PpGST1, an anthocyanin-related glutathione S-transferase gene, is essential for fruit coloration in peach.


:Anthocyanins have crucial biological functions and affect quality of horticultural produce. Anthocyanins accumulate in ripe peach fruit; differential accumulation is observed in deep coloured cultivar 'Hujingmilu' and lightly pigmented cultivar 'Yulu'. The difference was not fully explained by accumulation of total flavonoids and expression of anthocyanin biosynthetic genes. Expression analysis was conducted on a glutathione S-transferase gene (PpGST1), and it was found that the expression correlated well with anthocyanin accumulation in peach fruit tissues. Functional complementation of the Arabidopsis tt19 mutant indicated that PpGST1 was responsible for transport of anthocyanins but not proanthocyanidins. PpGST1 was localized in nuclei and the tonoplast, including the sites at which anthocyanin vacuolar sequestration occurred. Transient overexpression of PpGST1 together with PpMYB10.1 in tobacco leaves and peach fruit significantly increased anthocyanin accumulation as compared with PpMYB10.1 alone. Furthermore, virus-induced gene silencing of PpGST1 in a blood-fleshed peach not only resulted in a reduction in anthocyanin accumulation but also a decline in expression of anthocyanin biosynthetic and regulatory genes. Cis-element analysis of the PpGST1 promoter revealed the presence of four MYB binding sites (MBSs). Dual-luciferase assays indicated that PpMYB10.1 bound to the promoter and activated the transcription of PpGST1 by recognizing MBS1, the one closest to the ATG start codon, with this trans-activation being stronger against the promoter of deep coloured 'Hujingmilu' compared with lightly coloured cultivar 'Yulu'. Altogether, our data provided molecular evidence supporting coordinative regulatory roles of PpGST1 and PpMYB10.1 in anthocyanin accumulation in peach.


Plant Biotechnol J


Zhao Y,Dong W,Zhu Y,Allan AC,Lin-Wang K,Xu C




Has Abstract


2020-05-01 00:00:00












  • Altered lignin biosynthesis using biotechnology to improve lignocellulosic biofuel feedstocks.

    abstract::Lignocellulosic feedstocks can be converted to biofuels, which can conceivably replace a large fraction of fossil fuels currently used for transformation. However, lignin, a prominent constituent of secondary cell walls, is an impediment to the conversion of cell walls to fuel: the recalcitrance problem. Biomass pretr...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审


    authors: Poovaiah CR,Nageswara-Rao M,Soneji JR,Baxter HL,Stewart CN Jr

    更新日期:2014-12-01 00:00:00

  • Functional analysis of soybean genes involved in flavonoid biosynthesis by virus-induced gene silencing.

    abstract::Virus-induced gene silencing (VIGS) is a powerful tool for functional analysis of genes in plants. A wide-host-range VIGS vector, which was developed based on the Cucumber mosaic virus (CMV), was tested for its ability to silence endogenous genes involved in flavonoid biosynthesis in soybean. Symptomless infection was...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Nagamatsu A,Masuta C,Senda M,Matsuura H,Kasai A,Hong JS,Kitamura K,Abe J,Kanazawa A

    更新日期:2007-11-01 00:00:00

  • High-resolution expression profiling of selected gene sets during plant immune activation.

    abstract::The plant immune system involves detection of pathogens via both cell-surface and intracellular receptors. Both receptor classes can induce transcriptional reprogramming that elevates disease resistance. To assess differential gene expression during plant immunity, we developed and deployed quantitative sequence captu...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Ding P,Ngou BPM,Furzer OJ,Sakai T,Shrestha RK,MacLean D,Jones JDG

    更新日期:2020-07-01 00:00:00

  • Plant peptides and peptidomics.

    abstract::Extracellular plant peptides perform a large variety of functions, including signalling and defence. Intracellular peptides often have physiological functions or may merely be the products of general proteolysis. Plant peptides have been identified and, in part, functionally characterized through biochemical and genet...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审


    authors: Farrokhi N,Whitelegge JP,Brusslan JA

    更新日期:2008-02-01 00:00:00

  • Targeted molecular trait stacking in cotton through targeted double-strand break induction.

    abstract::Recent developments of tools for targeted genome modification have led to new concepts in how multiple traits can be combined. Targeted genome modification is based on the use of nucleases with tailor-made specificities to introduce a DNA double-strand break (DSB) at specific target loci. A re-engineered meganuclease ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: D'Halluin K,Vanderstraeten C,Van Hulle J,Rosolowska J,Van Den Brande I,Pennewaert A,D'Hont K,Bossut M,Jantz D,Ruiter R,Broadhvest J

    更新日期:2013-10-01 00:00:00

  • MicroRNA156 as a promising tool for alfalfa improvement.

    abstract::A precursor of miR156 (MsmiR156d) was cloned and overexpressed in alfalfa (Medicago sativa L.) as a means to enhance alfalfa biomass yield. Of the five predicted SPL genes encoded by the alfalfa genome, three (SPL6, SPL12 and SPL13) contain miR156 cleavage sites and their expression was down-regulated in transgenic al...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Aung B,Gruber MY,Amyot L,Omari K,Bertrand A,Hannoufa A

    更新日期:2015-08-01 00:00:00

  • High level protein expression in plants through the use of a novel autonomously replicating geminivirus shuttle vector.

    abstract::We constructed a novel autonomously replicating gene expression shuttle vector, with the aim of developing a system for transiently expressing proteins at levels useful for commercial production of vaccines and other proteins in plants. The vector, pRIC, is based on the mild strain of the geminivirus Bean yellow dwarf...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Regnard GL,Halley-Stott RP,Tanzer FL,Hitzeroth II,Rybicki EP

    更新日期:2010-01-01 00:00:00

  • Increased expression of phospholipase Dα1 in guard cells decreases water loss with improved seed production under drought in Brassica napus.

    abstract::The activation of phospholipase Dα1 (PLDα1) produces lipid messenger phosphatidic acid and promotes stomatal closure in Arabidopsis. To explore the use of the PLDα1-mediated signalling towards decreasing water loss in crop plants, we introduced Arabidopsis PLDα1 under the control of a guard cell-specific promoter AtKa...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Lu S,Bahn SC,Qu G,Qin H,Hong Y,Xu Q,Zhou Y,Hong Y,Wang X

    更新日期:2013-04-01 00:00:00

  • Comparative transcriptomics as a tool for the identification of root branching genes in maize.

    abstract::The root system is fundamental for plant development, is crucial for overall plant growth and is recently being recognized as the key for future crop productivity improvement. A major determinant of root system architecture is the initiation of lateral roots. While knowledge of the genetic and molecular mechanisms reg...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Jansen L,Hollunder J,Roberts I,Forestan C,Fonteyne P,Van Quickenborne C,Zhen RG,McKersie B,Parizot B,Beeckman T

    更新日期:2013-12-01 00:00:00

  • Suspension-cultured BY-2 tobacco cells produce and mature immunologically active house dust mite allergens.

    abstract::The replacement of crude allergen extracts by selected allergens currently represents a major goal for the improvement of allergy diagnosis and immunotherapy. Indeed, the development of molecularly defined vaccines would facilitate both standardization and enhance batch-to-batch reproducibility as well as treatment sp...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Lienard D,Tran Dinh O,van Oort E,Van Overtvelt L,Bonneau C,Wambre E,Bardor M,Cosette P,Didier-Laurent A,de Borne FD,Delon R,van Ree R,Moingeon P,Faye L,Gomord V

    更新日期:2007-01-01 00:00:00

  • Discovery and development of exome-based, co-dominant single nucleotide polymorphism markers in hexaploid wheat (Triticum aestivum L.).

    abstract::Globally, wheat is the most widely grown crop and one of the three most important crops for human and livestock feed. However, the complex nature of the wheat genome has, until recently, resulted in a lack of single nucleotide polymorphism (SNP)-based molecular markers of practical use to wheat breeders. Recently, lar...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Allen AM,Barker GL,Wilkinson P,Burridge A,Winfield M,Coghill J,Uauy C,Griffiths S,Jack P,Berry S,Werner P,Melichar JP,McDougall J,Gwilliam R,Robinson P,Edwards KJ

    更新日期:2013-04-01 00:00:00

  • A maize phytochrome-interacting factors protein ZmPIF1 enhances drought tolerance by inducing stomatal closure and improves grain yield in Oryza sativa.

    abstract::Phytochrome-interacting factors (PIFs) play major roles in regulating plant growth and development, but their roles in drought stress remain elusive. Here, we cloned and characterized a maize (Zea mays) PIF transcription factor, ZmPIF1. The expression level of ZmPIF1 was significantly induced by independent drought an...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Gao Y,Wu M,Zhang M,Jiang W,Ren X,Liang E,Zhang D,Zhang C,Xiao N,Li Y,Dai Y,Chen J

    更新日期:2018-07-01 00:00:00

  • Screening for resistance against Pseudomonas syringae in rice-FOX Arabidopsis lines identified a putative receptor-like cytoplasmic kinase gene that confers resistance to major bacterial and fungal pathogens in Arabidopsis and rice.

    abstract::Approximately 20,000 of the rice-FOX Arabidopsis transgenic lines, which overexpress 13,000 rice full-length cDNAs at random in Arabidopsis, were screened for bacterial disease resistance by dip inoculation with Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). The identities of the overexpressed genes were determi...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Dubouzet JG,Maeda S,Sugano S,Ohtake M,Hayashi N,Ichikawa T,Kondou Y,Kuroda H,Horii Y,Matsui M,Oda K,Hirochika H,Takatsuji H,Mori M

    更新日期:2011-05-01 00:00:00

  • Genetic and metabolic effects of ripening mutations and vine detachment on tomato fruit quality.

    abstract::Tomato (Solanum lycopersicum) fruit ripening is regulated co-operatively by the action of ethylene and a hierarchy of transcription factors, including RIPENING INHIBITOR (RIN) and NON-RIPENING (NOR). Mutations in these two genes have been adopted commercially to delay ripening, and accompanying textural deterioration,...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Osorio S,Carneiro RT,Lytovchenko A,McQuinn R,Sørensen I,Vallarino JG,Giovannoni JJ,Fernie AR,Rose JKC

    更新日期:2020-01-01 00:00:00

  • Highly rearranged and size-variable chloroplast genomes in conifers II clade (cupressophytes): evolution towards shorter intergenic spacers.

    abstract::Although conifers are of immense ecological and economic value, bioengineering of their chloroplasts remains undeveloped. Understanding the chloroplast genomic organization of conifers can facilitate their bioengineering. Members of the conifer II clade (or cupressophytes) are highly diverse in both morphologic featur...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Wu CS,Chaw SM

    更新日期:2014-04-01 00:00:00

  • The impact of secondary pests on Bacillus thuringiensis (Bt) crops.

    abstract::The intensification of agriculture and the development of synthetic insecticides enabled worldwide grain production to more than double in the last third of the 20th century. However, the heavy dependence and, in some cases, overuse of insecticides has been responsible for negative environmental and ecological impacts...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审


    authors: Catarino R,Ceddia G,Areal FJ,Park J

    更新日期:2015-06-01 00:00:00

  • High-Spatiotemporal-Resolution Transcriptomes Provide Insights into Fruit Development and Ripening in Citrus sinensis.

    abstract::Citrus fruit has a unique structure with soft leathery peel and pulp containing vascular bundles and several segments with many juice sacs. The function and morphology of each fruit tissue are different. Therefore, analysis at the organ-wide or mixed-tissue level inevitably obscures many tissue-specific phenomena. Hig...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Feng G,Wu J,Xu Y,Lu L,Yi H

    更新日期:2021-01-20 00:00:00

  • The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants.

    abstract::Reverse transcription-polymerase chain reaction (RT-PCR) approaches have been used in a large proportion of transcriptome analyses published to date. The accuracy of the results obtained by this method strongly depends on accurate transcript normalization using stably expressed genes, known as references. Statistical ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Gutierrez L,Mauriat M,Guénin S,Pelloux J,Lefebvre JF,Louvet R,Rusterucci C,Moritz T,Guerineau F,Bellini C,Van Wuytswinkel O

    更新日期:2008-08-01 00:00:00

  • Stacking three late blight resistance genes from wild species directly into African highland potato varieties confers complete field resistance to local blight races.

    abstract::Considered responsible for one million deaths in Ireland and widespread famine in the European continent during the 1840s, late blight, caused by Phytophthora infestans, remains the most devastating disease of potato (Solanum tuberosum L.) with about 15%-30% annual yield loss in sub-Saharan Africa, affecting mainly sm...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Ghislain M,Byarugaba AA,Magembe E,Njoroge A,Rivera C,Román ML,Tovar JC,Gamboa S,Forbes GA,Kreuze JF,Barekye A,Kiggundu A

    更新日期:2019-06-01 00:00:00

  • Resistance and biomass in Arabidopsis: a new model for salicylic acid perception.

    abstract::Salicylic acid (SA) is an essential hormone for plant defence and development. SA perception is usually measured by counting the number of pathogens that grow in planta upon an exogenous application of the hormone. A biological SA perception model based on plant fresh weight reduction caused by disease resistance in A...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Canet JV,Dobón A,Ibáñez F,Perales L,Tornero P

    更新日期:2010-02-01 00:00:00

  • Production of recombinant human granulocyte macrophage-colony stimulating factor in rice cell suspension culture with a human-like N-glycan structure.

    abstract::The rice α-amylase 3D promoter system, which is activated under sucrose-starved conditions, has emerged as a useful system for producing recombinant proteins. However, using rice as the production system for therapeutic proteins requires modifications of the N-glycosylation pattern because of the potential immunogenic...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Shin YJ,Chong YJ,Yang MS,Kwon TH

    更新日期:2011-12-01 00:00:00

  • Oligonucleotide-directed mutagenesis for precision gene editing.

    abstract::Differences in gene sequences, many of which are single nucleotide polymorphisms, underlie some of the most important traits in plants. With humanity facing significant challenges to increase global agricultural productivity, there is an urgent need to accelerate the development of these traits in plants. oligonucleot...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章,评审


    authors: Sauer NJ,Mozoruk J,Miller RB,Warburg ZJ,Walker KA,Beetham PR,Schöpke CR,Gocal GF

    更新日期:2016-02-01 00:00:00

  • Trans-species synthetic gene design allows resistance pyramiding and broad-spectrum engineering of virus resistance in plants.

    abstract::To infect plants, viruses rely heavily on their host's machinery. Plant genetic resistances based on host factor modifications can be found among existing natural variability and are widely used for some but not all crops. While biotechnology can supply for the lack of natural resistance alleles, new strategies need t...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Bastet A,Lederer B,Giovinazzo N,Arnoux X,German-Retana S,Reinbold C,Brault V,Garcia D,Djennane S,Gersch S,Lemaire O,Robaglia C,Gallois JL

    更新日期:2018-03-05 00:00:00

  • Genetic modification of cassava for enhanced starch production.

    abstract::To date, transgenic approaches to biofortify subsistence crops have been rather limited. This is particularly true for the starchy root crop cassava (Manihot esculenta Crantz). Cassava has one of the highest rates of CO(2) fixation and sucrose synthesis for any C3 plant, but rarely reaches its yield potentials in the ...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Ihemere U,Arias-Garzon D,Lawrence S,Sayre R

    更新日期:2006-07-01 00:00:00

  • Synthesis of bacteriophage lytic proteins against Streptococcus pneumoniae in the chloroplast of Chlamydomonas reinhardtii.

    abstract::There is a pressing need to develop novel antibacterial agents given the widespread antibiotic resistance among pathogenic bacteria and the low specificity of the drugs available. Endolysins are antibacterial proteins that are produced by bacteriophage-infected cells to digest the bacterial cell wall for phage progeny...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Stoffels L,Taunt HN,Charalambous B,Purton S

    更新日期:2017-09-01 00:00:00

  • Application of two bicistronic systems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm.

    abstract::Coordination of multiple transgenes is essential for metabolic engineering of biosynthetic pathways. Here, we report the utilization of two bicistronic systems involving the 2A sequence from the foot-and-mouth disease virus and the internal ribosome entry site (IRES) sequence from the crucifer-infecting tobamovirus to...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Ha SH,Liang YS,Jung H,Ahn MJ,Suh SC,Kweon SJ,Kim DH,Kim YM,Kim JK

    更新日期:2010-10-01 00:00:00

  • Enhancing the expression of starch synthase class IV results in increased levels of both transitory and long-term storage starch.

    abstract::Starch is an important renewable raw material with an increasing number of applications. Several attempts have been made to obtain plants that produce modified versions of starch or higher starch yield. Most of the approaches designed to increase the levels of starch have focused on the increment of the amount of ADP-...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Gámez-Arjona FM,Li J,Raynaud S,Baroja-Fernández E,Muñoz FJ,Ovecka M,Ragel P,Bahaji A,Pozueta-Romero J,Mérida Á

    更新日期:2011-12-01 00:00:00

  • Deficiency in the amino aldehyde dehydrogenase encoded by GmAMADH2, the homologue of rice Os2AP, enhances 2-acetyl-1-pyrroline biosynthesis in soybeans (Glycine max L.).

    abstract::2-Acetyl-1-pyrroline (2AP), the volatile compound that provides the 'popcorn-like' aroma in a large variety of cereal and food products, is widely found in nature. Deficiency in amino aldehyde dehydrogenase (AMADH) was previously shown to be the likely cause of 2AP biosynthesis in rice (Oryza sativa L.). In this study...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Arikit S,Yoshihashi T,Wanchana S,Uyen TT,Huong NT,Wongpornchai S,Vanavichit A

    更新日期:2011-01-01 00:00:00

  • Induction and recovery of copy number variation in banana through gamma irradiation and low-coverage whole-genome sequencing.

    abstract::Traditional breeding methods are hindered in bananas due to the fact that major cultivars are sterile, parthenocarpic, triploid and thus clonally propagated. This has resulted in a narrow genetic base and limited resilience to biotic and abiotic stresses. Mutagenesis of in vitro propagated bananas is one method to int...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Datta S,Jankowicz-Cieslak J,Nielen S,Ingelbrecht I,Till BJ

    更新日期:2018-02-24 00:00:00

  • System analysis of microRNAs in the development and aluminium stress responses of the maize root system.

    abstract::MicroRNAs (miRNAs) are a class of regulatory small RNAs (sRNAs) that down-regulate target genes through mRNA cleavage or translational inhibition. miRNA is known to play an important role in the root development and environmental responses in both the Arabidopsis and rice. However, little information is available to f...

    journal_title:Plant biotechnology journal

    pub_type: 杂志文章


    authors: Kong X,Zhang M,Xu X,Li X,Li C,Ding Z

    更新日期:2014-10-01 00:00:00