Engineered selective plant male sterility through pollen-specific expression of the EcoRI restriction endonuclease.

Abstract:

:Unintended gene flow from transgenic plants via pollen, seed and vegetative propagation is a regulatory concern because of potential admixture in food and crop systems, as well as hybridization and introgression to wild and weedy relatives. Bioconfinement of transgenic pollen would help address some of these concerns and enable transgenic plant production for several crops where gene flow is an issue. Here, we demonstrate the expression of the restriction endonuclease EcoRI under the control of the tomato pollen-specific LAT52 promoter is an effective method for generating selective male sterility in Nicotiana tabacum (tobacco). Of nine transgenic events recovered, four events had very high bioconfinement with tightly controlled EcoRI expression in pollen and negligible-to-no expression other plant tissues. Transgenic plants had normal morphology wherein vegetative growth and reproductivity were similar to nontransgenic controls. In glasshouse experiments, transgenic lines were hand-crossed to both male-sterile and emasculated nontransgenic tobacco varieties. Progeny analysis of 16 000-40 000 seeds per transgenic line demonstrated five lines approached (>99.7%) or attained 100% bioconfinement for one or more generations. Bioconfinement was again demonstrated at or near 100% under field conditions where four transgenic lines were grown in close proximity to male-sterile tobacco, and 900-2100 seeds per male-sterile line were analysed for transgenes. Based upon these results, we conclude EcoRI-driven selective male sterility holds practical potential as a safe and reliable transgene bioconfinement strategy. Given the mechanism of male sterility, this method could be applicable to any plant species.

journal_name

Plant Biotechnol J

authors

Millwood RJ,Moon HS,Poovaiah CR,Muthukumar B,Rice JH,Abercrombie JM,Abercrombie LL,Green WD,Stewart CN Jr

doi

10.1111/pbi.12493

subject

Has Abstract

pub_date

2016-05-01 00:00:00

pages

1281-90

issue

5

eissn

1467-7644

issn

1467-7652

journal_volume

14

pub_type

杂志文章
  • Functional analysis of soybean genes involved in flavonoid biosynthesis by virus-induced gene silencing.

    abstract::Virus-induced gene silencing (VIGS) is a powerful tool for functional analysis of genes in plants. A wide-host-range VIGS vector, which was developed based on the Cucumber mosaic virus (CMV), was tested for its ability to silence endogenous genes involved in flavonoid biosynthesis in soybean. Symptomless infection was...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2007.00288.x

    authors: Nagamatsu A,Masuta C,Senda M,Matsuura H,Kasai A,Hong JS,Kitamura K,Abe J,Kanazawa A

    更新日期:2007-11-01 00:00:00

  • ZFN-induced mutagenesis and gene-targeting in Arabidopsis through Agrobacterium-mediated floral dip transformation.

    abstract::Zinc-finger nucleases (ZFNs) are artificial restriction enzymes, custom designed for induction of double-strand breaks (DSBs) at a specific locus. These DSBs may result in site-specific mutagenesis or homologous recombination at the repair site, depending on the DNA repair pathway that is used. These promising techniq...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2009.00446.x

    authors: de Pater S,Neuteboom LW,Pinas JE,Hooykaas PJ,van der Zaal BJ

    更新日期:2009-10-01 00:00:00

  • Induction and recovery of copy number variation in banana through gamma irradiation and low-coverage whole-genome sequencing.

    abstract::Traditional breeding methods are hindered in bananas due to the fact that major cultivars are sterile, parthenocarpic, triploid and thus clonally propagated. This has resulted in a narrow genetic base and limited resilience to biotic and abiotic stresses. Mutagenesis of in vitro propagated bananas is one method to int...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12901

    authors: Datta S,Jankowicz-Cieslak J,Nielen S,Ingelbrecht I,Till BJ

    更新日期:2018-02-24 00:00:00

  • The pomegranate (Punica granatum L.) genome provides insights into fruit quality and ovule developmental biology.

    abstract::Pomegranate (Punica granatum L.) has an ancient cultivation history and has become an emerging profitable fruit crop due to its attractive features such as the bright red appearance and the high abundance of medicinally valuable ellagitannin-based compounds in its peel and aril. However, the limited genomic resources ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12875

    authors: Yuan Z,Fang Y,Zhang T,Fei Z,Han F,Liu C,Liu M,Xiao W,Zhang W,Wu S,Zhang M,Ju Y,Xu H,Dai H,Liu Y,Chen Y,Wang L,Zhou J,Guan D,Yan M,Xia Y,Huang X,Liu D,Wei H,Zheng H

    更新日期:2018-07-01 00:00:00

  • Developing xylem-preferential expression of PdGA20ox1, a gibberellin 20-oxidase 1 from Pinus densiflora, improves woody biomass production in a hybrid poplar.

    abstract::Woody biomass has gained popularity as an environmentally friendly, renewable and sustainable resource for liquid fuel production. Here, we demonstrate biotechnological improvement of the quantity and quality of woody biomass by employing developing xylem (DX)-preferential production of gibberellin (GA), a phytohormon...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12484

    authors: Jeon HW,Cho JS,Park EJ,Han KH,Choi YI,Ko JH

    更新日期:2016-04-01 00:00:00

  • A global view of transcriptome dynamics during flower development in chickpea by deep sequencing.

    abstract::Measurement of gene expression can provide important clues about gene function and molecular basis of developmental processes. Here, we have analysed the chickpea transcriptome in vegetative and flower tissues by exploiting the potential of high-throughput sequencing to measure gene expression. We mapped more than 295...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12059

    authors: Singh VK,Garg R,Jain M

    更新日期:2013-08-01 00:00:00

  • A hybrid Bacillus thuringiensis delta-endotoxin gives resistance against a coleopteran and a lepidopteran pest in transgenic potato.

    abstract::Expression of Bacillus thuringiensis delta-endotoxins has proven to be a successful strategy for obtaining insect resistance in transgenic plants. Drawbacks of expression of a single resistance gene are the limited target spectrum and the potential for rapid adaptation of the pest. Hybrid toxins with a wider target sp...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1046/j.1467-7652.2003.00005.x

    authors: Naimov S,Dukiandjiev S,de Maagd RA

    更新日期:2003-01-01 00:00:00

  • Immunotherapy using algal-produced Ara h 1 core domain suppresses peanut allergy in mice.

    abstract::Peanut allergy is an IgE-mediated adverse reaction to a subset of proteins found in peanuts. Immunotherapy aims to desensitize allergic patients through repeated and escalating exposures for several months to years using extracts or flours. The complex mix of proteins and variability between preparations complicates i...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12515

    authors: Gregory JA,Shepley-McTaggart A,Umpierrez M,Hurlburt BK,Maleki SJ,Sampson HA,Mayfield SP,Berin MC

    更新日期:2016-07-01 00:00:00

  • Co-expression of tonoplast Cation/H(+) antiporter and H(+)-pyrophosphatase from xerophyte Zygophyllum xanthoxylum improves alfalfa plant growth under salinity, drought and field conditions.

    abstract::Salinity and drought are major environmental factors limiting the growth and productivity of alfalfa worldwide as this economically important legume forage is sensitive to these kinds of abiotic stress. In this study, transgenic alfalfa lines expressing both tonoplast NXH and H(+)-PPase genes, ZxNHX and ZxVP1-1 from t...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12451

    authors: Bao AK,Du BQ,Touil L,Kang P,Wang QL,Wang SM

    更新日期:2016-03-01 00:00:00

  • Coupling two mercury resistance genes in Eastern cottonwood enhances the processing of organomercury.

    abstract::Eastern cottonwood (Populus deltoides Bartr. ex Marsh.) trees were engineered to express merA (mercuric ion reductase) and merB (organomercury lyase) transgenes in order to be used for the phytoremediation of mercury-contaminated soils. Earlier studies with Arabidopsis thaliana and Nicotiana tabacum showed that this g...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2006.00236.x

    authors: Lyyra S,Meagher RB,Kim T,Heaton A,Montello P,Balish RS,Merkle SA

    更新日期:2007-03-01 00:00:00

  • Metallothionein expression in chloroplasts enhances mercury accumulation and phytoremediation capability.

    abstract::Genetic engineering to enhance mercury phytoremediation has been accomplished by expression of the merAB genes that protects the cell by converting Hg[II] into Hg[0] which volatilizes from the cell. A drawback of this approach is that toxic Hg is released back into the environment. A better phytoremediation strategy w...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2011.00616.x

    authors: Ruiz ON,Alvarez D,Torres C,Roman L,Daniell H

    更新日期:2011-06-01 00:00:00

  • Glycolate from microalgae: an efficient carbon source for biotechnological applications.

    abstract::Glycolate is produced in autotrophic cells under high temperatures and Ci -limitation via oxygenation of ribulose-1,5-bisphosphate. In unicellular algae, glycolate is lost via excretion or metabolized via the C2 cycle by consuming reductants, ATP and CO2 emission (photorespiration). Therefore, photorespiration is an i...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13078

    authors: Taubert A,Jakob T,Wilhelm C

    更新日期:2019-08-01 00:00:00

  • Transcript-specific, single-nucleotide polymorphism discovery and linkage analysis in hexaploid bread wheat (Triticum aestivum L.).

    abstract::Food security is a global concern and substantial yield increases in cereal crops are required to feed the growing world population. Wheat is one of the three most important crops for human and livestock feed. However, the complexity of the genome coupled with a decline in genetic diversity within modern elite cultiva...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2011.00628.x

    authors: Allen AM,Barker GL,Berry ST,Coghill JA,Gwilliam R,Kirby S,Robinson P,Brenchley RC,D'Amore R,McKenzie N,Waite D,Hall A,Bevan M,Hall N,Edwards KJ

    更新日期:2011-12-01 00:00:00

  • Whole-genome re-sequencing reveals the impact of the interaction of copy number variants of the rhg1 and Rhg4 genes on broad-based resistance to soybean cyst nematode.

    abstract::Soybean cyst nematode (SCN) is the most devastating plant-parasitic nematode. Most commercial soybean varieties with SCN resistance are derived from PI88788. Resistance derived from PI88788 is breaking down due to narrow genetic background and SCN population shift. PI88788 requires mainly the rhg1-b locus, while 'Peki...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13086

    authors: Patil GB,Lakhssassi N,Wan J,Song L,Zhou Z,Klepadlo M,Vuong TD,Stec AO,Kahil SS,Colantonio V,Valliyodan B,Rice JH,Piya S,Hewezi T,Stupar RM,Meksem K,Nguyen HT

    更新日期:2019-08-01 00:00:00

  • Production of tetravalent dengue virus envelope protein domain III based antigens in lettuce chloroplasts and immunologic analysis for future oral vaccine development.

    abstract::Dengue fever is a mosquito (Aedes aegypti) -transmitted viral disease that is endemic in more than 125 countries around the world. There are four serotypes of the dengue virus (DENV 1-4) and a safe and effective dengue vaccine must provide protection against all four serotypes. To date, the first vaccine, Dengvaxia (C...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13065

    authors: van Eerde A,Gottschamel J,Bock R,Hansen KEA,Munang'andu HM,Daniell H,Liu Clarke J

    更新日期:2019-07-01 00:00:00

  • Fitness of Bt-resistant cabbage loopers on Bt cotton plants.

    abstract::Development of resistance to the insecticidal toxins from Bacillus thuringiensis (Bt) in insects is the major threat to the continued success of transgenic Bt crops in agriculture. The fitness of Bt-resistant insects on Bt and non-Bt plants is a key parameter that determines the development of Bt resistance in insect ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12718

    authors: Tetreau G,Wang R,Wang P

    更新日期:2017-10-01 00:00:00

  • Agrobacterium tumefaciens-mediated sorghum transformation using a mannose selection system.

    abstract::A dual-marker plasmid containing the selectable marker gene, manA, and the reporter gene, sgfp, was used to transform immature sorghum embryos by employing an Agrobacterium-mediated system. Both genes were under the control of the ubi1 promoter in a binary vector pPZP201. The Escherichia coli phosphomannose isomerase ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2005.00150.x

    authors: Gao Z,Xie X,Ling Y,Muthukrishnan S,Liang GH

    更新日期:2005-11-01 00:00:00

  • Control limits for accumulation of plant metabolites: brute force is no substitute for understanding.

    abstract::Which factors limit metabolite accumulation in plant cells? Are theories on flux control effective at explaining the results? Many biotechnologists cling to the idea that every pathway has a rate limiting enzyme and target such enzymes first in order to modulate fluxes. This often translates into large effects on meta...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/pbi.12035

    authors: Morandini P

    更新日期:2013-02-01 00:00:00

  • Transient expression assays in grapevine: a step towards genetic improvement.

    abstract::In the past few years, the usefulness of transient expression assays has continuously increased for the characterization of unknown gene function and metabolic pathways. In grapevine (Vitis vinifera L.), one of the most economically important fruit crops in the world, recent systematic sequencing projects produced man...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/pbi.12294

    authors: Jelly NS,Valat L,Walter B,Maillot P

    更新日期:2014-12-01 00:00:00

  • Genomic analyses of diverse wild and cultivated accessions provide insights into the evolutionary history of jujube.

    abstract::The Chinese jujube (Ziziphus jujuba Mill.), a member of the Rhamnaceae family, is an important perennial fruit tree crop of substantial economic, ecological and nutritional value, and is also used as a traditional herbal medicine. Here, we report the resequencing of 493 jujube accessions, including 202 wild and 291 cu...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13480

    authors: Guo M,Zhang Z,Li S,Lian Q,Fu P,He Y,Qiao J,Xu K,Liu L,Wu M,Du Z,Li S,Wang J,Shao P,Yu Q,Xu G,Li D,Wang Y,Tian S,Zhao J,Feng X,Li R,Jiang W,Zhao X

    更新日期:2020-09-18 00:00:00

  • Regulatory approval and a first-in-human phase I clinical trial of a monoclonal antibody produced in transgenic tobacco plants.

    abstract::Although plant biotechnology has been widely investigated for the production of clinical-grade monoclonal antibodies, no antibody products derived from transgenic plants have yet been approved by pharmaceutical regulators for clinical testing. In the Pharma-Planta project, the HIV-neutralizing human monoclonal antibod...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/pbi.12416

    authors: Ma JK,Drossard J,Lewis D,Altmann F,Boyle J,Christou P,Cole T,Dale P,van Dolleweerd CJ,Isitt V,Katinger D,Lobedan M,Mertens H,Paul MJ,Rademacher T,Sack M,Hundleby PA,Stiegler G,Stoger E,Twyman RM,Vcelar B,Fischer

    更新日期:2015-10-01 00:00:00

  • The in silico identification and characterization of a bread wheat/Triticum militinae introgression line.

    abstract::The capacity of the bread wheat (Triticum aestivum) genome to tolerate introgression from related genomes can be exploited for wheat improvement. A resistance to powdery mildew expressed by a derivative of the cross-bread wheat cv. Tähti × T. militinae (Tm) is known to be due to the incorporation of a Tm segment into ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12610

    authors: Abrouk M,Balcárková B,Šimková H,Komínkova E,Martis MM,Jakobson I,Timofejeva L,Rey E,Vrána J,Kilian A,Järve K,Doležel J,Valárik M

    更新日期:2017-02-01 00:00:00

  • Oligonucleotide-directed mutagenesis for precision gene editing.

    abstract::Differences in gene sequences, many of which are single nucleotide polymorphisms, underlie some of the most important traits in plants. With humanity facing significant challenges to increase global agricultural productivity, there is an urgent need to accelerate the development of these traits in plants. oligonucleot...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/pbi.12496

    authors: Sauer NJ,Mozoruk J,Miller RB,Warburg ZJ,Walker KA,Beetham PR,Schöpke CR,Gocal GF

    更新日期:2016-02-01 00:00:00

  • CRISPR/Cas9-mediated knockout of Ms1 enables the rapid generation of male-sterile hexaploid wheat lines for use in hybrid seed production.

    abstract::The development and adoption of hybrid seed technology have led to dramatic increases in agricultural productivity. However, it has been a challenge to develop a commercially viable platform for the production of hybrid wheat (Triticum aestivum) seed due to wheat's strong inbreeding habit. Recently, a novel platform f...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.13106

    authors: Okada A,Arndell T,Borisjuk N,Sharma N,Watson-Haigh NS,Tucker EJ,Baumann U,Langridge P,Whitford R

    更新日期:2019-10-01 00:00:00

  • Gene silencing of mannose 6-phosphate reductase in the parasitic weed Orobanche aegyptiaca through the production of homologous dsRNA sequences in the host plant.

    abstract::Orobanche spp. (broomrape) are parasitic plants which subsist on the roots of a wide range of hosts, including tomato, causing severe losses in yield quality and quantity. Large amounts of mannitol accumulate in this parasitic weed during development. Mannose 6-phosphate reductase (M6PR) is a key enzyme in mannitol bi...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2009.00418.x

    authors: Aly R,Cholakh H,Joel DM,Leibman D,Steinitz B,Zelcer A,Naglis A,Yarden O,Gal-On A

    更新日期:2009-08-01 00:00:00

  • The origins and progress of genomics research on Tef (Eragrostis tef).

    abstract::Tef, Eragrostis tef (Zucc.) Trotter, is the most important cereal in Ethiopia. Tef is cultivated by more than five million small-scale farmers annually and constitutes the staple food for more than half of the population of 80 million. The crop is preferred by both farmers and consumers due to its beneficial traits as...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审

    doi:10.1111/pbi.12199

    authors: Girma D,Assefa K,Chanyalew S,Cannarozzi G,Kuhlemeier C,Tadele Z

    更新日期:2014-06-01 00:00:00

  • ZmSTK1 and ZmSTK2, encoding receptor-like cytoplasmic kinase, are involved in maize pollen development with additive effect.

    abstract::Pollen germination and pollen tube growth are important physiological processes of sexual reproduction of plants and also are involved in signal transduction. Our previous study reveals that ZmSTK1 and ZmSTK2 are two receptor-like cytoplasmic kinases (RLCK) homologs in Zea mays as members of receptor-like protein kina...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12880

    authors: Fan M,Zhang C,Shi L,Liu C,Ma W,Chen M,Liu K,Cai F,Wang G,Wei Z,Jiang M,Liu Z,Javeed A,Lin F

    更新日期:2018-08-01 00:00:00

  • Suppression of α-amylase genes improves quality of rice grain ripened under high temperature.

    abstract::High temperature impairs rice (Oryza sativa) grain filling by inhibiting the deposition of storage materials such as starch, resulting in mature grains with a chalky appearance, currently a major problem for rice farming in Asian countries. Such deterioration of grain quality is accompanied by the altered expression o...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2012.00741.x

    authors: Hakata M,Kuroda M,Miyashita T,Yamaguchi T,Kojima M,Sakakibara H,Mitsui T,Yamakawa H

    更新日期:2012-12-01 00:00:00

  • Enhancing the expression of starch synthase class IV results in increased levels of both transitory and long-term storage starch.

    abstract::Starch is an important renewable raw material with an increasing number of applications. Several attempts have been made to obtain plants that produce modified versions of starch or higher starch yield. Most of the approaches designed to increase the levels of starch have focused on the increment of the amount of ADP-...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/j.1467-7652.2011.00626.x

    authors: Gámez-Arjona FM,Li J,Raynaud S,Baroja-Fernández E,Muñoz FJ,Ovecka M,Ragel P,Bahaji A,Pozueta-Romero J,Mérida Á

    更新日期:2011-12-01 00:00:00

  • Discovery and development of exome-based, co-dominant single nucleotide polymorphism markers in hexaploid wheat (Triticum aestivum L.).

    abstract::Globally, wheat is the most widely grown crop and one of the three most important crops for human and livestock feed. However, the complex nature of the wheat genome has, until recently, resulted in a lack of single nucleotide polymorphism (SNP)-based molecular markers of practical use to wheat breeders. Recently, lar...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章

    doi:10.1111/pbi.12009

    authors: Allen AM,Barker GL,Wilkinson P,Burridge A,Winfield M,Coghill J,Uauy C,Griffiths S,Jack P,Berry S,Werner P,Melichar JP,McDougall J,Gwilliam R,Robinson P,Edwards KJ

    更新日期:2013-04-01 00:00:00