn

1467-7652

journal_volume

14

pub_type

杂志文章
  • Genetic modification of cassava for enhanced starch production.

    abstract::To date, transgenic approaches to biofortify subsistence crops have been rather limited. This is particularly true for the starchy root crop cassava (Manihot esculenta Crantz). Cassava has one of the highest rates of CO(2) fixation and sucrose synthesis for any C3 plant, but rarely reaches its yield potentials in the ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2006.00195.x

    authors: Ihemere U,Arias-Garzon D,Lawrence S,Sayre R

    更新日期:2006-07-01 00:00:00

  • Production of recombinant human granulocyte macrophage-colony stimulating factor in rice cell suspension culture with a human-like N-glycan structure.

    abstract::The rice α-amylase 3D promoter system, which is activated under sucrose-starved conditions, has emerged as a useful system for producing recombinant proteins. However, using rice as the production system for therapeutic proteins requires modifications of the N-glycosylation pattern because of the potential immunogenic...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2011.00636.x

    authors: Shin YJ,Chong YJ,Yang MS,Kwon TH

    更新日期:2011-12-01 00:00:00

  • Silencing the expression of the salivary sheath protein causes transgenerational feeding suppression in the aphid Sitobion avenae.

    abstract::Aphids produce gel saliva during feeding which forms a sheath around the stylet as it penetrates through the apoplast. The sheath is required for the sustained ingestion of phloem sap from sieve elements and is thought to form when the structural sheath protein (SHP) is cross-linked by intermolecular disulphide bridge...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12322

    authors: Abdellatef E,Will T,Koch A,Imani J,Vilcinskas A,Kogel KH

    更新日期:2015-08-01 00:00:00

  • Engineering rice with lower grain arsenic.

    abstract::Arsenic (As) is a poisonous element that causes severe skin lesions and cancer in humans. Rice (Oryza sativa L.) is a major dietary source of As in humans who consume this cereal as a staple food. We hypothesized that increasing As vacuolar sequestration would inhibit its translocation into the grain and reduce the am...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12905

    authors: Deng F,Yamaji N,Ma JF,Lee SK,Jeon JS,Martinoia E,Lee Y,Song WY

    更新日期:2018-10-01 00:00:00

  • OsNAC5 overexpression enlarges root diameter in rice plants leading to enhanced drought tolerance and increased grain yield in the field.

    abstract::Drought conditions are among the most serious challenges to crop production worldwide. Here, we report the results of field evaluations of transgenic rice plants overexpressing OsNAC5, under the control of either the root-specific (RCc3) or constitutive (GOS2) promoters. Field evaluations over three growing seasons re...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12011

    authors: Jeong JS,Kim YS,Redillas MC,Jang G,Jung H,Bang SW,Choi YD,Ha SH,Reuzeau C,Kim JK

    更新日期:2013-01-01 00:00:00

  • Overexpression of SrUGT76G1 in Stevia alters major steviol glycosides composition towards improved quality.

    abstract::Steviol glycosides (SGs) are extracted from Stevia leaves for use as a natural sweetener. Among SGs, stevioside is most abundant in leaf extracts followed by rebaudioside A (Reb A). However, Reb A is of particular interest because of its sweeter and more pleasant taste compared to stevioside. Therefore, the developmen...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13035

    authors: Kim MJ,Zheng J,Liao MH,Jang IC

    更新日期:2019-06-01 00:00:00

  • Fitness of Bt-resistant cabbage loopers on Bt cotton plants.

    abstract::Development of resistance to the insecticidal toxins from Bacillus thuringiensis (Bt) in insects is the major threat to the continued success of transgenic Bt crops in agriculture. The fitness of Bt-resistant insects on Bt and non-Bt plants is a key parameter that determines the development of Bt resistance in insect ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12718

    authors: Tetreau G,Wang R,Wang P

    更新日期:2017-10-01 00:00:00

  • An oligosaccharyltransferase from Leishmania major increases the N-glycan occupancy on recombinant glycoproteins produced in Nicotiana benthamiana.

    abstract::N-glycosylation is critical for recombinant glycoprotein production as it influences the heterogeneity of products and affects their biological function. In most eukaryotes, the oligosaccharyltransferase is the central-protein complex facilitating the N-glycosylation of proteins in the lumen of the endoplasmic reticul...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12906

    authors: Castilho A,Beihammer G,Pfeiffer C,Göritzer K,Montero-Morales L,Vavra U,Maresch D,Grünwald-Gruber C,Altmann F,Steinkellner H,Strasser R

    更新日期:2018-10-01 00:00:00

  • Comparative transcriptomics as a tool for the identification of root branching genes in maize.

    abstract::The root system is fundamental for plant development, is crucial for overall plant growth and is recently being recognized as the key for future crop productivity improvement. A major determinant of root system architecture is the initiation of lateral roots. While knowledge of the genetic and molecular mechanisms reg...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12104

    authors: Jansen L,Hollunder J,Roberts I,Forestan C,Fonteyne P,Van Quickenborne C,Zhen RG,McKersie B,Parizot B,Beeckman T

    更新日期:2013-12-01 00:00:00

  • Control limits for accumulation of plant metabolites: brute force is no substitute for understanding.

    abstract::Which factors limit metabolite accumulation in plant cells? Are theories on flux control effective at explaining the results? Many biotechnologists cling to the idea that every pathway has a rate limiting enzyme and target such enzymes first in order to modulate fluxes. This often translates into large effects on meta...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/pbi.12035

    authors: Morandini P

    更新日期:2013-02-01 00:00:00

  • A pathogenesis-related protein GmPR08-Bet VI promotes a molecular interaction between the GmSHMT08 and GmSNAP18 in resistance to Heterodera glycines.

    abstract::Soybean cyst nematode (SCN, Heterodera glycines) is the most devastating pest affecting soybean production worldwide. SCN resistance requires both the GmSHMT08 and the GmSNAP18 in 'Peking'-type resistance. Here, we describe the molecular interaction between GmSHMT08 and GmSNAP18, which is potentiated by a pathogenesis...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13343

    authors: Lakhssassi N,Piya S,Bekal S,Liu S,Zhou Z,Bergounioux C,Miao L,Meksem J,Lakhssassi A,Jones K,Kassem MA,Benhamed M,Bendahmane A,Lambert K,Boualem A,Hewezi T,Meksem K

    更新日期:2020-08-01 00:00:00

  • Tuber-specific cphA expression to enhance cyanophycin production in potatoes.

    abstract::The production of biodegradable polymers that can be used to substitute petrochemical compounds in commercial products in transgenic plants is an important challenge for plant biotechnology. Nevertheless, it is often accompanied by reduced plant fitness. To decrease the phenotypic abnormalities of the sprout and to in...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2009.00451.x

    authors: Hühns M,Neumann K,Hausmann T,Klemke F,Lockau W,Kahmann U,Kopertekh L,Staiger D,Pistorius EK,Reuther J,Waldvogel E,Wohlleben W,Effmert M,Junghans H,Neubauer K,Kragl U,Schmidt K,Schmidtke J,Broer I

    更新日期:2009-12-01 00:00:00

  • Overexpression of Hevea brasiliensis ethylene response factor HbERF-IXc5 enhances growth and tolerance to abiotic stress and affects laticifer differentiation.

    abstract::Ethylene response factor 1 (ERF1) is an essential integrator of the jasmonate and ethylene signalling pathways coordinating a large number of genes involved in plant defences. Its orthologue in Hevea brasiliensis, HbERF-IXc5, has been assumed to play a major role in laticifer metabolism and tolerance to harvesting str...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12774

    authors: Lestari R,Rio M,Martin F,Leclercq J,Woraathasin N,Roques S,Dessailly F,Clément-Vidal A,Sanier C,Fabre D,Melliti S,Suharsono S,Montoro P

    更新日期:2018-01-01 00:00:00

  • The overexpression of OsNAC9 alters the root architecture of rice plants enhancing drought resistance and grain yield under field conditions.

    abstract::Drought conditions limit agricultural production by preventing crops from reaching their genetically predetermined maximum yields. Here, we present the results of field evaluations of rice overexpressing OsNAC9, a member of the rice NAC domain family. Root-specific (RCc3) and constitutive (GOS2) promoters were used to...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2012.00697.x

    authors: Redillas MC,Jeong JS,Kim YS,Jung H,Bang SW,Choi YD,Ha SH,Reuzeau C,Kim JK

    更新日期:2012-09-01 00:00:00

  • Towards plant pangenomics.

    abstract::As an increasing number of genome sequences become available for a wide range of species, there is a growing understanding that the genome of a single individual is insufficient to represent the gene diversity within a whole species. Many studies examine the sequence diversity within genes, and this allelic variation ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/pbi.12499

    authors: Golicz AA,Batley J,Edwards D

    更新日期:2016-04-01 00:00:00

  • Tracking disease resistance deployment in potato breeding by enrichment sequencing.

    abstract::Following the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing (dRenSeq) enables the high-confidence i...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12997

    authors: Armstrong MR,Vossen J,Lim TY,Hutten RCB,Xu J,Strachan SM,Harrower B,Champouret N,Gilroy EM,Hein I

    更新日期:2019-02-01 00:00:00

  • Expression of a constitutively active nitrate reductase variant in tobacco reduces tobacco-specific nitrosamine accumulation in cured leaves and cigarette smoke.

    abstract::Burley tobaccos (Nicotiana tabacum) display a nitrogen-use-deficiency phenotype that is associated with the accumulation of high levels of nitrate within the leaf, a trait correlated with production of a class of compounds referred to as tobacco-specific nitrosamines (TSNAs). Two TSNA species, 4-(methylnitrosamino)-1-...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12510

    authors: Lu J,Zhang L,Lewis RS,Bovet L,Goepfert S,Jack AM,Crutchfield JD,Ji H,Dewey RE

    更新日期:2016-07-01 00:00:00

  • Using CRISPR/Cas9 genome editing in tomato to create a gibberellin-responsive dominant dwarf DELLA allele.

    abstract::The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12952

    authors: Tomlinson L,Yang Y,Emenecker R,Smoker M,Taylor J,Perkins S,Smith J,MacLean D,Olszewski NE,Jones JDG

    更新日期:2019-01-01 00:00:00

  • Metallothionein expression in chloroplasts enhances mercury accumulation and phytoremediation capability.

    abstract::Genetic engineering to enhance mercury phytoremediation has been accomplished by expression of the merAB genes that protects the cell by converting Hg[II] into Hg[0] which volatilizes from the cell. A drawback of this approach is that toxic Hg is released back into the environment. A better phytoremediation strategy w...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2011.00616.x

    authors: Ruiz ON,Alvarez D,Torres C,Roman L,Daniell H

    更新日期:2011-06-01 00:00:00

  • Whole-genome re-sequencing reveals the impact of the interaction of copy number variants of the rhg1 and Rhg4 genes on broad-based resistance to soybean cyst nematode.

    abstract::Soybean cyst nematode (SCN) is the most devastating plant-parasitic nematode. Most commercial soybean varieties with SCN resistance are derived from PI88788. Resistance derived from PI88788 is breaking down due to narrow genetic background and SCN population shift. PI88788 requires mainly the rhg1-b locus, while 'Peki...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13086

    authors: Patil GB,Lakhssassi N,Wan J,Song L,Zhou Z,Klepadlo M,Vuong TD,Stec AO,Kahil SS,Colantonio V,Valliyodan B,Rice JH,Piya S,Hewezi T,Stupar RM,Meksem K,Nguyen HT

    更新日期:2019-08-01 00:00:00

  • Forcing single-chain variable fragment production in tobacco seeds by fusion to elastin-like polypeptides.

    abstract::Single-chain variable fragments (scFvs), as well as other recombinant antibodies, have become powerful tools for therapy and analysis in both human and veterinary medicine. Transgenic plants have been developed as an efficient production system for these recombinant proteins, achieving high yields at moderate cost. Se...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2005.00176.x

    authors: Scheller J,Leps M,Conrad U

    更新日期:2006-03-01 00:00:00

  • Activity-based proteomics reveals nine target proteases for the recombinant protein-stabilizing inhibitor SlCYS8 in Nicotiana benthamiana.

    abstract::Co-expression of protease inhibitors like the tomato cystatin SlCYS8 is useful to increase recombinant protein production in plants, but key proteases involved in protein proteolysis are still unknown. Here, we performed activity-based protein profiling to identify proteases that are inhibited by SlCYS8 in agroinfiltr...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13092

    authors: Jutras PV,Grosse-Holz F,Kaschani F,Kaiser M,Michaud D,van der Hoorn RAL

    更新日期:2019-08-01 00:00:00

  • Three AtCesA6-like members enhance biomass production by distinctively promoting cell growth in Arabidopsis.

    abstract::Cellulose is an abundant biopolymer and a prominent constituent of plant cell walls. Cellulose is also a central component to plant morphogenesis and contributes the bulk of a plant's biomass. While cellulose synthase (CesA) genes were identified over two decades ago, genetic manipulation of this family to enhance cel...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12842

    authors: Hu H,Zhang R,Feng S,Wang Y,Wang Y,Fan C,Li Y,Liu Z,Schneider R,Xia T,Ding SY,Persson S,Peng L

    更新日期:2018-05-01 00:00:00

  • Domestication and the storage starch biosynthesis pathway: signatures of selection from a whole sorghum genome sequencing strategy.

    abstract::Next-generation sequencing of complete genomes has given researchers unprecedented levels of information to study the multifaceted evolutionary changes that have shaped elite plant germplasm. In conjunction with population genetic analytical techniques and detailed online databases, we can more accurately capture the ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12578

    authors: Campbell BC,Gilding EK,Mace ES,Tai S,Tao Y,Prentis PJ,Thomelin P,Jordan DR,Godwin ID

    更新日期:2016-12-01 00:00:00

  • Plant-produced candidate countermeasures against emerging and reemerging infections and bioterror agents.

    abstract::Despite progress in the prevention and treatment of infectious diseases, they continue to present a major threat to public health. The frequency of emerging and reemerging infections and the risk of bioterrorism warrant significant efforts towards the development of prophylactic and therapeutic countermeasures. Vaccin...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/pbi.12475

    authors: Streatfield SJ,Kushnir N,Yusibov V

    更新日期:2015-10-01 00:00:00

  • Agrobacterium-mediated RMCE approach for gene replacement.

    abstract::We describe the site-directed integration (SDI) system for Agrobacterium-mediated transformation to precisely integrate a single copy of a desired gene into a predefined target locus by recombinase-mediated cassette exchange (RMCE). The system requires the selection of a transformed line with an integrated copy of a t...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2005.00118.x

    authors: Nanto K,Yamada-Watanabe K,Ebinuma H

    更新日期:2005-03-01 00:00:00

  • Glycolate from microalgae: an efficient carbon source for biotechnological applications.

    abstract::Glycolate is produced in autotrophic cells under high temperatures and Ci -limitation via oxygenation of ribulose-1,5-bisphosphate. In unicellular algae, glycolate is lost via excretion or metabolized via the C2 cycle by consuming reductants, ATP and CO2 emission (photorespiration). Therefore, photorespiration is an i...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13078

    authors: Taubert A,Jakob T,Wilhelm C

    更新日期:2019-08-01 00:00:00

  • Biofortification of plants with altered antioxidant content and composition: genetic engineering strategies.

    abstract::Antioxidants are protective molecules that neutralize reactive oxygen species and prevent oxidative damage to cellular components such as membranes, proteins and nucleic acids, therefore reducing the rate of cell death and hence the effects of ageing and ageing-related diseases. The fortification of food with antioxid...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/j.1467-7652.2012.00740.x

    authors: Zhu C,Sanahuja G,Yuan D,Farré G,Arjó G,Berman J,Zorrilla-López U,Banakar R,Bai C,Pérez-Massot E,Bassie L,Capell T,Christou P

    更新日期:2013-02-01 00:00:00

  • The genetic architecture of the dynamic changes in grain moisture in maize.

    abstract::Low grain moisture at harvest is crucial for safe production, transport, and storage, but the genetic architecture of this trait in maize (Zea mays) remains elusive. Here, we measured the dynamic changes in grain moisture content in an association mapping panel of 513 diverse maize inbred lines at five successive stag...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13541

    authors: Li W,Yu Y,Wang L,Luo Y,Peng Y,Xu Y,Liu X,Wu S,Jian L,Xu J,Xiao Y,Yan J

    更新日期:2021-01-01 00:00:00

  • Down-regulated expression of plant-specific glycoepitopes in alfalfa.

    abstract:SUMMARY:Compared with other plant expression systems used for pharmaceutical protein production, alfalfa offers the advantage of very homogeneous N-glycosylation. Therefore, this plant was selected for further attempts at glycoengineering. Two main approaches were developed in order to humanize N-glycosylation in alfal...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2008.00353.x

    authors: Sourrouille C,Marquet-Blouin E,D'Aoust MA,Kiefer-Meyer MC,Seveno M,Pagny-Salehabadi S,Bardor M,Durambur G,Lerouge P,Vezina L,Gomord V

    更新日期:2008-09-01 00:00:00