Abstract:
:Direct control of protein level enables rapid and efficient analyses of gene functions in crops. Previously, we developed the RDDK-Shield1 (Shld1) system in the model plant Arabidopsis thaliana for direct modulation of protein stabilization using a synthetic small molecule. However, it was unclear whether this system is applicable to economically important crops. In this study, we show that the RDDK-Shld1 system enables rapid and tunable control of protein levels in rice and wheat. Accumulation of RDDK fusion proteins can be reversibly and spatio-temporally controlled by the synthetic small-molecule Shld1. Moreover, RDDK-Bar and RDDK-Pid3 fusions confer herbicide and rice blast resistance, respectively, in a Shld1-dependent manner. Therefore, the RDDK-Shld1 system provides a reversible and tunable technique for controlling protein functions and conditional expression of transgenes in crops.
journal_name
Plant Biotechnol Jjournal_title
Plant biotechnology journalauthors
Zhang J,Yin K,Sun J,Gao J,Du Q,Li H,Qiu JLdoi
10.1111/pbi.12787subject
Has Abstractpub_date
2018-02-01 00:00:00pages
472-481issue
2eissn
1467-7644issn
1467-7652journal_volume
16pub_type
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