Engineering canker-resistant plants through CRISPR/Cas9-targeted editing of the susceptibility gene CsLOB1 promoter in citrus.

abstract：:Transgenic engineering of plants is important in both basic and applied research. However, the expression of a transgene can dwindle over time as the plant's small (s)RNA-guided silencing pathways shut it down. The silencing pathways have evolved as antiviral defence mechanisms, and viruses have co-evolved viral silen...

journal_title：Plant biotechnology journal

authors： Naim F,Shrestha P,Singh SP,Waterhouse PM,Wood CC

更新日期：2016-06-01 00:00:00

abstract：:Mutation discovery technologies have enabled the development of reverse genetics for many plant species and allowed sophisticated evaluation of the consequences of mutagenesis. Such methods are relatively straightforward for seed-propagated plants. To develop a platform suitable for vegetatively propagated species, we...

journal_title：Plant biotechnology journal

authors： Jankowicz-Cieslak J,Huynh OA,Brozynska M,Nakitandwe J,Till BJ

更新日期：2012-12-01 00:00:00

abstract：:Recent developments of tools for targeted genome modification have led to new concepts in how multiple traits can be combined. Targeted genome modification is based on the use of nucleases with tailor-made specificities to introduce a DNA double-strand break (DSB) at specific target loci. A re-engineered meganuclease ...

journal_title：Plant biotechnology journal

authors： D'Halluin K,Vanderstraeten C,Van Hulle J,Rosolowska J,Van Den Brande I,Pennewaert A,D'Hont K,Bossut M,Jantz D,Ruiter R,Broadhvest J

更新日期：2013-10-01 00:00:00

abstract：:The importance and urgency of providing humans and animals with quality proteins are reflected in the growing scientific and industrial interest in augmenting the nutritive value of the world's protein sources. Such nutritive value is determined by the protein content in 'essential amino acids', those that cannot be s...

journal_title：Plant biotechnology journal

authors： Beauregard M,Hefford MA

更新日期：2006-09-01 00:00:00

abstract：:Glycine betaine plays an important role in some plants, including maize, in conditions of abiotic stress, but different maize varieties vary in their capacity to accumulate glycine betaine. An elite maize inbred line DH4866 was transformed with the betA gene from Escherichia coli encoding choline dehydrogenase (EC 1.1...

journal_title：Plant biotechnology journal

authors： Quan R,Shang M,Zhang H,Zhao Y,Zhang J

更新日期：2004-11-01 00:00:00

abstract：:Clustered regularly interspaced short palindromic repeat (CRISPR) and Cas9-associated protein systems provide a powerful genetic manipulation tool that can drive plant research forward. Nuclease-dead Cas9 (dCas9) is an enzymatically inactive mutant of Cas9 in which its endonuclease activity is non-functional. The appl...

journal_title：Plant biotechnology journal

authors： Moradpour M,Abdulah SNA

更新日期：2020-01-01 00:00:00

abstract：:The plant pathogen, Pseudomonas syringae (Ps), together with related Ps species, infects and attacks a wide range of agronomically important crops, including tomato, kiwifruit, pepper, olive and soybean, causing economic losses. Currently, chemicals and introduced resistance genes are used to protect plants against th...

journal_title：Plant biotechnology journal

authors： Rooney WM,Grinter RW,Correia A,Parkhill J,Walker DC,Milner JJ

更新日期：2020-05-01 00:00:00

abstract：:Drought is the major environmental factor limiting crop productivity worldwide. We hypothesized that it is possible to enhance drought tolerance by delaying stress-induced senescence through the stress-induced synthesis of cytokinins in crop-plants. We generated transgenic rice (Oryza sativa) plants expressing an isop...

journal_title：Plant biotechnology journal

authors： Peleg Z,Reguera M,Tumimbang E,Walia H,Blumwald E

更新日期：2011-09-01 00:00:00

abstract：:Although much explored, oral tolerance for treatment of autoimmune diseases still awaits the establishment of novel and effective vectors. We investigated whether the tolerogenic CTA1(R7K)-COL-DD fusion protein can be expressed in edible plants, to induce oral tolerance and protect against arthritis. The fusion protei...

journal_title：Plant biotechnology journal

authors： Hansson C,Schön K,Kalbina I,Strid Å,Andersson S,Bokarewa MI,Lycke NY

更新日期：2016-04-01 00:00:00

abstract：:Salt stress has detrimental effects on crop growth and yield, and the area of salt-affected land is increasing. Soybean is a major source of vegetable protein, oil and feed, but considered as a salt-sensitive crop. Cultivated soybean (Glycine max) is domesticated from wild soybean (G. soja) but lost considerable amoun...

journal_title：Plant biotechnology journal

authors： Jin T,Sun Y,Shan Z,He J,Wang N,Gai J,Li Y

更新日期：2020-12-25 00:00:00

abstract：:Drought tolerance of the wheat cultivar Bobwhite was previously enhanced by transformation with a construct containing the wheat DREB3 gene driven by the stress-inducible maize Rab17 promoter. Progeny of a single T2 transgenic line were used as pollinators in crosses with four elite bread wheat cultivars from Western ...

journal_title：Plant biotechnology journal

authors： Shavrukov Y,Baho M,Lopato S,Langridge P

更新日期：2016-01-01 00:00:00

abstract：:Maize kernels do not contain enough of the essential sulphur-amino acid methionine (Met) to serve as a complete diet for animals, even though maize has the genetic capacity to store Met in kernels. Prior studies indicated that the availability of the sulphur (S)-amino acids may limit their incorporation into seed stor...

journal_title：Plant biotechnology journal

authors： Xiang X,Wu Y,Planta J,Messing J,Leustek T

更新日期：2018-05-01 00:00:00

abstract：:Soya bean (Glycine max) and grass pea (Lathyrus sativus) seeds are important sources of dietary proteins; however, they also contain antinutritional metabolite oxalic acid (OA). Excess dietary intake of OA leads to nephrolithiasis due to the formation of calcium oxalate crystals in kidneys. Besides, OA is also a known...

journal_title：Plant biotechnology journal

authors： Kumar V,Chattopadhyay A,Ghosh S,Irfan M,Chakraborty N,Chakraborty S,Datta A

更新日期：2016-06-01 00:00:00

abstract：:The root system is fundamental for plant development, is crucial for overall plant growth and is recently being recognized as the key for future crop productivity improvement. A major determinant of root system architecture is the initiation of lateral roots. While knowledge of the genetic and molecular mechanisms reg...

journal_title：Plant biotechnology journal

authors： Jansen L,Hollunder J,Roberts I,Forestan C,Fonteyne P,Van Quickenborne C,Zhen RG,McKersie B,Parizot B,Beeckman T

更新日期：2013-12-01 00:00:00

abstract：:Approximately 20,000 of the rice-FOX Arabidopsis transgenic lines, which overexpress 13,000 rice full-length cDNAs at random in Arabidopsis, were screened for bacterial disease resistance by dip inoculation with Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). The identities of the overexpressed genes were determi...

journal_title：Plant biotechnology journal

authors： Dubouzet JG,Maeda S,Sugano S,Ohtake M,Hayashi N,Ichikawa T,Kondou Y,Kuroda H,Horii Y,Matsui M,Oda K,Hirochika H,Takatsuji H,Mori M

更新日期：2011-05-01 00:00:00

abstract：:GM crops are the most studied crops in history. Approximately 5% of the safety studies on them show adverse effects that are a cause for concern and tend to be featured in media reports. Although these reports are based on just a handful of GM events, they are used to cast doubt on all GM crops. Furthermore, they tend...

journal_title：Plant biotechnology journal

authors： Sánchez MA,Parrott WA

更新日期：2017-10-01 00:00:00

abstract：:The rice α-amylase 3D promoter system, which is activated under sucrose-starved conditions, has emerged as a useful system for producing recombinant proteins. However, using rice as the production system for therapeutic proteins requires modifications of the N-glycosylation pattern because of the potential immunogenic...

journal_title：Plant biotechnology journal

authors： Shin YJ,Chong YJ,Yang MS,Kwon TH

更新日期：2011-12-01 00:00:00

abstract：:The activation of phospholipase Dα1 (PLDα1) produces lipid messenger phosphatidic acid and promotes stomatal closure in Arabidopsis. To explore the use of the PLDα1-mediated signalling towards decreasing water loss in crop plants, we introduced Arabidopsis PLDα1 under the control of a guard cell-specific promoter AtKa...

journal_title：Plant biotechnology journal

authors： Lu S,Bahn SC,Qu G,Qin H,Hong Y,Xu Q,Zhou Y,Hong Y,Wang X

更新日期：2013-04-01 00:00:00

abstract：:Glyphosate is a popular, systemic, broad-spectrum herbicide used in modern agriculture. Being a structural analog of phosphoenolpyruvate (PEP), it inhibits 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) which is responsible for the biosynthesis of aromatic amino acids and various aromatic secondary metabolites. T...

journal_title：Plant biotechnology journal

authors： Achary VMM,Sheri V,Manna M,Panditi V,Borphukan B,Ram B,Agarwal A,Fartyal D,Teotia D,Masakapalli SK,Agrawal PK,Reddy MK

更新日期：2020-12-01 00:00:00

abstract：:Following the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing (dRenSeq) enables the high-confidence i...

journal_title：Plant biotechnology journal

authors： Armstrong MR,Vossen J,Lim TY,Hutten RCB,Xu J,Strachan SM,Harrower B,Champouret N,Gilroy EM,Hein I

更新日期：2019-02-01 00:00:00

abstract：:Peanut allergy is an IgE-mediated adverse reaction to a subset of proteins found in peanuts. Immunotherapy aims to desensitize allergic patients through repeated and escalating exposures for several months to years using extracts or flours. The complex mix of proteins and variability between preparations complicates i...

journal_title：Plant biotechnology journal

authors： Gregory JA,Shepley-McTaggart A,Umpierrez M,Hurlburt BK,Maleki SJ,Sampson HA,Mayfield SP,Berin MC

更新日期：2016-07-01 00:00:00

abstract：:Dioxin residues widely contaminate soil and agricultural products at low concentrations and may accumulate in organisms at the top of food chains owing to their physicochemical properties. In this study, we have developed novel, dioxin-inducible, reporter gene expression systems regulated by recombinant aryl hydrocarb...

journal_title：Plant biotechnology journal

authors： Kodama S,Okada K,Akimoto K,Inui H,Ohkawa H

更新日期：2009-02-01 00:00:00

abstract：:The related A genome species of the Oryza genus are the effective gene pool for rice. Here, we report draft genomes for two Australian wild A genome taxa: O. rufipogon-like population, referred to as Taxon A, and O. meridionalis-like population, referred to as Taxon B. These two taxa were sequenced and assembled by in...

journal_title：Plant biotechnology journal

authors： Brozynska M,Copetti D,Furtado A,Wing RA,Crayn D,Fox G,Ishikawa R,Henry RJ

更新日期：2017-06-01 00:00:00

abstract：:An important goal in biotechnological research is to improve the yield of crop plants. Here, we genetically modified simultaneously source and sink capacities in potato (Solanum tuberosum cv. Desirée) plants to improve starch yield. Source capacity was increased by mesophyll-specific overexpression of a pyrophosphatas...

journal_title：Plant biotechnology journal

authors： Jonik C,Sonnewald U,Hajirezaei MR,Flügge UI,Ludewig F

更新日期：2012-12-01 00:00:00

abstract：:Soybean cyst nematode (SCN) is the most devastating plant-parasitic nematode. Most commercial soybean varieties with SCN resistance are derived from PI88788. Resistance derived from PI88788 is breaking down due to narrow genetic background and SCN population shift. PI88788 requires mainly the rhg1-b locus, while 'Peki...

journal_title：Plant biotechnology journal

authors： Patil GB,Lakhssassi N,Wan J,Song L,Zhou Z,Klepadlo M,Vuong TD,Stec AO,Kahil SS,Colantonio V,Valliyodan B,Rice JH,Piya S,Hewezi T,Stupar RM,Meksem K,Nguyen HT

更新日期：2019-08-01 00:00:00

abstract：:The early methionine (Em) proteins are members of the late embryogenesis abundant (LEA) group of proteins that have been considered to be embryo specific. The ability of a 646-bp wheat Em promoter to control green fluorescent protein (gfp) expression was investigated in transgenic barley and rice. Seeds of transgenic ...

journal_title：Plant biotechnology journal

authors： Furtado A,Henry RJ

更新日期：2005-07-01 00:00:00

abstract：:Heavy metal accumulation in the environment poses great risks to flora and fauna. However, monitoring sites prone to accumulation poses scale and economic challenges. In this study, we present and test a method for monitoring these sites using fluorescent resonance energy transfer (FRET) change in response to zinc (Zn...

journal_title：Plant biotechnology journal

authors： Adams JP,Adeli A,Hsu CY,Harkess RL,Page GP,Depamphilis CW,Schultz EB,Yuceer C

更新日期：2012-02-01 00:00:00

abstract：:S-adenosyl-L-methionine (SAM) synthetase is the key enzyme involved in the biosynthesis of SAM, which serves as a common precursor for polyamines (PAs) and ethylene. A SAM synthetase cDNA (SlSAMS1) was introduced into the tomato genome using the Agrobacterium tumefaciens transformation method. Transgenic plants overex...

journal_title：Plant biotechnology journal

authors： Gong B,Li X,VandenLangenberg KM,Wen D,Sun S,Wei M,Li Y,Yang F,Shi Q,Wang X

更新日期：2014-08-01 00:00:00

abstract：:Porcine circovirus type 2 (PCV-2) is the main causative agent associated with a group of diseases collectively known as porcine circovirus-associated disease (PCAD). There is a significant economic strain on the global swine industry due to PCAD and the production of commercial PCV-2 vaccines is expensive. Plant expre...

journal_title：Plant biotechnology journal

authors： Gunter CJ,Regnard GL,Rybicki EP,Hitzeroth II

更新日期：2019-09-01 00:00:00

abstract：:Zinc-finger nucleases (ZFNs) are artificial restriction enzymes, custom designed for induction of double-strand breaks (DSBs) at a specific locus. These DSBs may result in site-specific mutagenesis or homologous recombination at the repair site, depending on the DNA repair pathway that is used. These promising techniq...

journal_title：Plant biotechnology journal

authors： de Pater S,Neuteboom LW,Pinas JE,Hooykaas PJ,van der Zaal BJ

更新日期：2009-10-01 00:00:00