Abstract:
:Genetic engineering with just a few genes has changed agriculture in the last 20 years. The most frequently used transgenes are the herbicide resistance genes for efficient weed control and the Bt toxin genes for insect resistance. The adoption of the first-generation genetically engineered crops has been very successful in improving farming practices, reducing the application of pesticides that are harmful to both human health and the environment, and producing more profit for farmers. However, there is more potential for genetic engineering to be realized by technical advances. The recent development of plant artificial chromosome technology provides a super vector platform, which allows the management of a large number of genes for the next generation of genetic engineering. With the development of other tools such as gene assembly, genome editing, gene targeting and chromosome delivery systems, it should become possible to engineer crops with multiple genes to produce more agricultural products with less input of natural resources to meet future demands.
journal_name
Plant Biotechnol Jjournal_title
Plant biotechnology journalauthors
Yu W,Yau YY,Birchler JAdoi
10.1111/pbi.12466subject
Has Abstractpub_date
2016-05-01 00:00:00pages
1175-82issue
5eissn
1467-7644issn
1467-7652journal_volume
14pub_type
杂志文章,评审abstract::Designing transformation experiments for either functional genomics or crop improvement requires knowledge of the transgene locus structure, number, transmission and expression resulting from a specific transformation method. We recently reported an improvement to the soybean [Glycine max (L.) Merrill] cotyledonary-no...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2004.00070.x
更新日期:2004-07-01 00:00:00
abstract::The replacement of crude allergen extracts by selected allergens currently represents a major goal for the improvement of allergy diagnosis and immunotherapy. Indeed, the development of molecularly defined vaccines would facilitate both standardization and enhance batch-to-batch reproducibility as well as treatment sp...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2006.00221.x
更新日期:2007-01-01 00:00:00
abstract::The related A genome species of the Oryza genus are the effective gene pool for rice. Here, we report draft genomes for two Australian wild A genome taxa: O. rufipogon-like population, referred to as Taxon A, and O. meridionalis-like population, referred to as Taxon B. These two taxa were sequenced and assembled by in...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12674
更新日期:2017-06-01 00:00:00
abstract::To infect plants, viruses rely heavily on their host's machinery. Plant genetic resistances based on host factor modifications can be found among existing natural variability and are widely used for some but not all crops. While biotechnology can supply for the lack of natural resistance alleles, new strategies need t...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12896
更新日期:2018-03-05 00:00:00
abstract::The nitrate (NO3-) transporter has been selected as an important gene maker in the process of environmental adoption in rice cultivars. In this work, we transferred another native OsNAR2.1 promoter with driving OsNAR2.1 gene into rice plants. The transgenic lines with exogenous pOsNAR2.1:OsNAR2.1 constructs showed enh...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12714
更新日期:2017-10-01 00:00:00
abstract::The assembly of a reference genome sequence of bread wheat is challenging due to its specific features such as the genome size of 17 Gbp, polyploid nature and prevalence of repetitive sequences. BAC-by-BAC sequencing based on chromosomal physical maps, adopted by the International Wheat Genome Sequencing Consortium as...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12513
更新日期:2016-07-01 00:00:00
abstract::In the past few years, the usefulness of transient expression assays has continuously increased for the characterization of unknown gene function and metabolic pathways. In grapevine (Vitis vinifera L.), one of the most economically important fruit crops in the world, recent systematic sequencing projects produced man...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/pbi.12294
更新日期:2014-12-01 00:00:00
abstract::The cooking quality of rice is associated with the starch gelatinization temperature (GT). Rice genotypes with low GT have probably been selected for their cooking quality by humans during domestication. We now report polymorphisms in starch synthase IIa (SSIIa) that explain the variation in rice starch GT. Sequence a...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2005.00162.x
更新日期:2006-01-01 00:00:00
abstract::There is an urgent need to provide effective anti-HIV microbicides to resource-poor areas worldwide. Some of the most promising microbicide candidates are biotherapeutics targeting viral entry. To provide biotherapeutics to poorer areas, it is vital to reduce the cost. Here, we report the production of biologically ac...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12309
更新日期:2015-09-01 00:00:00
abstract::Traditional breeding methods are hindered in bananas due to the fact that major cultivars are sterile, parthenocarpic, triploid and thus clonally propagated. This has resulted in a narrow genetic base and limited resilience to biotic and abiotic stresses. Mutagenesis of in vitro propagated bananas is one method to int...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12901
更新日期:2018-02-24 00:00:00
abstract::Plant chloroplasts are promising vehicles for recombinant protein production, but the process of protein folding in these organelles is not well understood in comparison with that in prokaryotic systems, such as Escherichia coli. This is particularly true for disulphide bond formation which is crucial for the biologic...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2007.00298.x
更新日期:2008-01-01 00:00:00
abstract::DNA recombination reactions (site-specific and homologous) were monitored in the progeny of transgenic maize plants by bringing together two recombination substrates (docking sites and shuttle vectors) in the zygotes. In one combination of transgenic events, the recombination marker gene (yellow fluorescent protein ge...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2006.00186.x
更新日期:2006-05-01 00:00:00
abstract::Genetically engineered (GE) rice lines expressing Lepidoptera-active insecticidal cry genes from the bacterium Bacillus thuringiensis (Bt) have been developed in China. Field surveys indicated that Bt rice harbours fewer rice planthoppers than non-Bt rice although planthoppers are not sensitive to the produced Bt Cry ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12911
更新日期:2018-10-01 00:00:00
abstract::A high throughput proton nuclear magnetic resonance spectroscopy method for the metabolite fingerprinting of plants was applied to genetically modified peas (Pisum sativum) to determine whether biochemical changes, so called 'unintended effects', beyond those intended by incorporation of a transgene, were detectable. ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1046/j.1467-7652.2003.00045.x
更新日期:2004-01-01 00:00:00
abstract::The development and adoption of hybrid seed technology have led to dramatic increases in agricultural productivity. However, it has been a challenge to develop a commercially viable platform for the production of hybrid wheat (Triticum aestivum) seed due to wheat's strong inbreeding habit. Recently, a novel platform f...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13106
更新日期:2019-10-01 00:00:00
abstract::The importance and urgency of providing humans and animals with quality proteins are reflected in the growing scientific and industrial interest in augmenting the nutritive value of the world's protein sources. Such nutritive value is determined by the protein content in 'essential amino acids', those that cannot be s...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/j.1467-7652.2006.00204.x
更新日期:2006-09-01 00:00:00
abstract::Glycine betaine plays an important role in some plants, including maize, in conditions of abiotic stress, but different maize varieties vary in their capacity to accumulate glycine betaine. An elite maize inbred line DH4866 was transformed with the betA gene from Escherichia coli encoding choline dehydrogenase (EC 1.1...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2004.00093.x
更新日期:2004-11-01 00:00:00
abstract::Grain size, number and starch content are important determinants of grain yield and quality. One of the most important biological processes that determine these components is the carbon partitioning during the early grain filling, which requires the function of cell wall invertase. Here, we showed the constitutive exp...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12102
更新日期:2013-12-01 00:00:00
abstract::There is a pressing need to develop novel antibacterial agents given the widespread antibiotic resistance among pathogenic bacteria and the low specificity of the drugs available. Endolysins are antibacterial proteins that are produced by bacteriophage-infected cells to digest the bacterial cell wall for phage progeny...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12703
更新日期:2017-09-01 00:00:00
abstract::Tomato is one of the most cultivated vegetables in the world and an important ingredient of the human diet. Tomato breeders and growers face a continuous challenge of combining high quantity (production volume) with high quality (appearance, taste and perception for the consumers, processing quality for the processing...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12268
更新日期:2015-02-01 00:00:00
abstract::Genome modification by homology-directed repair (HDR) is an attractive tool for the controlled genetic manipulation of plants. Here, we report the HDR-mediated gene exchange of expression cassettes in tobacco BY-2 cells using a designed zinc finger nuclease (ZFN). The target contained a 7-kb fragment flanked by two ZF...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12483
更新日期:2016-04-01 00:00:00
abstract::Increased use of nitrogen fertilizers has deleterious impact on the environment. Increase in yield potential at low nitrogen supply is regarded as a cereal breeding goal for future agricultural sustainability. Although natural variations of nitrogen transporters have been investigated, key genes associated with assimi...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.13450
更新日期:2021-01-01 00:00:00
abstract::Direct control of protein level enables rapid and efficient analyses of gene functions in crops. Previously, we developed the RDDK-Shield1 (Shld1) system in the model plant Arabidopsis thaliana for direct modulation of protein stabilization using a synthetic small molecule. However, it was unclear whether this system ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12787
更新日期:2018-02-01 00:00:00
abstract::The 1980s and 1990s saw a major expansion of biotechnology into new areas of science including genomics and recombinant technologies. This was coupled to the widespread emergence of academics into the commercial sector as they were encouraged to spin out companies or commercialize their intellectual property. There we...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/pbi.12426
更新日期:2015-10-01 00:00:00
abstract::Flavonoids are widely distributed in plants and play important roles in human and animal health and nutrition. Model plants with discernible flavonoid phenotypes, such as Arabidopsis seed patterning lines, are valuable tools that can provide avenues for understanding flavonoid and proanthocyanidin accumulation pattern...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2010.00525.x
更新日期:2010-12-01 00:00:00
abstract::Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer c...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12359
更新日期:2016-01-01 00:00:00
abstract::Production of novel transgenic floricultural crops with altered petal properties requires transgenes that confer a useful trait and petal-specific promoters. Several promoters have been shown to control transgenes in petals. However, all suffer from inherent drawbacks such as low petal specificity and restricted activ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12389
更新日期:2016-01-01 00:00:00
abstract::2-Acetyl-1-pyrroline (2AP), the volatile compound that provides the 'popcorn-like' aroma in a large variety of cereal and food products, is widely found in nature. Deficiency in amino aldehyde dehydrogenase (AMADH) was previously shown to be the likely cause of 2AP biosynthesis in rice (Oryza sativa L.). In this study...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2010.00533.x
更新日期:2011-01-01 00:00:00
abstract::Differences in gene sequences, many of which are single nucleotide polymorphisms, underlie some of the most important traits in plants. With humanity facing significant challenges to increase global agricultural productivity, there is an urgent need to accelerate the development of these traits in plants. oligonucleot...
journal_title:Plant biotechnology journal
pub_type: 杂志文章,评审
doi:10.1111/pbi.12496
更新日期:2016-02-01 00:00:00
abstract::The tissue-specific expression of transgenes is essential in plant breeding programmes to avoid the fitness costs caused by constitutive expression of a target gene. However, knowledge on the molecular mechanisms of tissue-specific gene expression and practicable tissue-specific promoters is limited. In this study, we...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2007.00271.x
更新日期:2007-09-01 00:00:00